P38147 (CHK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase CHK1
EC=2.7.11.1

Alternative name(s):
Checkpoint kinase 1

Gene names

Name:	CHK1
Ordered Locus Names:	YBR274W
ORF Names:	YBR1742

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Nucleus By similarity.
Miscellaneous	Present with 2530 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle DNA damage
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA damage checkpoint Inferred from mutant phenotype. Source: SGD cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from physical interaction. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC14	Q00684	2	EBI-4593,EBI-4192

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	Serine/threonine-protein kinase CHK1		PRO_0000085857
Regions
	Domain	15 – 281	267	Protein kinase
	Nucleotide binding	21 – 29	9	ATP By similarity
Sites
	Active site	142	1	Proton acceptor By similarity
	Binding site	45	1	ATP By similarity
Amino acid modifications
	Modified residue	373	1	Phosphothreonine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P38147 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 0BD66B74FE079DCA	FASTA	527	60,017
	10 20 30 40 50 60 MSLSQVSPLP HIKDVVLGDT VGQGAFACVK NAHLQMDPSI ILAVKFIHVP TCKKMGLSDK 70 80 90 100 110 120 DITKEVVLQS KCSKHPNVLR LIDCNVSKEY MWIILEMADG GDLFDKIEPD VGVDSDVAQF 130 140 150 160 170 180 YFQQLVSAIN YLHVECGVAH RDIKPENILL DKNGNLKLAD FGLASQFRRK DGTLRVSMDQ 190 200 210 220 230 240 RGSPPYMAPE VLYSEEGYYA DRTDIWSIGI LLFVLLTGQT PWELPSLENE DFVFFIENDG 250 260 270 280 290 300 NLNWGPWSKI EFTHLNLLRK ILQPDPNKRV TLKALKLHPW VLRRASFSGD DGLCNDPELL 310 320 330 340 350 360 AKKLFSHLKV SLSNENYLKF TQDTNSNNRY ISTQPIGNEL AELEHDSMHF QTVSNTQRAF 370 380 390 400 410 420 TSYDSNTNYN SGTGMTQEAK WTQFISYDIA ALQFHSDEND CNELVKRHLQ FNPNKLTKFY 430 440 450 460 470 480 TLQPMDVLLP ILEKALNLSQ IRVKPDLFAN FERLCELLGY DNVFPLIINI KTKSNGGYQL 490 500 510 520 CGSISIIKIE EELKSVGFER KTGDPLEWRR LFKKISTICR DIILIPN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Control of the DNA damage checkpoint by chk1 and rad53 protein kinases through distinct mechanisms." Sanchez Y., Bachant J., Wang H., Hu F., Liu D., Tetzlaff M., Elledge S.J. Science 286:1166-1171(1999) [PubMed: 10550056] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-373, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF117345 Genomic DNA. Translation: AAD17231.1. Z36143 Genomic DNA. Translation: CAA85237.1. BK006936 Genomic DNA. Translation: DAA07390.1.
PIR	S46155.
RefSeq	NP_009833.1. NM_001178622.1.
3D structure databases
ProteinModelPortal	P38147.
SMR	P38147. Positions 8-284.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1253N.
IntAct	P38147. 29 interactions.
MINT	MINT-387530.
STRING	P38147.
Proteomic databases
PeptideAtlas	P38147.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR274W; YBR274W; YBR274W.
GeneID	852577.
KEGG	sce:YBR274W.
NMPDR	fig|4932.3.peg.549.
Organism-specific databases
CYGD	YBR274w.
SGD	S000000478. CHK1.
Phylogenomic databases
eggNOG	fuNOG04644.
GeneTree	EFGT00070000008711.
HOGENOM	HBG333050.
OMA	PLEWRRF.
OrthoDB	EOG4VX5DG.
Enzyme and pathway databases
BRENDA	2.7.11.1. 984.
Gene expression databases
ArrayExpress	P38147.
Genevestigator	P38147.
GermOnline	YBR274W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K02216.
PANTHER	PTHR24347. PTHR24347. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971715.

Entry information

Entry name

CHK1_YEAST

Accession

Primary (citable) accession number: P38147
Secondary accession number(s): D6VQS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program