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Protein

Tyrosine-protein kinase etk

Gene

etk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • protein tyrosine kinase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11826-MONOMER.
ECOL316407:JW0964-MONOMER.
MetaCyc:EG11826-MONOMER.
BRENDAi2.7.10.1. 2026.

Protein family/group databases

TCDBi8.A.3.3.1. the cytoplasmic membrane-periplasmic auxiliary-1 (mpa1) protein with cytoplasmic (c) domain (mpa1-c or mpa1+c) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase etk (EC:2.7.10.-)
Gene namesi
Name:etk
Synonyms:yccC
Ordered Locus Names:b0981, JW0964
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11826. etk.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232CytoplasmicSequence analysisAdd
BLAST
Transmembranei33 – 5321HelicalSequence analysisAdd
BLAST
Topological domaini54 – 424371PeriplasmicSequence analysisAdd
BLAST
Transmembranei425 – 44521HelicalSequence analysisAdd
BLAST
Topological domaini446 – 726281CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 726726Tyrosine-protein kinase etkPRO_0000212349Add
BLAST

Post-translational modificationi

Autophosphorylated. Dephosphorylated by etp.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP38134.
PaxDbiP38134.
PRIDEiP38134.

PTM databases

iPTMnetiP38134.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-562015,EBI-562015

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4261406. 386 interactions.
DIPiDIP-9526N.
IntActiP38134. 2 interactions.
MINTiMINT-6743651.
STRINGi511145.b0981.

Structurei

Secondary structure

1
726
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi457 – 4604Combined sources
Turni461 – 4633Combined sources
Beta strandi466 – 4716Combined sources
Helixi475 – 4784Combined sources
Helixi502 – 5054Combined sources
Helixi510 – 52516Combined sources
Beta strandi533 – 54311Combined sources
Helixi545 – 55814Combined sources
Beta strandi563 – 5675Combined sources
Turni570 – 5723Combined sources
Helixi575 – 5784Combined sources
Beta strandi584 – 5863Combined sources
Helixi587 – 5915Combined sources
Helixi597 – 6004Combined sources
Beta strandi602 – 6043Combined sources
Turni605 – 6084Combined sources
Beta strandi609 – 6124Combined sources
Helixi621 – 6255Combined sources
Helixi628 – 64013Combined sources
Beta strandi642 – 6476Combined sources
Turni651 – 6533Combined sources
Helixi656 – 6605Combined sources
Helixi661 – 6633Combined sources
Beta strandi665 – 6728Combined sources
Turni673 – 6753Combined sources
Helixi680 – 69011Combined sources
Beta strandi698 – 7025Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CIOX-ray2.50A/D452-726[»]
ProteinModelPortaliP38134.
SMRiP38134. Positions 452-715.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38134.

Family & Domainsi

Sequence similaritiesi

Belongs to the etk/wzc family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D75. Bacteria.
COG0489. LUCA.
COG3206. LUCA.
HOGENOMiHOG000153997.
InParanoidiP38134.
KOiK16692.
OMAiSQMIEQK.
OrthoDBiEOG69D3H6.
PhylomeDBiP38134.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom.
IPR005702. EPS_synthesis.
IPR032807. GNVR.
IPR003856. LipoPS_biosynth.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01656. CbiA. 1 hit.
PF13807. GNVR. 1 hit.
PF02706. Wzz. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01007. eps_fam. 1 hit.

Sequencei

Sequence statusi: Complete.

P38134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTKNMNTPP GSTQENEIDL LRLVGELWDH RKFIISVTAL FTLIAVAYSL
60 70 80 90 100
LSTPIYQADT LVQVEQKQGN AILSGLSDMI PNSSPESAPE IQLLQSRMIL
110 120 130 140 150
GKTIAELNLR DIVEQKYFPI VGRGWARLTK EKPGELAISW MHIPQLNGQD
160 170 180 190 200
QQLTLTVGEN GHYTLEGEEF TVNGMVGQRL EKDGVALTIA DIKAKPGTQF
210 220 230 240 250
VLSQRTELEA INALQETFTV SERSKESGML ELTMTGDDPQ LITRILNSIA
260 270 280 290 300
NNYLQQNIAR QAAQDSQSLE FLQRQLPEVR SELDQAEEKL NVYRQQRDSV
310 320 330 340 350
DLNLEAKAVL EQIVNVDNQL NELTFREAEI SQLYKKDHPT YRALLEKRQT
360 370 380 390 400
LEQERKRLNK RVSAMPSTQQ EVLRLSRDVE AGRAVYLQLL NRQQELSISK
410 420 430 440 450
SSAIGNVRII DPAVTQPQPV KPKKALNVVL GFILGLFISV GAVLARAMLR
460 470 480 490 500
RGVEAPEQLE EHGISVYATI PMSEWLDKRT RLRKKNLFSN QQRHRTKNIP
510 520 530 540 550
FLAVDNPADS AVEAVRALRT SLHFAMMETE NNILMITGAT PDSGKTFVSS
560 570 580 590 600
TLAAVIAQSD QKVLFIDADL RRGYSHNLFT VSNEHGLSEY LAGKDELNKV
610 620 630 640 650
IQHFGKGGFD VITRGQVPPN PSELLMRDRM RQLLEWANDH YDLVIVDTPP
660 670 680 690 700
MLAVSDAAVV GRSVGTSLLV ARFGLNTAKE VSLSMQRLEQ AGVNIKGAIL
710 720
NGVIKRASTA YSYGYNYYGY SYSEKE
Length:726
Mass (Da):81,242
Last modified:November 1, 1997 - v2
Checksum:iBAA060F59680DA22
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti726 – 7261E → EEN (PubMed:8905232).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74066.1.
AP009048 Genomic DNA. Translation: BAA35746.1.
M58708 Genomic DNA. No translation available.
PIRiC64839.
RefSeqiNP_415501.1. NC_000913.3.
WP_000208650.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74066; AAC74066; b0981.
BAA35746; BAA35746; BAA35746.
GeneIDi947409.
KEGGiecj:JW0964.
eco:b0981.
PATRICi32117185. VBIEscCol129921_1016.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74066.1.
AP009048 Genomic DNA. Translation: BAA35746.1.
M58708 Genomic DNA. No translation available.
PIRiC64839.
RefSeqiNP_415501.1. NC_000913.3.
WP_000208650.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CIOX-ray2.50A/D452-726[»]
ProteinModelPortaliP38134.
SMRiP38134. Positions 452-715.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261406. 386 interactions.
DIPiDIP-9526N.
IntActiP38134. 2 interactions.
MINTiMINT-6743651.
STRINGi511145.b0981.

Protein family/group databases

TCDBi8.A.3.3.1. the cytoplasmic membrane-periplasmic auxiliary-1 (mpa1) protein with cytoplasmic (c) domain (mpa1-c or mpa1+c) family.

PTM databases

iPTMnetiP38134.

Proteomic databases

EPDiP38134.
PaxDbiP38134.
PRIDEiP38134.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74066; AAC74066; b0981.
BAA35746; BAA35746; BAA35746.
GeneIDi947409.
KEGGiecj:JW0964.
eco:b0981.
PATRICi32117185. VBIEscCol129921_1016.

Organism-specific databases

EchoBASEiEB1773.
EcoGeneiEG11826. etk.

Phylogenomic databases

eggNOGiENOG4105D75. Bacteria.
COG0489. LUCA.
COG3206. LUCA.
HOGENOMiHOG000153997.
InParanoidiP38134.
KOiK16692.
OMAiSQMIEQK.
OrthoDBiEOG69D3H6.
PhylomeDBiP38134.

Enzyme and pathway databases

BioCyciEcoCyc:EG11826-MONOMER.
ECOL316407:JW0964-MONOMER.
MetaCyc:EG11826-MONOMER.
BRENDAi2.7.10.1. 2026.

Miscellaneous databases

EvolutionaryTraceiP38134.
PROiP38134.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom.
IPR005702. EPS_synthesis.
IPR032807. GNVR.
IPR003856. LipoPS_biosynth.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01656. CbiA. 1 hit.
PF13807. GNVR. 1 hit.
PF02706. Wzz. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01007. eps_fam. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The complete nucleotide sequence of the Escherichia coli gene appA reveals significant homology between pH 2.5 acid phosphatase and glucose-1-phosphatase."
    Dassa J., Marck C., Boquet P.L.
    J. Bacteriol. 172:5497-5500(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 628-726.
  5. "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome."
    Borodovsky M., Rudd K.E., Koonin E.V.
    Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  6. "Relationship between exopolysaccharide production and protein-tyrosine phosphorylation in Gram-negative bacteria."
    Vincent C., Duclos B., Grangeasse C., Vaganay E., Riberty M., Cozzone A.J., Doublet P.
    J. Mol. Biol. 304:311-321(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12 / JM109 / ATCC 53323.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiETK_ECOLI
AccessioniPrimary (citable) accession number: P38134
Secondary accession number(s): P75879
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Seems to be expressed only in enteropathogenic E.coli strains; in E.coli K12 / MG1655 and K12 / W3110 this operon is silenced by an IS1D insertion in the promoter region.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.