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P38164

- SEA4_YEAST

UniProt

P38164 - SEA4_YEAST

Protein

SEH-associated protein 4

Gene

SEA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis.1 Publication

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28988-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SEH-associated protein 4
    Gene namesi
    Name:SEA4
    Ordered Locus Names:YBL104C
    ORF Names:YBL0808, YBL103C-A
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL104c.
    SGDiS000000200. SEA4.

    Subcellular locationi

    GO - Cellular componenti

    1. extrinsic component of fungal-type vacuolar membrane Source: SGD
    2. Seh1-associated complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10381038SEH-associated protein 4PRO_0000051470Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei123 – 1231Phosphoserine1 Publication
    Modified residuei136 – 1361Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38164.
    PaxDbiP38164.
    PeptideAtlasiP38164.

    Expressioni

    Gene expression databases

    GenevestigatoriP38164.

    Interactioni

    Subunit structurei

    Component of the SEA complex composed of at least IML1/SEA1, RTC1/SEA2, MTC5/SEA3, NPR2, NPR3, SEA4, SEC13 and SEH1.1 Publication

    Protein-protein interaction databases

    BioGridi32599. 64 interactions.
    DIPiDIP-4369N.
    IntActiP38164. 13 interactions.
    MINTiMINT-531382.
    STRINGi4932.YBL104C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38164.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati50 – 9041WD 1Add
    BLAST
    Repeati147 – 18943WD 2Add
    BLAST
    Repeati235 – 27642WD 3Add
    BLAST
    Repeati544 – 58744WD 4Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat mio family.Curated
    Contains 4 WD repeats.Curated

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiNOG134210.
    GeneTreeiENSGT00390000015038.
    HOGENOMiHOG000246762.
    OrthoDBiEOG76DV1T.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    3.30.40.10. 2 hits.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    SMARTiSM00320. WD40. 2 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    P38164-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLIKKVTHW SYDNLIDYLS VNPTRDEVTH YKVDPENESD ESIIKLHTVK     50
    DFGSITCLDY SESEIGMIGV GEKNGYLRIF NISGQNSSSP ASHAPVGLNA 100
    NNETSMTNAS GGKAAQAENI VGSVSNLKDT QGYPVSETNY DIRVRAKKQR 150
    CINSLGINTN GLIAMGLDRN KHDSSLQIWD MNYHDDSHET INPMFSYCTN 200
    ESIVSLKFLN DTSVLAASTK FLKEIDVRSP NPIYQHPTRL TYDIKLNPFN 250
    DWQFSTYGDD GTLAIWDRRK LSDQASLGDL NVASPLLTFE KLVGSGAASR 300
    KYMNSCFRWS CVRNNEFATL HRGDTIKRWR LGYYCDSNRD IAADDDNEMN 350
    IENLFVSSVH DTNTMYDRVA TFDYIPRSNN GTSLICMRQS GTIYRMPISE 400
    VCSKAILNNR NSLLLSNFEN TEIDEIRVNN EHEKSNLENV KTILKNLSFE 450
    DLDVSEDYFP SGHDEPNNEI EYSELSEEEN EGSNDVLDSK RGFELFWKPE 500
    KLLEKDISVI MRTRASLGYG LDPMNTVEMI DSSKNLQNNA YIRNTWRWIA 550
    IAKASVDDGT MVSGDLDLGY EGVIGIWNGI NGISNQDRYR QETILSDKQL 600
    NKEMEKIIKL RRKNRDRNSP IANAAGSPKY VQRRLCLIIS GWDLSRSDYE 650
    DKYNIIMKNG HYEKAAAWAV FFGDIPKAVE ILGSAKKERL RLIATAIAGY 700
    LAYKDLPGNN AWRQQCRKMS SELDDPYLRV IFAFIADNDW WDILYEPAIS 750
    LRERLGVALR FLNDTDLTTF LDRTSSTVIE NGELEGLILT GITPNGIDLL 800
    QSYVNKTSDV QSAALISIFG SPRYFRDQRV DEWIQTYRDM LKSWELFSMR 850
    ARFDVLRSKL SRTKTGVLTA DIKPRQIYIQ CQNCKQNINT PRTSSPSSAV 900
    STSAGNYKNG EAYRRNNADY KKFNTGSSEA QAADEKPRHK YCCPHCGSSF 950
    PRCAICLMPL GTSNLPFVIN GTQSRDPMQT EDSQDGANRE LVSRKLKLNE 1000
    WFSFCLSCNH GMHAGHAEEW FDRHNVCPTP GCTCQCNK 1038
    Length:1,038
    Mass (Da):117,636
    Last modified:December 20, 2005 - v2
    Checksum:i613630F9F41FF77C
    GO

    Sequence cautioni

    The sequence CAA55991.1 differs from that shown. Reason: Frameshift at positions 2, 7 and 977.
    The sequence CAA84930.1 differs from that shown. Reason: Frameshift at positions 2, 7 and 977.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79489 Genomic DNA. Translation: CAA55991.1. Frameshift.
    Z35865 Genomic DNA. Translation: CAA84930.1. Frameshift.
    AY260889 Genomic DNA. Translation: AAP21757.1.
    BK006936 Genomic DNA. Translation: DAA07019.1.
    PIRiS45391.
    RefSeqiNP_009446.2. NM_001178344.1.

    Genome annotation databases

    EnsemblFungiiYBL104C; YBL104C; YBL104C.
    GeneIDi852170.
    KEGGisce:YBL104C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79489 Genomic DNA. Translation: CAA55991.1 . Frameshift.
    Z35865 Genomic DNA. Translation: CAA84930.1 . Frameshift.
    AY260889 Genomic DNA. Translation: AAP21757.1 .
    BK006936 Genomic DNA. Translation: DAA07019.1 .
    PIRi S45391.
    RefSeqi NP_009446.2. NM_001178344.1.

    3D structure databases

    ProteinModelPortali P38164.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32599. 64 interactions.
    DIPi DIP-4369N.
    IntActi P38164. 13 interactions.
    MINTi MINT-531382.
    STRINGi 4932.YBL104C.

    Proteomic databases

    MaxQBi P38164.
    PaxDbi P38164.
    PeptideAtlasi P38164.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL104C ; YBL104C ; YBL104C .
    GeneIDi 852170.
    KEGGi sce:YBL104C.

    Organism-specific databases

    CYGDi YBL104c.
    SGDi S000000200. SEA4.

    Phylogenomic databases

    eggNOGi NOG134210.
    GeneTreei ENSGT00390000015038.
    HOGENOMi HOG000246762.
    OrthoDBi EOG76DV1T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28988-MONOMER.

    Miscellaneous databases

    NextBioi 970617.

    Gene expression databases

    Genevestigatori P38164.

    Family and domain databases

    Gene3Di 2.130.10.10. 2 hits.
    3.30.40.10. 2 hits.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    SMARTi SM00320. WD40. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
      Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
      Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Reinvestigation of the Saccharomyces cerevisiae genome annotation by comparison to the genome of a related fungus: Ashbya gossypii."
      Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A., Gates K., Gaffney T.D., Philippsen P.
      Genome Biol. 4:R45.1-R45.13(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 942-1001, IDENTIFICATION OF FRAMESHIFT.
      Strain: ATCC 204511 / S288c / AB972.
    5. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF FRAMESHIFTS.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Finding functional features in Saccharomyces genomes by phylogenetic footprinting."
      Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M.
      Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF FRAMESHIFT.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-123 AND SER-136, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae."
      Dokudovskaya S., Waharte F., Schlessinger A., Pieper U., Devos D.P., Cristea I.M., Williams R., Salamero J., Chait B.T., Sali A., Field M.C., Rout M.P., Dargemont C.
      Mol. Cell. Proteomics 10:M110.006478.1-M110.006478.17(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE SEA COMPLEX, FUNCTION.

    Entry informationi

    Entry nameiSEA4_YEAST
    AccessioniPrimary (citable) accession number: P38164
    Secondary accession number(s): D6VPP9, Q86ZS4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 184 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3