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P38158

- MAL32_YEAST

UniProt

P38158 - MAL32_YEAST

Protein

Alpha-glucosidase MAL32

Gene

MAL32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei214 – 2141NucleophileBy similarity
    Active sitei276 – 2761Proton donorBy similarity
    Sitei349 – 3491Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. alpha-1,4-glucosidase activity Source: SGD
    2. cation binding Source: InterPro
    3. glucan 1,4-alpha-maltotriohydrolase activity Source: SGD
    4. maltose alpha-glucosidase activity Source: SGD
    5. sucrose alpha-glucosidase activity Source: SGD

    GO - Biological processi

    1. maltose catabolic process Source: SGD
    2. sucrose catabolic process Source: SGD

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Maltose metabolism

    Enzyme and pathway databases

    BioCyciYEAST:YBR299W-MONOMER.

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-glucosidase MAL32 (EC:3.2.1.20)
    Alternative name(s):
    Maltase
    Gene namesi
    Name:MAL32
    Synonyms:MAL3S
    Ordered Locus Names:YBR299W
    ORF Names:YBR2117
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR299w.
    SGDiS000000503. MAL32.

    Pathology & Biotechi

    Protein family/group databases

    Allergomei8262. Sac c Glucosidase.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 584584Alpha-glucosidase MAL32PRO_0000054329Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP38158.

    Interactioni

    Protein-protein interaction databases

    BioGridi32992. 23 interactions.
    DIPiDIP-6822N.
    IntActiP38158. 5 interactions.
    MINTiMINT-627687.
    STRINGi4932.YBR299W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VADX-ray2.50P438-446[»]
    ProteinModelPortaliP38158.
    SMRiP38158. Positions 4-582.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38158.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Phylogenomic databases

    GeneTreeiENSGT00530000063127.
    HOGENOMiHOG000220641.
    KOiK01187.
    OMAiAIAHIVK.
    OrthoDBiEOG7M3J7X.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38158-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTISDHPETE PKWWKEATIY QIYPASFKDS NNDGWGDLKG ITSKLQYIKD    50
    LGVDAIWVCP FYDSPQQDMG YDISNYEKVW PTYGTNEDCF ELIDKTHKLG 100
    MKFITDLVIN HCSTEHEWFK ESRSSKTNPK RDWFFWRPPK GYDAEGKPIP 150
    PNNWKSFFGG SAWTFDETTN EFYLRLFASR QVDLNWENED CRRAIFESAV 200
    GFWLDHGVDG FRIDTAGLYS KRPGLPDSPI FDKTSKLQHP NWGSHNGPRI 250
    HEYHQELHRF MKNRVKDGRE IMTVGEVAHG SDNALYTSAA RYEVSEVFSF 300
    THVELGTSPF FRYNIVPFTL KQWKEAIASN FLFINGTDSW ATTYIENHDQ 350
    ARSITRFADD SPKYRKISGK LLTLLECSLT GTLYVYQGQE IGQINFKEWP 400
    IEKYEDVDVK NNYEIIKKSF GKNSKEMKDF FKGIALLSRD HSRTPMPWTK 450
    DKPNAGFTGP DVKPWFFLNE SFEQGINVEQ ESRDDDSVLN FWKRALQARK 500
    KYKELMIYGY DFQFIDLDSD QIFSFTKEYE DKTLFAALNF SGEEIEFSLP 550
    REGASLSFIL GNYDDTDVSS RVLKPWEGRI YLVK 584
    Length:584
    Mass (Da):68,142
    Last modified:October 1, 1994 - v1
    Checksum:i4C461F5F40B464C3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36168 Genomic DNA. Translation: CAA85264.1.
    BK006936 Genomic DNA. Translation: DAA07415.1.
    PIRiS46183.
    RefSeqiNP_009858.3. NM_001178647.3.

    Genome annotation databases

    EnsemblFungiiYBR299W; YBR299W; YBR299W.
    GeneIDi852602.
    KEGGisce:YBR299W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36168 Genomic DNA. Translation: CAA85264.1 .
    BK006936 Genomic DNA. Translation: DAA07415.1 .
    PIRi S46183.
    RefSeqi NP_009858.3. NM_001178647.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VAD X-ray 2.50 P 438-446 [» ]
    ProteinModelPortali P38158.
    SMRi P38158. Positions 4-582.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32992. 23 interactions.
    DIPi DIP-6822N.
    IntActi P38158. 5 interactions.
    MINTi MINT-627687.
    STRINGi 4932.YBR299W.

    Chemistry

    BindingDBi P38158.

    Protein family/group databases

    Allergomei 8262. Sac c Glucosidase.
    CAZyi GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR299W ; YBR299W ; YBR299W .
    GeneIDi 852602.
    KEGGi sce:YBR299W.

    Organism-specific databases

    CYGDi YBR299w.
    SGDi S000000503. MAL32.

    Phylogenomic databases

    GeneTreei ENSGT00530000063127.
    HOGENOMi HOG000220641.
    KOi K01187.
    OMAi AIAHIVK.
    OrthoDBi EOG7M3J7X.

    Enzyme and pathway databases

    BioCyci YEAST:YBR299W-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P38158.
    NextBioi 971784.

    Gene expression databases

    Genevestigatori P38158.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    [Graphical view ]
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of a 9.8 kb segment of yeast chromosome II including the three genes of the MAL3 locus and three unidentified open reading frames."
      Feuermann M., Charbonnel L., De Montigny J., Bloch J.C., Potier S., Souciet J.-L.
      Yeast 11:667-672(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMAL32_YEAST
    AccessioniPrimary (citable) accession number: P38158
    Secondary accession number(s): D6VQU5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4030 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3