Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pachytene checkpoint protein 2

Gene

PCH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the pachytene checkpoint, the meiotic checkpoint that prevents chromosome segregation when defects in recombination and synaptonemal complex formation occurred. Represses meiotic recombination in the rDNA, probably by excluding the meiosis-specific protein HOP1 from the nucleolar region.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi314 – 3218ATPSequence Analysis

GO - Molecular functioni

  1. ATPase activity Source: SGD
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. meiotic DNA double-strand break formation Source: SGD
  3. meiotic recombination checkpoint Source: SGD
  4. reciprocal meiotic recombination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29129-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pachytene checkpoint protein 2
Gene namesi
Name:PCH2
Ordered Locus Names:YBR186W
ORF Names:YBR1308
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR186w.
SGDiS000000390. PCH2.

Subcellular locationi

Nucleusnucleolus 1 Publication. Chromosome 1 Publication
Note: The majority of the protein localizes to the nucleolar region containing the ribosomal RNA genes (rDNA), a tandem array of 100-150 repeats on chromosome XII. Localization is SIR2-dependent.

GO - Cellular componenti

  1. chromosome Source: UniProtKB-SubCell
  2. nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 564564Pachytene checkpoint protein 2PRO_0000084789Add
BLAST

Expressioni

Inductioni

Induced at the onset of meiosis I and reaches a maximum level during pachytene (at protein level).1 Publication

Gene expression databases

GenevestigatoriP38126.

Interactioni

Protein-protein interaction databases

BioGridi32884. 30 interactions.
DIPiDIP-4661N.
IntActiP38126. 4 interactions.
MINTiMINT-559572.
STRINGi4932.YBR186W.

Structurei

3D structure databases

ProteinModelPortaliP38126.
SMRiP38126. Positions 211-506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family. PCH2 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00390000017432.
HOGENOMiHOG000115471.
InParanoidiP38126.
OMAiESGHERT.
OrthoDBiEOG7966S9.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.

Sequencei

Sequence statusi: Complete.

P38126-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSYIVDLQVR GSSLRVIKCM FREDEQISSL HSGSDSKQNS NKKLGEFLNL
60 70 80 90 100
LKAVVKRKLE SFPKDRLKTS IITGQELMRE GQGSIEIKDP PTEAQQHLIR
110 120 130 140 150
SLAKVLLHQF SSINGKVNTV NEGQDNLFLS LFVKKISIEQ QSTSHVSIKL
160 170 180 190 200
NFHEKINLGQ HIDSILDSEE TNESDTYHMG SVDEFIIYPF CCLEEQDELK
210 220 230 240 250
NGSILSTEFD KIDLELDEDD GFEGETLNNC INSVGNFDIP LSKQTLNLVN
260 270 280 290 300
ISYLPGTTFE GQWESLYFGN NIKERLYSYA TISLKIARFK QTGDSNQEDI
310 320 330 340 350
TTLITNNKLL LVHGPPGTGK TTLCKALCQK LSVRREFSDG SDTIDTNYKG
360 370 380 390 400
IIIELSCARI FSKWFGESSK NISIVFKDIE ELLKVNEGRG IFICLLIDEV
410 420 430 440 450
EAIASSRTNL SSRNESTDGI RVVNTLLTQL DRLKKYHNFL ALATSNLLDS
460 470 480 490 500
LDDAFVDRAD GVFYVGNPTA EGILHILKVC IEEMITSGII LFHARSTGVK
510 520 530 540 550
FFNKYQDILR KIAIKCSTVD ISGRTIRKLP LMCLSEYFRT FPVDDDEFVL
560
ALAMSARKLS AARK
Length:564
Mass (Da):63,460
Last modified:September 5, 2006 - v2
Checksum:i9B40C41368672877
GO

Sequence cautioni

The sequence AAB60280.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAA85147.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02073 Genomic DNA. Translation: AAB60280.1. Sequence problems.
Z36055 Genomic DNA. Translation: CAA85147.1. Sequence problems.
BK006936 Genomic DNA. Translation: DAA07301.1.
PIRiS46058.
RefSeqiNP_009745.2. NM_001178534.1.

Genome annotation databases

EnsemblFungiiYBR186W; YBR186W; YBR186W.
GeneIDi852484.
KEGGisce:YBR186W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U02073 Genomic DNA. Translation: AAB60280.1. Sequence problems.
Z36055 Genomic DNA. Translation: CAA85147.1. Sequence problems.
BK006936 Genomic DNA. Translation: DAA07301.1.
PIRiS46058.
RefSeqiNP_009745.2. NM_001178534.1.

3D structure databases

ProteinModelPortaliP38126.
SMRiP38126. Positions 211-506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32884. 30 interactions.
DIPiDIP-4661N.
IntActiP38126. 4 interactions.
MINTiMINT-559572.
STRINGi4932.YBR186W.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR186W; YBR186W; YBR186W.
GeneIDi852484.
KEGGisce:YBR186W.

Organism-specific databases

CYGDiYBR186w.
SGDiS000000390. PCH2.

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00390000017432.
HOGENOMiHOG000115471.
InParanoidiP38126.
OMAiESGHERT.
OrthoDBiEOG7966S9.

Enzyme and pathway databases

BioCyciYEAST:G3O-29129-MONOMER.

Miscellaneous databases

NextBioi971462.
PROiP38126.

Gene expression databases

GenevestigatoriP38126.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes encoding ribosomal proteins and six putative new genes, one of which encodes a putative transcriptional factor."
    Demolis N., Jacquet M., Mallet L.
    Yeast 10:1511-1525(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Pch2 links chromatin silencing to meiotic checkpoint control."
    San-Segundo P.A., Roeder G.S.
    Cell 97:313-324(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
  5. "Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
    Davis C.A., Grate L., Spingola M., Ares M. Jr.
    Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVISION OF GENE MODEL.
  6. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
    Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
    Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPCH2_YEAST
AccessioniPrimary (citable) accession number: P38126
Secondary accession number(s): D6VQI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: September 5, 2006
Last modified: January 7, 2015
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.