RIB5

Functionⁱ

Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.1 Publication

Miscellaneous

Present with 7300 molecules/cell in log phase SD medium.1 Publication

Catalytic activityⁱ

2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.

Pathwayⁱ: riboflavin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:

6,7-dimethyl-8-ribityllumazine synthase (RIB4)
Riboflavin synthase (RIB5)

This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	143	Lumazine 2By similarity		1

GO - Molecular functionⁱ

riboflavin synthase activity
Source: SGD
Inferred from direct assayⁱ
- 7814407

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

riboflavin biosynthetic process
Source: SGD
Inferred from mutant phenotypeⁱ
- 7814407

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Riboflavin biosynthesis

Enzyme and pathway databases

BioCycⁱ	MetaCyc:MONOMER3O-75. YEAST:MONOMER3O-75.
UniPathwayⁱ	UPA00275; UER00405.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Riboflavin synthase (EC:2.5.1.9) Short name: RS
Gene namesⁱ	Name:RIB5 Ordered Locus Names:YBR256C ORF Names:YBR1724
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome II

Organism-specific databases

EuPathDBⁱ	FungiDB:YBR256C.
Saccharomyces Genome Database More...SGDⁱ	S000000460. RIB5.

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000068174	1 – 238	Riboflavin synthaseAdd BLAST		238

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	95	PhosphoserineCombined sources		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P38145.
PaxDbⁱ	P38145.
PRIDEⁱ	P38145.

PTM databases

iPTMnetⁱ	P38145.

iPTMnetⁱ

P38145.

Interactionⁱ

Subunit structureⁱ

Homotrimer.By similarity

Protein-protein interaction databases

BioGridⁱ	32952. 139 interactors.
IntActⁱ	P38145. 1 interactor.
Molecular INTeraction database More...MINTⁱ	P38145.
STRINGⁱ	4932.YBR256C.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P38145.
SMRⁱ	P38145.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Repeatⁱ	1 – 103	Lumazine-binding 1Add BLAST		103
Repeatⁱ	104 – 205	Lumazine-binding 2Add BLAST		102

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	4 – 6	Lumazine 1 bindingBy similarity	3
Regionⁱ	54 – 56	Lumazine 2 binding; shared with one trimeric partnerBy similarity	3
Regionⁱ	68 – 73	Lumazine 2 binding; shared with one trimeric partnerBy similarity	6
Regionⁱ	107 – 109	Lumazine 2 binding; shared with one trimeric partnerBy similarity	3
Regionⁱ	152 – 154	Lumazine 1 bindingBy similarity	3
Regionⁱ	170 – 175	Lumazine 1 bindingBy similarity	6

Keywords - Domainⁱ

Repeat

Phylogenomic databases

HOGENOMⁱ	HOG000151757.
InParanoidⁱ	P38145.
KEGG Orthology (KO) More...KOⁱ	K00793.
OMAⁱ	HFVTGHV.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C3Z05.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00402. Riboflavin_synthase_like. 1 hit.
Gene3Dⁱ	2.40.30.20. 2 hits.
InterProⁱ	View protein in InterPro IPR023366. ATP_synth_asu-like_sf. IPR001783. Lumazine-bd. IPR026017. Lumazine-bd_dom. IPR017938. Riboflavin_synthase-like_b-brl.
PANTHERⁱ	PTHR21098. PTHR21098. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00677. Lum_binding. 2 hits.
PIRSFⁱ	PIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAMⁱ	SSF63380. SSF63380. 2 hits.
TIGRFAMsⁱ	TIGR00187. ribE. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51177. LUMAZINE_BIND. 2 hits.

Sequenceⁱ

Sequence statusⁱ: Complete.

P38145-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MFTGIVECMG TVLENNPYDD SESGGQGVSI TIGNAGSILT DCHVGDSIAV 
        60         70         80         90        100
NGVCLTVTEF NNDSFKVGIS PETIKRSNVA SWIQGTQVNL ERAVSQDVRF 
       110        120        130        140        150
GGHYVQGHVD TVANIVSRRP EGNSIIFGFQ LRDQEYFKYI VEKGFICIDG 
       160        170        180        190        200
TSLTIIKVDP LSQGGAFYIS MIKHTQDNVI MPLKKIGDEV NIEVDLTGKI 
       210        220        230 
IEKQILLTLE NQISKKDSTL NTMISNIIEE KVRNYLNK

Length:238

Mass (Da):26,196

Last modified:October 1, 1994 - v1

Checksum:ⁱEA57EAD542D46897

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z21621 Genomic DNA. Translation: CAA79745.1. X70529 Genomic DNA. Translation: CAA49920.1. Z36125 Genomic DNA. Translation: CAA85219.1. BK006936 Genomic DNA. Translation: DAA07373.1.
PIRⁱ	S34072.
NCBI Reference Sequences More...RefSeqⁱ	NP_009815.1. NM_001178604.1.

Genome annotation databases

EnsemblFungiⁱ	YBR256C; YBR256C; YBR256C.
GeneIDⁱ	852559.
KEGGⁱ	sce:YBR256C.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P38145	E7K9K8 D3UF04 A0A0L8VUW0 N1P9M6 B3LMQ5	Saccharomyces cerevisiae (strain AWRI796) (Baker's yeast) Saccharomyces cerevisiae (strain Lalvin EC1118 / Prise de mousse) (Baker's yeast) Saccharomyces sp. 'boulardii' Saccharomyces cerevisiae (strain CEN.PK113-7D) (Baker's yeast) Saccharomyces cerevisiae (strain RM11-1a) (Baker's yeast)	238	UniRef100_P38145	Cluster: Riboflavin synthase	6

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P38145	E7K9K8 D3UF04 A0A0L8VUW0 N1P9M6 B3LMQ5 A0A250WGE4 C7GMX4 G2W9R1 E7NF55 A6ZLJ6 +6	Saccharomyces cerevisiae (strain AWRI796) (Baker's yeast) Saccharomyces cerevisiae (strain Lalvin EC1118 / Prise de mousse) (Baker's yeast) Saccharomyces sp. 'boulardii' Saccharomyces cerevisiae (strain CEN.PK113-7D) (Baker's yeast) Saccharomyces cerevisiae (strain RM11-1a) (Baker's yeast) Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain JAY291) (Baker's yeast) Saccharomyces cerevisiae (strain Kyokai no. 7 / NBRC 101557) (Baker's yeast) Saccharomyces cerevisiae (strain FostersO) (Baker's yeast) Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) And more	238	UniRef90_P38145	Cluster: Riboflavin synthase	17

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P38145	E7K9K8 D3UF04 A0A0L8VUW0 N1P9M6 B3LMQ5 A6ZLJ6 E7Q155 B5VEK5 E7QBR8 A0A250WGE4 +49	Saccharomyces cerevisiae (strain AWRI796) (Baker's yeast) Saccharomyces cerevisiae (strain Lalvin EC1118 / Prise de mousse) (Baker's yeast) Saccharomyces sp. 'boulardii' Saccharomyces cerevisiae (strain CEN.PK113-7D) (Baker's yeast) Saccharomyces cerevisiae (strain RM11-1a) (Baker's yeast) Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) Saccharomyces cerevisiae (strain FostersB) (Baker's yeast) Saccharomyces cerevisiae (strain AWRI1631) (Baker's yeast) Saccharomyces cerevisiae (strain Zymaflore VL3) (Baker's yeast) Saccharomyces cerevisiae (Baker's yeast) And more	238	UniRef50_P38145	Cluster: Riboflavin synthase	60

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	Z21621 Genomic DNA. Translation: CAA79745.1. X70529 Genomic DNA. Translation: CAA49920.1. Z36125 Genomic DNA. Translation: CAA85219.1. BK006936 Genomic DNA. Translation: DAA07373.1.
PIRⁱ	S34072.
NCBI Reference Sequences More...RefSeqⁱ	NP_009815.1. NM_001178604.1.

3D structure databases

ProteinModelPortalⁱ	P38145.
SMRⁱ	P38145.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	32952. 139 interactors.
IntActⁱ	P38145. 1 interactor.
Molecular INTeraction database More...MINTⁱ	P38145.
STRINGⁱ	4932.YBR256C.

PTM databases

iPTMnetⁱ	P38145.

iPTMnetⁱ

P38145.

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P38145.
PaxDbⁱ	P38145.
PRIDEⁱ	P38145.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YBR256C; YBR256C; YBR256C.
GeneIDⁱ	852559.
KEGGⁱ	sce:YBR256C.

Organism-specific databases

EuPathDBⁱ	FungiDB:YBR256C.
Saccharomyces Genome Database More...SGDⁱ	S000000460. RIB5.

Phylogenomic databases

HOGENOMⁱ	HOG000151757.
InParanoidⁱ	P38145.
KEGG Orthology (KO) More...KOⁱ	K00793.
OMAⁱ	HFVTGHV.
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C3Z05.

Enzyme and pathway databases

UniPathwayⁱ	UPA00275; UER00405.
BioCycⁱ	MetaCyc:MONOMER3O-75. YEAST:MONOMER3O-75.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P38145.

PROⁱ

PR:P38145.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00402. Riboflavin_synthase_like. 1 hit.
Gene3Dⁱ	2.40.30.20. 2 hits.
InterProⁱ	View protein in InterPro IPR023366. ATP_synth_asu-like_sf. IPR001783. Lumazine-bd. IPR026017. Lumazine-bd_dom. IPR017938. Riboflavin_synthase-like_b-brl.
PANTHERⁱ	PTHR21098. PTHR21098. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00677. Lum_binding. 2 hits.
PIRSFⁱ	PIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAMⁱ	SSF63380. SSF63380. 2 hits.
TIGRFAMsⁱ	TIGR00187. ribE. 1 hit.
PROSITEⁱ	View protein in PROSITE PS51177. LUMAZINE_BIND. 2 hits.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RISA_YEAST
Accessionⁱ	P38145Primary (citable) accession number: P38145 Secondary accession number(s): D6VQQ3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
	Last sequence update:	October 1, 1994
	Last modified:	March 28, 2018
	This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P38145 (RISA_YEAST)

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Riboflavin synthase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: riboflavin biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: riboflavin biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ