P38140 (ERT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription activator of gluconeogenesis ERT1

Alternative name(s):
Ethanol regulator of translation 1

Gene names

Name:	ERT1
Ordered Locus Names:	YBR239C
ORF Names:	YBR1622

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor which regulates nonfermentable carbon utilization. Activator of gluconeogenetic genes like PCK1. Involved in restriction of Ty1 transposition. Ref.6 Ref.7
Subcellular location	Cytoplasm. Nucleus Ref.3.
Disruption phenotype	Increases the periodicity of transcriptional and metabolic oscillation. Ref.5
Miscellaneous	Present with 85 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the ERT1/acuK family. Contains 1 PAS (PER-ARNT-SIM) domain. Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Ontologies

Keywords
Biological process	Gluconeogenesis Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay Ref.3. Source: SGD nucleus Inferred from direct assay Ref.3. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from direct assay. Source: SGD sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro signal transducer activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LSB3	P43603	2	EBI-21048,EBI-22980
RVS167	P39743	2	EBI-21048,EBI-14500
YSC84	P32793	2	EBI-21048,EBI-24460

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Transcription activator of gluconeogenesis ERT1		PRO_0000114993
Regions
	Domain	408 – 480	73	PAS
	DNA binding	40 – 68	29	Zn(2)-C6 fungal-type

Sequences

	Sequence		Length	Mass (Da)
	P38140 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 6C89B70CBCCA93D8	FASTA	529	60,311
	10 20 30 40 50 60 MCTPDENDYK TSTDPDTSAN TNHTLEKKKR KKRKNTNVAC VNCSRLHVSC EAKRPCLRCI 70 80 90 100 110 120 SKGLTATCVD APRKKSKYLA GIPNRELPMN IQPDLPPRKI MIPIYNNSSN SSLNVNNMGE 130 140 150 160 170 180 QQKFTSPQHI VHKAKFLSNA ADSEYSILSN IIYQDTLSNK IPIDILYSNT NSTSNSTIGN 190 200 210 220 230 240 SSNNSPTGTN TSPEETEMEK IRQLYSEQRA NIPPHPYPSS NQNVYSILLG PNSAKIVASQ 250 260 270 280 290 300 VNLFANHFPL VPVDSADNSL NFKRLLPRDP SEKSSQINWD SSINQYYLNS ETVTFPELAI 310 320 330 340 350 360 PLKRRKNHLV SVSLESCSPD AANIKSNVEW EHSLRYSTPM EIYTSINAPF SHTPGFHHLL 370 380 390 400 410 420 VYLKHRFNQQ DLVKMCRSIA EFRPIFIACS VTLTEEDMIF MEQCYQRTLL EYVKFIAQIG 430 440 450 460 470 480 TPTCIWRRNG QISYVNEEFE ILCGWTREEL LNKMTFIVEI MDDESVRDYF KTLSKVAYRD 490 500 510 520 FRGSEKMKVC RLLSPIKGKI IHCCCMWTLK RDVSGLPLMI LGNFMPILN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Evolution of the clock from yeast to man by period-doubling folds in the cellular oscillator." Klevecz R.R., Li C.M. Cold Spring Harb. Symp. Quant. Biol. 72:421-429(2007) [PubMed: 18419300] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[6]	"Chromatin-associated genes protect the yeast genome from Ty1 insertional mutagenesis." Nyswaner K.M., Checkley M.A., Yi M., Stephens R.M., Garfinkel D.J. Genetics 178:197-214(2008) [PubMed: 18202368] [Abstract] Cited for: FUNCTION.
[7]	"Transcriptional regulation of nonfermentable carbon utilization in budding yeast." Turcotte B., Liang X.B., Robert F., Soontorngun N. FEMS Yeast Res. 10:2-13(2010) [PubMed: 19686338] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36108 Genomic DNA. Translation: CAA85202.1. BK006936 Genomic DNA. Translation: DAA07355.1.
PIR	S46116.
RefSeq	NP_009798.1. NM_001178587.1.
3D structure databases
ProteinModelPortal	P38140.
SMR	P38140. Positions 38-70.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4432N.
IntAct	P38140. 6 interactions.
MINT	MINT-498032.
STRING	P38140.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR239C; YBR239C; YBR239C.
GeneID	852541.
KEGG	sce:YBR239C.
NMPDR	fig|4932.3.peg.513.
Organism-specific databases
CYGD	YBR239c.
SGD	S000000443. ERT1.
Phylogenomic databases
eggNOG	fuNOG05082.
GeneTree	EFGT00050000003808.
HOGENOM	HBG397916.
OMA	DIPMLIV.
OrthoDB	EOG44BF9G.
Gene expression databases
ArrayExpress	P38140.
Genevestigator	P38140.
GermOnline	YBR239C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000014. PAS. IPR013767. PAS_fold. IPR001138. Zn2-C6_fun-type_DNA-bd. [Graphical view]
Gene3D	G3DSA:4.10.240.10. Zn2-C6_fun-type_DNA-bd. 1 hit.
Pfam	PF00989. PAS. 1 hit. PF00172. Zn_clus. 1 hit. [Graphical view]
SMART	SM00066. GAL4. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
SUPFAM	SSF57701. Fungi_TrN. 1 hit.
TIGRFAMs	TIGR00229. Sensory_box. 1 hit.
PROSITE	PS50112. PAS. 1 hit. PS00463. ZN2_CY6_FUNGAL_1. 1 hit. PS50048. ZN2_CY6_FUNGAL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971610.

Entry information

Entry name

ERT1_YEAST

Accession

Primary (citable) accession number: P38140
Secondary accession number(s): D6VQN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program