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P38165 (RTG3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Retrograde regulation protein 3
Gene names
Name:RTG3
Ordered Locus Names:YBL103C
ORF Names:YBL0810
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length486 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription factor that regulates CIT2 gene expression. Binds to two identical sites oriented as inverted repeats 28 bp apart in a regulatory upstream activation sequence element (UASR) in the CIT2 promoter. The core binding site is 5'-GGTCAC-3'.

Subunit structure

Binds DNA as a heterodimer with RTG1.

Subcellular location

Nucleus Probable.

Domain

the 9aaTAD motifs are transactivation domains present in a large number of yeast and animal transcription factors. Ref.6

Miscellaneous

Present with 1050 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.

Sequence caution

The sequence CAA55992.1 differs from that shown. Reason: Frameshift at position 310.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RTG1P326074EBI-16328,EBI-16312

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 486486Retrograde regulation protein 3
PRO_0000127434

Regions

Domain299 – 34547Helix-loop-helix motif
DNA binding283 – 29816Basic motif
Motif27 – 3599aaTAD 1
Motif189 – 19799aaTAD 2

Amino acid modifications

Modified residue721Phosphoserine Ref.9
Modified residue1131Phosphoserine Ref.9
Modified residue1231Phosphoserine Ref.7 Ref.9
Modified residue1261Phosphoserine Ref.9
Modified residue1351Phosphoserine Ref.9
Modified residue1401Phosphotyrosine Ref.9
Modified residue1421Phosphoserine Ref.7 Ref.9
Modified residue1501Phosphothreonine Ref.9
Modified residue2211Phosphotyrosine Ref.9
Modified residue2221Phosphoserine Ref.9
Modified residue2271Phosphoserine Ref.8 Ref.9
Modified residue2361Phosphoserine Ref.9
Modified residue2791Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P38165 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: DB635FFDEC740C11

FASTA48654,140
        10         20         30         40         50         60 
MMNNNESEAE NQRLLDELMN QTKVLQETLD FSLVTPTPHH NDDYKIHGSA YPGGETPAQQ 

        70         80         90        100        110        120 
HEKLSYINTH NSNDNNNLMG SQARSNSQTP TASTIYEEAE SQSSYLDDMF RTSQGGRPVT 

       130        140        150        160        170        180 
QNSISSIGQG PLRSSYSMAY DSPVDRAMNT PLQQQEGLKA ELPHDFLFQH GTDDTMYNLT 

       190        200        210        220        230        240 
DDLSSSLSSS INSDMMTPNT YSSSFSYNPQ SLGPASVSST YSPKVRSPSS SFRAGSFLSS 

       250        260        270        280        290        300 
SFRHGSINTP RTRHTSISSN MTENIGPGSV PKILGGLTSD EKLRRKREFH NAVERRRREL 

       310        320        330        340        350        360 
IKQKIKELGQ LVPPSLLNYD DLGKQIKPNK GIILDRTVEY LQYLAEILEI QARKKKALLA 

       370        380        390        400        410        420 
KIKELEEKKS SVAALSPFTN NHHASSGQNN SENSEERIID IRSVPNALMN EQNSKAELHN 

       430        440        450        460        470        480 
WEPPLYDSVG NHNHAGTMES HPHTNIHEEL KEFLSGDLIE AEDNAKLMFG DDNSNPADYL 


LEFGSG 

« Hide

References

« Hide 'large scale' references
[1]"A basic helix-loop-helix-leucine zipper transcription complex in yeast functions in a signaling pathway from mitochondria to the nucleus."
Jia Y., Rothermel B., Thornton J., Butow R.A.
Mol. Cell. Biol. 17:1110-1117(1997) [PubMed: 9032238] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / GRF88.
[2]"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Nine-amino-acid transactivation domain: establishment and prediction utilities."
Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M.
Genomics 89:756-768(2007) [PubMed: 17467953] [Abstract]
Cited for: DOMAIN.
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-123 AND SER-142, MASS SPECTROMETRY.
Strain: ADR376.
[8]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227, MASS SPECTROMETRY.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; SER-113; SER-123; SER-126; SER-135; TYR-140; SER-142; THR-150; TYR-221; SER-222; SER-227; SER-236 AND SER-279, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U46012 Genomic DNA. Translation: AAA86842.1.
X79489 Genomic DNA. Translation: CAA55992.1. Frameshift.
Z35864 Genomic DNA. Translation: CAA84929.1.
Z35865 Genomic DNA. Translation: CAA84931.1.
BK006936 Genomic DNA. Translation: DAA07020.1.
PIRS74319.
RefSeqNP_009447.1. NM_001178343.1.

3D structure databases

ProteinModelPortalP38165.
SMRP38165. Positions 284-346.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-2501N.
IntActP38165. 22 interactions.
MINTMINT-1555168.
STRINGP38165.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL103C; YBL103C; YBL103C.
GeneID852171.
KEGGsce:YBL103C.
NMPDRfig|4932.3.peg.132.

Organism-specific databases

CYGDYBL103c.
SGDS000000199. RTG3.

Phylogenomic databases

eggNOGfuNOG11021.
OMASENSEER.
OrthoDBEOG4D82GC.

Gene expression databases

ArrayExpressP38165.
GenevestigatorP38165.
GermOnlineYBL103C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR024097. bHLH_ZIP_TF.
IPR011598. HLH_DNA-bd.
IPR024099. TF_RTG3.
[Graphical view]
Gene3DG3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
PANTHERPTHR10014. PTHR10014. 1 hit.
PTHR10014:SF6. PTHR10014:SF6. 1 hit.
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. HLH_basic. 1 hit.
PROSITEPS50888. HLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970620.

Entry information

Entry nameRTG3_YEAST
AccessionPrimary (citable) accession number: P38165
Secondary accession number(s): D6VPQ0, P89494
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: December 14, 2011
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

SIMILARITY comments

Index of protein domains and families