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P38126

- PCH2_YEAST

UniProt

P38126 - PCH2_YEAST

Protein

Pachytene checkpoint protein 2

Gene

PCH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Required for the pachytene checkpoint, the meiotic checkpoint that prevents chromosome segregation when defects in recombination and synaptonemal complex formation occurred. Represses meiotic recombination in the rDNA, probably by excluding the meiosis-specific protein HOP1 from the nucleolar region.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi314 – 3218ATPSequence Analysis

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. meiotic DNA double-strand break formation Source: SGD
    3. meiotic recombination checkpoint Source: SGD
    4. reciprocal meiotic recombination Source: SGD

    Keywords - Biological processi

    Meiosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29129-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pachytene checkpoint protein 2
    Gene namesi
    Name:PCH2
    Ordered Locus Names:YBR186W
    ORF Names:YBR1308
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR186w.
    SGDiS000000390. PCH2.

    Subcellular locationi

    Nucleusnucleolus 1 Publication. Chromosome 1 Publication
    Note: The majority of the protein localizes to the nucleolar region containing the ribosomal RNA genes (rDNA), a tandem array of 100-150 repeats on chromosome XII. Localization is SIR2-dependent.

    GO - Cellular componenti

    1. chromosome Source: UniProtKB-SubCell
    2. nucleolus Source: SGD

    Keywords - Cellular componenti

    Chromosome, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 564564Pachytene checkpoint protein 2PRO_0000084789Add
    BLAST

    Expressioni

    Inductioni

    Induced at the onset of meiosis I and reaches a maximum level during pachytene (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriP38126.

    Interactioni

    Protein-protein interaction databases

    BioGridi32884. 30 interactions.
    DIPiDIP-4661N.
    IntActiP38126. 4 interactions.
    MINTiMINT-559572.
    STRINGi4932.YBR186W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38126.
    SMRiP38126. Positions 211-556.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family. PCH2 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0464.
    GeneTreeiENSGT00390000017432.
    HOGENOMiHOG000115471.
    OMAiESGHERT.
    OrthoDBiEOG7966S9.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    P38126-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYIVDLQVR GSSLRVIKCM FREDEQISSL HSGSDSKQNS NKKLGEFLNL    50
    LKAVVKRKLE SFPKDRLKTS IITGQELMRE GQGSIEIKDP PTEAQQHLIR 100
    SLAKVLLHQF SSINGKVNTV NEGQDNLFLS LFVKKISIEQ QSTSHVSIKL 150
    NFHEKINLGQ HIDSILDSEE TNESDTYHMG SVDEFIIYPF CCLEEQDELK 200
    NGSILSTEFD KIDLELDEDD GFEGETLNNC INSVGNFDIP LSKQTLNLVN 250
    ISYLPGTTFE GQWESLYFGN NIKERLYSYA TISLKIARFK QTGDSNQEDI 300
    TTLITNNKLL LVHGPPGTGK TTLCKALCQK LSVRREFSDG SDTIDTNYKG 350
    IIIELSCARI FSKWFGESSK NISIVFKDIE ELLKVNEGRG IFICLLIDEV 400
    EAIASSRTNL SSRNESTDGI RVVNTLLTQL DRLKKYHNFL ALATSNLLDS 450
    LDDAFVDRAD GVFYVGNPTA EGILHILKVC IEEMITSGII LFHARSTGVK 500
    FFNKYQDILR KIAIKCSTVD ISGRTIRKLP LMCLSEYFRT FPVDDDEFVL 550
    ALAMSARKLS AARK 564
    Length:564
    Mass (Da):63,460
    Last modified:September 5, 2006 - v2
    Checksum:i9B40C41368672877
    GO

    Sequence cautioni

    The sequence AAB60280.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence CAA85147.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02073 Genomic DNA. Translation: AAB60280.1. Sequence problems.
    Z36055 Genomic DNA. Translation: CAA85147.1. Sequence problems.
    BK006936 Genomic DNA. Translation: DAA07301.1.
    PIRiS46058.
    RefSeqiNP_009745.2. NM_001178534.1.

    Genome annotation databases

    EnsemblFungiiYBR186W; YBR186W; YBR186W.
    GeneIDi852484.
    KEGGisce:YBR186W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02073 Genomic DNA. Translation: AAB60280.1 . Sequence problems.
    Z36055 Genomic DNA. Translation: CAA85147.1 . Sequence problems.
    BK006936 Genomic DNA. Translation: DAA07301.1 .
    PIRi S46058.
    RefSeqi NP_009745.2. NM_001178534.1.

    3D structure databases

    ProteinModelPortali P38126.
    SMRi P38126. Positions 211-556.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32884. 30 interactions.
    DIPi DIP-4661N.
    IntActi P38126. 4 interactions.
    MINTi MINT-559572.
    STRINGi 4932.YBR186W.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR186W ; YBR186W ; YBR186W .
    GeneIDi 852484.
    KEGGi sce:YBR186W.

    Organism-specific databases

    CYGDi YBR186w.
    SGDi S000000390. PCH2.

    Phylogenomic databases

    eggNOGi COG0464.
    GeneTreei ENSGT00390000017432.
    HOGENOMi HOG000115471.
    OMAi ESGHERT.
    OrthoDBi EOG7966S9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29129-MONOMER.

    Miscellaneous databases

    NextBioi 971462.
    PROi P38126.

    Gene expression databases

    Genevestigatori P38126.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes encoding ribosomal proteins and six putative new genes, one of which encodes a putative transcriptional factor."
      Demolis N., Jacquet M., Mallet L.
      Yeast 10:1511-1525(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Pch2 links chromatin silencing to meiotic checkpoint control."
      San-Segundo P.A., Roeder G.S.
      Cell 97:313-324(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
    5. "Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast."
      Davis C.A., Grate L., Spingola M., Ares M. Jr.
      Nucleic Acids Res. 28:1700-1706(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVISION OF GENE MODEL.
    6. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
      Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
      Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiPCH2_YEAST
    AccessioniPrimary (citable) accession number: P38126
    Secondary accession number(s): D6VQI1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3