P38130 (KTR3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable mannosyltransferase KTR3
EC=2.4.1.-

Gene names

Name:	KTR3
Ordered Locus Names:	YBR205W
ORF Names:	YBR1445

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible glycosyltransferase.
Catalytic activity	Transfers an alpha-D-mannosyl residue from GDP-mannose into lipid-linked oligosaccharide, forming an alpha-(1->2)-D-mannosyl-D-mannose linkage.
Subunit structure	Interacts with SVP26. Ref.6
Subcellular location	Membrane; Single-pass type II membrane protein Probable.
Miscellaneous	Present with 704 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the glycosyltransferase 15 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-glycan processing Inferred from genetic interaction PubMed 9182588. Source: SGD protein O-linked glycosylation Inferred from genetic interaction PubMed 9182588. Source: SGD
Cellular_component	fungal-type vacuole membrane Inferred from direct assay PubMed 22842922. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay PubMed 22842922. Source: SGD
Molecular_function	mannosyltransferase activity Inferred from mutant phenotype PubMed 9182588. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RPL4A	P10664	3	EBI-9987,EBI-15390

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Probable mannosyltransferase KTR3		PRO_0000208244
Regions
	Topological domain	1 – 27	27	Cytoplasmic Potential
	Transmembrane	28 – 44	17	Helical; Signal-anchor for type II membrane protein
	Topological domain	45 – 404	360	Lumenal Potential
	Region	45 – 83	39	Stem region By similarity
	Region	84 – 404	321	Catalytic By similarity
Sites
	Active site	295	1	Nucleophile Potential
Experimental info
	Sequence conflict	100	1	W → R in AAT93230. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P38130 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 199F184731C4F381	FASTA	404	47,482
	10 20 30 40 50 60 MSVHHKKKLM PKSALLIRKY QKGIRSSFIG LIIVLSFLFF MSGSRSPEVP IAQGTSVSRV 70 80 90 100 110 120 ASKDYLMPFT DKSQGVIHPV DDGKKEKGVM VTLARNSDLW NLVKSIRHVE DRFNNRYHYD 130 140 150 160 170 180 WVFLNDQPFS DEFKRVTSAL VSGKAKYGTI PKDHWSIPSW IDTEKFDEKR LAMGKLDIPY 190 200 210 220 230 240 GSSVPYRHMC RFQSGFIWRH PLLEEYEWFW RVDTDITLFC DIQYDIFKFL KVNNKKYGFI 250 260 270 280 290 300 LSVSEYERTI PTLWETTKKF IKKNPKFLHK NNLMKFISND DGDTYNMCHF WTNFEIGSLD 310 320 330 340 350 360 FFRSDAYREY FDYLDSSGGF FYERWGDAPV HSIAASLFLD KSEIHFFDGL GFHHPDFTSC 370 380 390 400 PIEQKIRLQN KCICEPSKDV TWTPDYFCTR KYFSAGNYKL PPGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 12.8 kb segment, on the right arm of chromosome II from Saccharomyces cerevisiae including part of the DUR1,2 gene, contains five putative new genes." Bussereau F., Mallet L., Gaillon L., Jacquet M. Yeast 9:797-806(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments." Inadome H., Noda Y., Adachi H., Yoda K. Mol. Cell. Biol. 25:7696-7710(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SVP26.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z21487 Genomic DNA. Translation: CAA79693.1. Z36074 Genomic DNA. Translation: CAA85169.1. AY693211 Genomic DNA. Translation: AAT93230.1. BK006936 Genomic DNA. Translation: DAA07323.1.
PIR	S34031.
RefSeq	NP_009764.3. NM_001178553.3.
3D structure databases
ProteinModelPortal	P38130.
SMR	P38130. Positions 85-395.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1723N.
IntAct	P38130. 16 interactions.
MINT	MINT-391567.
STRING	4932.YBR205W.
Protein family/group databases
CAZy	GT15. Glycosyltransferase Family 15.
Proteomic databases
PaxDb	P38130.
PeptideAtlas	P38130.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR205W; YBR205W; YBR205W.
GeneID	852504.
KEGG	sce:YBR205W. sce:YBR211C.
Organism-specific databases
CYGD	YBR205w.
SGD	S000000409. KTR3.
Phylogenomic databases
eggNOG	COG5020.
GeneTree	ENSGT00390000000831.
HOGENOM	HOG000160667.
KO	K10967. K11556.
OMA	LMKFISN.
OrthoDB	EOG43BQXM.
Gene expression databases
Genevestigator	P38130.
GermOnline	YBR205W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002685. Glyco_trans_15. [Graphical view]
Pfam	PF01793. Glyco_transf_15. 1 hit. [Graphical view]
PIRSF	PIRSF018153. Glyco_trans_15. 1 hit.
ProtoNet	Search...
Other
NextBio	971516.

Entry information

Entry name

KTR3_YEAST

Accession

Primary (citable) accession number: P38130
Secondary accession number(s): D6VQK3, E9P931

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program