P38145 (RISA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin synthase
Short name=RS
EC=2.5.1.9

Gene names

Name:	RIB5
Ordered Locus Names:	YBR256C
ORF Names:	YBR1724

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil. Ref.1
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.
Subunit structure	Homotrimer By similarity.
Miscellaneous	Present with 7300 molecules/cell in log phase SD medium.
Sequence similarities	Contains 2 lumazine-binding repeats.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	riboflavin biosynthetic process Inferred from mutant phenotype Ref.1. Source: SGD
Molecular_function	oxidoreductase activity Inferred from electronic annotation. Source: InterPro riboflavin synthase activity Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-2083267,EBI-2083267
ARO8	P53090	1	EBI-2083267,EBI-2042933
IPP1	P00817	1	EBI-2083267,EBI-9338
RNR1	P21524	1	EBI-2083267,EBI-15234
VAS1	P07806-1	1	EBI-2083267,EBI-1009942	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	Riboflavin synthase		PRO_0000068174
Regions
	Repeat	1 – 103	103	Lumazine-binding 1
	Repeat	104 – 205	102	Lumazine-binding 2
	Region	87 – 91	5	Lumazine binding Probable
	Region	189 – 193	5	Lumazine binding Probable
Amino acid modifications
	Modified residue	95	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P38145 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: EA57EAD542D46897	FASTA	238	26,196
	10 20 30 40 50 60 MFTGIVECMG TVLENNPYDD SESGGQGVSI TIGNAGSILT DCHVGDSIAV NGVCLTVTEF 70 80 90 100 110 120 NNDSFKVGIS PETIKRSNVA SWIQGTQVNL ERAVSQDVRF GGHYVQGHVD TVANIVSRRP 130 140 150 160 170 180 EGNSIIFGFQ LRDQEYFKYI VEKGFICIDG TSLTIIKVDP LSQGGAFYIS MIKHTQDNVI 190 200 210 220 230 MPLKKIGDEV NIEVDLTGKI IEKQILLTLE NQISKKDSTL NTMISNIIEE KVRNYLNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Riboflavin biosynthesis in Saccharomyces cerevisiae. Cloning, characterization, and expression of the RIB5 gene encoding riboflavin synthase." Santos M.A., Garcia-Ramirez J.J., Revuelta J.L. J. Biol. Chem. 270:437-444(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae." Doignon F., Biteau N., Crouzet M., Aigle M. Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z21621 Genomic DNA. Translation: CAA79745.1. X70529 Genomic DNA. Translation: CAA49920.1. Z36125 Genomic DNA. Translation: CAA85219.1. BK006936 Genomic DNA. Translation: DAA07373.1.
PIR	S34072.
RefSeq	NP_009815.1. NM_001178604.1.
3D structure databases
ProteinModelPortal	P38145.
SMR	P38145. Positions 1-205.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	32952. 20 interactions.
MINT	MINT-4477838.
STRING	4932.YBR256C.
Proteomic databases
PaxDb	P38145.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR256C; YBR256C; YBR256C.
GeneID	852559.
KEGG	sce:YBR256C.
Organism-specific databases
CYGD	YBR256c.
SGD	S000000460. RIB5.
Phylogenomic databases
eggNOG	COG0307.
HOGENOM	HOG000151757.
KO	K00793.
OMA	VVNIERA.
OrthoDB	EOG793BMH.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER3O-75. YEAST:MONOMER3O-75.
UniPathway	UPA00275; UER00405.
Gene expression databases
Genevestigator	P38145.
Family and domain databases
Gene3D	2.40.30.20. 2 hits.
InterPro	IPR023366. ATPase_asu-like. IPR001783. Lumazine-bd. IPR026017. Lumazine-bd_dom. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
PANTHER	PTHR21098. PTHR21098. 1 hit.
Pfam	PF00677. Lum_binding. 2 hits. [Graphical view]
PIRSF	PIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAM	SSF63380. SSF63380. 2 hits.
TIGRFAMs	TIGR00187. ribE. 1 hit.
PROSITE	PS51177. LUMAZINE_BIND. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	971664.
PRO	P38145.

Entry information

Entry name

RISA_YEAST

Accession

Primary (citable) accession number: P38145
Secondary accession number(s): D6VQQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	March 19, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program