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P38149 (DUG2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable di- and tripeptidase DUG2

EC=3.4.-.-
Alternative name(s):
Deficient in utilization of glutathione protein 2
GSH degradosomal complex subunit DUG2
Gene names
Name:DUG2
Ordered Locus Names:YBR281C
ORF Names:YBR2018
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length878 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the GSH degradosomal complex involved in the degradation of glutathione (GSH) and other peptides containing a gamma-glu-X bond. Ref.6

Cofactor

Binds 2 zinc ions per subunit By similarity.

Subunit structure

Component of the GSH degradosomal complex composed of at least DUG1, DUG2 and DUG3. Ref.6

Subcellular location

Cytoplasm. Nucleus Ref.4 Ref.6.

Miscellaneous

Present with 1940 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the peptidase M20A family.

Contains 6 WD repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 878878Probable di- and tripeptidase DUG2
PRO_0000051469

Regions

Repeat18 – 5740WD 1
Repeat68 – 10740WD 2
Repeat235 – 27440WD 3
Repeat282 – 32241WD 4
Repeat362 – 40544WD 5
Repeat608 – 65144WD 6

Sites

Active site5221 By similarity
Active site5861Proton acceptor By similarity
Metal binding5201Zinc 2 By similarity
Metal binding5531Zinc 1 By similarity
Metal binding5531Zinc 2 By similarity
Metal binding5871Zinc 1 By similarity
Metal binding8531Zinc 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
P38149 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: A24C10BB4E94C344

FASTA87898,071
        10         20         30         40         50         60 
MYDSRGVALH SELIHRWNHA FSILSIVAFP KKRLLFAGSQ DSKILVFDLP TYNLIHTIRL 

        70         80         90        100        110        120 
GESQEETHTR SSVLCLTGSE DENFLFSGGA DSLVRIWSIG EKTIRDDFLP VTEIATVYSV 

       130        140        150        160        170        180 
TDIGDIFSLA YLDSLETIVF GCQNASLLYV ENLIQKIEKK SSDGVENINK LPHRRYDKFF 

       190        200        210        220        230        240 
DSLGPTGYSS NSLSQTSLTS LQENCGAAII EVPSENIIKY AHYGFIYSIN KLCPRFNQLL 

       250        260        270        280        290        300 
EKSSRTSGAE HIISSAGDGI SKLWEFSKDK GQNTVKISLI NDKIDNEDSV ISQTIEFPFL 

       310        320        330        340        350        360 
YCGLTDGIIK IWDLNTQQII STLKTKHESD VISISVYMDH VFAIDESGIT HFYQNQVNHW 

       370        380        390        400        410        420 
NPQQGKILSS EIFSKSNAGS VSLLTGGSDG SLTLWDITSL LSAVPLSSNS PINASSTLQT 

       430        440        450        460        470        480 
TNLWAAYQSA SLNNEEMLNT LRELISFQTV SQSKDTTNTL SLRRCAIYLQ QLFLKFGATN 

       490        500        510        520        530        540 
SQLFPLPDGG NPVVFAYFQG NGKVSQVKGA KKKRILWYGH YDVISSGNTF NWNTDPFTLT 

       550        560        570        580        590        600 
CENGYLKGRG VSDNKGPLVS AIHSVAYLFQ QGELVNDVVF LVEGSEEIGS ASLKQVCEKY 

       610        620        630        640        650        660 
HDIIGKDIDW ILLSNSTWVD QEHPCLNYGL RGVINAQIKV WSDKPDGHSG LNGGVYDEPM 

       670        680        690        700        710        720 
VNLVKIVSKL QNEQNEIMIP NFYSPLKDLT EEEYQRFQKI TELANIDENT TVQDLITNWT 

       730        740        750        760        770        780 
KPSLSMTTVK FSGPGNITVI PKSVTMGISI RLVPEQSVEQ VKRDLKAYLE ESFKQLKSQN 

       790        800        810        820        830        840 
HLEIKVLNEA EGWLGDPTNH AYQILKDEIT TAWDVEPLLV REGGSISCLR MLERIFDAPA 

       850        860        870 
VQIPCGQSTD NGHLANENLR IKNWSNLTEI LSKVFNRL 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
Holmstroem K., Brandt T., Kallesoe T.
Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"The alternative pathway of glutathione degradation is mediated by a novel protein complex involving three new genes in Saccharomyces cerevisiae."
Ganguli D., Kumar C., Bachhawat A.K.
Genetics 175:1137-1151(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE GSH DEGRADOSOMAL COMPLEX, SUBCELLULAR LOCATION.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X76053 Genomic DNA. Translation: CAA53644.1.
Z36150 Genomic DNA. Translation: CAA85245.1.
BK006936 Genomic DNA. Translation: DAA07396.1.
PIRS44543.
RefSeqNP_009840.3. NM_001178629.3.

3D structure databases

ProteinModelPortalP38149.
SMRP38149. Positions 423-875.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32975. 18 interactions.
DIPDIP-1612N.
IntActP38149. 4 interactions.
MINTMINT-399156.
STRING4932.YBR281C.

Proteomic databases

PaxDbP38149.
PeptideAtlasP38149.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR281C; YBR281C; YBR281C.
GeneID852584.
KEGGsce:YBR281C.

Organism-specific databases

CYGDYBR281c.
SGDS000000485. DUG2.

Phylogenomic databases

eggNOGCOG0624.
HOGENOMHOG000183136.
KOK14262.
OMAPAAHLPC.
OrthoDBEOG7NW6JF.

Enzyme and pathway databases

BioCycYEAST:G3O-29201-MONOMER.

Gene expression databases

GenevestigatorP38149.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
3.30.70.360. 1 hit.
InterProIPR020472. G-protein_beta_WD-40_rep.
IPR017149. GSH_degradosome_DUG2.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
PIRSFPIRSF037237. Peptidase_WD_repeats_DUG2. 1 hit.
PRINTSPR00320. GPROTEINBRPT.
SMARTSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 2 hits.
SSF55031. SSF55031. 1 hit.
PROSITEPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971733.

Entry information

Entry nameDUG2_YEAST
AccessionPrimary (citable) accession number: P38149
Secondary accession number(s): D6VQS6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries