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Protein

ISWI chromatin-remodeling complex ATPase ISW1

Gene

ISW1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic component of ISW1-type complexes, which act by remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. They are involved in coordinating transcriptional repression, activation and elongation phases. The ISW1A complex represses gene expression at initiation through specific positioning of a promoter proximal dinucleosome. The ISW1B complex acts within coding regions to control the amount of RNA polymerase II released into productive elongation and to coordinate elongation with termination and pre-mRNA processing.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi221 – 2288ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • helicase activity Source: UniProtKB-KW
  • rDNA binding Source: SGD
  • transcription regulatory region DNA binding Source: SGD

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: InterPro
  • chromatin remodeling Source: SGD
  • DNA-templated transcription, elongation Source: SGD
  • heterochromatin maintenance involved in chromatin silencing Source: SGD
  • negative regulation of histone exchange Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • regulation of chromatin organization Source: SGD
  • regulation of nucleosome density Source: SGD
  • regulation of transcriptional start site selection at RNA polymerase II promoter Source: SGD
  • termination of RNA polymerase II transcription Source: SGD
  • termination of RNA polymerase I transcription Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Helicase, Hydrolase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29174-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ISWI chromatin-remodeling complex ATPase ISW1 (EC:3.6.4.-)
Gene namesi
Name:ISW1
Ordered Locus Names:YBR245C
ORF Names:YBR1633
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR245C.
SGDiS000000449. ISW1.

Subcellular locationi

GO - Cellular componenti

  • Isw1a complex Source: SGD
  • Isw1b complex Source: SGD
  • nucleolar chromatin Source: SGD
  • NURF complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi227 – 2271K → A: Abolishes ATPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11291129ISWI chromatin-remodeling complex ATPase ISW1PRO_0000074330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei694 – 6941PhosphothreonineCombined sources
Modified residuei846 – 8461PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38144.

PTM databases

iPTMnetiP38144.

Interactioni

Subunit structurei

Component of the ISW1A complex, which at least consists of ISW1 and IOC3. Component of the ISW1B complex, which at least consists of ISW1, IOC2 and IOC4.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IOC2Q120726EBI-21087,EBI-30191
IOC3P435969EBI-21087,EBI-22944

Protein-protein interaction databases

BioGridi32940. 171 interactions.
DIPiDIP-6601N.
IntActiP38144. 70 interactions.
MINTiMINT-614918.

Structurei

Secondary structure

1
1129
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi814 – 82714Combined sources
Turni828 – 8303Combined sources
Helixi835 – 8384Combined sources
Turni839 – 8413Combined sources
Turni848 – 8503Combined sources
Helixi851 – 86212Combined sources
Beta strandi866 – 8683Combined sources
Helixi870 – 88213Combined sources
Helixi889 – 90214Combined sources
Helixi907 – 9115Combined sources
Beta strandi917 – 9193Combined sources
Helixi920 – 93213Combined sources
Turni941 – 9433Combined sources
Helixi944 – 96825Combined sources
Helixi974 – 9774Combined sources
Helixi994 – 100714Combined sources
Helixi1015 – 102410Combined sources
Helixi1027 – 10293Combined sources
Helixi1032 – 10354Combined sources
Helixi1039 – 105416Combined sources
Turni1055 – 10584Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y9YX-ray3.25A763-1129[»]
2Y9ZX-ray3.60A763-1129[»]
ProteinModelPortaliP38144.
SMRiP38144. Positions 187-736, 789-1075.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38144.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini208 – 373166Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini506 – 657152Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini882 – 93554SANT 1PROSITE-ProRule annotationAdd
BLAST
Domaini988 – 105265SANT 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi324 – 3274DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 2 SANT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00830000128349.
HOGENOMiHOG000192862.
InParanoidiP38144.
KOiK11654.
OMAiERWCPSI.
OrthoDBiEOG092C1YH4.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR009057. Homeodomain-like.
IPR029915. ISWI.
IPR015194. ISWI_HAND-dom.
IPR027417. P-loop_NTPase.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR015195. SLIDE.
IPR000330. SNF2_N.
[Graphical view]
PANTHERiPTHR10799:SF691. PTHR10799:SF691. 2 hits.
PfamiPF09110. HAND. 1 hit.
PF00271. Helicase_C. 1 hit.
PF09111. SLIDE. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF101224. SSF101224. 1 hit.
SSF46689. SSF46689. 2 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51293. SANT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38144-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYMLAIANF HFFKFYTRMR KKHENNSCNE KDKDENLFKI ILAIFLQEKK
60 70 80 90 100
KYDCISSGSI MTASEEYLEN LKPFQVGLPP HDPESNKKRY LLKDANGKKF
110 120 130 140 150
DLEGTTKRFE HLLSLSGLFK HFIESKAAKD PKFRQVLDVL EENKANGKGK
160 170 180 190 200
GKHQDVRRRK TEHEEDAELL KEEDSDDDES IEFQFRESPA YVNGQLRPYQ
210 220 230 240 250
IQGVNWLVSL HKNKIAGILA DEMGLGKTLQ TISFLGYLRY IEKIPGPFLV
260 270 280 290 300
IAPKSTLNNW LREINRWTPD VNAFILQGDK EERAELIQKK LLGCDFDVVI
310 320 330 340 350
ASYEIIIREK SPLKKINWEY IIIDEAHRIK NEESMLSQVL REFTSRNRLL
360 370 380 390 400
ITGTPLQNNL HELWALLNFL LPDIFSDAQD FDDWFSSEST EEDQDKIVKQ
410 420 430 440 450
LHTVLQPFLL RRIKSDVETS LLPKKELNLY VGMSSMQKKW YKKILEKDLD
460 470 480 490 500
AVNGSNGSKE SKTRLLNIMM QLRKCCNHPY LFDGAEPGPP YTTDEHLVYN
510 520 530 540 550
AAKLQVLDKL LKKLKEEGSR VLIFSQMSRL LDILEDYCYF RNYEYCRIDG
560 570 580 590 600
STAHEDRIQA IDDYNAPDSK KFVFLLTTRA GGLGINLTSA DVVVLYDSDW
610 620 630 640 650
NPQADLQAMD RAHRIGQKKQ VKVFRLVTDN SVEEKILERA TQKLRLDQLV
660 670 680 690 700
IQQNRTSLKK KENKADSKDA LLSMIQHGAA DVFKSGTSTG SAGTPEPGSG
710 720 730 740 750
EKGDDIDLDE LLLKSENKTK SLNAKYETLG LDDLQKFNQD SAYEWNGQDF
760 770 780 790 800
KKKIQRDIIS PLLLNPTKRE RKENYSIDNY YKDVLNTGRS STPSHPRMPK
810 820 830 840 850
PHVFHSHQLQ PPQLKVLYEK ERMWTAKKTG YVPTMDDVKA AYGDISDEEE
860 870 880 890 900
KKQKLELLKL SVNNSQPLTE EEEKMKADWE SEGFTNWNKL EFRKFITVSG
910 920 930 940 950
KYGRNSIQAI ARELAPGKTL EEVRAYAKAF WSNIERIEDY EKYLKIIENE
960 970 980 990 1000
EEKIKRVKMQ QEALRRKLSE YKNPFFDLKL KHPPSSNNKR TYSEEEDRFI
1010 1020 1030 1040 1050
LLMLFKYGLD RDDVYELVRD EIRDCPLFEL DFYFRSRTPV ELARRGNTLL
1060 1070 1080 1090 1100
QCLEKEFNAG IVLDDATKDR MKKEDENGKR IREEFADQTA NEKENVDGVE
1110 1120
SKKAKIEDTS NVGTEQLVAE KIPENETTH
Length:1,129
Mass (Da):131,102
Last modified:November 1, 1997 - v2
Checksum:i2CF86BAF553E6695
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36114 Genomic DNA. Translation: CAA85208.1.
BK006936 Genomic DNA. Translation: DAA07361.1.
PIRiS46122.
RefSeqiNP_009804.1. NM_001178593.1.

Genome annotation databases

EnsemblFungiiYBR245C; YBR245C; YBR245C.
GeneIDi852547.
KEGGisce:YBR245C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36114 Genomic DNA. Translation: CAA85208.1.
BK006936 Genomic DNA. Translation: DAA07361.1.
PIRiS46122.
RefSeqiNP_009804.1. NM_001178593.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y9YX-ray3.25A763-1129[»]
2Y9ZX-ray3.60A763-1129[»]
ProteinModelPortaliP38144.
SMRiP38144. Positions 187-736, 789-1075.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32940. 171 interactions.
DIPiDIP-6601N.
IntActiP38144. 70 interactions.
MINTiMINT-614918.

PTM databases

iPTMnetiP38144.

Proteomic databases

MaxQBiP38144.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR245C; YBR245C; YBR245C.
GeneIDi852547.
KEGGisce:YBR245C.

Organism-specific databases

EuPathDBiFungiDB:YBR245C.
SGDiS000000449. ISW1.

Phylogenomic databases

GeneTreeiENSGT00830000128349.
HOGENOMiHOG000192862.
InParanoidiP38144.
KOiK11654.
OMAiERWCPSI.
OrthoDBiEOG092C1YH4.

Enzyme and pathway databases

BioCyciYEAST:G3O-29174-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP38144.
PROiP38144.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR009057. Homeodomain-like.
IPR029915. ISWI.
IPR015194. ISWI_HAND-dom.
IPR027417. P-loop_NTPase.
IPR001005. SANT/Myb.
IPR017884. SANT_dom.
IPR015195. SLIDE.
IPR000330. SNF2_N.
[Graphical view]
PANTHERiPTHR10799:SF691. PTHR10799:SF691. 2 hits.
PfamiPF09110. HAND. 1 hit.
PF00271. Helicase_C. 1 hit.
PF09111. SLIDE. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00717. SANT. 2 hits.
[Graphical view]
SUPFAMiSSF101224. SSF101224. 1 hit.
SSF46689. SSF46689. 2 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51293. SANT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISW1_YEAST
AccessioniPrimary (citable) accession number: P38144
Secondary accession number(s): D6VQP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.