RIB5

Functionⁱ

Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.1 Publication

Miscellaneous

Present with 7300 molecules/cell in log phase SD medium.1 Publication

Catalytic activityⁱ

2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.

Pathwayⁱ: riboflavin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

6,7-dimethyl-8-ribityllumazine synthase (RIB4)
Riboflavin synthase (RIB5)

This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	143	Lumazine 2By similarity		1

GO - Molecular functionⁱ

riboflavin synthase activity
Source: SGD
Inferred from direct assayⁱ
- 7814407

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

riboflavin biosynthetic process
Source: SGD
Inferred from mutant phenotypeⁱ
- 7814407

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Riboflavin biosynthesis

Enzyme and pathway databases

BioCycⁱ	MetaCyc:MONOMER3O-75 YEAST:MONOMER3O-75
UniPathwayⁱ	UPA00275; UER00405

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Riboflavin synthase (EC:2.5.1.9) Short name: RS
Gene namesⁱ	Name:RIB5 Ordered Locus Names:YBR256C ORF Names:YBR1724
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome II

Organism-specific databases

EuPathDBⁱ	FungiDB:YBR256C
Saccharomyces Genome Database More...SGDⁱ	S000000460 RIB5

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000068174	1 – 238	Riboflavin synthaseAdd BLAST		238

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	95	PhosphoserineCombined sources		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P38145
PaxDbⁱ	P38145
PRIDEⁱ	P38145

PTM databases

iPTMnetⁱ	P38145

iPTMnetⁱ

P38145

Interactionⁱ

Subunit structureⁱ

Homotrimer.By similarity

Protein-protein interaction databases

BioGridⁱ	32952, 139 interactors
Molecular INTeraction database More...MINTⁱ	P38145
STRINGⁱ	4932.YBR256C

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P38145
SMRⁱ	P38145
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Repeatⁱ	1 – 103	Lumazine-binding 1Add BLAST		103
Repeatⁱ	104 – 205	Lumazine-binding 2Add BLAST		102

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	4 – 6	Lumazine 1 bindingBy similarity	3
Regionⁱ	54 – 56	Lumazine 2 binding; shared with one trimeric partnerBy similarity	3
Regionⁱ	68 – 73	Lumazine 2 binding; shared with one trimeric partnerBy similarity	6
Regionⁱ	107 – 109	Lumazine 2 binding; shared with one trimeric partnerBy similarity	3
Regionⁱ	152 – 154	Lumazine 1 bindingBy similarity	3
Regionⁱ	170 – 175	Lumazine 1 bindingBy similarity	6

Keywords - Domainⁱ

Repeat

Phylogenomic databases

HOGENOMⁱ	HOG000151757
InParanoidⁱ	P38145
KEGG Orthology (KO) More...KOⁱ	K00793
OMAⁱ	HFVTGHV
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C3Z05

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00402 Riboflavin_synthase_like, 1 hit
Gene3Dⁱ	2.40.30.20, 2 hits
InterProⁱ	View protein in InterPro IPR023366 ATP_synth_asu-like_sf IPR001783 Lumazine-bd IPR026017 Lumazine-bd_dom IPR017938 Riboflavin_synthase-like_b-brl
PANTHERⁱ	PTHR21098 PTHR21098, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00677 Lum_binding, 2 hits
PIRSFⁱ	PIRSF000498 Riboflavin_syn_A, 1 hit
SUPFAMⁱ	SSF63380 SSF63380, 2 hits
TIGRFAMsⁱ	TIGR00187 ribE, 1 hit
PROSITEⁱ	View protein in PROSITE PS51177 LUMAZINE_BIND, 2 hits

Sequenceⁱ

Sequence statusⁱ: Complete.

P38145-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MFTGIVECMG TVLENNPYDD SESGGQGVSI TIGNAGSILT DCHVGDSIAV 
        60         70         80         90        100
NGVCLTVTEF NNDSFKVGIS PETIKRSNVA SWIQGTQVNL ERAVSQDVRF 
       110        120        130        140        150
GGHYVQGHVD TVANIVSRRP EGNSIIFGFQ LRDQEYFKYI VEKGFICIDG 
       160        170        180        190        200
TSLTIIKVDP LSQGGAFYIS MIKHTQDNVI MPLKKIGDEV NIEVDLTGKI 
       210        220        230 
IEKQILLTLE NQISKKDSTL NTMISNIIEE KVRNYLNK

Length:238

Mass (Da):26,196

Last modified:October 1, 1994 - v1

Checksum:ⁱEA57EAD542D46897

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z21621 Genomic DNA Translation: CAA79745.1 X70529 Genomic DNA Translation: CAA49920.1 Z36125 Genomic DNA Translation: CAA85219.1 BK006936 Genomic DNA Translation: DAA07373.1
PIRⁱ	S34072
NCBI Reference Sequences More...RefSeqⁱ	NP_009815.1, NM_001178604.1

Genome annotation databases

EnsemblFungiⁱ	YBR256C; YBR256C; YBR256C
GeneIDⁱ	852559
KEGGⁱ	sce:YBR256C

Protein	Similar proteins		Species	Score	Length
P38145	Riboflavin synthase alpha chain		YEAS1		238
Rib5p		YEASA		238
Rib5p		YEAS8		238
RIB5p Riboflavin synthase		Saccharomyces sp. 'boulardii'		238
Rib5p		YEASC		238

Protein	Similar proteins		Species	Score	Length
P38145	Riboflavin synthase alpha chain		YEAS1		238
Rib5p		YEASA		238
Rib5p		YEAS8		238
RIB5p Riboflavin synthase		Saccharomyces sp. 'boulardii'		238
Rib5p		YEASC		238
+10

Protein	Similar proteins		Species	Score	Length
P38145	Riboflavin synthase alpha chain		YEAS1		238
Rib5p		YEASA		238
Rib5p		YEAS8		238
RIB5p Riboflavin synthase		Saccharomyces sp. 'boulardii'		238
Rib5p		YEASC		238
+51

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z21621 Genomic DNA Translation: CAA79745.1 X70529 Genomic DNA Translation: CAA49920.1 Z36125 Genomic DNA Translation: CAA85219.1 BK006936 Genomic DNA Translation: DAA07373.1
PIRⁱ	S34072
NCBI Reference Sequences More...RefSeqⁱ	NP_009815.1, NM_001178604.1

3D structure databases

ProteinModelPortalⁱ	P38145
SMRⁱ	P38145
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	32952, 139 interactors
Molecular INTeraction database More...MINTⁱ	P38145
STRINGⁱ	4932.YBR256C

PTM databases

iPTMnetⁱ	P38145

iPTMnetⁱ

P38145

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P38145
PaxDbⁱ	P38145
PRIDEⁱ	P38145

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YBR256C; YBR256C; YBR256C
GeneIDⁱ	852559
KEGGⁱ	sce:YBR256C

Organism-specific databases

EuPathDBⁱ	FungiDB:YBR256C
Saccharomyces Genome Database More...SGDⁱ	S000000460 RIB5

Phylogenomic databases

HOGENOMⁱ	HOG000151757
InParanoidⁱ	P38145
KEGG Orthology (KO) More...KOⁱ	K00793
OMAⁱ	HFVTGHV
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C3Z05

Enzyme and pathway databases

UniPathwayⁱ	UPA00275; UER00405
BioCycⁱ	MetaCyc:MONOMER3O-75 YEAST:MONOMER3O-75

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P38145

PROⁱ

PR:P38145

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00402 Riboflavin_synthase_like, 1 hit
Gene3Dⁱ	2.40.30.20, 2 hits
InterProⁱ	View protein in InterPro IPR023366 ATP_synth_asu-like_sf IPR001783 Lumazine-bd IPR026017 Lumazine-bd_dom IPR017938 Riboflavin_synthase-like_b-brl
PANTHERⁱ	PTHR21098 PTHR21098, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00677 Lum_binding, 2 hits
PIRSFⁱ	PIRSF000498 Riboflavin_syn_A, 1 hit
SUPFAMⁱ	SSF63380 SSF63380, 2 hits
TIGRFAMsⁱ	TIGR00187 ribE, 1 hit
PROSITEⁱ	View protein in PROSITE PS51177 LUMAZINE_BIND, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RISA_YEAST
Accessionⁱ	P38145Primary (citable) accession number: P38145 Secondary accession number(s): D6VQQ3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
	Last sequence update:	October 1, 1994
	Last modified:	May 23, 2018
	This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase
Protein nomenclature information
List of publications dealing with protein nomenclature and links to specialized websites

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P38145 (RISA_YEAST)

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Riboflavin synthase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: riboflavin biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Pathwayⁱ: riboflavin biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ