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Protein

Short-chain-fatty-acid--CoA ligase

Gene

fadK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the esterification, concomitant with transport, of exogenous fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It has a low activity on medium or long chain fatty acids and is maximally active on C6:0 and C8:0 substrates (PubMed:15213221). FadK is not expressed under aerobic growth conditions and it could functionally replace FadD under anaerobic conditions (PubMed:15213221).1 Publication

Catalytic activityi

ATP + a short-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.1 Publication

pH dependencei

Optimum pH is 7.7 - 8.2.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi231 – 242ATPBy similarityAdd BLAST12

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • medium-chain fatty acid-CoA ligase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12357-MONOMER.
ECOL316407:JW5910-MONOMER.
MetaCyc:EG12357-MONOMER.

Chemistry databases

SwissLipidsiSLP:000000973.

Names & Taxonomyi

Protein namesi
Recommended name:
Short-chain-fatty-acid--CoA ligase (EC:6.2.1.-1 Publication)
Alternative name(s):
Acyl-CoA synthetase
Gene namesi
Name:fadK1 Publication
Synonyms:ydiD
Ordered Locus Names:b1701, JW5910
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12357. fadK.

Subcellular locationi

  • Membrane; Peripheral membrane protein

  • Note: Partially membrane-associated.1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931321 – 566Short-chain-fatty-acid--CoA ligaseAdd BLAST566

Proteomic databases

PaxDbiP38135.
PRIDEiP38135.

Expressioni

Inductioni

Expression independent of FadR (PubMed:12535077). FadK is not expressed under aerobic growth conditions, the levels of anaerobic expression vary with the terminal electron acceptor, with more expression during growth on fumarate than on nitrate (at protein level) (PubMed:15213221).2 Publications

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4260285. 308 interactors.
STRINGi511145.b1701.

Structurei

3D structure databases

ProteinModelPortaliP38135.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CEY. Bacteria.
COG0318. LUCA.
HOGENOMiHOG000230000.
InParanoidiP38135.
KOiK12507.
OMAiGKLMPHV.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38135-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPTGPHLGP DVLFRESNMK VTLTFNEQRR AAYRQQGLWG DASLADYWQQ
60 70 80 90 100
TARAMPDKIA VVDNHGASYT YSALDHAASC LANWMLAKGI ESGDRIAFQL
110 120 130 140 150
PGWCEFTVIY LACLKIGAVS VPLLPSWREA ELVWVLNKCQ AKMFFAPTLF
160 170 180 190 200
KQTRPVDLIL PLQNQLPQLQ QIVGVDKLAP ATSSLSLSQI IADNTSLTTA
210 220 230 240 250
ITTHGDELAA VLFTSGTEGL PKGVMLTHNN ILASERAYCA RLNLTWQDVF
260 270 280 290 300
MMPAPLGHAT GFLHGVTAPF LIGARSVLLD IFTPDACLAL LEQQRCTCML
310 320 330 340 350
GATPFVYDLL NVLEKQPADL SALRFFLCGG TTIPKKVARE CQQRGIKLLS
360 370 380 390 400
VYGSTESSPH AVVNLDDPLS RFMHTDGYAA AGVEIKVVDD ARKTLPPGCE
410 420 430 440 450
GEEASRGPNV FMGYFDEPEL TARALDEEGW YYSGDLCRMD EAGYIKITGR
460 470 480 490 500
KKDIIVRGGE NISSREVEDI LLQHPKIHDA CVVAMSDERL GERSCAYVVL
510 520 530 540 550
KAPHHSLSLE EVVAFFSRKR VAKYKYPEHI VVIEKLPRTT SGKIQKFLLR
560
KDIMRRLTQD VCEEIE
Length:566
Mass (Da):62,759
Last modified:September 7, 2016 - v4
Checksum:i057236D5F7F2FCE7
GO

Sequence cautioni

The sequence AAC74771 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence M69116 differs from that shown. Reason: Frameshift at several positions.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74771.2. Different initiation.
AP009048 Genomic DNA. Translation: BAA15470.2.
M69116 Genomic DNA. No translation available.
PIRiE64928.
RefSeqiNP_416216.4. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74771; AAC74771; b1701.
BAA15470; BAA15470; BAA15470.
GeneIDi946213.
KEGGiecj:JW5910.
eco:b1701.
PATRICi32118708. VBIEscCol129921_1772.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74771.2. Different initiation.
AP009048 Genomic DNA. Translation: BAA15470.2.
M69116 Genomic DNA. No translation available.
PIRiE64928.
RefSeqiNP_416216.4. NC_000913.3.

3D structure databases

ProteinModelPortaliP38135.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260285. 308 interactors.
STRINGi511145.b1701.

Chemistry databases

SwissLipidsiSLP:000000973.

Proteomic databases

PaxDbiP38135.
PRIDEiP38135.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74771; AAC74771; b1701.
BAA15470; BAA15470; BAA15470.
GeneIDi946213.
KEGGiecj:JW5910.
eco:b1701.
PATRICi32118708. VBIEscCol129921_1772.

Organism-specific databases

EchoBASEiEB2260.
EcoGeneiEG12357. fadK.

Phylogenomic databases

eggNOGiENOG4105CEY. Bacteria.
COG0318. LUCA.
HOGENOMiHOG000230000.
InParanoidiP38135.
KOiK12507.
OMAiGKLMPHV.

Enzyme and pathway databases

BioCyciEcoCyc:EG12357-MONOMER.
ECOL316407:JW5910-MONOMER.
MetaCyc:EG12357-MONOMER.

Miscellaneous databases

PROiP38135.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFADK_ECOLI
AccessioniPrimary (citable) accession number: P38135
Secondary accession number(s): P76202, P76902, P76903
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: September 7, 2016
Last modified: November 2, 2016
This is version 122 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Probably starts on Met-1; overexpressed protein starting at Met-19 has lower activity, forms aggregates during reaction and is unstable in storage at -80 degrees Celsius (PubMed:15213221).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.