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P38158

- MAL32_YEAST

UniProt

P38158 - MAL32_YEAST

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Protein

Alpha-glucosidase MAL32

Gene
MAL32, MAL3S, YBR299W, YBR2117
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei214 – 2141Nucleophile By similarity
Active sitei276 – 2761Proton donor By similarity
Sitei349 – 3491Transition state stabilizer By similarity

GO - Molecular functioni

  1. alpha-glucosidase activity Source: SGD
  2. cation binding Source: InterPro
  3. glucan 1,4-alpha-maltotriohydrolase activity Source: SGD
  4. maltose alpha-glucosidase activity Source: SGD
  5. sucrose alpha-glucosidase activity Source: SGD

GO - Biological processi

  1. maltose catabolic process Source: SGD
  2. sucrose catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Maltose metabolism

Enzyme and pathway databases

BioCyciYEAST:YBR299W-MONOMER.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-glucosidase MAL32 (EC:3.2.1.20)
Alternative name(s):
Maltase
Gene namesi
Name:MAL32
Synonyms:MAL3S
Ordered Locus Names:YBR299W
ORF Names:YBR2117
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR299w.
SGDiS000000503. MAL32.

Pathology & Biotechi

Protein family/group databases

Allergomei8262. Sac c Glucosidase.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 584584Alpha-glucosidase MAL32PRO_0000054329Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP38158.

Interactioni

Protein-protein interaction databases

BioGridi32992. 23 interactions.
DIPiDIP-6822N.
IntActiP38158. 5 interactions.
MINTiMINT-627687.
STRINGi4932.YBR299W.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VADX-ray2.50P438-446[»]
ProteinModelPortaliP38158.
SMRiP38158. Positions 4-582.

Miscellaneous databases

EvolutionaryTraceiP38158.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000063127.
HOGENOMiHOG000220641.
KOiK01187.
OMAiAIAHIVK.
OrthoDBiEOG7M3J7X.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P38158-1 [UniParc]FASTAAdd to Basket

« Hide

MTISDHPETE PKWWKEATIY QIYPASFKDS NNDGWGDLKG ITSKLQYIKD    50
LGVDAIWVCP FYDSPQQDMG YDISNYEKVW PTYGTNEDCF ELIDKTHKLG 100
MKFITDLVIN HCSTEHEWFK ESRSSKTNPK RDWFFWRPPK GYDAEGKPIP 150
PNNWKSFFGG SAWTFDETTN EFYLRLFASR QVDLNWENED CRRAIFESAV 200
GFWLDHGVDG FRIDTAGLYS KRPGLPDSPI FDKTSKLQHP NWGSHNGPRI 250
HEYHQELHRF MKNRVKDGRE IMTVGEVAHG SDNALYTSAA RYEVSEVFSF 300
THVELGTSPF FRYNIVPFTL KQWKEAIASN FLFINGTDSW ATTYIENHDQ 350
ARSITRFADD SPKYRKISGK LLTLLECSLT GTLYVYQGQE IGQINFKEWP 400
IEKYEDVDVK NNYEIIKKSF GKNSKEMKDF FKGIALLSRD HSRTPMPWTK 450
DKPNAGFTGP DVKPWFFLNE SFEQGINVEQ ESRDDDSVLN FWKRALQARK 500
KYKELMIYGY DFQFIDLDSD QIFSFTKEYE DKTLFAALNF SGEEIEFSLP 550
REGASLSFIL GNYDDTDVSS RVLKPWEGRI YLVK 584
Length:584
Mass (Da):68,142
Last modified:October 1, 1994 - v1
Checksum:i4C461F5F40B464C3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z36168 Genomic DNA. Translation: CAA85264.1.
BK006936 Genomic DNA. Translation: DAA07415.1.
PIRiS46183.
RefSeqiNP_009858.3. NM_001178647.3.

Genome annotation databases

EnsemblFungiiYBR299W; YBR299W; YBR299W.
GeneIDi852602.
KEGGisce:YBR299W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z36168 Genomic DNA. Translation: CAA85264.1 .
BK006936 Genomic DNA. Translation: DAA07415.1 .
PIRi S46183.
RefSeqi NP_009858.3. NM_001178647.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VAD X-ray 2.50 P 438-446 [» ]
ProteinModelPortali P38158.
SMRi P38158. Positions 4-582.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32992. 23 interactions.
DIPi DIP-6822N.
IntActi P38158. 5 interactions.
MINTi MINT-627687.
STRINGi 4932.YBR299W.

Chemistry

BindingDBi P38158.

Protein family/group databases

Allergomei 8262. Sac c Glucosidase.
CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR299W ; YBR299W ; YBR299W .
GeneIDi 852602.
KEGGi sce:YBR299W.

Organism-specific databases

CYGDi YBR299w.
SGDi S000000503. MAL32.

Phylogenomic databases

GeneTreei ENSGT00530000063127.
HOGENOMi HOG000220641.
KOi K01187.
OMAi AIAHIVK.
OrthoDBi EOG7M3J7X.

Enzyme and pathway databases

BioCyci YEAST:YBR299W-MONOMER.

Miscellaneous databases

EvolutionaryTracei P38158.
NextBioi 971784.

Gene expression databases

Genevestigatori P38158.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
[Graphical view ]
SMARTi SM00642. Aamy. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of a 9.8 kb segment of yeast chromosome II including the three genes of the MAL3 locus and three unidentified open reading frames."
    Feuermann M., Charbonnel L., De Montigny J., Bloch J.C., Potier S., Souciet J.-L.
    Yeast 11:667-672(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMAL32_YEAST
AccessioniPrimary (citable) accession number: P38158
Secondary accession number(s): D6VQU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 14, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4030 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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