ID   THI2_YEAST              Reviewed;         450 AA.
AC   P38141; D6VQN6;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   27-MAR-2024, entry version 161.
DE   RecName: Full=Thiamine biosynthesis regulatory protein;
GN   Name=THI2; Synonyms=PHO6; OrderedLocusNames=YBR240C; ORFNames=YBR1624;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=S288c / GRF88;
RA   Nishimura H., Nosaka K., Kaneko Y., Iwashima A.;
RL   Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA   Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA   Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA   Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA   Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA   Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA   Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA   Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA   Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA   Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA   Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA   Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA   Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA   Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA   Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA   Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA   Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA   Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA   Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA   Mewes H.-W., Kleine K.;
RT   "Complete DNA sequence of yeast chromosome II.";
RL   EMBO J. 13:5795-5809(1994).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
CC   -!- FUNCTION: Positive regulator of thiamine biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
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DR   EMBL; D32073; BAA22093.1; -; Genomic_DNA.
DR   EMBL; Z36109; CAA85203.1; -; Genomic_DNA.
DR   EMBL; BK006936; DAA07356.1; -; Genomic_DNA.
DR   PIR; S46117; S46117.
DR   RefSeq; NP_009799.3; NM_001178588.3.
DR   AlphaFoldDB; P38141; -.
DR   SMR; P38141; -.
DR   BioGRID; 32935; 35.
DR   DIP; DIP-5362N; -.
DR   IntAct; P38141; 1.
DR   MINT; P38141; -.
DR   STRING; 4932.YBR240C; -.
DR   PaxDb; 4932-YBR240C; -.
DR   EnsemblFungi; YBR240C_mRNA; YBR240C; YBR240C.
DR   GeneID; 852542; -.
DR   KEGG; sce:YBR240C; -.
DR   AGR; SGD:S000000444; -.
DR   SGD; S000000444; THI2.
DR   VEuPathDB; FungiDB:YBR240C; -.
DR   eggNOG; ENOG502QU1Y; Eukaryota.
DR   HOGENOM; CLU_050082_0_0_1; -.
DR   InParanoid; P38141; -.
DR   OMA; CWACRLK; -.
DR   OrthoDB; 2025637at2759; -.
DR   BioCyc; YEAST:G3O-29171-MONOMER; -.
DR   BioGRID-ORCS; 852542; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P38141; -.
DR   Proteomes; UP000002311; Chromosome II.
DR   RNAct; P38141; Protein.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:SGD.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0090180; P:positive regulation of thiamine biosynthetic process; IMP:SGD.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:SGD.
DR   GO; GO:0009228; P:thiamine biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; Zn(2)-C6 fungal-type DNA-binding domain; 1.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   InterPro; IPR001138; Zn2Cys6_DnaBD.
DR   PANTHER; PTHR31069; OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED; 1.
DR   PANTHER; PTHR31069:SF34; THIAMINE BIOSYNTHESIS REGULATORY PROTEIN; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; Zn2/Cys6 DNA-binding domain; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   4: Predicted;
KW   Activator; DNA-binding; Metal-binding; Nucleus; Reference proteome;
KW   Thiamine biosynthesis; Transcription; Transcription regulation; Zinc.
FT   CHAIN           1..450
FT                   /note="Thiamine biosynthesis regulatory protein"
FT                   /id="PRO_0000114984"
FT   DNA_BIND        30..57
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..23
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          210..234
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   450 AA;  51823 MW;  1A8B1627EC66FD93 CRC64;
     MVNSKRQQRS KKVASSSKVP PTKGRTFTGC WACRFKKRRC DENRPICSLC AKHGDNCSYD
     IRLMWLEENI YKVRKHSLIS SLQARKSKSK PLCQKISKSR FKQMTHFRQL SPPTSDCEDS
     VHEASKETTL PNDNTFTISV RRLKIYNNAV ASVFGSMTNR DYTQKRIDKK LDELLNMVEN
     DISVVNLNCS KHGPYSVFRA NPAAVTSALT DQLPSPGHSM SSAEETTTAA LSSPPEDSTS
     LIDIIQGKIF GILWFNCYGN MILNRQEYTT WFINKMRNSL TTEFIRFLGK IIDDPDINMA
     SCLFKECIAR WSCVDWQSIA ITMLVIIHGY TCPNLTKLLR VWFLQQKLLR FSMYPLVNFI
     INNTQDLDVL YHCNGLLGNA DLFEDPYQDE LTSELHVLVT ERLVNSWKDT ILQQLCSCQD
     TTLSCSQLRY WQLQLKCNQQ FYKDVYAMQD
//