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P38145

- RISA_YEAST

UniProt

P38145 - RISA_YEAST

Protein

Riboflavin synthase

Gene

RIB5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.1 Publication

    Catalytic activityi

    2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.

    Pathwayi

    GO - Molecular functioni

    1. oxidoreductase activity Source: InterPro
    2. riboflavin synthase activity Source: SGD

    GO - Biological processi

    1. riboflavin biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Riboflavin biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER3O-75.
    YEAST:MONOMER3O-75.
    UniPathwayiUPA00275; UER00405.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Riboflavin synthase (EC:2.5.1.9)
    Short name:
    RS
    Gene namesi
    Name:RIB5
    Ordered Locus Names:YBR256C
    ORF Names:YBR1724
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR256c.
    SGDiS000000460. RIB5.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 238238Riboflavin synthasePRO_0000068174Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei95 – 951Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38145.
    PaxDbiP38145.

    Expressioni

    Gene expression databases

    GenevestigatoriP38145.

    Interactioni

    Subunit structurei

    Homotrimer.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-2083267,EBI-2083267
    ARO8P530901EBI-2083267,EBI-2042933
    IPP1P008171EBI-2083267,EBI-9338
    RNR1P215241EBI-2083267,EBI-15234
    VAS1P07806-11EBI-2083267,EBI-1009942From a different organism.

    Protein-protein interaction databases

    BioGridi32952. 20 interactions.
    MINTiMINT-4477838.
    STRINGi4932.YBR256C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38145.
    SMRiP38145. Positions 1-205.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati1 – 103103Lumazine-binding 1Add
    BLAST
    Repeati104 – 205102Lumazine-binding 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni87 – 915Lumazine bindingCurated
    Regioni189 – 1935Lumazine bindingCurated

    Sequence similaritiesi

    Contains 2 lumazine-binding repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0307.
    HOGENOMiHOG000151757.
    KOiK00793.
    OMAiEEFTADV.
    OrthoDBiEOG793BMH.

    Family and domain databases

    Gene3Di2.40.30.20. 2 hits.
    InterProiIPR023366. ATPase_asu-like.
    IPR001783. Lumazine-bd.
    IPR026017. Lumazine-bd_dom.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PANTHERiPTHR21098. PTHR21098. 1 hit.
    PfamiPF00677. Lum_binding. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000498. Riboflavin_syn_A. 1 hit.
    SUPFAMiSSF63380. SSF63380. 2 hits.
    TIGRFAMsiTIGR00187. ribE. 1 hit.
    PROSITEiPS51177. LUMAZINE_BIND. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38145-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFTGIVECMG TVLENNPYDD SESGGQGVSI TIGNAGSILT DCHVGDSIAV    50
    NGVCLTVTEF NNDSFKVGIS PETIKRSNVA SWIQGTQVNL ERAVSQDVRF 100
    GGHYVQGHVD TVANIVSRRP EGNSIIFGFQ LRDQEYFKYI VEKGFICIDG 150
    TSLTIIKVDP LSQGGAFYIS MIKHTQDNVI MPLKKIGDEV NIEVDLTGKI 200
    IEKQILLTLE NQISKKDSTL NTMISNIIEE KVRNYLNK 238
    Length:238
    Mass (Da):26,196
    Last modified:October 1, 1994 - v1
    Checksum:iEA57EAD542D46897
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21621 Genomic DNA. Translation: CAA79745.1.
    X70529 Genomic DNA. Translation: CAA49920.1.
    Z36125 Genomic DNA. Translation: CAA85219.1.
    BK006936 Genomic DNA. Translation: DAA07373.1.
    PIRiS34072.
    RefSeqiNP_009815.1. NM_001178604.1.

    Genome annotation databases

    EnsemblFungiiYBR256C; YBR256C; YBR256C.
    GeneIDi852559.
    KEGGisce:YBR256C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21621 Genomic DNA. Translation: CAA79745.1 .
    X70529 Genomic DNA. Translation: CAA49920.1 .
    Z36125 Genomic DNA. Translation: CAA85219.1 .
    BK006936 Genomic DNA. Translation: DAA07373.1 .
    PIRi S34072.
    RefSeqi NP_009815.1. NM_001178604.1.

    3D structure databases

    ProteinModelPortali P38145.
    SMRi P38145. Positions 1-205.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32952. 20 interactions.
    MINTi MINT-4477838.
    STRINGi 4932.YBR256C.

    Proteomic databases

    MaxQBi P38145.
    PaxDbi P38145.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR256C ; YBR256C ; YBR256C .
    GeneIDi 852559.
    KEGGi sce:YBR256C.

    Organism-specific databases

    CYGDi YBR256c.
    SGDi S000000460. RIB5.

    Phylogenomic databases

    eggNOGi COG0307.
    HOGENOMi HOG000151757.
    KOi K00793.
    OMAi EEFTADV.
    OrthoDBi EOG793BMH.

    Enzyme and pathway databases

    UniPathwayi UPA00275 ; UER00405 .
    BioCyci MetaCyc:MONOMER3O-75.
    YEAST:MONOMER3O-75.

    Miscellaneous databases

    NextBioi 971664.
    PROi P38145.

    Gene expression databases

    Genevestigatori P38145.

    Family and domain databases

    Gene3Di 2.40.30.20. 2 hits.
    InterProi IPR023366. ATPase_asu-like.
    IPR001783. Lumazine-bd.
    IPR026017. Lumazine-bd_dom.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    PANTHERi PTHR21098. PTHR21098. 1 hit.
    Pfami PF00677. Lum_binding. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000498. Riboflavin_syn_A. 1 hit.
    SUPFAMi SSF63380. SSF63380. 2 hits.
    TIGRFAMsi TIGR00187. ribE. 1 hit.
    PROSITEi PS51177. LUMAZINE_BIND. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Riboflavin biosynthesis in Saccharomyces cerevisiae. Cloning, characterization, and expression of the RIB5 gene encoding riboflavin synthase."
      Santos M.A., Garcia-Ramirez J.J., Revuelta J.L.
      J. Biol. Chem. 270:437-444(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "The complete sequence of a 19,482 bp segment located on the right arm of chromosome II from Saccharomyces cerevisiae."
      Doignon F., Biteau N., Crouzet M., Aigle M.
      Yeast 9:189-199(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRISA_YEAST
    AccessioniPrimary (citable) accession number: P38145
    Secondary accession number(s): D6VQQ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3