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P38126 (PCH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pachytene checkpoint protein 2
Gene names
Name:PCH2
Ordered Locus Names:YBR186W
ORF Names:YBR1308
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the pachytene checkpoint, the meiotic checkpoint that prevents chromosome segregation when defects in recombination and synaptonemal complex formation occurred. Represses meiotic recombination in the rDNA, probably by excluding the meiosis-specific protein HOP1 from the nucleolar region. Ref.4

Subcellular location

Nucleusnucleolus. Chromosome. Note: The majority of the protein localizes to the nucleolar region containing the ribosomal RNA genes (rDNA), a tandem array of 100-150 repeats on chromosome XII. Localization is SIR2-dependent. Ref.4

Induction

Induced at the onset of meiosis I and reaches a maximum level during pachytene (at protein level). Ref.4

Sequence similarities

Belongs to the AAA ATPase family. PCH2 subfamily.

Sequence caution

The sequence AAB60280.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAA85147.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 564564Pachytene checkpoint protein 2
PRO_0000084789

Regions

Nucleotide binding314 – 3218ATP Potential

Sequences

Sequence LengthMass (Da)Tools
P38126 [UniParc].

Last modified September 5, 2006. Version 2.
Checksum: 9B40C41368672877

FASTA56463,460
        10         20         30         40         50         60 
MSYIVDLQVR GSSLRVIKCM FREDEQISSL HSGSDSKQNS NKKLGEFLNL LKAVVKRKLE 

        70         80         90        100        110        120 
SFPKDRLKTS IITGQELMRE GQGSIEIKDP PTEAQQHLIR SLAKVLLHQF SSINGKVNTV 

       130        140        150        160        170        180 
NEGQDNLFLS LFVKKISIEQ QSTSHVSIKL NFHEKINLGQ HIDSILDSEE TNESDTYHMG 

       190        200        210        220        230        240 
SVDEFIIYPF CCLEEQDELK NGSILSTEFD KIDLELDEDD GFEGETLNNC INSVGNFDIP 

       250        260        270        280        290        300 
LSKQTLNLVN ISYLPGTTFE GQWESLYFGN NIKERLYSYA TISLKIARFK QTGDSNQEDI 

       310        320        330        340        350        360 
TTLITNNKLL LVHGPPGTGK TTLCKALCQK LSVRREFSDG SDTIDTNYKG IIIELSCARI 

       370        380        390        400        410        420 
FSKWFGESSK NISIVFKDIE ELLKVNEGRG IFICLLIDEV EAIASSRTNL SSRNESTDGI 

       430        440        450        460        470        480 
RVVNTLLTQL DRLKKYHNFL ALATSNLLDS LDDAFVDRAD GVFYVGNPTA EGILHILKVC 

       490        500        510        520        530        540 
IEEMITSGII LFHARSTGVK FFNKYQDILR KIAIKCSTVD ISGRTIRKLP LMCLSEYFRT 

       550        560 
FPVDDDEFVL ALAMSARKLS AARK 

« Hide

References

« Hide 'large scale' references
[1]"A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes encoding ribosomal proteins and six putative new genes, one of which encodes a putative transcriptional factor."
Demolis N., Jacquet M., Mallet L.
Yeast 10:1511-1525(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Pch2 links chromatin silencing to meiotic checkpoint control."
San-Segundo P.A., Roeder G.S.
Cell 97:313-324(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
[5]"Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
Davis C.A., Grate L., Spingola M., Ares M. Jr.
Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: REVISION OF GENE MODEL.
[6]"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U02073 Genomic DNA. Translation: AAB60280.1. Sequence problems.
Z36055 Genomic DNA. Translation: CAA85147.1. Sequence problems.
BK006936 Genomic DNA. Translation: DAA07301.1.
PIRS46058.
RefSeqNP_009745.2. NM_001178534.1.

3D structure databases

ProteinModelPortalP38126.
SMRP38126. Positions 211-557.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32884. 30 interactions.
DIPDIP-4661N.
IntActP38126. 4 interactions.
MINTMINT-559572.
STRING4932.YBR186W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR186W; YBR186W; YBR186W.
GeneID852484.
KEGGsce:YBR186W.

Organism-specific databases

CYGDYBR186w.
SGDS000000390. PCH2.

Phylogenomic databases

eggNOGCOG0464.
GeneTreeENSGT00390000017432.
HOGENOMHOG000115471.
OMAESGHERT.
OrthoDBEOG7966S9.

Enzyme and pathway databases

BioCycYEAST:G3O-29129-MONOMER.

Gene expression databases

GenevestigatorP38126.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00004. AAA. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 3 hits.
ProtoNetSearch...

Other

NextBio971462.
PROP38126.

Entry information

Entry namePCH2_YEAST
AccessionPrimary (citable) accession number: P38126
Secondary accession number(s): D6VQI1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: September 5, 2006
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families