P38131 (KTR4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable mannosyltransferase KTR4
EC=2.4.1.-

Gene names

Name:	KTR4
Ordered Locus Names:	YBR199W
ORF Names:	YBR1411

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible glycosyltransferase.
Catalytic activity	Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.
Subcellular location	Membrane; Single-pass type II membrane protein Probable.
Miscellaneous	Present with 5240 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the glycosyltransferase 15 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	mannosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Probable mannosyltransferase KTR4		PRO_0000208245
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	33 – 464	432	Lumenal Potential
	Region	33 – 130	98	Stem region By similarity
	Region	131 – 464	334	Catalytic By similarity
Sites
	Active site	352	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	P38131 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 207CE3E43A3B4991	FASTA	464	54,556
	10 20 30 40 50 60 MRFLSKRILK PVLSVIILIS IAVTVVLYFL TANENYLQAV KDSAKSQYAS LRESYKSITG 70 80 90 100 110 120 KTESADELPD HDAEVLDSIM DRLHEPLYEK DTFDPNEVLA ENKQLYEEFL LQEISEPKVD 130 140 150 160 170 180 NLVRSGDPLA GKAKGTILSL VRNSDLEDII SSIQQLEEEY NKNFGYPYTF LNDEEFTDEF 190 200 210 220 230 240 KDGIKSILPK DRVVEFGTIG PDNWNMPDSI DRERYDQEMD KMSKENIQYA EVESYHNMCR 250 260 270 280 290 300 FYSKEFYHHP LLSKYKYVWR LEPNVNFYCK INYDVFQFMN KNDKIYGFVL NLYDSPQTIE 310 320 330 340 350 360 TLWTSTMDFV EEHPNYLNVN GAFAWLKDNS QNPKNYDYTQ GYSTCHFWTN FEIVDLDFLR 370 380 390 400 410 420 SEPYEKYMQY LEEKGGFYYE RWGDAPVRSL ALALFADKSS IHWFRDIGYH HTPYTNCPTC 430 440 450 460 PADSDRCNGN CVPGKFTPWS DLDNQNCQAT WIRHSMSEEE LEMY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence analysis of an 11.7 kb fragment of yeast chromosome II including BEM1, a new gene of the WD-40 repeat family and a new member of the KRE2/MNT1 family." Mallet L., Bussereau F., Jacquet M. Yeast 10:819-831(1994) [PubMed: 7975899] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z21487 Genomic DNA. Translation: CAA79686.1. Z36068 Genomic DNA. Translation: CAA85161.1. Z36069 Genomic DNA. Translation: CAA85163.1. AY692898 Genomic DNA. Translation: AAT92917.1. BK006936 Genomic DNA. Translation: DAA07315.1.
PIR	S34024.
RefSeq	NP_009758.1. NM_001178547.1.
3D structure databases
ProteinModelPortal	P38131.
SMR	P38131. Positions 116-456.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7838N.
IntAct	P38131. 2 interactions.
MINT	MINT-1365179.
STRING	P38131.
Protein family/group databases
CAZy	GT15. Glycosyltransferase Family 15.
Proteomic databases
PeptideAtlas	P38131.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR199W; YBR199W; YBR199W.
GeneID	852498.
KEGG	sce:YBR199W.
NMPDR	fig|4932.3.peg.468.
Organism-specific databases
CYGD	YBR199w.
SGD	S000000403. KTR4.
Phylogenomic databases
eggNOG	fuNOG07858.
GeneTree	EFGT00050000003570.
HOGENOM	HBG396033.
OMA	HNMCRFY.
OrthoDB	EOG4XH380.
Gene expression databases
ArrayExpress	P38131.
Genevestigator	P38131.
GermOnline	YBR199W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002685. Glyco_trans_15. [Graphical view]
KO	K03854.
Pfam	PF01793. Glyco_transf_15. 1 hit. [Graphical view]
PIRSF	PIRSF018153. Glyco_trans_15. 1 hit.
ProtoNet	Search...
Other
NextBio	971498.

Entry information

Entry name

KTR4_YEAST

Accession

Primary (citable) accession number: P38131
Secondary accession number(s): D6VQJ5, P89506

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program