Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase CHK1

Gene

CHK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451ATPPROSITE-ProRule annotation
Active sitei142 – 1421Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi21 – 299ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • DNA damage checkpoint Source: SGD
  • protein phosphorylation Source: SGD
  • replication fork protection Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, DNA damage

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29195-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-176187. Activation of ATR in response to replication stress.
R-SCE-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase CHK1 (EC:2.7.11.1)
Alternative name(s):
Checkpoint kinase 1
Gene namesi
Name:CHK1
Ordered Locus Names:YBR274W
ORF Names:YBR1742
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR274W.
SGDiS000000478. CHK1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Serine/threonine-protein kinase CHK1PRO_0000085857Add
BLAST

Proteomic databases

MaxQBiP38147.
PeptideAtlasiP38147.

PTM databases

iPTMnetiP38147.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC14Q006842EBI-4593,EBI-4192

Protein-protein interaction databases

BioGridi32969. 167 interactions.
DIPiDIP-1253N.
IntActiP38147. 27 interactions.
MINTiMINT-387530.

Structurei

3D structure databases

ProteinModelPortaliP38147.
SMRiP38147. Positions 16-378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 281267Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00680000099954.
HOGENOMiHOG000216658.
InParanoidiP38147.
KOiK02216.
OMAiRGSPPYM.
OrthoDBiEOG7VB2Q7.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSQVSPLP HIKDVVLGDT VGQGAFACVK NAHLQMDPSI ILAVKFIHVP
60 70 80 90 100
TCKKMGLSDK DITKEVVLQS KCSKHPNVLR LIDCNVSKEY MWIILEMADG
110 120 130 140 150
GDLFDKIEPD VGVDSDVAQF YFQQLVSAIN YLHVECGVAH RDIKPENILL
160 170 180 190 200
DKNGNLKLAD FGLASQFRRK DGTLRVSMDQ RGSPPYMAPE VLYSEEGYYA
210 220 230 240 250
DRTDIWSIGI LLFVLLTGQT PWELPSLENE DFVFFIENDG NLNWGPWSKI
260 270 280 290 300
EFTHLNLLRK ILQPDPNKRV TLKALKLHPW VLRRASFSGD DGLCNDPELL
310 320 330 340 350
AKKLFSHLKV SLSNENYLKF TQDTNSNNRY ISTQPIGNEL AELEHDSMHF
360 370 380 390 400
QTVSNTQRAF TSYDSNTNYN SGTGMTQEAK WTQFISYDIA ALQFHSDEND
410 420 430 440 450
CNELVKRHLQ FNPNKLTKFY TLQPMDVLLP ILEKALNLSQ IRVKPDLFAN
460 470 480 490 500
FERLCELLGY DNVFPLIINI KTKSNGGYQL CGSISIIKIE EELKSVGFER
510 520
KTGDPLEWRR LFKKISTICR DIILIPN
Length:527
Mass (Da):60,017
Last modified:October 1, 1994 - v1
Checksum:i0BD66B74FE079DCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117345 Genomic DNA. Translation: AAD17231.1.
Z36143 Genomic DNA. Translation: CAA85237.1.
BK006936 Genomic DNA. Translation: DAA07390.1.
PIRiS46155.
RefSeqiNP_009833.1. NM_001178622.1.

Genome annotation databases

EnsemblFungiiYBR274W; YBR274W; YBR274W.
GeneIDi852577.
KEGGisce:YBR274W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117345 Genomic DNA. Translation: AAD17231.1.
Z36143 Genomic DNA. Translation: CAA85237.1.
BK006936 Genomic DNA. Translation: DAA07390.1.
PIRiS46155.
RefSeqiNP_009833.1. NM_001178622.1.

3D structure databases

ProteinModelPortaliP38147.
SMRiP38147. Positions 16-378.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32969. 167 interactions.
DIPiDIP-1253N.
IntActiP38147. 27 interactions.
MINTiMINT-387530.

PTM databases

iPTMnetiP38147.

Proteomic databases

MaxQBiP38147.
PeptideAtlasiP38147.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR274W; YBR274W; YBR274W.
GeneIDi852577.
KEGGisce:YBR274W.

Organism-specific databases

EuPathDBiFungiDB:YBR274W.
SGDiS000000478. CHK1.

Phylogenomic databases

GeneTreeiENSGT00680000099954.
HOGENOMiHOG000216658.
InParanoidiP38147.
KOiK02216.
OMAiRGSPPYM.
OrthoDBiEOG7VB2Q7.

Enzyme and pathway databases

BioCyciYEAST:G3O-29195-MONOMER.
BRENDAi2.7.11.1. 984.
ReactomeiR-SCE-176187. Activation of ATR in response to replication stress.
R-SCE-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-SCE-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.

Miscellaneous databases

PROiP38147.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Control of the DNA damage checkpoint by chk1 and rad53 protein kinases through distinct mechanisms."
    Sanchez Y., Bachant J., Wang H., Hu F., Liu D., Tetzlaff M., Elledge S.J.
    Science 286:1166-1171(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCHK1_YEAST
AccessioniPrimary (citable) accession number: P38147
Secondary accession number(s): D6VQS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 8, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2530 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.