Tyrosine-protein kinase etk - Escherichia coli (strain K12)

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P38134 (ETK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 120. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase etk
EC=2.7.10.-

Gene names

Name:	etk
Synonyms:	yccC
Ordered Locus Names:	b0981, JW0964

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Cell inner membrane; Multi-pass membrane protein. Note: When the protein is overexpressed.
Post-translational modification	Autophosphorylated. Dephosphorylated by etp.
Sequence similarities	Belongs to the etk/wzc family.
Caution	Seems to be expressed only in enteropathogenic E.coli strains; in E.coli K12 / MG1655 and K12 / W3110 this operon is silenced by an IS1D insertion in the promoter region.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	capsule polysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro peptidyl-tyrosine autophosphorylation Inferred from direct assay PubMed 10369665. Source: EcoCyc regulation of catalytic activity Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from direct assay PubMed 10369665. Source: EcoCyc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW enzyme regulator activity Inferred from electronic annotation. Source: InterPro protein tyrosine kinase activity Inferred from direct assay PubMed 10369665. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 726

726

Tyrosine-protein kinase etk

PRO_0000212349

Regions

Topological domain

1 – 32

Cytoplasmic Potential

Transmembrane

33 – 53

Helical; Potential

Topological domain

54 – 424

371

Periplasmic Potential

Transmembrane

425 – 445

Helical; Potential

Topological domain

446 – 726

281

Cytoplasmic Potential

Experimental info

Sequence conflict

726

E → EEN Ref.1

Secondary structure

726

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P38134 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: BAA060F59680DA22
Show »

FASTA

726

81,242

        10         20         30         40         50         60 
MTTKNMNTPP GSTQENEIDL LRLVGELWDH RKFIISVTAL FTLIAVAYSL LSTPIYQADT 

        70         80         90        100        110        120 
LVQVEQKQGN AILSGLSDMI PNSSPESAPE IQLLQSRMIL GKTIAELNLR DIVEQKYFPI 

       130        140        150        160        170        180 
VGRGWARLTK EKPGELAISW MHIPQLNGQD QQLTLTVGEN GHYTLEGEEF TVNGMVGQRL 

       190        200        210        220        230        240 
EKDGVALTIA DIKAKPGTQF VLSQRTELEA INALQETFTV SERSKESGML ELTMTGDDPQ 

       250        260        270        280        290        300 
LITRILNSIA NNYLQQNIAR QAAQDSQSLE FLQRQLPEVR SELDQAEEKL NVYRQQRDSV 

       310        320        330        340        350        360 
DLNLEAKAVL EQIVNVDNQL NELTFREAEI SQLYKKDHPT YRALLEKRQT LEQERKRLNK 

       370        380        390        400        410        420 
RVSAMPSTQQ EVLRLSRDVE AGRAVYLQLL NRQQELSISK SSAIGNVRII DPAVTQPQPV 

       430        440        450        460        470        480 
KPKKALNVVL GFILGLFISV GAVLARAMLR RGVEAPEQLE EHGISVYATI PMSEWLDKRT 

       490        500        510        520        530        540 
RLRKKNLFSN QQRHRTKNIP FLAVDNPADS AVEAVRALRT SLHFAMMETE NNILMITGAT 

       550        560        570        580        590        600 
PDSGKTFVSS TLAAVIAQSD QKVLFIDADL RRGYSHNLFT VSNEHGLSEY LAGKDELNKV 

       610        620        630        640        650        660 
IQHFGKGGFD VITRGQVPPN PSELLMRDRM RQLLEWANDH YDLVIVDTPP MLAVSDAAVV 

       670        680        690        700        710        720 
GRSVGTSLLV ARFGLNTAKE VSLSMQRLEQ AGVNIKGAIL NGVIKRASTA YSYGYNYYGY 


SYSEKE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete nucleotide sequence of the Escherichia coli gene appA reveals significant homology between pH 2.5 acid phosphatase and glucose-1-phosphatase." Dassa J., Marck C., Boquet P.L. J. Bacteriol. 172:5497-5500(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 628-726.
[5]	"Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Borodovsky M., Rudd K.E., Koonin E.V. Nucleic Acids Res. 22:4756-4767(1994) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[6]	"Relationship between exopolysaccharide production and protein-tyrosine phosphorylation in Gram-negative bacteria." Vincent C., Duclos B., Grangeasse C., Vaganay E., Riberty M., Cozzone A.J., Doublet P. J. Mol. Biol. 304:311-321(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12 / JM109 / ATCC 53323.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00096 Genomic DNA. Translation: AAC74066.1.
AP009048 Genomic DNA. Translation: BAA35746.1.
M58708 Genomic DNA. No translation available.

PIR

C64839.

RefSeq

NP_415501.1. NC_000913.2.
YP_489252.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3CIO	X-ray	2.50	A/D	452-726	[»]

ProteinModelPortal

P38134.

SMR

P38134. Positions 452-715.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-9526N.

IntAct

P38134. 2 interactions.

MINT

MINT-6743651.

STRING

511145.b0981.

Protein family/group databases

TCDB

8.A.3.3.1. cytoplasmic membrane-periplasmic auxiliary-1 (MPA1) protein with cytoplasmic (C) domain (MPA1-C or MPA1+C) family.

PTM databases

PhosSite

P010425.

Proteomic databases

PaxDb

P38134.

PRIDE

P38134.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74066; AAC74066; b0981.
BAA35746; BAA35746; BAA35746.

GeneID

12932412.
947409.

KEGG

ecj:Y75_p0952.
eco:b0981.

PATRIC

32117185. VBIEscCol129921_1016.

Organism-specific databases

EchoBASE

EB1773.

EcoGene

EG11826. etk.

Phylogenomic databases

eggNOG

COG0489.

HOGENOM

HOG000153997.

K16692.

OMA

FDIRQFE.

ProtClustDB

PRK09841.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11826-MONOMER.
ECOL316407:JW0964-MONOMER.
MetaCyc:EG11826-MONOMER.

BRENDA

2.7.10.1. 2026.

Gene expression databases

Genevestigator

P38134.

Family and domain databases

InterPro

IPR025669. AAA_dom.
IPR005702. EPS_synthesis.
IPR003856. LipoPS_biosynth.
[Graphical view]

Pfam

PF13614. AAA_31. 1 hit.
PF02706. Wzz. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01007. eps_fam. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P38134.

Entry information

Entry name

ETK_ECOLI

Accession

Primary (citable) accession number: P38134
Secondary accession number(s): P75879

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 120 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents