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Protein

ISWI chromatin-remodeling complex ATPase ISW1

Gene

ISW1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic component of ISW1-type complexes, which act by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. They are involved in coordinating transcriptional repression, activation and elongation phases. The ISW1A complex represses gene expression at initiation through specific positioning of a promoter proximal dinucleosome. The ISW1B complex acts within coding regions to control the amount of RNA polymerase II released into productive elongation and to coordinate elongation with termination and pre-mRNA processing.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 228ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • helicase activity Source: UniProtKB-KW
  • nucleosome binding Source: InterPro
  • rDNA binding Source: SGD
  • transcription regulatory region DNA binding Source: SGD

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: InterPro
  • chromatin modification Source: UniProtKB-KW
  • chromatin remodeling Source: SGD
  • DNA-templated transcription, elongation Source: SGD
  • heterochromatin maintenance involved in chromatin silencing Source: SGD
  • negative regulation of histone exchange Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • regulation of chromatin organization Source: SGD
  • regulation of nucleosome density Source: SGD
  • regulation of transcriptional start site selection at RNA polymerase II promoter Source: SGD
  • termination of RNA polymerase II transcription Source: SGD
  • termination of RNA polymerase I transcription Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Helicase, Hydrolase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29174-MONOMER.
ReactomeiR-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-5696400. Dual Incision in GG-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
ISWI chromatin-remodeling complex ATPase ISW1 (EC:3.6.4.-)
Gene namesi
Name:ISW1
Ordered Locus Names:YBR245C
ORF Names:YBR1633
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR245C.
SGDiS000000449. ISW1.

Subcellular locationi

GO - Cellular componenti

  • Isw1a complex Source: SGD
  • Isw1b complex Source: SGD
  • nucleolar chromatin Source: SGD
  • NURF complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi227K → A: Abolishes ATPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743301 – 1129ISWI chromatin-remodeling complex ATPase ISW1Add BLAST1129

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei694PhosphothreonineCombined sources1
Modified residuei846PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38144.
PRIDEiP38144.

PTM databases

iPTMnetiP38144.

Interactioni

Subunit structurei

Component of the ISW1A complex, which at least consists of ISW1 and IOC3. Component of the ISW1B complex, which at least consists of ISW1, IOC2 and IOC4.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IOC2Q120726EBI-21087,EBI-30191
IOC3P435969EBI-21087,EBI-22944

Protein-protein interaction databases

BioGridi32940. 171 interactors.
DIPiDIP-6601N.
IntActiP38144. 70 interactors.
MINTiMINT-614918.

Structurei

Secondary structure

11129
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi814 – 827Combined sources14
Turni828 – 830Combined sources3
Helixi835 – 838Combined sources4
Turni839 – 841Combined sources3
Turni848 – 850Combined sources3
Helixi851 – 862Combined sources12
Beta strandi866 – 868Combined sources3
Helixi870 – 882Combined sources13
Helixi889 – 902Combined sources14
Helixi907 – 911Combined sources5
Beta strandi917 – 919Combined sources3
Helixi920 – 932Combined sources13
Turni941 – 943Combined sources3
Helixi944 – 968Combined sources25
Helixi974 – 977Combined sources4
Helixi994 – 1007Combined sources14
Helixi1015 – 1024Combined sources10
Helixi1027 – 1029Combined sources3
Helixi1032 – 1035Combined sources4
Helixi1039 – 1054Combined sources16
Turni1055 – 1058Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y9YX-ray3.25A763-1129[»]
2Y9ZX-ray3.60A763-1129[»]
ProteinModelPortaliP38144.
SMRiP38144.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38144.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini208 – 373Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST166
Domaini506 – 657Helicase C-terminalPROSITE-ProRule annotationAdd BLAST152
Domaini882 – 935SANT 1PROSITE-ProRule annotationAdd BLAST54
Domaini988 – 1052SANT 2PROSITE-ProRule annotationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi324 – 327DEAH box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 2 SANT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00860000134152.
HOGENOMiHOG000192862.
InParanoidiP38144.
KOiK11654.
OMAiERWCPSI.
OrthoDBiEOG092C1YH4.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR009057. Homeodomain-like.
IPR029915. ISWI.
IPR015194. ISWI_HAND-dom.
IPR027417. P-loop_NTPase.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR015195. SLIDE.
IPR000330. SNF2_N.
[Graphical view]
PANTHERiPTHR10799:SF691. PTHR10799:SF691. 2 hits.
PfamiPF09110. HAND. 1 hit.
PF00271. Helicase_C. 1 hit.
PF09111. SLIDE. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF101224. SSF101224. 1 hit.
SSF46689. SSF46689. 2 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51293. SANT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38144-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYMLAIANF HFFKFYTRMR KKHENNSCNE KDKDENLFKI ILAIFLQEKK
60 70 80 90 100
KYDCISSGSI MTASEEYLEN LKPFQVGLPP HDPESNKKRY LLKDANGKKF
110 120 130 140 150
DLEGTTKRFE HLLSLSGLFK HFIESKAAKD PKFRQVLDVL EENKANGKGK
160 170 180 190 200
GKHQDVRRRK TEHEEDAELL KEEDSDDDES IEFQFRESPA YVNGQLRPYQ
210 220 230 240 250
IQGVNWLVSL HKNKIAGILA DEMGLGKTLQ TISFLGYLRY IEKIPGPFLV
260 270 280 290 300
IAPKSTLNNW LREINRWTPD VNAFILQGDK EERAELIQKK LLGCDFDVVI
310 320 330 340 350
ASYEIIIREK SPLKKINWEY IIIDEAHRIK NEESMLSQVL REFTSRNRLL
360 370 380 390 400
ITGTPLQNNL HELWALLNFL LPDIFSDAQD FDDWFSSEST EEDQDKIVKQ
410 420 430 440 450
LHTVLQPFLL RRIKSDVETS LLPKKELNLY VGMSSMQKKW YKKILEKDLD
460 470 480 490 500
AVNGSNGSKE SKTRLLNIMM QLRKCCNHPY LFDGAEPGPP YTTDEHLVYN
510 520 530 540 550
AAKLQVLDKL LKKLKEEGSR VLIFSQMSRL LDILEDYCYF RNYEYCRIDG
560 570 580 590 600
STAHEDRIQA IDDYNAPDSK KFVFLLTTRA GGLGINLTSA DVVVLYDSDW
610 620 630 640 650
NPQADLQAMD RAHRIGQKKQ VKVFRLVTDN SVEEKILERA TQKLRLDQLV
660 670 680 690 700
IQQNRTSLKK KENKADSKDA LLSMIQHGAA DVFKSGTSTG SAGTPEPGSG
710 720 730 740 750
EKGDDIDLDE LLLKSENKTK SLNAKYETLG LDDLQKFNQD SAYEWNGQDF
760 770 780 790 800
KKKIQRDIIS PLLLNPTKRE RKENYSIDNY YKDVLNTGRS STPSHPRMPK
810 820 830 840 850
PHVFHSHQLQ PPQLKVLYEK ERMWTAKKTG YVPTMDDVKA AYGDISDEEE
860 870 880 890 900
KKQKLELLKL SVNNSQPLTE EEEKMKADWE SEGFTNWNKL EFRKFITVSG
910 920 930 940 950
KYGRNSIQAI ARELAPGKTL EEVRAYAKAF WSNIERIEDY EKYLKIIENE
960 970 980 990 1000
EEKIKRVKMQ QEALRRKLSE YKNPFFDLKL KHPPSSNNKR TYSEEEDRFI
1010 1020 1030 1040 1050
LLMLFKYGLD RDDVYELVRD EIRDCPLFEL DFYFRSRTPV ELARRGNTLL
1060 1070 1080 1090 1100
QCLEKEFNAG IVLDDATKDR MKKEDENGKR IREEFADQTA NEKENVDGVE
1110 1120
SKKAKIEDTS NVGTEQLVAE KIPENETTH
Length:1,129
Mass (Da):131,102
Last modified:November 1, 1997 - v2
Checksum:i2CF86BAF553E6695
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36114 Genomic DNA. Translation: CAA85208.1.
BK006936 Genomic DNA. Translation: DAA07361.1.
PIRiS46122.
RefSeqiNP_009804.1. NM_001178593.1.

Genome annotation databases

EnsemblFungiiYBR245C; YBR245C; YBR245C.
GeneIDi852547.
KEGGisce:YBR245C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36114 Genomic DNA. Translation: CAA85208.1.
BK006936 Genomic DNA. Translation: DAA07361.1.
PIRiS46122.
RefSeqiNP_009804.1. NM_001178593.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y9YX-ray3.25A763-1129[»]
2Y9ZX-ray3.60A763-1129[»]
ProteinModelPortaliP38144.
SMRiP38144.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32940. 171 interactors.
DIPiDIP-6601N.
IntActiP38144. 70 interactors.
MINTiMINT-614918.

PTM databases

iPTMnetiP38144.

Proteomic databases

MaxQBiP38144.
PRIDEiP38144.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR245C; YBR245C; YBR245C.
GeneIDi852547.
KEGGisce:YBR245C.

Organism-specific databases

EuPathDBiFungiDB:YBR245C.
SGDiS000000449. ISW1.

Phylogenomic databases

GeneTreeiENSGT00860000134152.
HOGENOMiHOG000192862.
InParanoidiP38144.
KOiK11654.
OMAiERWCPSI.
OrthoDBiEOG092C1YH4.

Enzyme and pathway databases

BioCyciYEAST:G3O-29174-MONOMER.
ReactomeiR-SCE-5696395. Formation of Incision Complex in GG-NER.
R-SCE-5696400. Dual Incision in GG-NER.

Miscellaneous databases

EvolutionaryTraceiP38144.
PROiP38144.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR009057. Homeodomain-like.
IPR029915. ISWI.
IPR015194. ISWI_HAND-dom.
IPR027417. P-loop_NTPase.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR015195. SLIDE.
IPR000330. SNF2_N.
[Graphical view]
PANTHERiPTHR10799:SF691. PTHR10799:SF691. 2 hits.
PfamiPF09110. HAND. 1 hit.
PF00271. Helicase_C. 1 hit.
PF09111. SLIDE. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF101224. SSF101224. 1 hit.
SSF46689. SSF46689. 2 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51293. SANT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISW1_YEAST
AccessioniPrimary (citable) accession number: P38144
Secondary accession number(s): D6VQP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 161 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.