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P38138

- GLU2A_YEAST

UniProt

P38138 - GLU2A_YEAST

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Protein

Glucosidase 2 subunit alpha

Gene
ROT2, GLS2, YBR229C, YBR1526
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic subunit of glucosidase 2, which cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature glycoproteins.2 Publications

Catalytic activityi

Hydrolysis of terminal (1->3)-alpha-D-glucosidic links in (1->3)-alpha-D-glucans.

Enzyme regulationi

Inhibited by glucose, maltose and nigerose, and by the antibiotic deoxynojirimycin.

pH dependencei

Optimum pH is 5.8-6.8.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei537 – 5371Nucleophile By similarity
Active sitei540 – 5401 By similarity
Active sitei614 – 6141Proton donor By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glucan 1,3-alpha-glucosidase activity Source: SGD

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. fungal-type cell wall biogenesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciYEAST:YBR229C-MONOMER.
UniPathwayiUPA00957.

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosidase 2 subunit alpha (EC:3.2.1.84)
Alternative name(s):
Alpha-glucosidase II subunit alpha
Glucosidase II subunit alpha
Reversal of TOR2 lethality protein 2
Gene namesi
Name:ROT2
Synonyms:GLS2
Ordered Locus Names:YBR229C
ORF Names:YBR1526
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR229c.
SGDiS000000433. ROT2.

Subcellular locationi

Endoplasmic reticulum 1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. endoplasmic reticulum lumen Source: SGD
  3. glucosidase II complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed predictionAdd
BLAST
Chaini23 – 954932Glucosidase 2 subunit alphaPRO_0000185370Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi114 – 1141N-linked (GlcNAc...) Reviewed prediction
Glycosylationi126 – 1261N-linked (GlcNAc...) Reviewed prediction
Glycosylationi142 – 1421N-linked (GlcNAc...) Reviewed prediction
Glycosylationi173 – 1731N-linked (GlcNAc...) Reviewed prediction
Glycosylationi345 – 3451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi783 – 7831N-linked (GlcNAc...) Reviewed prediction
Glycosylationi791 – 7911N-linked (GlcNAc...) Reviewed prediction
Glycosylationi867 – 8671N-linked (GlcNAc...) Reviewed prediction
Glycosylationi880 – 8801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi907 – 9071N-linked (GlcNAc...) Reviewed prediction
Glycosylationi941 – 9411N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP38138.
PaxDbiP38138.
PeptideAtlasiP38138.
PRIDEiP38138.

Expressioni

Gene expression databases

GenevestigatoriP38138.

Interactioni

Subunit structurei

Heterodimer of a catalytic subunit alpha (ROT2) and a subunit beta (GTB1).

Binary interactionsi

WithEntry#Exp.IntActNotes
GTB1Q049241EBI-21021,EBI-37493

Protein-protein interaction databases

BioGridi32924. 115 interactions.
DIPiDIP-5754N.
IntActiP38138. 3 interactions.
MINTiMINT-1364745.
STRINGi4932.YBR229C.

Structurei

3D structure databases

ProteinModelPortaliP38138.
SMRiP38138. Positions 290-897.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1501.
GeneTreeiENSGT00740000115152.
HOGENOMiHOG000115864.
KOiK05546.
OMAiAIDDQLY.
OrthoDBiEOG7G7KXG.

Family and domain databases

InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 3 hits.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38138-1 [UniParc]FASTAAdd to Basket

« Hide

MVLLKWLVCQ LVFFTAFSHA FTDYLLKKCA QSGFCHRNRV YAENIAKSHH    50
CYYKVDAESI AHDPLENVLH ATIIKTIPRL EGDDIAVQFP FSLSFLQDHS 100
VRFTINEKER MPTNSSGLLI SSQRFNETWK YAFDKKFQEE ANRTSIPQFH 150
FLKQKQTVNS FWSKISSFLS LSNSTADTFH LRNGDVSVEI FAEPFQLKVY 200
WQNALKLIVN EQNFLNIEHH RTKQENFAHV LPEETTFNMF KDNFLYSKHD 250
SMPLGPESVA LDFSFMGSTN VYGIPEHATS LRLMDTSGGK EPYRLFNVDV 300
FEYNIGTSQP MYGSIPFMFS SSSTSIFWVN AADTWVDIKY DTSKNKTMTH 350
WISENGVIDV VMSLGPDIPT IIDKFTDLTG RPFLPPISSI GYHQCRWNYN 400
DEMDVLTVDS QMDAHMIPYD FIWLDLEYTN DKKYFTWKQH SFPNPKRLLS 450
KLKKLGRNLV VLIDPHLKKD YEISDRVINE NVAVKDHNGN DYVGHCWPGN 500
SIWIDTISKY GQKIWKSFFE RFMDLPADLT NLFIWNDMNE PSIFDGPETT 550
APKDLIHDNY IEERSVHNIY GLSVHEATYD AIKSIYSPSD KRPFLLTRAF 600
FAGSQRTAAT WTGDNVANWD YLKISIPMVL SNNIAGMPFI GADIAGFAED 650
PTPELIARWY QAGLWYPFFR AHAHIDTKRR EPYLFNEPLK SIVRDIIQLR 700
YFLLPTLYTM FHKSSVTGFP IMNPMFIEHP EFAELYHIDN QFYWSNSGLL 750
VKPVTEPGQS ETEMVFPPGI FYEFASLHSF INNGTDLIEK NISAPLDKIP 800
LFIEGGHIIT MKDKYRRSSM LMKNDPYVIV IAPDTEGRAV GDLYVDDGET 850
FGYQRGEYVE TQFIFENNTL KNVRSHIPEN LTGIHHNTLR NTNIEKIIIA 900
KNNLQHNITL KDSIKVKKNG EESSLPTRSS YENDNKITIL NLSLDITEDW 950
EVIF 954
Length:954
Mass (Da):110,266
Last modified:October 1, 1994 - v1
Checksum:i92E93572F04FB009
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z36098 Genomic DNA. Translation: CAA85192.1.
BK006936 Genomic DNA. Translation: DAA07344.1.
PIRiS46105.
RefSeqiNP_009788.3. NM_001178577.3.

Genome annotation databases

EnsemblFungiiYBR229C; YBR229C; YBR229C.
GeneIDi852530.
KEGGisce:YBR229C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z36098 Genomic DNA. Translation: CAA85192.1 .
BK006936 Genomic DNA. Translation: DAA07344.1 .
PIRi S46105.
RefSeqi NP_009788.3. NM_001178577.3.

3D structure databases

ProteinModelPortali P38138.
SMRi P38138. Positions 290-897.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32924. 115 interactions.
DIPi DIP-5754N.
IntActi P38138. 3 interactions.
MINTi MINT-1364745.
STRINGi 4932.YBR229C.

Protein family/group databases

CAZyi GH31. Glycoside Hydrolase Family 31.

Proteomic databases

MaxQBi P38138.
PaxDbi P38138.
PeptideAtlasi P38138.
PRIDEi P38138.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR229C ; YBR229C ; YBR229C .
GeneIDi 852530.
KEGGi sce:YBR229C.

Organism-specific databases

CYGDi YBR229c.
SGDi S000000433. ROT2.

Phylogenomic databases

eggNOGi COG1501.
GeneTreei ENSGT00740000115152.
HOGENOMi HOG000115864.
KOi K05546.
OMAi AIDDQLY.
OrthoDBi EOG7G7KXG.

Enzyme and pathway databases

UniPathwayi UPA00957 .
BioCyci YEAST:YBR229C-MONOMER.

Miscellaneous databases

NextBioi 971580.

Gene expression databases

Genevestigatori P38138.

Family and domain databases

InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 3 hits.
PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Inhibition of N-linked complex oligosaccharide formation by 1-deoxynojirimycin, an inhibitor of processing glucosidases."
    Saunier B., Kilker R.D. Jr., Tkacz J.S., Quaroni A., Herscovics A.
    J. Biol. Chem. 257:14155-14161(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
  4. "Endoplasmic reticulum glucosidase II is composed of a catalytic subunit, conserved from yeast to mammals, and a tightly bound noncatalytic HDEL-containing subunit."
    Trombetta E.S., Simons J.F., Helenius A.
    J. Biol. Chem. 271:27509-27516(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Yeast GTB1 encodes a subunit of glucosidase II required for glycoprotein processing in the endoplasmic reticulum."
    Wilkinson B.M., Purswani J., Stirling C.J.
    J. Biol. Chem. 281:6325-6333(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GTB1, SUBCELLULAR LOCATION, GLYCOSYLATION.

Entry informationi

Entry nameiGLU2A_YEAST
AccessioniPrimary (citable) accession number: P38138
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 3, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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