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P38126

- PCH2_YEAST

UniProt

P38126 - PCH2_YEAST

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Protein

Pachytene checkpoint protein 2

Gene

PCH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Required for the pachytene checkpoint, the meiotic checkpoint that prevents chromosome segregation when defects in recombination and synaptonemal complex formation occurred. Represses meiotic recombination in the rDNA, probably by excluding the meiosis-specific protein HOP1 from the nucleolar region.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi314 – 3218ATPSequence Analysis

GO - Molecular functioni

  1. ATPase activity Source: SGD
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. meiotic DNA double-strand break formation Source: SGD
  3. meiotic recombination checkpoint Source: SGD
  4. reciprocal meiotic recombination Source: SGD
Complete GO annotation...

Keywords - Biological processi

Meiosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29129-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pachytene checkpoint protein 2
Gene namesi
Name:PCH2
Ordered Locus Names:YBR186W
ORF Names:YBR1308
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR186w.
SGDiS000000390. PCH2.

Subcellular locationi

Nucleusnucleolus 1 Publication. Chromosome 1 Publication
Note: The majority of the protein localizes to the nucleolar region containing the ribosomal RNA genes (rDNA), a tandem array of 100-150 repeats on chromosome XII. Localization is SIR2-dependent.

GO - Cellular componenti

  1. chromosome Source: UniProtKB-KW
  2. nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 564564Pachytene checkpoint protein 2PRO_0000084789Add
BLAST

Expressioni

Inductioni

Induced at the onset of meiosis I and reaches a maximum level during pachytene (at protein level).1 Publication

Gene expression databases

GenevestigatoriP38126.

Interactioni

Protein-protein interaction databases

BioGridi32884. 30 interactions.
DIPiDIP-4661N.
IntActiP38126. 4 interactions.
MINTiMINT-559572.
STRINGi4932.YBR186W.

Structurei

3D structure databases

ProteinModelPortaliP38126.
SMRiP38126. Positions 211-523.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family. PCH2 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0464.
GeneTreeiENSGT00390000017432.
HOGENOMiHOG000115471.
InParanoidiP38126.
OMAiESGHERT.
OrthoDBiEOG7966S9.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.

Sequencei

Sequence statusi: Complete.

P38126-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSYIVDLQVR GSSLRVIKCM FREDEQISSL HSGSDSKQNS NKKLGEFLNL
60 70 80 90 100
LKAVVKRKLE SFPKDRLKTS IITGQELMRE GQGSIEIKDP PTEAQQHLIR
110 120 130 140 150
SLAKVLLHQF SSINGKVNTV NEGQDNLFLS LFVKKISIEQ QSTSHVSIKL
160 170 180 190 200
NFHEKINLGQ HIDSILDSEE TNESDTYHMG SVDEFIIYPF CCLEEQDELK
210 220 230 240 250
NGSILSTEFD KIDLELDEDD GFEGETLNNC INSVGNFDIP LSKQTLNLVN
260 270 280 290 300
ISYLPGTTFE GQWESLYFGN NIKERLYSYA TISLKIARFK QTGDSNQEDI
310 320 330 340 350
TTLITNNKLL LVHGPPGTGK TTLCKALCQK LSVRREFSDG SDTIDTNYKG
360 370 380 390 400
IIIELSCARI FSKWFGESSK NISIVFKDIE ELLKVNEGRG IFICLLIDEV
410 420 430 440 450
EAIASSRTNL SSRNESTDGI RVVNTLLTQL DRLKKYHNFL ALATSNLLDS
460 470 480 490 500
LDDAFVDRAD GVFYVGNPTA EGILHILKVC IEEMITSGII LFHARSTGVK
510 520 530 540 550
FFNKYQDILR KIAIKCSTVD ISGRTIRKLP LMCLSEYFRT FPVDDDEFVL
560
ALAMSARKLS AARK
Length:564
Mass (Da):63,460
Last modified:September 5, 2006 - v2
Checksum:i9B40C41368672877
GO

Sequence cautioni

The sequence AAB60280.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAA85147.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02073 Genomic DNA. Translation: AAB60280.1. Sequence problems.
Z36055 Genomic DNA. Translation: CAA85147.1. Sequence problems.
BK006936 Genomic DNA. Translation: DAA07301.1.
PIRiS46058.
RefSeqiNP_009745.2. NM_001178534.1.

Genome annotation databases

EnsemblFungiiYBR186W; YBR186W; YBR186W.
GeneIDi852484.
KEGGisce:YBR186W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U02073 Genomic DNA. Translation: AAB60280.1 . Sequence problems.
Z36055 Genomic DNA. Translation: CAA85147.1 . Sequence problems.
BK006936 Genomic DNA. Translation: DAA07301.1 .
PIRi S46058.
RefSeqi NP_009745.2. NM_001178534.1.

3D structure databases

ProteinModelPortali P38126.
SMRi P38126. Positions 211-523.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32884. 30 interactions.
DIPi DIP-4661N.
IntActi P38126. 4 interactions.
MINTi MINT-559572.
STRINGi 4932.YBR186W.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR186W ; YBR186W ; YBR186W .
GeneIDi 852484.
KEGGi sce:YBR186W.

Organism-specific databases

CYGDi YBR186w.
SGDi S000000390. PCH2.

Phylogenomic databases

eggNOGi COG0464.
GeneTreei ENSGT00390000017432.
HOGENOMi HOG000115471.
InParanoidi P38126.
OMAi ESGHERT.
OrthoDBi EOG7966S9.

Enzyme and pathway databases

BioCyci YEAST:G3O-29129-MONOMER.

Miscellaneous databases

NextBioi 971462.
PROi P38126.

Gene expression databases

Genevestigatori P38126.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes encoding ribosomal proteins and six putative new genes, one of which encodes a putative transcriptional factor."
    Demolis N., Jacquet M., Mallet L.
    Yeast 10:1511-1525(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Pch2 links chromatin silencing to meiotic checkpoint control."
    San-Segundo P.A., Roeder G.S.
    Cell 97:313-324(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
  5. "Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
    Davis C.A., Grate L., Spingola M., Ares M. Jr.
    Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVISION OF GENE MODEL.
  6. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
    Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
    Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPCH2_YEAST
AccessioniPrimary (citable) accession number: P38126
Secondary accession number(s): D6VQI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: September 5, 2006
Last modified: October 29, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3