P38158 (MAL32_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-glucosidase MAL32
EC=3.2.1.20

Alternative name(s):
Maltase

Gene names

Name:	MAL32
Synonyms:	MAL3S
Ordered Locus Names:	YBR299W
ORF Names:	YBR2117

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Miscellaneous	Present with 4030 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Maltose metabolism
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	maltose catabolic process Inferred from genetic interaction. Source: SGD sucrose catabolic process Inferred from genetic interaction. Source: SGD
Cellular component	plasma membrane enriched fraction Inferred from direct assay. Source: SGD
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro maltose alpha-glucosidase activity Inferred from direct assay. Source: SGD sucrose alpha-glucosidase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 584	584	Alpha-glucosidase MAL32		PRO_0000054329
Sites
	Active site	214	1	Nucleophile By similarity
	Active site	276	1	Proton donor By similarity
	Site	349	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38158 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 4C461F5F40B464C3	FASTA	584	68,142
	10 20 30 40 50 60 MTISDHPETE PKWWKEATIY QIYPASFKDS NNDGWGDLKG ITSKLQYIKD LGVDAIWVCP 70 80 90 100 110 120 FYDSPQQDMG YDISNYEKVW PTYGTNEDCF ELIDKTHKLG MKFITDLVIN HCSTEHEWFK 130 140 150 160 170 180 ESRSSKTNPK RDWFFWRPPK GYDAEGKPIP PNNWKSFFGG SAWTFDETTN EFYLRLFASR 190 200 210 220 230 240 QVDLNWENED CRRAIFESAV GFWLDHGVDG FRIDTAGLYS KRPGLPDSPI FDKTSKLQHP 250 260 270 280 290 300 NWGSHNGPRI HEYHQELHRF MKNRVKDGRE IMTVGEVAHG SDNALYTSAA RYEVSEVFSF 310 320 330 340 350 360 THVELGTSPF FRYNIVPFTL KQWKEAIASN FLFINGTDSW ATTYIENHDQ ARSITRFADD 370 380 390 400 410 420 SPKYRKISGK LLTLLECSLT GTLYVYQGQE IGQINFKEWP IEKYEDVDVK NNYEIIKKSF 430 440 450 460 470 480 GKNSKEMKDF FKGIALLSRD HSRTPMPWTK DKPNAGFTGP DVKPWFFLNE SFEQGINVEQ 490 500 510 520 530 540 ESRDDDSVLN FWKRALQARK KYKELMIYGY DFQFIDLDSD QIFSFTKEYE DKTLFAALNF 550 560 570 580 SGEEIEFSLP REGASLSFIL GNYDDTDVSS RVLKPWEGRI YLVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 9.8 kb segment of yeast chromosome II including the three genes of the MAL3 locus and three unidentified open reading frames." Feuermann M., Charbonnel L., De Montigny J., Bloch J.C., Potier S., Souciet J.-L. Yeast 11:667-672(1995) [PubMed: 7483839] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z36168 Genomic DNA. Translation: CAA85264.1.
BK006936 Genomic DNA. Translation: DAA07415.1.

PIR

S46183.

RefSeq

NP_009858.1. NM_001178647.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VAD	X-ray	2.50	P	438-446	[»]

ProteinModelPortal

P38158.

SMR

P38158. Positions 4-582.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6822N.

IntAct

P38158. 5 interactions.

MINT

MINT-627687.

STRING

P38158.

Protein family/group databases

Allergome

8262. Sac c Glucosidase.

CAZy

GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YBR299W; YBR299W; YBR299W.

GeneID

852602.

KEGG

sce:YBR299W.

Organism-specific databases

CYGD

YBR299w.

SGD

S000000503. MAL32.

Phylogenomic databases

GeneTree

EFGT00050000000929.

HOGENOM

HBG669028.

OMA

WFLDSRS.

OrthoDB

EOG4K0TWQ.

Gene expression databases

Genevestigator

P38158.

GermOnline

YBR299W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR015902. Alpha_amylase.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Gene3D

G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.

KO

K01187.

PANTHER

PTHR10357. Alpha_amylase. 1 hit.

Pfam

PF00128. Alpha-amylase. 1 hit.
[Graphical view]

SMART

SM00642. Aamy. 1 hit.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

ProtoNet

Search...

Other

BindingDB

P38158.

NextBio

971784.

Entry information

Entry name

MAL32_YEAST

Accession

Primary (citable) accession number: P38158
Secondary accession number(s): D6VQU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program