Reviewed, UniProtKB/Swiss-Prot P38164 (YBK4_YEAST)

Names and origin

Protein names

Recommended name:
    WD repeat-containing protein YBL104C

Gene names

Ordered Locus Names:	YBL104C/YBL103C-A
ORF Names:	YBL0808

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1038 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Cytoplasm.
Miscellaneous	Present with 184 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the WD repeat mio family. Contains 4 WD repeats.
Sequence caution	The sequence CAA55991.1 differs from that shown. Reason: Frameshift at positions 2, 7 and 977. The sequence CAA84930.1 differs from that shown. Reason: Frameshift at positions 2, 7 and 977.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Ref.5 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1038	1038	WD repeat-containing protein YBL104C		PRO_0000051470
Regions
	Repeat	50 – 90	41	WD 1
	Repeat	147 – 189	43	WD 2
	Repeat	235 – 276	42	WD 3
	Repeat	544 – 587	44	WD 4
Amino acid modifications
	Modified residue	123	1	Phosphoserine Ref.8
	Modified residue	136	1	Phosphoserine Ref.8
	Modified residue	476	1	Phosphoserine Ref.8
	Modified residue	895	1	Phosphoserine Ref.8
	Modified residue	901	1	Phosphoserine Ref.8
	Modified residue	902	1	Phosphothreonine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P38164-1 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 613630F9F41FF77C	FASTA	1,038	117,636
	10 20 30 40 50 60 MGLIKKVTHW SYDNLIDYLS VNPTRDEVTH YKVDPENESD ESIIKLHTVK DFGSITCLDY 70 80 90 100 110 120 SESEIGMIGV GEKNGYLRIF NISGQNSSSP ASHAPVGLNA NNETSMTNAS GGKAAQAENI 130 140 150 160 170 180 VGSVSNLKDT QGYPVSETNY DIRVRAKKQR CINSLGINTN GLIAMGLDRN KHDSSLQIWD 190 200 210 220 230 240 MNYHDDSHET INPMFSYCTN ESIVSLKFLN DTSVLAASTK FLKEIDVRSP NPIYQHPTRL 250 260 270 280 290 300 TYDIKLNPFN DWQFSTYGDD GTLAIWDRRK LSDQASLGDL NVASPLLTFE KLVGSGAASR 310 320 330 340 350 360 KYMNSCFRWS CVRNNEFATL HRGDTIKRWR LGYYCDSNRD IAADDDNEMN IENLFVSSVH 370 380 390 400 410 420 DTNTMYDRVA TFDYIPRSNN GTSLICMRQS GTIYRMPISE VCSKAILNNR NSLLLSNFEN 430 440 450 460 470 480 TEIDEIRVNN EHEKSNLENV KTILKNLSFE DLDVSEDYFP SGHDEPNNEI EYSELSEEEN 490 500 510 520 530 540 EGSNDVLDSK RGFELFWKPE KLLEKDISVI MRTRASLGYG LDPMNTVEMI DSSKNLQNNA 550 560 570 580 590 600 YIRNTWRWIA IAKASVDDGT MVSGDLDLGY EGVIGIWNGI NGISNQDRYR QETILSDKQL 610 620 630 640 650 660 NKEMEKIIKL RRKNRDRNSP IANAAGSPKY VQRRLCLIIS GWDLSRSDYE DKYNIIMKNG 670 680 690 700 710 720 HYEKAAAWAV FFGDIPKAVE ILGSAKKERL RLIATAIAGY LAYKDLPGNN AWRQQCRKMS 730 740 750 760 770 780 SELDDPYLRV IFAFIADNDW WDILYEPAIS LRERLGVALR FLNDTDLTTF LDRTSSTVIE 790 800 810 820 830 840 NGELEGLILT GITPNGIDLL QSYVNKTSDV QSAALISIFG SPRYFRDQRV DEWIQTYRDM 850 860 870 880 890 900 LKSWELFSMR ARFDVLRSKL SRTKTGVLTA DIKPRQIYIQ CQNCKQNINT PRTSSPSSAV 910 920 930 940 950 960 STSAGNYKNG EAYRRNNADY KKFNTGSSEA QAADEKPRHK YCCPHCGSSF PRCAICLMPL 970 980 990 1000 1010 1020 GTSNLPFVIN GTQSRDPMQT EDSQDGANRE LVSRKLKLNE WFSFCLSCNH GMHAGHAEEW 1030 FDRHNVCPTP GCTCQCNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:R45.1-R45.13(2003) [PubMed: 12844361] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 942-1001, IDENTIFICATION OF FRAMESHIFT. Strain: ATCC 204511 / S288c / AB972.
[4]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFTS.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Finding functional features in Saccharomyces genomes by phylogenetic footprinting." Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M. Science 301:71-76(2003) [PubMed: 12775844] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFT.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-123; SER-136; SER-476; SER-895; SER-901 AND THR-902, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79489 Genomic DNA. Translation: CAA55991.1. Frameshift. Z35865 Genomic DNA. Translation: CAA84930.1. Frameshift. AY260889 Genomic DNA. Translation: AAP21757.1.
PIR	S45391.
RefSeq	NP_009446.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4369N.
IntAct	P38164. 12 interactions.
STRING	P38164.
Proteomic databases
PeptideAtlas	P38164.
Genome annotation databases
Ensembl	YBL104C; YBL104C; YBL104C; Saccharomyces cerevisiae. [Genome view]
GeneID	852170.
KEGG	sce:YBL104C.
NMPDR	fig|4932.3.peg.131.
Organism-specific databases
CYGD	YBL104c.
SGD	S000000200. YBL104C.
Phylogenomic databases
HOGENOM	P38164.
OMA	FIADNDW
OrthoDB	EOG969SBD
Gene expression databases
ArrayExpress	P38164.
Genevestigator	P38164.
GermOnline	YBL104C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
SMART	SM00320. WD40. 2 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970617.

Entry information

Entry name

YBK4_YEAST

Accession

Primary (citable) accession number: P38164
Secondary accession number(s): Q86ZS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	December 20, 2005
Last modified:	November 24, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)