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Protein

Glucosidase 2 subunit alpha

Gene

ROT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of glucosidase 2, which cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature glycoproteins.2 Publications

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Hydrolysis of terminal (1->3)-alpha-D-glucosidic links in (1->3)-alpha-D-glucans.

Enzyme regulationi

Inhibited by glucose, maltose and nigerose, and by the antibiotic deoxynojirimycin.

pH dependencei

Optimum pH is 5.8-6.8.1 Publication

Pathwayi: N-glycan metabolism

This protein is involved in the pathway N-glycan metabolism, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway N-glycan metabolism and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei537NucleophilePROSITE-ProRule annotation1
Active sitei540By similarity1
Active sitei614Proton donorBy similarity1

GO - Molecular functioni

  • carbohydrate binding Source: InterPro
  • glucan 1,3-alpha-glucosidase activity Source: SGD

GO - Biological processi

  • fungal-type cell wall beta-glucan biosynthetic process Source: SGD
  • N-glycan processing Source: SGD

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciMetaCyc:YBR229C-MONOMER
YEAST:YBR229C-MONOMER
ReactomeiR-SCE-189085 Digestion of dietary carbohydrate
R-SCE-6798695 Neutrophil degranulation
R-SCE-70221 Glycogen breakdown (glycogenolysis)
R-SCE-901042 Calnexin/calreticulin cycle
UniPathwayiUPA00957

Protein family/group databases

CAZyiGH31 Glycoside Hydrolase Family 31

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosidase 2 subunit alpha (EC:3.2.1.84)
Alternative name(s):
Alpha-glucosidase II subunit alpha
Glucosidase II subunit alpha
Reversal of TOR2 lethality protein 2
Gene namesi
Name:ROT2
Synonyms:GLS2
Ordered Locus Names:YBR229C
ORF Names:YBR1526
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR229C
SGDiS000000433 ROT2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000018537023 – 954Glucosidase 2 subunit alphaAdd BLAST932

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi126N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi142N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi173N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi345N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi783N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi791N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi867N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi880N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi907N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi941N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP38138
PaxDbiP38138
PRIDEiP38138

Interactioni

Subunit structurei

Heterodimer of a catalytic subunit alpha (ROT2) and a subunit beta (GTB1).

Binary interactionsi

WithEntry#Exp.IntActNotes
GTB1Q049242EBI-21021,EBI-37493

Protein-protein interaction databases

BioGridi32924, 160 interactors
DIPiDIP-5754N
IntActiP38138, 3 interactors
MINTiP38138
STRINGi4932.YBR229C

Structurei

3D structure databases

ProteinModelPortaliP38138
SMRiP38138
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00760000119229
HOGENOMiHOG000115864
InParanoidiP38138
KOiK05546
OMAiTVHQPLW
OrthoDBiEOG092C0B8A

Family and domain databases

Gene3Di2.60.40.1180, 2 hits
InterProiView protein in InterPro
IPR033403 DUF5110
IPR011013 Gal_mutarotase_sf_dom
IPR000322 Glyco_hydro_31
IPR030458 Glyco_hydro_31_AS
IPR025887 Glyco_hydro_31_N_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF17137 DUF5110, 1 hit
PF13802 Gal_mutarotas_2, 1 hit
PF01055 Glyco_hydro_31, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF74650 SSF74650, 3 hits
PROSITEiView protein in PROSITE
PS00129 GLYCOSYL_HYDROL_F31_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38138-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLLKWLVCQ LVFFTAFSHA FTDYLLKKCA QSGFCHRNRV YAENIAKSHH
60 70 80 90 100
CYYKVDAESI AHDPLENVLH ATIIKTIPRL EGDDIAVQFP FSLSFLQDHS
110 120 130 140 150
VRFTINEKER MPTNSSGLLI SSQRFNETWK YAFDKKFQEE ANRTSIPQFH
160 170 180 190 200
FLKQKQTVNS FWSKISSFLS LSNSTADTFH LRNGDVSVEI FAEPFQLKVY
210 220 230 240 250
WQNALKLIVN EQNFLNIEHH RTKQENFAHV LPEETTFNMF KDNFLYSKHD
260 270 280 290 300
SMPLGPESVA LDFSFMGSTN VYGIPEHATS LRLMDTSGGK EPYRLFNVDV
310 320 330 340 350
FEYNIGTSQP MYGSIPFMFS SSSTSIFWVN AADTWVDIKY DTSKNKTMTH
360 370 380 390 400
WISENGVIDV VMSLGPDIPT IIDKFTDLTG RPFLPPISSI GYHQCRWNYN
410 420 430 440 450
DEMDVLTVDS QMDAHMIPYD FIWLDLEYTN DKKYFTWKQH SFPNPKRLLS
460 470 480 490 500
KLKKLGRNLV VLIDPHLKKD YEISDRVINE NVAVKDHNGN DYVGHCWPGN
510 520 530 540 550
SIWIDTISKY GQKIWKSFFE RFMDLPADLT NLFIWNDMNE PSIFDGPETT
560 570 580 590 600
APKDLIHDNY IEERSVHNIY GLSVHEATYD AIKSIYSPSD KRPFLLTRAF
610 620 630 640 650
FAGSQRTAAT WTGDNVANWD YLKISIPMVL SNNIAGMPFI GADIAGFAED
660 670 680 690 700
PTPELIARWY QAGLWYPFFR AHAHIDTKRR EPYLFNEPLK SIVRDIIQLR
710 720 730 740 750
YFLLPTLYTM FHKSSVTGFP IMNPMFIEHP EFAELYHIDN QFYWSNSGLL
760 770 780 790 800
VKPVTEPGQS ETEMVFPPGI FYEFASLHSF INNGTDLIEK NISAPLDKIP
810 820 830 840 850
LFIEGGHIIT MKDKYRRSSM LMKNDPYVIV IAPDTEGRAV GDLYVDDGET
860 870 880 890 900
FGYQRGEYVE TQFIFENNTL KNVRSHIPEN LTGIHHNTLR NTNIEKIIIA
910 920 930 940 950
KNNLQHNITL KDSIKVKKNG EESSLPTRSS YENDNKITIL NLSLDITEDW

EVIF
Length:954
Mass (Da):110,266
Last modified:October 1, 1994 - v1
Checksum:i92E93572F04FB009
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36098 Genomic DNA Translation: CAA85192.1
BK006936 Genomic DNA Translation: DAA07344.1
PIRiS46105
RefSeqiNP_009788.3, NM_001178577.3

Genome annotation databases

EnsemblFungiiYBR229C; YBR229C; YBR229C
GeneIDi852530
KEGGisce:YBR229C

Similar proteinsi

Entry informationi

Entry nameiGLU2A_YEAST
AccessioniPrimary (citable) accession number: P38138
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

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