Reviewed, UniProtKB/Swiss-Prot P38148 (PPS1_YEAST)

Names and origin

Protein names

Recommended name:
    Dual specificity protein phosphatase PPS1
    EC=3.1.3.48
    EC=3.1.3.16

Gene names

Name:	PPS1
Ordered Locus Names:	YBR276C
ORF Names:	YBR2013

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Protein phosphatase with specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle. Ref.3
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Ontologies

Keywords
Biological process	Cell cycle
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid dephosphorylation Ref.3 Inferred from direct assay. Source: SGD regulation of S phase of mitotic cell cycle Ref.3 Inferred from genetic interaction. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro protein tyrosine/threonine phosphatase activity Ref.3 Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ADE13	Q05911	1	EBI-21161,EBI-14263

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 807	807	Dual specificity protein phosphatase PPS1		PRO_0000094921
Regions
	Region	593 – 807	215	Catalytic
Sites
	Active site	725	1	Phosphocysteine intermediate By similarity
Amino acid modifications
	Modified residue	664	1	Phosphothreonine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P38148-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 17840CF7256EF785	FASTA	807	91,685
	10 20 30 40 50 60 MVLEVPSITP GELHDLMRLH QDAEWPECKK MFPWAHDISF GQPPDFPHSL AIVKSQSDAN 70 80 90 100 110 120 NSALLRNSLE VNDIFQSWKV RTSFHREGDT CETGNDSNGF QYPNNTKELL NLLKFQIRQL 130 140 150 160 170 180 ELQVDDVALE NAATYCHNHS ILPFLKVDPR GLSLELKRYS RNKVGSNTTL KRSGQDVWGR 190 200 210 220 230 240 RGLFRRFDLQ CAKMIEMVDN IVIYCSRTGG STDMQTESAP ACSHEGNCPN CTTLALLLQI 250 260 270 280 290 300 CLMFVQKGYV GSGGSLYKTN LFICTYQNFN TDIPQTLIGT PLLDNEFFKN NTPLNLCSSP 310 320 330 340 350 360 SEIVCFNNVD KNMVLCEKLE LNKLTSATRL EETGLICGNT TDWHNYQIIK KNNISLTHRF 370 380 390 400 410 420 EENTSIVNLK SLNYDTDNPT TSISQLYNIP NTKEVWKLII KCTSNSQMPS LTKIRTYLDL 430 440 450 460 470 480 LLDDDASKSQ EHLHLTFPAS GSIGLGNLNI QSVEILLNVC YLIFQVSQVQ ELLTFMYCED 490 500 510 520 530 540 GYTETSLLLT AYIIFHFNIP LQDALLRIHP RPFFLFPSDL QILGHLQPVL REFSPQNGSN 550 560 570 580 590 600 LKLYANALKF RDKSFQLHIS SELFSSIFFM KIPLESNFVN LKGPLPSRIL RHLYLGSLDH 610 620 630 640 650 660 AQNPALLKSL GITHIVSVGE VVSWTLNKDK IAHPVRPHRA ITMTNTNEVA GNTTCNKSRN 670 680 690 700 710 720 RADTVVSDKQ ENGSNVVISE NSGFQICQIE NLDDNGKDPL FHQIDKVLDF ISNSEATGGK 730 740 750 760 770 780 VLVHCMVGVS RSATVCIAEC MRYLQCDLAS AYLFVRVRRL NVIIQPNLFF VYELFKWWKK 790 800 HYNREKDKTM DWHIICRGIA EVNMKYT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 32,420 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae." Holmstroem K., Brandt T., Kallesoe T. Yeast 10:S47-S62(1994) [PubMed: 8091861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The PPS1 gene of Saccharomyces cerevisiae codes for a dual specificity protein phosphatase with a role in the DNA synthesis phase of the cell cycle." Ernsting B.R., Dixon J.E. J. Biol. Chem. 272:9332-9343(1997) [PubMed: 9083070] [Abstract] Cited for: FUNCTION.
[4]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-664, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X76053 Genomic DNA. Translation: CAA53639.1. Z36145 Genomic DNA. Translation: CAA85239.1.
PIR	S44538.
RefSeq	NP_009835.1.
3D structure databases
HSSP	HSSP built from PDB template 1M3G based on UniProtKB Q05923.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6546N.
IntAct	P38148. 2 interactions.
Proteomic databases
PeptideAtlas	P38148.
PRIDE	P38148.
Genome annotation databases
Ensembl	YBR276C. Saccharomyces cerevisiae. [Contig view]
GeneID	852579.
GenomeReviews	Gene locus YBR276C in contig Y13134_GR.
KEGG	sce:YBR276C.
NMPDR	fig|4932.3.peg.551.
Organism-specific databases
CYGD	YBR276c.
SGD	S000000480. PPS1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P38148.
Enzyme and pathway databases
BRENDA	3.1.3.16. 250. 3.1.3.48. 250.
Gene expression databases
ArrayExpress	P38148.
GermOnline	YBR276C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000387. Tyr_Pase. IPR016130. Tyr_Pase_AS. IPR000340. Tyr_Pase_dual_specific. [Graphical view]
Pfam	PF00782. DSPc. 2 hits. [Graphical view]
SMART	SM00195. DSPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	971721.

Entry information

Entry name

PPS1_YEAST

Accession

Primary (citable) accession number: P38148

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)