P38165 (RTG3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Retrograde regulation protein 3

Gene names

Name:	RTG3
Ordered Locus Names:	YBL103C
ORF Names:	YBL0810

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor that regulates CIT2 gene expression. Binds to two identical sites oriented as inverted repeats 28 bp apart in a regulatory upstream activation sequence element (UASR) in the CIT2 promoter. The core binding site is 5'-GGTCAC-3'.
Subunit structure	Binds DNA as a heterodimer with RTG1.
Subcellular location	Nucleus Probable.
Domain	the 9aaTAD motifs are transactivation domains present in a large number of yeast and animal transcription factors. Ref.6
Miscellaneous	Present with 1050 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain.
Sequence caution	The sequence CAA55992.1 differs from that shown. Reason: Frameshift at position 310.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondria-nucleus signaling pathway Inferred from direct assay Ref.1. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription initiation from RNA polymerase II promoter Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RTG1	P32607	4	EBI-16328,EBI-16312

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Retrograde regulation protein 3		PRO_0000127434
Regions
	Domain	299 – 345	47	Helix-loop-helix motif
	DNA binding	283 – 298	16	Basic motif
	Motif	27 – 35	9	9aaTAD 1
	Motif	189 – 197	9	9aaTAD 2
Amino acid modifications
	Modified residue	72	1	Phosphoserine Ref.9
	Modified residue	113	1	Phosphoserine Ref.9
	Modified residue	123	1	Phosphoserine Ref.7 Ref.9
	Modified residue	126	1	Phosphoserine Ref.9
	Modified residue	135	1	Phosphoserine Ref.9
	Modified residue	140	1	Phosphotyrosine Ref.9
	Modified residue	142	1	Phosphoserine Ref.7 Ref.9
	Modified residue	150	1	Phosphothreonine Ref.9
	Modified residue	221	1	Phosphotyrosine Ref.9
	Modified residue	222	1	Phosphoserine Ref.9
	Modified residue	227	1	Phosphoserine Ref.8 Ref.9
	Modified residue	236	1	Phosphoserine Ref.9
	Modified residue	279	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P38165 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: DB635FFDEC740C11	FASTA	486	54,140
	10 20 30 40 50 60 MMNNNESEAE NQRLLDELMN QTKVLQETLD FSLVTPTPHH NDDYKIHGSA YPGGETPAQQ 70 80 90 100 110 120 HEKLSYINTH NSNDNNNLMG SQARSNSQTP TASTIYEEAE SQSSYLDDMF RTSQGGRPVT 130 140 150 160 170 180 QNSISSIGQG PLRSSYSMAY DSPVDRAMNT PLQQQEGLKA ELPHDFLFQH GTDDTMYNLT 190 200 210 220 230 240 DDLSSSLSSS INSDMMTPNT YSSSFSYNPQ SLGPASVSST YSPKVRSPSS SFRAGSFLSS 250 260 270 280 290 300 SFRHGSINTP RTRHTSISSN MTENIGPGSV PKILGGLTSD EKLRRKREFH NAVERRRREL 310 320 330 340 350 360 IKQKIKELGQ LVPPSLLNYD DLGKQIKPNK GIILDRTVEY LQYLAEILEI QARKKKALLA 370 380 390 400 410 420 KIKELEEKKS SVAALSPFTN NHHASSGQNN SENSEERIID IRSVPNALMN EQNSKAELHN 430 440 450 460 470 480 WEPPLYDSVG NHNHAGTMES HPHTNIHEEL KEFLSGDLIE AEDNAKLMFG DDNSNPADYL LEFGSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A basic helix-loop-helix-leucine zipper transcription complex in yeast functions in a signaling pathway from mitochondria to the nucleus." Jia Y., Rothermel B., Thornton J., Butow R.A. Mol. Cell. Biol. 17:1110-1117(1997) [PubMed: 9032238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Nine-amino-acid transactivation domain: establishment and prediction utilities." Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M. Genomics 89:756-768(2007) [PubMed: 17467953] [Abstract] Cited for: DOMAIN.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-123 AND SER-142, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-227, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; SER-113; SER-123; SER-126; SER-135; TYR-140; SER-142; THR-150; TYR-221; SER-222; SER-227; SER-236 AND SER-279, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U46012 Genomic DNA. Translation: AAA86842.1. X79489 Genomic DNA. Translation: CAA55992.1. Frameshift. Z35864 Genomic DNA. Translation: CAA84929.1. Z35865 Genomic DNA. Translation: CAA84931.1. BK006936 Genomic DNA. Translation: DAA07020.1.
PIR	S74319.
RefSeq	NP_009447.1. NM_001178343.1.
3D structure databases
ProteinModelPortal	P38165.
SMR	P38165. Positions 284-346.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2501N.
IntAct	P38165. 22 interactions.
MINT	MINT-1555168.
STRING	P38165.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL103C; YBL103C; YBL103C.
GeneID	852171.
KEGG	sce:YBL103C.
NMPDR	fig|4932.3.peg.132.
Organism-specific databases
CYGD	YBL103c.
SGD	S000000199. RTG3.
Phylogenomic databases
eggNOG	fuNOG11021.
OMA	SENSEER.
OrthoDB	EOG4D82GC.
Gene expression databases
ArrayExpress	P38165.
Genevestigator	P38165.
GermOnline	YBL103C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR024097. bHLH_ZIP_TF. IPR011598. HLH_DNA-bd. IPR024099. TF_RTG3. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
PANTHER	PTHR10014. PTHR10014. 1 hit. PTHR10014:SF6. PTHR10014:SF6. 1 hit.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. HLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970620.

Entry information

Entry name

RTG3_YEAST

Accession

Primary (citable) accession number: P38165
Secondary accession number(s): D6VPQ0, P89494

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program