Skip Header

Contribute Send feedback
Read comments (?) or add your own

P38166 (SFT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein transport protein SFT2
Gene names
Name:SFT2
Ordered Locus Names:YBL102W
ORF Names:YBL0812
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length215 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Nonessential protein required for the fusion of transport vesicles derived from the endocytic pathway with the Golgi complex. Can be partially replaced by GOT1. Ref.6

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein Ref.6.

Disruption phenotype

Lack of SFT2 is lethal in a strain lacking GOT1. Ref.6

Miscellaneous

Present with 279 molecules/cell in log phase SD medium. Ref.7

Sequence similarities

Belongs to the SFT2 family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentGolgi apparatus
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processGolgi to endosome transport

Inferred from mutant phenotype. Source: SGD

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentGolgi membrane

Inferred from direct assay. Source: SGD

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 215215Protein transport protein SFT2
PRO_0000097713

Regions

Topological domain1 – 8181Cytoplasmic Potential
Transmembrane82 – 10221Helical; Name=1; Potential
Topological domain103 – 1119Lumenal Potential
Transmembrane112 – 13221Helical; Name=2; Potential
Topological domain133 – 14513Cytoplasmic Potential
Transmembrane146 – 16621Helical; Name=3; Potential
Topological domain167 – 1726Lumenal Potential
Transmembrane173 – 19321Helical; Name=4; Potential
Topological domain194 – 21522Cytoplasmic Potential

Amino acid modifications

Modified residue601Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P38166 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 1BF971749814382C

FASTA21524,274
        10         20         30         40         50         60 
MSEEPPSDQV NSLRDSLNRW NQTRQQNSQG FNESAKTLFS SWADSLNTRA QDIYQTLPVS 

        70         80         90        100        110        120 
RQDLVQDQEP SWFQLSRTER MVLFVCFLLG ATACFTLCTF LFPVLAAKPR KFGLLWTMGS 

       130        140        150        160        170        180 
LLFVLAFGVL MGPLAYLKHL TARERLPFSM FFFATCFMTI YFAAFSKNTV LTITCALLEL 

       190        200        210 
VAVIYYAISY FPFGATGLRM LSSAGVNSAR GVLRI

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"A SNARE-like protein required for traffic through the Golgi complex."
Banfield D.K., Lewis M.J., Pelham H.R.B.
Nature 375:806-809(1995) [PubMed: 7596416] [Abstract]
Cited for: IDENTIFICATION.
[6]"Got1p and Sft2p: membrane proteins involved in traffic to the Golgi complex."
Conchon S., Cao X., Barlowe C., Pelham H.R.
EMBO J. 18:3934-3946(1999) [PubMed: 10406798] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, DISRUPTION PHENOTYPE.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X79489 Genomic DNA. Translation: CAA55993.1.
Z35863 Genomic DNA. Translation: CAA84928.1.
AY558550 Genomic DNA. Translation: AAS56876.1.
BK006936 Genomic DNA. Translation: DAA07021.1.
PIRS45393.
RefSeqNP_009448.1. NM_001178342.1.

3D structure databases

ProteinModelPortalP38166.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1865N.
IntActP38166. 28 interactions.
MINTMINT-406787.
STRINGP38166.

Proteomic databases

PeptideAtlasP38166.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL102W; YBL102W; YBL102W.
GeneID852172.
KEGGsce:YBL102W.
NMPDRfig|4932.3.peg.133.

Organism-specific databases

CYGDYBL102w.
SGDS000000198. SFT2.

Phylogenomic databases

eggNOGfuNOG10201.
GeneTreeEFGT00050000006436.
HOGENOMHBG323920.
OMAPRKFGLL.
OrthoDBEOG4X9BSQ.

Gene expression databases

ArrayExpressP38166.
GenevestigatorP38166.
GermOnlineYBL102W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR007305. Vesicle_transpt_Got1/SFT2.
IPR011691. Vesicle_transpt_SFT2.
[Graphical view]
PANTHERPTHR23137. SFT2. 1 hit.
PfamPF04178. Got1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970623.

Entry information

Entry nameSFT2_YEAST
AccessionPrimary (citable) accession number: P38166
Secondary accession number(s): D6VPQ1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents