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P38149

- DUG2_YEAST

UniProt

P38149 - DUG2_YEAST

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Protein

Probable di- and tripeptidase DUG2

Gene

DUG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the GSH degradosomal complex involved in the degradation of glutathione (GSH) and other peptides containing a gamma-glu-X bond.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn(2+) ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi520 – 5201Zinc 2By similarity
Active sitei522 – 5221By similarity
Metal bindingi553 – 5531Zinc 1By similarity
Metal bindingi553 – 5531Zinc 2By similarity
Active sitei586 – 5861Proton acceptorBy similarity
Metal bindingi587 – 5871Zinc 1By similarity
Metal bindingi853 – 8531Zinc 1By similarity

GO - Molecular functioni

  1. dipeptidase activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. metallopeptidase activity Source: UniProtKB-KW
  4. omega peptidase activity Source: SGD
  5. peptidase activity Source: SGD

GO - Biological processi

  1. glutathione catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Dipeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29201-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable di- and tripeptidase DUG2 (EC:3.4.-.-)
Alternative name(s):
Deficient in utilization of glutathione protein 2
GSH degradosomal complex subunit DUG2
Gene namesi
Name:DUG2
Ordered Locus Names:YBR281C
ORF Names:YBR2018
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR281c.
SGDiS000000485. DUG2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 878878Probable di- and tripeptidase DUG2PRO_0000051469Add
BLAST

Proteomic databases

MaxQBiP38149.
PaxDbiP38149.
PeptideAtlasiP38149.

Expressioni

Gene expression databases

GenevestigatoriP38149.

Interactioni

Subunit structurei

Component of the GSH degradosomal complex composed of at least DUG1, DUG2 and DUG3.1 Publication

Protein-protein interaction databases

BioGridi32975. 21 interactions.
DIPiDIP-1612N.
IntActiP38149. 4 interactions.
MINTiMINT-399156.
STRINGi4932.YBR281C.

Structurei

3D structure databases

ProteinModelPortaliP38149.
SMRiP38149. Positions 20-102, 237-270, 423-875.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati18 – 5740WD 1Add
BLAST
Repeati68 – 10740WD 2Add
BLAST
Repeati235 – 27440WD 3Add
BLAST
Repeati282 – 32241WD 4Add
BLAST
Repeati362 – 40544WD 5Add
BLAST
Repeati608 – 65144WD 6Add
BLAST

Sequence similaritiesi

Belongs to the peptidase M20A family.Curated
Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG0624.
HOGENOMiHOG000183136.
InParanoidiP38149.
KOiK14262.
OMAiPAAHLPC.
OrthoDBiEOG7NW6JF.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
3.30.70.360. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR017149. GSH_degradosome_DUG2.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view]
PIRSFiPIRSF037237. Peptidase_WD_repeats_DUG2. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
SSF55031. SSF55031. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38149-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYDSRGVALH SELIHRWNHA FSILSIVAFP KKRLLFAGSQ DSKILVFDLP
60 70 80 90 100
TYNLIHTIRL GESQEETHTR SSVLCLTGSE DENFLFSGGA DSLVRIWSIG
110 120 130 140 150
EKTIRDDFLP VTEIATVYSV TDIGDIFSLA YLDSLETIVF GCQNASLLYV
160 170 180 190 200
ENLIQKIEKK SSDGVENINK LPHRRYDKFF DSLGPTGYSS NSLSQTSLTS
210 220 230 240 250
LQENCGAAII EVPSENIIKY AHYGFIYSIN KLCPRFNQLL EKSSRTSGAE
260 270 280 290 300
HIISSAGDGI SKLWEFSKDK GQNTVKISLI NDKIDNEDSV ISQTIEFPFL
310 320 330 340 350
YCGLTDGIIK IWDLNTQQII STLKTKHESD VISISVYMDH VFAIDESGIT
360 370 380 390 400
HFYQNQVNHW NPQQGKILSS EIFSKSNAGS VSLLTGGSDG SLTLWDITSL
410 420 430 440 450
LSAVPLSSNS PINASSTLQT TNLWAAYQSA SLNNEEMLNT LRELISFQTV
460 470 480 490 500
SQSKDTTNTL SLRRCAIYLQ QLFLKFGATN SQLFPLPDGG NPVVFAYFQG
510 520 530 540 550
NGKVSQVKGA KKKRILWYGH YDVISSGNTF NWNTDPFTLT CENGYLKGRG
560 570 580 590 600
VSDNKGPLVS AIHSVAYLFQ QGELVNDVVF LVEGSEEIGS ASLKQVCEKY
610 620 630 640 650
HDIIGKDIDW ILLSNSTWVD QEHPCLNYGL RGVINAQIKV WSDKPDGHSG
660 670 680 690 700
LNGGVYDEPM VNLVKIVSKL QNEQNEIMIP NFYSPLKDLT EEEYQRFQKI
710 720 730 740 750
TELANIDENT TVQDLITNWT KPSLSMTTVK FSGPGNITVI PKSVTMGISI
760 770 780 790 800
RLVPEQSVEQ VKRDLKAYLE ESFKQLKSQN HLEIKVLNEA EGWLGDPTNH
810 820 830 840 850
AYQILKDEIT TAWDVEPLLV REGGSISCLR MLERIFDAPA VQIPCGQSTD
860 870
NGHLANENLR IKNWSNLTEI LSKVFNRL
Length:878
Mass (Da):98,071
Last modified:October 1, 1994 - v1
Checksum:iA24C10BB4E94C344
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76053 Genomic DNA. Translation: CAA53644.1.
Z36150 Genomic DNA. Translation: CAA85245.1.
BK006936 Genomic DNA. Translation: DAA07396.1.
PIRiS44543.
RefSeqiNP_009840.3. NM_001178629.3.

Genome annotation databases

EnsemblFungiiYBR281C; YBR281C; YBR281C.
GeneIDi852584.
KEGGisce:YBR281C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76053 Genomic DNA. Translation: CAA53644.1 .
Z36150 Genomic DNA. Translation: CAA85245.1 .
BK006936 Genomic DNA. Translation: DAA07396.1 .
PIRi S44543.
RefSeqi NP_009840.3. NM_001178629.3.

3D structure databases

ProteinModelPortali P38149.
SMRi P38149. Positions 20-102, 237-270, 423-875.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32975. 21 interactions.
DIPi DIP-1612N.
IntActi P38149. 4 interactions.
MINTi MINT-399156.
STRINGi 4932.YBR281C.

Proteomic databases

MaxQBi P38149.
PaxDbi P38149.
PeptideAtlasi P38149.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR281C ; YBR281C ; YBR281C .
GeneIDi 852584.
KEGGi sce:YBR281C.

Organism-specific databases

CYGDi YBR281c.
SGDi S000000485. DUG2.

Phylogenomic databases

eggNOGi COG0624.
HOGENOMi HOG000183136.
InParanoidi P38149.
KOi K14262.
OMAi PAAHLPC.
OrthoDBi EOG7NW6JF.

Enzyme and pathway databases

BioCyci YEAST:G3O-29201-MONOMER.

Miscellaneous databases

NextBioi 971733.

Gene expression databases

Genevestigatori P38149.

Family and domain databases

Gene3Di 2.130.10.10. 2 hits.
3.30.70.360. 1 hit.
InterProi IPR020472. G-protein_beta_WD-40_rep.
IPR017149. GSH_degradosome_DUG2.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
PF00400. WD40. 1 hit.
[Graphical view ]
PIRSFi PIRSF037237. Peptidase_WD_repeats_DUG2. 1 hit.
PRINTSi PR00320. GPROTEINBRPT.
SMARTi SM00320. WD40. 4 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 2 hits.
SSF55031. SSF55031. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
    Holmstroem K., Brandt T., Kallesoe T.
    Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The alternative pathway of glutathione degradation is mediated by a novel protein complex involving three new genes in Saccharomyces cerevisiae."
    Ganguli D., Kumar C., Bachhawat A.K.
    Genetics 175:1137-1151(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE GSH DEGRADOSOMAL COMPLEX, SUBCELLULAR LOCATION.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDUG2_YEAST
AccessioniPrimary (citable) accession number: P38149
Secondary accession number(s): D6VQS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 26, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1940 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3