P38164 (SEA4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SEH-associated protein 4

Gene names

Name:	SEA4
Ordered Locus Names:	YBL104C
ORF Names:	YBL0808, YBL103C-A

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1038 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis. Ref.10
Subunit structure	Component of the SEA complex composed of at least IML1/SEA1, RTC1/SEA2, MTC5/SEA3, NPR2, NPR3, SEA4, SEC13 and SEH1. Ref.10
Subcellular location	Cytoplasm. Vacuole membrane; Peripheral membrane protein Ref.6 Ref.10.
Miscellaneous	Present with 184 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the WD repeat mio family. Contains 4 WD repeats.
Sequence caution	The sequence CAA55991.1 differs from that shown. Reason: Frameshift at positions 2, 7 and 977. The sequence CAA84930.1 differs from that shown. Reason: Frameshift at positions 2, 7 and 977.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Membrane Vacuole
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Seh1-associated complex Inferred from direct assay Ref.10. Source: SGD extrinsic to fungal-type vacuolar membrane Inferred from direct assay Ref.10. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1038	1038	SEH-associated protein 4		PRO_0000051470
Regions
	Repeat	50 – 90	41	WD 1
	Repeat	147 – 189	43	WD 2
	Repeat	235 – 276	42	WD 3
	Repeat	544 – 587	44	WD 4
Amino acid modifications
	Modified residue	123	1	Phosphoserine Ref.9
	Modified residue	136	1	Phosphoserine Ref.9
	Modified residue	476	1	Phosphoserine Ref.9
	Modified residue	895	1	Phosphoserine Ref.9
	Modified residue	901	1	Phosphoserine Ref.9
	Modified residue	902	1	Phosphothreonine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P38164 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 613630F9F41FF77C	FASTA	1,038	117,636
	10 20 30 40 50 60 MGLIKKVTHW SYDNLIDYLS VNPTRDEVTH YKVDPENESD ESIIKLHTVK DFGSITCLDY 70 80 90 100 110 120 SESEIGMIGV GEKNGYLRIF NISGQNSSSP ASHAPVGLNA NNETSMTNAS GGKAAQAENI 130 140 150 160 170 180 VGSVSNLKDT QGYPVSETNY DIRVRAKKQR CINSLGINTN GLIAMGLDRN KHDSSLQIWD 190 200 210 220 230 240 MNYHDDSHET INPMFSYCTN ESIVSLKFLN DTSVLAASTK FLKEIDVRSP NPIYQHPTRL 250 260 270 280 290 300 TYDIKLNPFN DWQFSTYGDD GTLAIWDRRK LSDQASLGDL NVASPLLTFE KLVGSGAASR 310 320 330 340 350 360 KYMNSCFRWS CVRNNEFATL HRGDTIKRWR LGYYCDSNRD IAADDDNEMN IENLFVSSVH 370 380 390 400 410 420 DTNTMYDRVA TFDYIPRSNN GTSLICMRQS GTIYRMPISE VCSKAILNNR NSLLLSNFEN 430 440 450 460 470 480 TEIDEIRVNN EHEKSNLENV KTILKNLSFE DLDVSEDYFP SGHDEPNNEI EYSELSEEEN 490 500 510 520 530 540 EGSNDVLDSK RGFELFWKPE KLLEKDISVI MRTRASLGYG LDPMNTVEMI DSSKNLQNNA 550 560 570 580 590 600 YIRNTWRWIA IAKASVDDGT MVSGDLDLGY EGVIGIWNGI NGISNQDRYR QETILSDKQL 610 620 630 640 650 660 NKEMEKIIKL RRKNRDRNSP IANAAGSPKY VQRRLCLIIS GWDLSRSDYE DKYNIIMKNG 670 680 690 700 710 720 HYEKAAAWAV FFGDIPKAVE ILGSAKKERL RLIATAIAGY LAYKDLPGNN AWRQQCRKMS 730 740 750 760 770 780 SELDDPYLRV IFAFIADNDW WDILYEPAIS LRERLGVALR FLNDTDLTTF LDRTSSTVIE 790 800 810 820 830 840 NGELEGLILT GITPNGIDLL QSYVNKTSDV QSAALISIFG SPRYFRDQRV DEWIQTYRDM 850 860 870 880 890 900 LKSWELFSMR ARFDVLRSKL SRTKTGVLTA DIKPRQIYIQ CQNCKQNINT PRTSSPSSAV 910 920 930 940 950 960 STSAGNYKNG EAYRRNNADY KKFNTGSSEA QAADEKPRHK YCCPHCGSSF PRCAICLMPL 970 980 990 1000 1010 1020 GTSNLPFVIN GTQSRDPMQT EDSQDGANRE LVSRKLKLNE WFSFCLSCNH GMHAGHAEEW 1030 FDRHNVCPTP GCTCQCNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii." Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P. Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 942-1001, IDENTIFICATION OF FRAMESHIFT. Strain: ATCC 204511 / S288c / AB972.
[5]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFTS.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Finding functional features in Saccharomyces genomes by phylogenetic footprinting." Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M. Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF FRAMESHIFT.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-123; SER-136; SER-476; SER-895; SER-901 AND THR-902, MASS SPECTROMETRY.
[10]	"A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae." Dokudovskaya S., Waharte F., Schlessinger A., Pieper U., Devos D.P., Cristea I.M., Williams R., Salamero J., Chait B.T., Sali A., Field M.C., Rout M.P., Dargemont C. Mol. Cell. Proteomics 10:M110.006478.1-M110.006478.17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE SEA COMPLEX, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X79489 Genomic DNA. Translation: CAA55991.1. Frameshift. Z35865 Genomic DNA. Translation: CAA84930.1. Frameshift. AY260889 Genomic DNA. Translation: AAP21757.1. BK006936 Genomic DNA. Translation: DAA07019.1.
PIR	S45391.
RefSeq	NP_009446.2. NM_001178344.1.
3D structure databases
ProteinModelPortal	P38164.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4369N.
IntAct	P38164. 16 interactions.
MINT	MINT-531382.
STRING	4932.YBL104C.
Proteomic databases
PaxDb	P38164.
PeptideAtlas	P38164.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL104C; YBL104C; YBL104C.
GeneID	852170.
KEGG	sce:YBL104C.
Organism-specific databases
CYGD	YBL104c.
SGD	S000000200. SEA4.
Phylogenomic databases
eggNOG	NOG134210.
GeneTree	ENSGT00390000015038.
HOGENOM	HOG000246762.
OMA	FIADNDW.
OrthoDB	EOG4DBXNT.
Gene expression databases
Genevestigator	P38164.
GermOnline	YBL104C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 2 hits. 3.30.40.10. 2 hits.
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR017986. WD40_repeat_dom. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
SMART	SM00320. WD40. 2 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	970617.

Entry information

Entry name

SEA4_YEAST

Accession

Primary (citable) accession number: P38164
Secondary accession number(s): D6VPP9, Q86ZS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program