ID   RIM2_YEAST              Reviewed;         377 AA.
AC   P38127; D6VQI6;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   27-MAR-2024, entry version 173.
DE   RecName: Full=Mitochondrial carrier protein RIM2;
GN   Name=RIM2; OrderedLocusNames=YBR192W; ORFNames=YBR1402;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8346681; DOI=10.1002/yea.320090611;
RA   Demolis N., Mallet L., Bussereau F., Jacquet M.;
RT   "RIM2, MSI1 and PGI1 are located within an 8 kb segment of Saccharomyces
RT   cerevisiae chromosome II, which also contains the putative ribosomal gene
RT   L21 and a new putative essential gene with a leucine zipper motif.";
RL   Yeast 9:645-659(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA   Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA   Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA   Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA   Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA   Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA   Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA   Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA   Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA   Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA   Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA   Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA   Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA   Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA   Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA   Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA   Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA   Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA   Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA   Mewes H.-W., Kleine K.;
RT   "Complete DNA sequence of yeast chromosome II.";
RL   EMBO J. 13:5795-5809(1994).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RX   PubMed=16823961; DOI=10.1021/pr050477f;
RA   Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.;
RT   "Toward the complete yeast mitochondrial proteome: multidimensional
RT   separation techniques for mitochondrial proteomics.";
RL   J. Proteome Res. 5:1543-1554(2006).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000269|PubMed:16823961}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:16823961}.
CC   -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC       {ECO:0000305}.
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DR   EMBL; Z21487; CAA79678.1; -; Genomic_DNA.
DR   EMBL; Z36061; CAA85154.1; -; Genomic_DNA.
DR   EMBL; AY557871; AAS56197.1; -; Genomic_DNA.
DR   EMBL; BK006936; DAA07306.1; -; Genomic_DNA.
DR   PIR; S36081; S36081.
DR   RefSeq; NP_009751.1; NM_001178540.1.
DR   AlphaFoldDB; P38127; -.
DR   SMR; P38127; -.
DR   BioGRID; 32889; 35.
DR   DIP; DIP-4933N; -.
DR   IntAct; P38127; 2.
DR   STRING; 4932.YBR192W; -.
DR   TCDB; 2.A.29.10.4; the mitochondrial carrier (mc) family.
DR   MaxQB; P38127; -.
DR   PaxDb; 4932-YBR192W; -.
DR   PeptideAtlas; P38127; -.
DR   EnsemblFungi; YBR192W_mRNA; YBR192W; YBR192W.
DR   GeneID; 852491; -.
DR   KEGG; sce:YBR192W; -.
DR   AGR; SGD:S000000396; -.
DR   SGD; S000000396; RIM2.
DR   VEuPathDB; FungiDB:YBR192W; -.
DR   eggNOG; KOG0757; Eukaryota.
DR   GeneTree; ENSGT00940000168859; -.
DR   HOGENOM; CLU_015166_6_0_1; -.
DR   InParanoid; P38127; -.
DR   OMA; WVMYEQM; -.
DR   OrthoDB; 4772at2759; -.
DR   BioCyc; YEAST:G3O-29134-MONOMER; -.
DR   BioGRID-ORCS; 852491; 1 hit in 10 CRISPR screens.
DR   PRO; PR:P38127; -.
DR   Proteomes; UP000002311; Chromosome II.
DR   RNAct; P38127; Protein.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR   GO; GO:0015218; F:pyrimidine nucleotide transmembrane transporter activity; IDA:SGD.
DR   GO; GO:0030001; P:metal ion transport; IDA:SGD.
DR   GO; GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
DR   GO; GO:1990519; P:pyrimidine nucleotide import into mitochondrion; IBA:GO_Central.
DR   GO; GO:0006864; P:pyrimidine nucleotide transport; IDA:SGD.
DR   GO; GO:0051881; P:regulation of mitochondrial membrane potential; IDA:UniProtKB.
DR   GO; GO:0055085; P:transmembrane transport; IDA:SGD.
DR   Gene3D; 1.50.40.10; Mitochondrial carrier domain; 2.
DR   InterPro; IPR002067; Mit_carrier.
DR   InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR   InterPro; IPR023395; Mt_carrier_dom_sf.
DR   InterPro; IPR049562; SLC25A33/36-like.
DR   PANTHER; PTHR45829; MITOCHONDRIAL CARRIER PROTEIN RIM2; 1.
DR   PANTHER; PTHR45829:SF4; MITOCHONDRIAL CARRIER PROTEIN RIM2; 1.
DR   Pfam; PF00153; Mito_carr; 3.
DR   PRINTS; PR00926; MITOCARRIER.
DR   SUPFAM; SSF103506; Mitochondrial carrier; 1.
DR   PROSITE; PS50920; SOLCAR; 3.
PE   1: Evidence at protein level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW   Repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..377
FT                   /note="Mitochondrial carrier protein RIM2"
FT                   /id="PRO_0000090690"
FT   TRANSMEM        53..73
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        131..151
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        179..199
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        238..258
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        286..306
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        347..368
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          50..163
FT                   /note="Solcar 1"
FT   REPEAT          173..262
FT                   /note="Solcar 2"
FT   REPEAT          286..375
FT                   /note="Solcar 3"
SQ   SEQUENCE   377 AA;  42102 MW;  A3C61383C3B3743B CRC64;
     MPKKSIEEWE EDAIESVPYL ASDEKGSNYK EATQIPLNLK QSEIENHPTV KPWVHFVAGG
     IGGMAGAVVT CPFDLVKTRL QSDIFLKAYK SQAVNISKGS TRPKSINYVI QAGTHFKETL
     GIIGNVYKQE GFRSLFKGLG PNLVGVIPAR SINFFTYGTT KDMYAKAFNN GQETPMIHLM
     AAATAGWATA TATNPIWLIK TRVQLDKAGK TSVRQYKNSW DCLKSVIRNE GFTGLYKGLS
     ASYLGSVEGI LQWLLYEQMK RLIKERSIEK FGYQAEGTKS TSEKVKEWCQ RSGSAGLAKF
     VASIATYPHE VVRTRLRQTP KENGKRKYTG LVQSFKVIIK EEGLFSMYSG LTPHLMRTVP
     NSIIMFGTWE IVIRLLS
//