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Protein

ISWI chromatin-remodeling complex ATPase ISW1

Gene

ISW1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic component of ISW1-type complexes, which act by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. They are involved in coordinating transcriptional repression, activation and elongation phases. The ISW1A complex represses gene expression at initiation through specific positioning of a promoter proximal dinucleosome. The ISW1B complex acts within coding regions to control the amount of RNA polymerase II released into productive elongation and to coordinate elongation with termination and pre-mRNA processing.4 Publications

Miscellaneous

Present with 1500 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 228ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • helicase activity Source: UniProtKB-KW
  • nucleosome binding Source: InterPro
  • rDNA binding Source: SGD
  • transcription regulatory region DNA binding Source: SGD

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: InterPro
  • chromatin remodeling Source: SGD
  • DNA-templated transcription, elongation Source: SGD
  • heterochromatin maintenance involved in chromatin silencing Source: SGD
  • negative regulation of histone exchange Source: SGD
  • positive regulation of transcription by RNA polymerase II Source: SGD
  • regulation of chromatin organization Source: SGD
  • regulation of nucleosome density Source: SGD
  • regulation of transcriptional start site selection at RNA polymerase II promoter Source: SGD
  • sister chromatid cohesion Source: SGD
  • termination of RNA polymerase II transcription Source: SGD
  • termination of RNA polymerase I transcription Source: SGD

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Helicase, Hydrolase, Repressor
Biological processTranscription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29174-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ISWI chromatin-remodeling complex ATPase ISW1 (EC:3.6.4.-)
Gene namesi
Name:ISW1
Ordered Locus Names:YBR245C
ORF Names:YBR1633
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR245C
SGDiS000000449 ISW1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi227K → A: Abolishes ATPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743301 – 1129ISWI chromatin-remodeling complex ATPase ISW1Add BLAST1129

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei694PhosphothreonineCombined sources1
Modified residuei846PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38144
PaxDbiP38144
PRIDEiP38144

PTM databases

iPTMnetiP38144

Interactioni

Subunit structurei

Component of the ISW1A complex, which at least consists of ISW1 and IOC3. Component of the ISW1B complex, which at least consists of ISW1, IOC2 and IOC4.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32940, 1857 interactors
DIPiDIP-6601N
IntActiP38144, 71 interactors
MINTiP38144
STRINGi4932.YBR245C

Structurei

Secondary structure

11129
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi814 – 827Combined sources14
Turni828 – 830Combined sources3
Helixi835 – 838Combined sources4
Turni839 – 841Combined sources3
Turni848 – 850Combined sources3
Helixi851 – 862Combined sources12
Beta strandi866 – 868Combined sources3
Helixi870 – 882Combined sources13
Helixi889 – 902Combined sources14
Helixi907 – 911Combined sources5
Beta strandi917 – 919Combined sources3
Helixi920 – 932Combined sources13
Turni941 – 943Combined sources3
Helixi944 – 968Combined sources25
Helixi974 – 977Combined sources4
Helixi994 – 1007Combined sources14
Helixi1015 – 1024Combined sources10
Helixi1027 – 1029Combined sources3
Helixi1032 – 1035Combined sources4
Helixi1039 – 1054Combined sources16
Turni1055 – 1058Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Y9YX-ray3.25A763-1129[»]
2Y9ZX-ray3.60A763-1129[»]
ProteinModelPortaliP38144
SMRiP38144
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38144

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini208 – 373Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST166
Domaini506 – 657Helicase C-terminalPROSITE-ProRule annotationAdd BLAST152
Domaini882 – 935SANT 1PROSITE-ProRule annotationAdd BLAST54
Domaini988 – 1052SANT 2PROSITE-ProRule annotationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi324 – 327DEAH box4

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00910000145423
HOGENOMiHOG000192862
InParanoidiP38144
KOiK11654
OMAiLEDYCHW
OrthoDBiEOG092C1YH4

Family and domain databases

CDDicd00079 HELICc, 1 hit
cd00167 SANT, 1 hit
Gene3Di1.10.1040.30, 1 hit
3.40.50.10810, 1 hit
InterProiView protein in InterPro
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR009057 Homeobox-like_sf
IPR015194 ISWI_HAND-dom
IPR036306 ISWI_HAND-dom_sf
IPR027417 P-loop_NTPase
IPR001005 SANT/Myb
IPR017884 SANT_dom
IPR015195 SLIDE
IPR038718 SNF2-like_sf
IPR000330 SNF2_N
PfamiView protein in Pfam
PF09110 HAND, 1 hit
PF00271 Helicase_C, 1 hit
PF09111 SLIDE, 1 hit
PF00176 SNF2_N, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SM00717 SANT, 2 hits
SUPFAMiSSF101224 SSF101224, 1 hit
SSF46689 SSF46689, 2 hits
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51293 SANT, 1 hit

Sequencei

Sequence statusi: Complete.

P38144-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYMLAIANF HFFKFYTRMR KKHENNSCNE KDKDENLFKI ILAIFLQEKK
60 70 80 90 100
KYDCISSGSI MTASEEYLEN LKPFQVGLPP HDPESNKKRY LLKDANGKKF
110 120 130 140 150
DLEGTTKRFE HLLSLSGLFK HFIESKAAKD PKFRQVLDVL EENKANGKGK
160 170 180 190 200
GKHQDVRRRK TEHEEDAELL KEEDSDDDES IEFQFRESPA YVNGQLRPYQ
210 220 230 240 250
IQGVNWLVSL HKNKIAGILA DEMGLGKTLQ TISFLGYLRY IEKIPGPFLV
260 270 280 290 300
IAPKSTLNNW LREINRWTPD VNAFILQGDK EERAELIQKK LLGCDFDVVI
310 320 330 340 350
ASYEIIIREK SPLKKINWEY IIIDEAHRIK NEESMLSQVL REFTSRNRLL
360 370 380 390 400
ITGTPLQNNL HELWALLNFL LPDIFSDAQD FDDWFSSEST EEDQDKIVKQ
410 420 430 440 450
LHTVLQPFLL RRIKSDVETS LLPKKELNLY VGMSSMQKKW YKKILEKDLD
460 470 480 490 500
AVNGSNGSKE SKTRLLNIMM QLRKCCNHPY LFDGAEPGPP YTTDEHLVYN
510 520 530 540 550
AAKLQVLDKL LKKLKEEGSR VLIFSQMSRL LDILEDYCYF RNYEYCRIDG
560 570 580 590 600
STAHEDRIQA IDDYNAPDSK KFVFLLTTRA GGLGINLTSA DVVVLYDSDW
610 620 630 640 650
NPQADLQAMD RAHRIGQKKQ VKVFRLVTDN SVEEKILERA TQKLRLDQLV
660 670 680 690 700
IQQNRTSLKK KENKADSKDA LLSMIQHGAA DVFKSGTSTG SAGTPEPGSG
710 720 730 740 750
EKGDDIDLDE LLLKSENKTK SLNAKYETLG LDDLQKFNQD SAYEWNGQDF
760 770 780 790 800
KKKIQRDIIS PLLLNPTKRE RKENYSIDNY YKDVLNTGRS STPSHPRMPK
810 820 830 840 850
PHVFHSHQLQ PPQLKVLYEK ERMWTAKKTG YVPTMDDVKA AYGDISDEEE
860 870 880 890 900
KKQKLELLKL SVNNSQPLTE EEEKMKADWE SEGFTNWNKL EFRKFITVSG
910 920 930 940 950
KYGRNSIQAI ARELAPGKTL EEVRAYAKAF WSNIERIEDY EKYLKIIENE
960 970 980 990 1000
EEKIKRVKMQ QEALRRKLSE YKNPFFDLKL KHPPSSNNKR TYSEEEDRFI
1010 1020 1030 1040 1050
LLMLFKYGLD RDDVYELVRD EIRDCPLFEL DFYFRSRTPV ELARRGNTLL
1060 1070 1080 1090 1100
QCLEKEFNAG IVLDDATKDR MKKEDENGKR IREEFADQTA NEKENVDGVE
1110 1120
SKKAKIEDTS NVGTEQLVAE KIPENETTH
Length:1,129
Mass (Da):131,102
Last modified:November 1, 1997 - v2
Checksum:i2CF86BAF553E6695
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36114 Genomic DNA Translation: CAA85208.1
BK006936 Genomic DNA Translation: DAA07361.1
PIRiS46122
RefSeqiNP_009804.1, NM_001178593.1

Genome annotation databases

EnsemblFungiiYBR245C; YBR245C; YBR245C
GeneIDi852547
KEGGisce:YBR245C

Similar proteinsi

Entry informationi

Entry nameiISW1_YEAST
AccessioniPrimary (citable) accession number: P38144
Secondary accession number(s): D6VQP1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 176 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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