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Protein

Serine/threonine-protein kinase CHK1

Gene

CHK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit.

Miscellaneous

Present with 2530 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45ATPPROSITE-ProRule annotation1
Active sitei142Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi21 – 29ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • DNA damage checkpoint Source: SGD
  • mitotic G2 DNA damage checkpoint Source: GO_Central
  • peptidyl-serine phosphorylation Source: GO_Central
  • peptidyl-threonine phosphorylation Source: GO_Central
  • protein phosphorylation Source: SGD
  • replication fork protection Source: SGD
  • signal transduction involved in mitotic DNA damage checkpoint Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, DNA damage
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29195-MONOMER
BRENDAi2.7.11.1 984
ReactomeiR-SCE-176187 Activation of ATR in response to replication stress
R-SCE-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase CHK1 (EC:2.7.11.1)
Alternative name(s):
Checkpoint kinase 1
Gene namesi
Name:CHK1
Ordered Locus Names:YBR274W
ORF Names:YBR1742
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR274W
SGDiS000000478 CHK1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858571 – 527Serine/threonine-protein kinase CHK1Add BLAST527

Proteomic databases

MaxQBiP38147
PaxDbiP38147
PRIDEiP38147

PTM databases

iPTMnetiP38147

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q006842EBI-4593,EBI-4192

Protein-protein interaction databases

BioGridi32969, 420 interactors
DIPiDIP-1253N
IntActiP38147, 37 interactors
MINTiP38147
STRINGi4932.YBR274W

Structurei

3D structure databases

ProteinModelPortaliP38147
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 281Protein kinasePROSITE-ProRule annotationAdd BLAST267

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00680000099954
HOGENOMiHOG000216658
InParanoidiP38147
KOiK02216
OMAiFAVKFIH
OrthoDBiEOG092C35VZ

Family and domain databases

CDDicd14069 STKc_Chk1, 1 hit
InterProiView protein in InterPro
IPR034670 Chk1_catalytic_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P38147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSQVSPLP HIKDVVLGDT VGQGAFACVK NAHLQMDPSI ILAVKFIHVP
60 70 80 90 100
TCKKMGLSDK DITKEVVLQS KCSKHPNVLR LIDCNVSKEY MWIILEMADG
110 120 130 140 150
GDLFDKIEPD VGVDSDVAQF YFQQLVSAIN YLHVECGVAH RDIKPENILL
160 170 180 190 200
DKNGNLKLAD FGLASQFRRK DGTLRVSMDQ RGSPPYMAPE VLYSEEGYYA
210 220 230 240 250
DRTDIWSIGI LLFVLLTGQT PWELPSLENE DFVFFIENDG NLNWGPWSKI
260 270 280 290 300
EFTHLNLLRK ILQPDPNKRV TLKALKLHPW VLRRASFSGD DGLCNDPELL
310 320 330 340 350
AKKLFSHLKV SLSNENYLKF TQDTNSNNRY ISTQPIGNEL AELEHDSMHF
360 370 380 390 400
QTVSNTQRAF TSYDSNTNYN SGTGMTQEAK WTQFISYDIA ALQFHSDEND
410 420 430 440 450
CNELVKRHLQ FNPNKLTKFY TLQPMDVLLP ILEKALNLSQ IRVKPDLFAN
460 470 480 490 500
FERLCELLGY DNVFPLIINI KTKSNGGYQL CGSISIIKIE EELKSVGFER
510 520
KTGDPLEWRR LFKKISTICR DIILIPN
Length:527
Mass (Da):60,017
Last modified:October 1, 1994 - v1
Checksum:i0BD66B74FE079DCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117345 Genomic DNA Translation: AAD17231.1
Z36143 Genomic DNA Translation: CAA85237.1
BK006936 Genomic DNA Translation: DAA07390.1
PIRiS46155
RefSeqiNP_009833.1, NM_001178622.1

Genome annotation databases

EnsemblFungiiYBR274W; YBR274W; YBR274W
GeneIDi852577
KEGGisce:YBR274W

Similar proteinsi

Entry informationi

Entry nameiCHK1_YEAST
AccessioniPrimary (citable) accession number: P38147
Secondary accession number(s): D6VQS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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