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Protein

Dual specificity protein phosphatase PPS1

Gene

PPS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei725 – 7251Phosphocysteine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • inactivation of MAPK activity Source: SGD
  • nuclear DNA replication Source: SGD
  • protein dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle

Enzyme and pathway databases

BioCyciYEAST:G3O-29197-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase PPS1 (EC:3.1.3.16, EC:3.1.3.48)
Gene namesi
Name:PPS1
Ordered Locus Names:YBR276C
ORF Names:YBR2013
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome II

Organism-specific databases

CYGDiYBR276c.
EuPathDBiFungiDB:YBR276C.
SGDiS000000480. PPS1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: GOC
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 807807Dual specificity protein phosphatase PPS1PRO_0000094921Add
BLAST

Proteomic databases

MaxQBiP38148.
PaxDbiP38148.
PeptideAtlasiP38148.

Interactioni

Protein-protein interaction databases

BioGridi32971. 22 interactions.
DIPiDIP-6546N.
IntActiP38148. 2 interactions.
MINTiMINT-702219.
STRINGi4932.YBR276C.

Structurei

3D structure databases

ProteinModelPortaliP38148.
SMRiP38148. Positions 646-777.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni593 – 807215CatalyticAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2453.
HOGENOMiHOG000115673.
InParanoidiP38148.
KOiK04459.
OrthoDBiEOG7R5725.

Family and domain databases

Gene3Di3.90.190.10. 3 hits.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 2 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38148-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLEVPSITP GELHDLMRLH QDAEWPECKK MFPWAHDISF GQPPDFPHSL
60 70 80 90 100
AIVKSQSDAN NSALLRNSLE VNDIFQSWKV RTSFHREGDT CETGNDSNGF
110 120 130 140 150
QYPNNTKELL NLLKFQIRQL ELQVDDVALE NAATYCHNHS ILPFLKVDPR
160 170 180 190 200
GLSLELKRYS RNKVGSNTTL KRSGQDVWGR RGLFRRFDLQ CAKMIEMVDN
210 220 230 240 250
IVIYCSRTGG STDMQTESAP ACSHEGNCPN CTTLALLLQI CLMFVQKGYV
260 270 280 290 300
GSGGSLYKTN LFICTYQNFN TDIPQTLIGT PLLDNEFFKN NTPLNLCSSP
310 320 330 340 350
SEIVCFNNVD KNMVLCEKLE LNKLTSATRL EETGLICGNT TDWHNYQIIK
360 370 380 390 400
KNNISLTHRF EENTSIVNLK SLNYDTDNPT TSISQLYNIP NTKEVWKLII
410 420 430 440 450
KCTSNSQMPS LTKIRTYLDL LLDDDASKSQ EHLHLTFPAS GSIGLGNLNI
460 470 480 490 500
QSVEILLNVC YLIFQVSQVQ ELLTFMYCED GYTETSLLLT AYIIFHFNIP
510 520 530 540 550
LQDALLRIHP RPFFLFPSDL QILGHLQPVL REFSPQNGSN LKLYANALKF
560 570 580 590 600
RDKSFQLHIS SELFSSIFFM KIPLESNFVN LKGPLPSRIL RHLYLGSLDH
610 620 630 640 650
AQNPALLKSL GITHIVSVGE VVSWTLNKDK IAHPVRPHRA ITMTNTNEVA
660 670 680 690 700
GNTTCNKSRN RADTVVSDKQ ENGSNVVISE NSGFQICQIE NLDDNGKDPL
710 720 730 740 750
FHQIDKVLDF ISNSEATGGK VLVHCMVGVS RSATVCIAEC MRYLQCDLAS
760 770 780 790 800
AYLFVRVRRL NVIIQPNLFF VYELFKWWKK HYNREKDKTM DWHIICRGIA

EVNMKYT
Length:807
Mass (Da):91,685
Last modified:October 1, 1994 - v1
Checksum:i17840CF7256EF785
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76053 Genomic DNA. Translation: CAA53639.1.
Z36145 Genomic DNA. Translation: CAA85239.1.
BK006936 Genomic DNA. Translation: DAA07392.1.
PIRiS44538.
RefSeqiNP_009835.3. NM_001178624.3.

Genome annotation databases

EnsemblFungiiYBR276C; YBR276C; YBR276C.
GeneIDi852579.
KEGGisce:YBR276C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76053 Genomic DNA. Translation: CAA53639.1.
Z36145 Genomic DNA. Translation: CAA85239.1.
BK006936 Genomic DNA. Translation: DAA07392.1.
PIRiS44538.
RefSeqiNP_009835.3. NM_001178624.3.

3D structure databases

ProteinModelPortaliP38148.
SMRiP38148. Positions 646-777.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32971. 22 interactions.
DIPiDIP-6546N.
IntActiP38148. 2 interactions.
MINTiMINT-702219.
STRINGi4932.YBR276C.

Proteomic databases

MaxQBiP38148.
PaxDbiP38148.
PeptideAtlasiP38148.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR276C; YBR276C; YBR276C.
GeneIDi852579.
KEGGisce:YBR276C.

Organism-specific databases

CYGDiYBR276c.
EuPathDBiFungiDB:YBR276C.
SGDiS000000480. PPS1.

Phylogenomic databases

eggNOGiCOG2453.
HOGENOMiHOG000115673.
InParanoidiP38148.
KOiK04459.
OrthoDBiEOG7R5725.

Enzyme and pathway databases

BioCyciYEAST:G3O-29197-MONOMER.

Miscellaneous databases

NextBioi971721.
PROiP38148.

Family and domain databases

Gene3Di3.90.190.10. 3 hits.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 2 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
    Holmstroem K., Brandt T., Kallesoe T.
    Yeast 10:S47-S62(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The PPS1 gene of Saccharomyces cerevisiae codes for a dual specificity protein phosphatase with a role in the DNA synthesis phase of the cell cycle."
    Ernsting B.R., Dixon J.E.
    J. Biol. Chem. 272:9332-9343(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPPS1_YEAST
AccessioniPrimary (citable) accession number: P38148
Secondary accession number(s): D6VQS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 24, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.