Reviewed, UniProtKB/Swiss-Prot P38122 (PANB_YEAST)

Names and origin

Protein names

Recommended name:
    3-methyl-2-oxobutanoate hydroxymethyltransferase
    EC=2.1.2.11
Alternative name(s):
    Ketopantoate hydroxymethyltransferase
    Extracellular matrix protein 31

Gene names

Name:	ECM31
Ordered Locus Names:	YBR176W
ORF Names:	YBR1238

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.
Miscellaneous	Present with 1420 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the panB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Ref.2 Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Ref.2 Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
KAP95	Q06142	1	EBI-12901,EBI-9145
MUK1	Q02866	1	EBI-12901,EBI-32646
SRP1	Q02821	1	EBI-12901,EBI-1797
TEM1	P38987	1	EBI-12901,EBI-19113

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	3-methyl-2-oxobutanoate hydroxymethyltransferase		PRO_0000184927

Sequences

	Sequence		Length	Mass (Da)
	P38122-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 42A15F8A7A8CF291	FASTA	312	34,465
	10 20 30 40 50 60 MNIMKRQLCT SSKRFFSTAK NVVKYNTIQD IRNKYFTGTP LSMCTAYDFI TATWVNKANC 70 80 90 100 110 120 DLLLVGDSLA MTSLGYDSTI TLSLNEFKYH VASVCRAEGS SMVVVDMPFG TFESGISDGL 130 140 150 160 170 180 KNAIDIMKLD SKVTSVKVEV GSYTKDKYAM KFIEELCSRG IPVMAHIGLT PQKVHSLGGY 190 200 210 220 230 240 KVQGSKSLLQ MQELYETAMQ LQKIGCWSIL IECVPHKMAQ FITSKLSVPT IGIGAGNGTS 250 260 270 280 290 300 GQVLVISDLL GMQGDSVPKF VKQAVNMTDI ATQGLKEYIA SVEDRTFPER GTHTFKVKED 310 LWNEFLSSIN EK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Saccharomyces cerevisiae is capable of de novo pantothenic acid biosynthesis involving a novel pathway of beta-alanine production from spermine." White W.H., Gunyuzlu P.L., Toyn J.H. J. Biol. Chem. 276:10794-10800(2001) [PubMed: 11154694] [Abstract] Cited for: CHARACTERIZATION.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z36045 Genomic DNA. Translation: CAA85137.1.
PIR	S46047.
RefSeq	NP_009735.1.
3D structure databases
HSSP	HSSP built from PDB template 1OY0 based on UniProtKB Q10505.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1303N.
IntAct	P38122. 9 interactions.
STRING	P38122.
Genome annotation databases
Ensembl	YBR176W; YBR176W; YBR176W; Saccharomyces cerevisiae. [Genome view]
GeneID	852474.
GenomeReviews	Gene locus YBR176W in contig Y13134_GR.
KEGG	sce:YBR176W.
NMPDR	fig|4932.3.peg.441.
Organism-specific databases
CYGD	YBR176w.
SGD	S000000380. ECM31.
Phylogenomic databases
HOGENOM	P38122.
OMA	YATPEQT.
Enzyme and pathway databases
BRENDA	2.1.2.11. 250.
Gene expression databases
ArrayExpress	P38122.
Genevestigator	P38122.
GermOnline	YBR176W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
TIGRFAMs	TIGR00222. panB. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	971435.

Entry information

Entry name

PANB_YEAST

Accession

Primary (citable) accession number: P38122

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)