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P38149

- DUG2_YEAST

UniProt

P38149 - DUG2_YEAST

Protein

Probable di- and tripeptidase DUG2

Gene

DUG2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Component of the GSH degradosomal complex involved in the degradation of glutathione (GSH) and other peptides containing a gamma-glu-X bond.1 Publication

    Cofactori

    Binds 2 zinc ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi520 – 5201Zinc 2By similarity
    Active sitei522 – 5221By similarity
    Metal bindingi553 – 5531Zinc 1By similarity
    Metal bindingi553 – 5531Zinc 2By similarity
    Active sitei586 – 5861Proton acceptorBy similarity
    Metal bindingi587 – 5871Zinc 1By similarity
    Metal bindingi853 – 8531Zinc 1By similarity

    GO - Molecular functioni

    1. dipeptidase activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. metallopeptidase activity Source: UniProtKB-KW
    4. omega peptidase activity Source: SGD
    5. peptidase activity Source: SGD

    GO - Biological processi

    1. glutathione catabolic process Source: SGD

    Keywords - Molecular functioni

    Dipeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29201-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable di- and tripeptidase DUG2 (EC:3.4.-.-)
    Alternative name(s):
    Deficient in utilization of glutathione protein 2
    GSH degradosomal complex subunit DUG2
    Gene namesi
    Name:DUG2
    Ordered Locus Names:YBR281C
    ORF Names:YBR2018
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR281c.
    SGDiS000000485. DUG2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 878878Probable di- and tripeptidase DUG2PRO_0000051469Add
    BLAST

    Proteomic databases

    MaxQBiP38149.
    PaxDbiP38149.
    PeptideAtlasiP38149.

    Expressioni

    Gene expression databases

    GenevestigatoriP38149.

    Interactioni

    Subunit structurei

    Component of the GSH degradosomal complex composed of at least DUG1, DUG2 and DUG3.1 Publication

    Protein-protein interaction databases

    BioGridi32975. 19 interactions.
    DIPiDIP-1612N.
    IntActiP38149. 4 interactions.
    MINTiMINT-399156.
    STRINGi4932.YBR281C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38149.
    SMRiP38149. Positions 73-100, 317-401, 423-875.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati18 – 5740WD 1Add
    BLAST
    Repeati68 – 10740WD 2Add
    BLAST
    Repeati235 – 27440WD 3Add
    BLAST
    Repeati282 – 32241WD 4Add
    BLAST
    Repeati362 – 40544WD 5Add
    BLAST
    Repeati608 – 65144WD 6Add
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase M20A family.Curated
    Contains 6 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG0624.
    HOGENOMiHOG000183136.
    KOiK14262.
    OMAiPAAHLPC.
    OrthoDBiEOG7NW6JF.

    Family and domain databases

    Gene3Di2.130.10.10. 2 hits.
    3.30.70.360. 1 hit.
    InterProiIPR020472. G-protein_beta_WD-40_rep.
    IPR017149. GSH_degradosome_DUG2.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    PF00400. WD40. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037237. Peptidase_WD_repeats_DUG2. 1 hit.
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00320. WD40. 4 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 2 hits.
    SSF55031. SSF55031. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38149-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYDSRGVALH SELIHRWNHA FSILSIVAFP KKRLLFAGSQ DSKILVFDLP    50
    TYNLIHTIRL GESQEETHTR SSVLCLTGSE DENFLFSGGA DSLVRIWSIG 100
    EKTIRDDFLP VTEIATVYSV TDIGDIFSLA YLDSLETIVF GCQNASLLYV 150
    ENLIQKIEKK SSDGVENINK LPHRRYDKFF DSLGPTGYSS NSLSQTSLTS 200
    LQENCGAAII EVPSENIIKY AHYGFIYSIN KLCPRFNQLL EKSSRTSGAE 250
    HIISSAGDGI SKLWEFSKDK GQNTVKISLI NDKIDNEDSV ISQTIEFPFL 300
    YCGLTDGIIK IWDLNTQQII STLKTKHESD VISISVYMDH VFAIDESGIT 350
    HFYQNQVNHW NPQQGKILSS EIFSKSNAGS VSLLTGGSDG SLTLWDITSL 400
    LSAVPLSSNS PINASSTLQT TNLWAAYQSA SLNNEEMLNT LRELISFQTV 450
    SQSKDTTNTL SLRRCAIYLQ QLFLKFGATN SQLFPLPDGG NPVVFAYFQG 500
    NGKVSQVKGA KKKRILWYGH YDVISSGNTF NWNTDPFTLT CENGYLKGRG 550
    VSDNKGPLVS AIHSVAYLFQ QGELVNDVVF LVEGSEEIGS ASLKQVCEKY 600
    HDIIGKDIDW ILLSNSTWVD QEHPCLNYGL RGVINAQIKV WSDKPDGHSG 650
    LNGGVYDEPM VNLVKIVSKL QNEQNEIMIP NFYSPLKDLT EEEYQRFQKI 700
    TELANIDENT TVQDLITNWT KPSLSMTTVK FSGPGNITVI PKSVTMGISI 750
    RLVPEQSVEQ VKRDLKAYLE ESFKQLKSQN HLEIKVLNEA EGWLGDPTNH 800
    AYQILKDEIT TAWDVEPLLV REGGSISCLR MLERIFDAPA VQIPCGQSTD 850
    NGHLANENLR IKNWSNLTEI LSKVFNRL 878
    Length:878
    Mass (Da):98,071
    Last modified:October 1, 1994 - v1
    Checksum:iA24C10BB4E94C344
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76053 Genomic DNA. Translation: CAA53644.1.
    Z36150 Genomic DNA. Translation: CAA85245.1.
    BK006936 Genomic DNA. Translation: DAA07396.1.
    PIRiS44543.
    RefSeqiNP_009840.3. NM_001178629.3.

    Genome annotation databases

    EnsemblFungiiYBR281C; YBR281C; YBR281C.
    GeneIDi852584.
    KEGGisce:YBR281C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76053 Genomic DNA. Translation: CAA53644.1 .
    Z36150 Genomic DNA. Translation: CAA85245.1 .
    BK006936 Genomic DNA. Translation: DAA07396.1 .
    PIRi S44543.
    RefSeqi NP_009840.3. NM_001178629.3.

    3D structure databases

    ProteinModelPortali P38149.
    SMRi P38149. Positions 73-100, 317-401, 423-875.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32975. 19 interactions.
    DIPi DIP-1612N.
    IntActi P38149. 4 interactions.
    MINTi MINT-399156.
    STRINGi 4932.YBR281C.

    Proteomic databases

    MaxQBi P38149.
    PaxDbi P38149.
    PeptideAtlasi P38149.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR281C ; YBR281C ; YBR281C .
    GeneIDi 852584.
    KEGGi sce:YBR281C.

    Organism-specific databases

    CYGDi YBR281c.
    SGDi S000000485. DUG2.

    Phylogenomic databases

    eggNOGi COG0624.
    HOGENOMi HOG000183136.
    KOi K14262.
    OMAi PAAHLPC.
    OrthoDBi EOG7NW6JF.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29201-MONOMER.

    Miscellaneous databases

    NextBioi 971733.

    Gene expression databases

    Genevestigatori P38149.

    Family and domain databases

    Gene3Di 2.130.10.10. 2 hits.
    3.30.70.360. 1 hit.
    InterProi IPR020472. G-protein_beta_WD-40_rep.
    IPR017149. GSH_degradosome_DUG2.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    PF00400. WD40. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037237. Peptidase_WD_repeats_DUG2. 1 hit.
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00320. WD40. 4 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 2 hits.
    SSF55031. SSF55031. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 2 hits.
    PS50082. WD_REPEATS_2. 2 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
      Holmstroem K., Brandt T., Kallesoe T.
      Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "The alternative pathway of glutathione degradation is mediated by a novel protein complex involving three new genes in Saccharomyces cerevisiae."
      Ganguli D., Kumar C., Bachhawat A.K.
      Genetics 175:1137-1151(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE GSH DEGRADOSOMAL COMPLEX, SUBCELLULAR LOCATION.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDUG2_YEAST
    AccessioniPrimary (citable) accession number: P38149
    Secondary accession number(s): D6VQS6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1940 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3