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Protein

Thiamine biosynthesis regulatory protein

Gene

THI2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Positive regulator of thiamine biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi30 – 5728Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  2. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. positive regulation of thiamine biosynthetic process Source: SGD
  2. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  3. thiamine biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Thiamine biosynthesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29171-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine biosynthesis regulatory protein
Gene namesi
Name:THI2
Synonyms:PHO6
Ordered Locus Names:YBR240C
ORF Names:YBR1624
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome II

Organism-specific databases

CYGDiYBR240c.
SGDiS000000444. THI2.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450Thiamine biosynthesis regulatory proteinPRO_0000114984Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP38141.

Interactioni

Protein-protein interaction databases

BioGridi32935. 12 interactions.
DIPiDIP-5362N.
IntActiP38141. 1 interaction.
MINTiMINT-556210.
STRINGi4932.YBR240C.

Structurei

3D structure databases

ProteinModelPortaliP38141.
SMRiP38141. Positions 26-59.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG68468.
InParanoidiP38141.
OrthoDBiEOG72ZCQ3.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38141-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNSKRQQRS KKVASSSKVP PTKGRTFTGC WACRFKKRRC DENRPICSLC
60 70 80 90 100
AKHGDNCSYD IRLMWLEENI YKVRKHSLIS SLQARKSKSK PLCQKISKSR
110 120 130 140 150
FKQMTHFRQL SPPTSDCEDS VHEASKETTL PNDNTFTISV RRLKIYNNAV
160 170 180 190 200
ASVFGSMTNR DYTQKRIDKK LDELLNMVEN DISVVNLNCS KHGPYSVFRA
210 220 230 240 250
NPAAVTSALT DQLPSPGHSM SSAEETTTAA LSSPPEDSTS LIDIIQGKIF
260 270 280 290 300
GILWFNCYGN MILNRQEYTT WFINKMRNSL TTEFIRFLGK IIDDPDINMA
310 320 330 340 350
SCLFKECIAR WSCVDWQSIA ITMLVIIHGY TCPNLTKLLR VWFLQQKLLR
360 370 380 390 400
FSMYPLVNFI INNTQDLDVL YHCNGLLGNA DLFEDPYQDE LTSELHVLVT
410 420 430 440 450
ERLVNSWKDT ILQQLCSCQD TTLSCSQLRY WQLQLKCNQQ FYKDVYAMQD
Length:450
Mass (Da):51,823
Last modified:October 1, 1994 - v1
Checksum:i1A8B1627EC66FD93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32073 Genomic DNA. Translation: BAA22093.1.
Z36109 Genomic DNA. Translation: CAA85203.1.
BK006936 Genomic DNA. Translation: DAA07356.1.
PIRiS46117.
RefSeqiNP_009799.3. NM_001178588.3.

Genome annotation databases

EnsemblFungiiYBR240C; YBR240C; YBR240C.
GeneIDi852542.
KEGGisce:YBR240C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D32073 Genomic DNA. Translation: BAA22093.1.
Z36109 Genomic DNA. Translation: CAA85203.1.
BK006936 Genomic DNA. Translation: DAA07356.1.
PIRiS46117.
RefSeqiNP_009799.3. NM_001178588.3.

3D structure databases

ProteinModelPortaliP38141.
SMRiP38141. Positions 26-59.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32935. 12 interactions.
DIPiDIP-5362N.
IntActiP38141. 1 interaction.
MINTiMINT-556210.
STRINGi4932.YBR240C.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR240C; YBR240C; YBR240C.
GeneIDi852542.
KEGGisce:YBR240C.

Organism-specific databases

CYGDiYBR240c.
SGDiS000000444. THI2.

Phylogenomic databases

eggNOGiNOG68468.
InParanoidiP38141.
OrthoDBiEOG72ZCQ3.

Enzyme and pathway databases

BioCyciYEAST:G3O-29171-MONOMER.

Miscellaneous databases

NextBioi971613.

Gene expression databases

GenevestigatoriP38141.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Nishimura H., Nosaka K., Kaneko Y., Iwashima A.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiTHI2_YEAST
AccessioniPrimary (citable) accession number: P38141
Secondary accession number(s): D6VQN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: January 7, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.