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Protein

AP-3 complex subunit mu

Gene

APM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. Required for the transport via the ALP pathway, which directs the transport of the cargo proteins PHO8 and VAM3 to the vacuole.3 Publications

GO - Biological processi

  • Golgi to vacuole transport Source: SGD
  • protein targeting to vacuole Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29207-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit mu
Alternative name(s):
AP-3 adaptor complex mu3A subunit
Adaptor-related protein complex 3 subunit mu
Mu-adaptin 3A
Mu3-adaptin
Gene namesi
Name:APM3
Synonyms:YKS6
Ordered Locus Names:YBR288C
ORF Names:YBR2035
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR288C.
SGDiS000000492. APM3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483AP-3 complex subunit muPRO_0000193794Add
BLAST

Proteomic databases

MaxQBiP38153.
PeptideAtlasiP38153.

PTM databases

iPTMnetiP38153.

Interactioni

Subunit structurei

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of 2 large adaptins (APL5 and APL6), a medium adaptin (APM3) and a small adaptin (APS3).

Binary interactionsi

WithEntry#Exp.IntActNotes
APL5Q089513EBI-2710,EBI-29702
APL6P466825EBI-2710,EBI-2213

Protein-protein interaction databases

BioGridi32982. 116 interactions.
DIPiDIP-4965N.
IntActiP38153. 5 interactions.
MINTiMINT-530418.

Structurei

3D structure databases

ProteinModelPortaliP38153.
SMRiP38153. Positions 66-482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini211 – 482272MHDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000033979.
InParanoidiP38153.
OMAiFDSAFYS.
OrthoDBiEOG7673N3.

Family and domain databases

InterProiIPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
SUPFAMiSSF49447. SSF49447. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYLSFYITDT KNKLIFQYLL GATAPSFKHL WTRVQSTCPQ LLEDSSSDDY
60 70 80 90 100
LDHSMVGRDL EVYKYFSVIN KLNYWCLAST SKSKGPLDCF TFLETIDRIL
110 120 130 140 150
LEYFDKDKLS IKKIVNNYDR ISLIFNCCVE AGEPNVSDML YVNKIKEAVP
160 170 180 190 200
ERSDLSKFIS STAHNLQQAV QLPQQRQQQL QQNQISRGSN SLIENEEIVP
210 220 230 240 250
WRTSRASKHE NNELYVDLLE TFHVVFEKKK SHLRLLTGSI HGIVDVRSYL
260 270 280 290 300
NDNPLVAVKL NTMGNDIGIP SLHDCVEIND GVFSPSNITF IPPDGKFRLL
310 320 330 340 350
EYSVDLSSQV KQSGVRMNSI GLMSLHFQNG LGKDSDEFEL SLNIENFKKV
360 370 380 390 400
SQVDDLKIDL QFNVENADPN EIAYKIKILR NTHGRFENSI IMGQGQWIFD
410 420 430 440 450
KSTATGTVPV LRGCIEYENT GPNFTKKVDL QTVSLEYSYI GQSASGIYVE
460 470 480
AIDIVSGLTI GKNTKLYKGA KYKTQTGNFQ VRL
Length:483
Mass (Da):54,837
Last modified:July 27, 2011 - v2
Checksum:iAAD5A7EA305C63CF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 362QS → RT in CAA53651 (PubMed:8091861).Curated
Sequence conflicti35 – 362QS → RT in CAA85253 (PubMed:7813418).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76053 Genomic DNA. Translation: CAA53651.1.
Z36157 Genomic DNA. Translation: CAA85253.1.
BK006936 Genomic DNA. Translation: DAA07403.2.
PIRiS44550.
RefSeqiNP_009847.4. NM_001178636.4.

Genome annotation databases

EnsemblFungiiYBR288C; YBR288C; YBR288C.
GeneIDi852591.
KEGGisce:YBR288C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76053 Genomic DNA. Translation: CAA53651.1.
Z36157 Genomic DNA. Translation: CAA85253.1.
BK006936 Genomic DNA. Translation: DAA07403.2.
PIRiS44550.
RefSeqiNP_009847.4. NM_001178636.4.

3D structure databases

ProteinModelPortaliP38153.
SMRiP38153. Positions 66-482.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32982. 116 interactions.
DIPiDIP-4965N.
IntActiP38153. 5 interactions.
MINTiMINT-530418.

PTM databases

iPTMnetiP38153.

Proteomic databases

MaxQBiP38153.
PeptideAtlasiP38153.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR288C; YBR288C; YBR288C.
GeneIDi852591.
KEGGisce:YBR288C.

Organism-specific databases

EuPathDBiFungiDB:YBR288C.
SGDiS000000492. APM3.

Phylogenomic databases

HOGENOMiHOG000033979.
InParanoidiP38153.
OMAiFDSAFYS.
OrthoDBiEOG7673N3.

Enzyme and pathway databases

BioCyciYEAST:G3O-29207-MONOMER.

Miscellaneous databases

NextBioi971754.
PROiP38153.

Family and domain databases

InterProiIPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
SUPFAMiSSF49447. SSF49447. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
    Holmstroem K., Brandt T., Kallesoe T.
    Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 35-36.
    Strain: ATCC 204508 / S288c.
  4. "The AP-3 adaptor complex is essential for cargo-selective transport to the yeast vacuole."
    Cowles C.R., Odorizzi G., Payne G.S., Emr S.D.
    Cell 91:109-118(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
  5. "Suppressors of YCK-encoded yeast casein kinase 1 deficiency define the four subunits of a novel clathrin AP-like complex."
    Panek H.R., Stepp J.D., Engle H.M., Marks K.M., Tan P.K., Lemmon S.K., Robinson L.C.
    EMBO J. 16:4194-4204(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF THE AP-3 COMPLEX, FUNCTION OF THE AP-3 COMPLEX.
  6. "Formation of AP-3 transport intermediates requires Vps41 function."
    Rehling P., Darsow T., Katzmann D.J., Emr S.D.
    Nat. Cell Biol. 1:346-353(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION OF THE AP-3 COMPLEX.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAP3M_YEAST
AccessioniPrimary (citable) accession number: P38153
Secondary accession number(s): D6VQT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3410 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.