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Protein

SEH-associated protein 4

Gene

SEA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis.1 Publication

Miscellaneous

Present with 184 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • positive regulation of TORC1 signaling Source: SGD
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-28988-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
SEH-associated protein 4
Gene namesi
Name:SEA4
Ordered Locus Names:YBL104C
ORF Names:YBL0808, YBL103C-A
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL104C
SGDiS000000200 SEA4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000514701 – 1038SEH-associated protein 4Add BLAST1038

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei123PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38164
PaxDbiP38164
PRIDEiP38164

PTM databases

iPTMnetiP38164

Interactioni

Subunit structurei

Component of the SEA complex composed of at least IML1/SEA1, RTC1/SEA2, MTC5/SEA3, NPR2, NPR3, SEA4, SEC13 and SEH1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32599, 159 interactors
DIPiDIP-4369N
IntActiP38164, 59 interactors
MINTiP38164
STRINGi4932.YBL104C

Structurei

3D structure databases

ProteinModelPortaliP38164
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati50 – 90WD 1Add BLAST41
Repeati147 – 189WD 2Add BLAST43
Repeati235 – 276WD 3Add BLAST42
Repeati544 – 587WD 4Add BLAST44

Sequence similaritiesi

Belongs to the WD repeat mio family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00390000015038
HOGENOMiHOG000246762
InParanoidiP38164
KOiK20407
OMAiPECRCRC
OrthoDBiEOG092C0FAO

Family and domain databases

InterProiView protein in InterPro
IPR037593 MIOS/Sea4
IPR036322 WD40_repeat_dom_sf
IPR031488 Zn_ribbon_mio
PANTHERiPTHR16453 PTHR16453, 1 hit
PfamiView protein in Pfam
PF17034 zinc_ribbon_16, 1 hit
SUPFAMiSSF50978 SSF50978, 3 hits

Sequencei

Sequence statusi: Complete.

P38164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLIKKVTHW SYDNLIDYLS VNPTRDEVTH YKVDPENESD ESIIKLHTVK
60 70 80 90 100
DFGSITCLDY SESEIGMIGV GEKNGYLRIF NISGQNSSSP ASHAPVGLNA
110 120 130 140 150
NNETSMTNAS GGKAAQAENI VGSVSNLKDT QGYPVSETNY DIRVRAKKQR
160 170 180 190 200
CINSLGINTN GLIAMGLDRN KHDSSLQIWD MNYHDDSHET INPMFSYCTN
210 220 230 240 250
ESIVSLKFLN DTSVLAASTK FLKEIDVRSP NPIYQHPTRL TYDIKLNPFN
260 270 280 290 300
DWQFSTYGDD GTLAIWDRRK LSDQASLGDL NVASPLLTFE KLVGSGAASR
310 320 330 340 350
KYMNSCFRWS CVRNNEFATL HRGDTIKRWR LGYYCDSNRD IAADDDNEMN
360 370 380 390 400
IENLFVSSVH DTNTMYDRVA TFDYIPRSNN GTSLICMRQS GTIYRMPISE
410 420 430 440 450
VCSKAILNNR NSLLLSNFEN TEIDEIRVNN EHEKSNLENV KTILKNLSFE
460 470 480 490 500
DLDVSEDYFP SGHDEPNNEI EYSELSEEEN EGSNDVLDSK RGFELFWKPE
510 520 530 540 550
KLLEKDISVI MRTRASLGYG LDPMNTVEMI DSSKNLQNNA YIRNTWRWIA
560 570 580 590 600
IAKASVDDGT MVSGDLDLGY EGVIGIWNGI NGISNQDRYR QETILSDKQL
610 620 630 640 650
NKEMEKIIKL RRKNRDRNSP IANAAGSPKY VQRRLCLIIS GWDLSRSDYE
660 670 680 690 700
DKYNIIMKNG HYEKAAAWAV FFGDIPKAVE ILGSAKKERL RLIATAIAGY
710 720 730 740 750
LAYKDLPGNN AWRQQCRKMS SELDDPYLRV IFAFIADNDW WDILYEPAIS
760 770 780 790 800
LRERLGVALR FLNDTDLTTF LDRTSSTVIE NGELEGLILT GITPNGIDLL
810 820 830 840 850
QSYVNKTSDV QSAALISIFG SPRYFRDQRV DEWIQTYRDM LKSWELFSMR
860 870 880 890 900
ARFDVLRSKL SRTKTGVLTA DIKPRQIYIQ CQNCKQNINT PRTSSPSSAV
910 920 930 940 950
STSAGNYKNG EAYRRNNADY KKFNTGSSEA QAADEKPRHK YCCPHCGSSF
960 970 980 990 1000
PRCAICLMPL GTSNLPFVIN GTQSRDPMQT EDSQDGANRE LVSRKLKLNE
1010 1020 1030
WFSFCLSCNH GMHAGHAEEW FDRHNVCPTP GCTCQCNK
Length:1,038
Mass (Da):117,636
Last modified:December 20, 2005 - v2
Checksum:i613630F9F41FF77C
GO

Sequence cautioni

The sequence CAA55991 differs from that shown. Reason: Frameshift at positions 2, 7 and 977.Curated
The sequence CAA84930 differs from that shown. Reason: Frameshift at positions 2, 7 and 977.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79489 Genomic DNA Translation: CAA55991.1 Frameshift.
Z35865 Genomic DNA Translation: CAA84930.1 Frameshift.
AY260889 Genomic DNA Translation: AAP21757.1
BK006936 Genomic DNA Translation: DAA07019.1
PIRiS45391
RefSeqiNP_009446.2, NM_001178344.1

Genome annotation databases

EnsemblFungiiYBL104C; YBL104C; YBL104C
GeneIDi852170
KEGGisce:YBL104C

Similar proteinsi

Entry informationi

Entry nameiSEA4_YEAST
AccessioniPrimary (citable) accession number: P38164
Secondary accession number(s): D6VPP9, Q86ZS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health