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P38164 (SEA4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SEH-associated protein 4
Gene names
Name:SEA4
Ordered Locus Names:YBL104C
ORF Names:YBL0808, YBL103C-A
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1038 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis. Ref.11

Subunit structure

Component of the SEA complex composed of at least IML1/SEA1, RTC1/SEA2, MTC5/SEA3, NPR2, NPR3, SEA4, SEC13 and SEH1. Ref.11

Subcellular location

Cytoplasm. Vacuole membrane; Peripheral membrane protein Ref.6 Ref.11.

Miscellaneous

Present with 184 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the WD repeat mio family.

Contains 4 WD repeats.

Sequence caution

The sequence CAA55991.1 differs from that shown. Reason: Frameshift at positions 2, 7 and 977.

The sequence CAA84930.1 differs from that shown. Reason: Frameshift at positions 2, 7 and 977.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10381038SEH-associated protein 4
PRO_0000051470

Regions

Repeat50 – 9041WD 1
Repeat147 – 18943WD 2
Repeat235 – 27642WD 3
Repeat544 – 58744WD 4

Amino acid modifications

Modified residue1231Phosphoserine Ref.9
Modified residue1361Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P38164 [UniParc].

Last modified December 20, 2005. Version 2.
Checksum: 613630F9F41FF77C

FASTA1,038117,636
        10         20         30         40         50         60 
MGLIKKVTHW SYDNLIDYLS VNPTRDEVTH YKVDPENESD ESIIKLHTVK DFGSITCLDY 

        70         80         90        100        110        120 
SESEIGMIGV GEKNGYLRIF NISGQNSSSP ASHAPVGLNA NNETSMTNAS GGKAAQAENI 

       130        140        150        160        170        180 
VGSVSNLKDT QGYPVSETNY DIRVRAKKQR CINSLGINTN GLIAMGLDRN KHDSSLQIWD 

       190        200        210        220        230        240 
MNYHDDSHET INPMFSYCTN ESIVSLKFLN DTSVLAASTK FLKEIDVRSP NPIYQHPTRL 

       250        260        270        280        290        300 
TYDIKLNPFN DWQFSTYGDD GTLAIWDRRK LSDQASLGDL NVASPLLTFE KLVGSGAASR 

       310        320        330        340        350        360 
KYMNSCFRWS CVRNNEFATL HRGDTIKRWR LGYYCDSNRD IAADDDNEMN IENLFVSSVH 

       370        380        390        400        410        420 
DTNTMYDRVA TFDYIPRSNN GTSLICMRQS GTIYRMPISE VCSKAILNNR NSLLLSNFEN 

       430        440        450        460        470        480 
TEIDEIRVNN EHEKSNLENV KTILKNLSFE DLDVSEDYFP SGHDEPNNEI EYSELSEEEN 

       490        500        510        520        530        540 
EGSNDVLDSK RGFELFWKPE KLLEKDISVI MRTRASLGYG LDPMNTVEMI DSSKNLQNNA 

       550        560        570        580        590        600 
YIRNTWRWIA IAKASVDDGT MVSGDLDLGY EGVIGIWNGI NGISNQDRYR QETILSDKQL 

       610        620        630        640        650        660 
NKEMEKIIKL RRKNRDRNSP IANAAGSPKY VQRRLCLIIS GWDLSRSDYE DKYNIIMKNG 

       670        680        690        700        710        720 
HYEKAAAWAV FFGDIPKAVE ILGSAKKERL RLIATAIAGY LAYKDLPGNN AWRQQCRKMS 

       730        740        750        760        770        780 
SELDDPYLRV IFAFIADNDW WDILYEPAIS LRERLGVALR FLNDTDLTTF LDRTSSTVIE 

       790        800        810        820        830        840 
NGELEGLILT GITPNGIDLL QSYVNKTSDV QSAALISIFG SPRYFRDQRV DEWIQTYRDM 

       850        860        870        880        890        900 
LKSWELFSMR ARFDVLRSKL SRTKTGVLTA DIKPRQIYIQ CQNCKQNINT PRTSSPSSAV 

       910        920        930        940        950        960 
STSAGNYKNG EAYRRNNADY KKFNTGSSEA QAADEKPRHK YCCPHCGSSF PRCAICLMPL 

       970        980        990       1000       1010       1020 
GTSNLPFVIN GTQSRDPMQT EDSQDGANRE LVSRKLKLNE WFSFCLSCNH GMHAGHAEEW 

      1030 
FDRHNVCPTP GCTCQCNK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 942-1001, IDENTIFICATION OF FRAMESHIFT.
Strain: ATCC 204511 / S288c / AB972.
[5]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF FRAMESHIFTS.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Finding functional features in Saccharomyces genomes by phylogenetic footprinting."
Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M.
Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF FRAMESHIFT.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-123 AND SER-136, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae."
Dokudovskaya S., Waharte F., Schlessinger A., Pieper U., Devos D.P., Cristea I.M., Williams R., Salamero J., Chait B.T., Sali A., Field M.C., Rout M.P., Dargemont C.
Mol. Cell. Proteomics 10:M110.006478.1-M110.006478.17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE SEA COMPLEX, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79489 Genomic DNA. Translation: CAA55991.1. Frameshift.
Z35865 Genomic DNA. Translation: CAA84930.1. Frameshift.
AY260889 Genomic DNA. Translation: AAP21757.1.
BK006936 Genomic DNA. Translation: DAA07019.1.
PIRS45391.
RefSeqNP_009446.2. NM_001178344.1.

3D structure databases

ProteinModelPortalP38164.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32599. 64 interactions.
DIPDIP-4369N.
IntActP38164. 13 interactions.
MINTMINT-531382.
STRING4932.YBL104C.

Proteomic databases

PaxDbP38164.
PeptideAtlasP38164.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBL104C; YBL104C; YBL104C.
GeneID852170.
KEGGsce:YBL104C.

Organism-specific databases

CYGDYBL104c.
SGDS000000200. SEA4.

Phylogenomic databases

eggNOGNOG134210.
GeneTreeENSGT00390000015038.
HOGENOMHOG000246762.
OrthoDBEOG76DV1T.

Enzyme and pathway databases

BioCycYEAST:G3O-28988-MONOMER.

Gene expression databases

GenevestigatorP38164.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
3.30.40.10. 2 hits.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
SMARTSM00320. WD40. 2 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 3 hits.
ProtoNetSearch...

Other

NextBio970617.

Entry information

Entry nameSEA4_YEAST
AccessionPrimary (citable) accession number: P38164
Secondary accession number(s): D6VPP9, Q86ZS4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 20, 2005
Last modified: March 19, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families