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Protein

Transcription initiation factor TFIID subunit 5

Gene

TAF5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the DNA-binding general transcription factor complex TFIID and the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus.6 Publications

GO - Molecular functioni

  • chromatin binding Source: SGD
  • identical protein binding Source: IntAct
  • protein complex scaffold Source: SGD
  • ubiquitin binding Source: SGD

GO - Biological processi

  • chromatin organization Source: SGD
  • histone acetylation Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29139-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 5
Alternative name(s):
TAFII-90
TBP-associated factor 5
TBP-associated factor 90 kDa
Gene namesi
Name:TAF5
Synonyms:TAF90
Ordered Locus Names:YBR198C
ORF Names:YBR1410
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR198C.
SGDiS000000402. TAF5.

Subcellular locationi

GO - Cellular componenti

  • SAGA complex Source: SGD
  • SLIK (SAGA-like) complex Source: SGD
  • transcription factor TFIID complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512601 – 798Transcription initiation factor TFIID subunit 5Add BLAST798

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei299PhosphoserineCombined sources1
Modified residuei411PhosphoserineCombined sources1
Modified residuei415PhosphoserineCombined sources1
Modified residuei787PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38129.
PRIDEiP38129.

PTM databases

iPTMnetiP38129.

Interactioni

Subunit structurei

The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Component of the 1.8 MDa SAGA complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SGF73, SPT20/ADA5, SPT8, TAF12, TAF6, HFI1/ADA1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10, TAF9, SGF11 and SUS1. TAF5, TAF6, TAF9, TAF19, TAF12 and ADA1 seem to be present in 2 copies. SAGA is built of 5 distinct domains with specialized functions. Domain I (containing TRA1) probably represents the activator interaction surface. Domain II (containing TAF5 and TAF6, and probably TAF9 and TAF10), domain III (containing GCN5, TAF10, SPT7, TAF5 and ADA1, and probably ADA2, ADA3 and TAF12), and domain IV (containing HFI1/ADA1 and TAF6, and probably TAF9) are believed to play primarily an architectural role. Domain III also harbors the HAT activity. Domain V (containing SPT3 and SPT20, and probably SPT8) represents the TBP-interacting module, which may be associated transiently with SAGA. Component of the SALSA complex, which consists of at least TRA1, SPT7 (C-terminal truncated form), TAF5, ADA3, SPT20, TAF12, TAF6, HFI1, GCN5, ADA2 and SPT3. Component of the SLIK complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SPT20, RTG2, TAF12, TAF6, HFI1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10 and TAF9.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself9EBI-18868,EBI-18868
HFI1Q1206015EBI-18868,EBI-8287
TAF2P2325510EBI-18868,EBI-18862
TAF4P5010512EBI-18868,EBI-11231
TAF6P5304013EBI-18868,EBI-18876

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein complex scaffold Source: SGD
  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi32895. 306 interactors.
DIPiDIP-740N.
IntActiP38129. 198 interactors.
MINTiMINT-475651.

Structurei

Secondary structure

1798
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi152 – 164Combined sources13
Turni168 – 170Combined sources3
Helixi171 – 192Combined sources22
Helixi194 – 204Combined sources11
Helixi205 – 208Combined sources4
Helixi209 – 217Combined sources9
Turni218 – 221Combined sources4
Helixi225 – 230Combined sources6
Helixi232 – 238Combined sources7
Beta strandi242 – 246Combined sources5
Helixi248 – 260Combined sources13
Helixi262 – 264Combined sources3
Helixi266 – 276Combined sources11
Beta strandi277 – 281Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J49X-ray2.30A147-290[»]
ProteinModelPortaliP38129.
SMRiP38129.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38129.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 88LisHPROSITE-ProRule annotationAdd BLAST33
Repeati464 – 503WD 1Add BLAST40
Repeati523 – 562WD 2Add BLAST40
Repeati565 – 604WD 3Add BLAST40
Repeati607 – 646WD 4Add BLAST40
Repeati649 – 688WD 5Add BLAST40
Repeati692 – 731WD 6Add BLAST40

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili329 – 349Sequence analysisAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 48Gln-richAdd BLAST46

Sequence similaritiesi

Belongs to the WD repeat TAF5 family.Curated
Contains 1 LisH domain.PROSITE-ProRule annotation
Contains 6 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00860000133707.
HOGENOMiHOG000212424.
InParanoidiP38129.
KOiK03130.
OMAiEDARPKY.
OrthoDBiEOG092C10HM.

Family and domain databases

CDDicd08044. TAF5_NTD2. 1 hit.
Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR006594. LisH.
IPR007582. TFIID_NTD2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08513. LisH. 1 hit.
PF04494. TFIID_NTD2. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00667. LisH. 1 hit.
SM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS50896. LISH. 1 hit.
PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQKQSTNQN QNGTHQPQPV KNQRTNNAAG ANSGQQPQQQ SQGQSQQQGR
60 70 80 90 100
SNGPFSASDL NRIVLEYLNK KGYHRTEAML RAESGRTLTP QNKQSPANTK
110 120 130 140 150
TGKFPEQSSI PPNPGKTAKP ISNPTNLSSK RDAEGGIVSS GRLEGLNAPE
160 170 180 190 200
NYIRAYSMLK NWVDSSLEIY KPELSYIMYP IFIYLFLNLV AKNPVYARRF
210 220 230 240 250
FDRFSPDFKD FHGSEINRLF SVNSIDHIKE NEVASAFQSH KYRITMSKTT
260 270 280 290 300
LNLLLYFLNE NESIGGSLII SVINQHLDPN IVESVTAREK LADGIKVLSD
310 320 330 340 350
SENGNGKQNL EMNSVPVKLG PFPKDEEFVK EIETELKIKD DQEKQLNQQT
360 370 380 390 400
AGDNYSGANN RTLLQEYKAM NNEKFKDNTG DDDKDKIKDK IAKDEEKKES
410 420 430 440 450
ELKVDGEKKD SNLSSPARDI LPLPPKTALD LKLEIQKVKE SRDAIKLDNL
460 470 480 490 500
QLALPSVCMY TFQNTNKDMS CLDFSDDCRI AAAGFQDSYI KIWSLDGSSL
510 520 530 540 550
NNPNIALNNN DKDEDPTCKT LVGHSGTVYS TSFSPDNKYL LSGSEDKTVR
560 570 580 590 600
LWSMDTHTAL VSYKGHNHPV WDVSFSPLGH YFATASHDQT ARLWSCDHIY
610 620 630 640 650
PLRIFAGHLN DVDCVSFHPN GCYVFTGSSD KTCRMWDVST GDSVRLFLGH
660 670 680 690 700
TAPVISIAVC PDGRWLSTGS EDGIINVWDI GTGKRLKQMR GHGKNAIYSL
710 720 730 740 750
SYSKEGNVLI SGGADHTVRV WDLKKATTEP SAEPDEPFIG YLGDVTASIN
760 770 780 790
QDIKEYGRRR TVIPTSDLVA SFYTKKTPVF KVKFSRSNLA LAGGAFRP
Length:798
Mass (Da):88,968
Last modified:October 1, 1994 - v1
Checksum:iB42315B8C752D0B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21487 Genomic DNA. Translation: CAA79685.1.
Z36067 Genomic DNA. Translation: CAA85160.1.
AY692890 Genomic DNA. Translation: AAT92909.1.
BK006936 Genomic DNA. Translation: DAA07314.1.
PIRiS34023.
RefSeqiNP_009757.1. NM_001178546.1.

Genome annotation databases

EnsemblFungiiYBR198C; YBR198C; YBR198C.
GeneIDi852497.
KEGGisce:YBR198C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21487 Genomic DNA. Translation: CAA79685.1.
Z36067 Genomic DNA. Translation: CAA85160.1.
AY692890 Genomic DNA. Translation: AAT92909.1.
BK006936 Genomic DNA. Translation: DAA07314.1.
PIRiS34023.
RefSeqiNP_009757.1. NM_001178546.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J49X-ray2.30A147-290[»]
ProteinModelPortaliP38129.
SMRiP38129.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32895. 306 interactors.
DIPiDIP-740N.
IntActiP38129. 198 interactors.
MINTiMINT-475651.

PTM databases

iPTMnetiP38129.

Proteomic databases

MaxQBiP38129.
PRIDEiP38129.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR198C; YBR198C; YBR198C.
GeneIDi852497.
KEGGisce:YBR198C.

Organism-specific databases

EuPathDBiFungiDB:YBR198C.
SGDiS000000402. TAF5.

Phylogenomic databases

GeneTreeiENSGT00860000133707.
HOGENOMiHOG000212424.
InParanoidiP38129.
KOiK03130.
OMAiEDARPKY.
OrthoDBiEOG092C10HM.

Enzyme and pathway databases

BioCyciYEAST:G3O-29139-MONOMER.
ReactomeiR-SCE-674695. RNA Polymerase II Pre-transcription Events.
R-SCE-73776. RNA Polymerase II Promoter Escape.
R-SCE-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-SCE-75953. RNA Polymerase II Transcription Initiation.
R-SCE-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

EvolutionaryTraceiP38129.
PROiP38129.

Family and domain databases

CDDicd08044. TAF5_NTD2. 1 hit.
Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR006594. LisH.
IPR007582. TFIID_NTD2.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08513. LisH. 1 hit.
PF04494. TFIID_NTD2. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00667. LisH. 1 hit.
SM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS50896. LISH. 1 hit.
PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 6 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTAF5_YEAST
AccessioniPrimary (citable) accession number: P38129
Secondary accession number(s): D6VQJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 30, 2016
This is version 169 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 14834 (+/-203) molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.