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P38164

- SEA4_YEAST

UniProt

P38164 - SEA4_YEAST

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Protein

SEH-associated protein 4

Gene

SEA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis.1 Publication

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-28988-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
SEH-associated protein 4
Gene namesi
Name:SEA4
Ordered Locus Names:YBL104C
ORF Names:YBL0808, YBL103C-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBL104c.
SGDiS000000200. SEA4.

Subcellular locationi

GO - Cellular componenti

  1. extrinsic component of fungal-type vacuolar membrane Source: SGD
  2. Seh1-associated complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10381038SEH-associated protein 4PRO_0000051470Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei123 – 1231Phosphoserine1 Publication
Modified residuei136 – 1361Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38164.
PaxDbiP38164.
PeptideAtlasiP38164.

Expressioni

Gene expression databases

GenevestigatoriP38164.

Interactioni

Subunit structurei

Component of the SEA complex composed of at least IML1/SEA1, RTC1/SEA2, MTC5/SEA3, NPR2, NPR3, SEA4, SEC13 and SEH1.1 Publication

Protein-protein interaction databases

BioGridi32599. 64 interactions.
DIPiDIP-4369N.
IntActiP38164. 13 interactions.
MINTiMINT-531382.
STRINGi4932.YBL104C.

Structurei

3D structure databases

ProteinModelPortaliP38164.
SMRiP38164. Positions 306-332, 367-397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati50 – 9041WD 1Add
BLAST
Repeati147 – 18943WD 2Add
BLAST
Repeati235 – 27642WD 3Add
BLAST
Repeati544 – 58744WD 4Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat mio family.Curated
Contains 4 WD repeats.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiNOG134210.
GeneTreeiENSGT00390000015038.
HOGENOMiHOG000246762.
InParanoidiP38164.
OrthoDBiEOG76DV1T.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
3.30.40.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
SMARTiSM00320. WD40. 2 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 3 hits.

Sequencei

Sequence statusi: Complete.

P38164-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLIKKVTHW SYDNLIDYLS VNPTRDEVTH YKVDPENESD ESIIKLHTVK
60 70 80 90 100
DFGSITCLDY SESEIGMIGV GEKNGYLRIF NISGQNSSSP ASHAPVGLNA
110 120 130 140 150
NNETSMTNAS GGKAAQAENI VGSVSNLKDT QGYPVSETNY DIRVRAKKQR
160 170 180 190 200
CINSLGINTN GLIAMGLDRN KHDSSLQIWD MNYHDDSHET INPMFSYCTN
210 220 230 240 250
ESIVSLKFLN DTSVLAASTK FLKEIDVRSP NPIYQHPTRL TYDIKLNPFN
260 270 280 290 300
DWQFSTYGDD GTLAIWDRRK LSDQASLGDL NVASPLLTFE KLVGSGAASR
310 320 330 340 350
KYMNSCFRWS CVRNNEFATL HRGDTIKRWR LGYYCDSNRD IAADDDNEMN
360 370 380 390 400
IENLFVSSVH DTNTMYDRVA TFDYIPRSNN GTSLICMRQS GTIYRMPISE
410 420 430 440 450
VCSKAILNNR NSLLLSNFEN TEIDEIRVNN EHEKSNLENV KTILKNLSFE
460 470 480 490 500
DLDVSEDYFP SGHDEPNNEI EYSELSEEEN EGSNDVLDSK RGFELFWKPE
510 520 530 540 550
KLLEKDISVI MRTRASLGYG LDPMNTVEMI DSSKNLQNNA YIRNTWRWIA
560 570 580 590 600
IAKASVDDGT MVSGDLDLGY EGVIGIWNGI NGISNQDRYR QETILSDKQL
610 620 630 640 650
NKEMEKIIKL RRKNRDRNSP IANAAGSPKY VQRRLCLIIS GWDLSRSDYE
660 670 680 690 700
DKYNIIMKNG HYEKAAAWAV FFGDIPKAVE ILGSAKKERL RLIATAIAGY
710 720 730 740 750
LAYKDLPGNN AWRQQCRKMS SELDDPYLRV IFAFIADNDW WDILYEPAIS
760 770 780 790 800
LRERLGVALR FLNDTDLTTF LDRTSSTVIE NGELEGLILT GITPNGIDLL
810 820 830 840 850
QSYVNKTSDV QSAALISIFG SPRYFRDQRV DEWIQTYRDM LKSWELFSMR
860 870 880 890 900
ARFDVLRSKL SRTKTGVLTA DIKPRQIYIQ CQNCKQNINT PRTSSPSSAV
910 920 930 940 950
STSAGNYKNG EAYRRNNADY KKFNTGSSEA QAADEKPRHK YCCPHCGSSF
960 970 980 990 1000
PRCAICLMPL GTSNLPFVIN GTQSRDPMQT EDSQDGANRE LVSRKLKLNE
1010 1020 1030
WFSFCLSCNH GMHAGHAEEW FDRHNVCPTP GCTCQCNK
Length:1,038
Mass (Da):117,636
Last modified:December 20, 2005 - v2
Checksum:i613630F9F41FF77C
GO

Sequence cautioni

The sequence CAA55991.1 differs from that shown. Reason: Frameshift at positions 2, 7 and 977.
The sequence CAA84930.1 differs from that shown. Reason: Frameshift at positions 2, 7 and 977.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79489 Genomic DNA. Translation: CAA55991.1. Frameshift.
Z35865 Genomic DNA. Translation: CAA84930.1. Frameshift.
AY260889 Genomic DNA. Translation: AAP21757.1.
BK006936 Genomic DNA. Translation: DAA07019.1.
PIRiS45391.
RefSeqiNP_009446.2. NM_001178344.1.

Genome annotation databases

EnsemblFungiiYBL104C; YBL104C; YBL104C.
GeneIDi852170.
KEGGisce:YBL104C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X79489 Genomic DNA. Translation: CAA55991.1 . Frameshift.
Z35865 Genomic DNA. Translation: CAA84930.1 . Frameshift.
AY260889 Genomic DNA. Translation: AAP21757.1 .
BK006936 Genomic DNA. Translation: DAA07019.1 .
PIRi S45391.
RefSeqi NP_009446.2. NM_001178344.1.

3D structure databases

ProteinModelPortali P38164.
SMRi P38164. Positions 306-332, 367-397.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32599. 64 interactions.
DIPi DIP-4369N.
IntActi P38164. 13 interactions.
MINTi MINT-531382.
STRINGi 4932.YBL104C.

Proteomic databases

MaxQBi P38164.
PaxDbi P38164.
PeptideAtlasi P38164.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBL104C ; YBL104C ; YBL104C .
GeneIDi 852170.
KEGGi sce:YBL104C.

Organism-specific databases

CYGDi YBL104c.
SGDi S000000200. SEA4.

Phylogenomic databases

eggNOGi NOG134210.
GeneTreei ENSGT00390000015038.
HOGENOMi HOG000246762.
InParanoidi P38164.
OrthoDBi EOG76DV1T.

Enzyme and pathway databases

BioCyci YEAST:G3O-28988-MONOMER.

Miscellaneous databases

NextBioi 970617.

Gene expression databases

Genevestigatori P38164.

Family and domain databases

Gene3Di 2.130.10.10. 2 hits.
3.30.40.10. 2 hits.
InterProi IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
SMARTi SM00320. WD40. 2 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
    Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
    Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
    Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
    Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 942-1001, IDENTIFICATION OF FRAMESHIFT.
    Strain: ATCC 204511 / S288c / AB972.
  5. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF FRAMESHIFTS.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Finding functional features in Saccharomyces genomes by phylogenetic footprinting."
    Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M.
    Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF FRAMESHIFT.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-123 AND SER-136, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae."
    Dokudovskaya S., Waharte F., Schlessinger A., Pieper U., Devos D.P., Cristea I.M., Williams R., Salamero J., Chait B.T., Sali A., Field M.C., Rout M.P., Dargemont C.
    Mol. Cell. Proteomics 10:M110.006478.1-M110.006478.17(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE SEA COMPLEX, FUNCTION.

Entry informationi

Entry nameiSEA4_YEAST
AccessioniPrimary (citable) accession number: P38164
Secondary accession number(s): D6VPP9, Q86ZS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 184 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3