P38141 (THI2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiamine biosynthesis regulatory protein

Gene names

Name:	THI2
Synonyms:	PHO6
Ordered Locus Names:	YBR240C
ORF Names:	YBR1624

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Positive regulator of thiamine biosynthesis.
Subcellular location	Nucleus Probable.
Sequence similarities	Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Ontologies

Keywords
Biological process	Thiamine biosynthesis Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	thiamine biosynthetic process Inferred from mutant phenotype PubMed 1551422. Source: SGD
Cellular_component	nucleus Inferred from direct assay PubMed 16194233. Source: SGD
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription Inferred from genetic interaction PubMed 16194233. Source: SGD sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Thiamine biosynthesis regulatory protein		PRO_0000114984
Regions
	DNA binding	30 – 57	28	Zn(2)-C6 fungal-type

Sequences

	Sequence		Length	Mass (Da)
	P38141 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 1A8B1627EC66FD93	FASTA	450	51,823
	10 20 30 40 50 60 MVNSKRQQRS KKVASSSKVP PTKGRTFTGC WACRFKKRRC DENRPICSLC AKHGDNCSYD 70 80 90 100 110 120 IRLMWLEENI YKVRKHSLIS SLQARKSKSK PLCQKISKSR FKQMTHFRQL SPPTSDCEDS 130 140 150 160 170 180 VHEASKETTL PNDNTFTISV RRLKIYNNAV ASVFGSMTNR DYTQKRIDKK LDELLNMVEN 190 200 210 220 230 240 DISVVNLNCS KHGPYSVFRA NPAAVTSALT DQLPSPGHSM SSAEETTTAA LSSPPEDSTS 250 260 270 280 290 300 LIDIIQGKIF GILWFNCYGN MILNRQEYTT WFINKMRNSL TTEFIRFLGK IIDDPDINMA 310 320 330 340 350 360 SCLFKECIAR WSCVDWQSIA ITMLVIIHGY TCPNLTKLLR VWFLQQKLLR FSMYPLVNFI 370 380 390 400 410 420 INNTQDLDVL YHCNGLLGNA DLFEDPYQDE LTSELHVLVT ERLVNSWKDT ILQQLCSCQD 430 440 450 TTLSCSQLRY WQLQLKCNQQ FYKDVYAMQD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Nishimura H., Nosaka K., Kaneko Y., Iwashima A. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D32073 Genomic DNA. Translation: BAA22093.1. Z36109 Genomic DNA. Translation: CAA85203.1. BK006936 Genomic DNA. Translation: DAA07356.1.
PIR	S46117.
RefSeq	NP_009799.3. NM_001178588.3.
3D structure databases
ProteinModelPortal	P38141.
SMR	P38141. Positions 26-60.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5362N.
MINT	MINT-556210.
STRING	4932.YBR240C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR240C; YBR240C; YBR240C.
GeneID	852542.
KEGG	sce:YBR240C. sce:YBR244W.
Organism-specific databases
CYGD	YBR240c.
SGD	S000000444. THI2.
Phylogenomic databases
eggNOG	NOG68468.
KO	K00432.
OrthoDB	EOG4PVS6R.
Gene expression databases
Genevestigator	P38141.
GermOnline	YBR240C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	4.10.240.10. 1 hit.
InterPro	IPR001138. Zn2-C6_fun-type_DNA-bd. [Graphical view]
Pfam	PF00172. Zn_clus. 1 hit. [Graphical view]
SMART	SM00066. GAL4. 1 hit. [Graphical view]
SUPFAM	SSF57701. Fungi_TrN. 1 hit.
PROSITE	PS00463. ZN2_CY6_FUNGAL_1. 1 hit. PS50048. ZN2_CY6_FUNGAL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971613.

Entry information

Entry name

THI2_YEAST

Accession

Primary (citable) accession number: P38141
Secondary accession number(s): D6VQN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program