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Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase

Gene

ECM31

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 1420 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (ECM31)
  2. 2-dehydropantoate 2-reductase (PAN5)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • 3-methyl-2-oxobutanoate hydroxymethyltransferase activity Source: SGD

GO - Biological processi

  • pantothenate biosynthetic process Source: SGD

Keywordsi

Molecular functionTransferase
Biological processPantothenate biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-90
YEAST:MONOMER3O-90
UniPathwayiUPA00028; UER00003

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase (EC:2.1.2.11)
Alternative name(s):
Extracellular matrix protein 31
Ketopantoate hydroxymethyltransferase
Gene namesi
Name:ECM31
Ordered Locus Names:YBR176W
ORF Names:YBR1238
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR176W
SGDiS000000380 ECM31

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001849271 – 3123-methyl-2-oxobutanoate hydroxymethyltransferaseAdd BLAST312

Proteomic databases

MaxQBiP38122
PaxDbiP38122
PRIDEiP38122

Interactioni

Protein-protein interaction databases

BioGridi32875, 39 interactors
DIPiDIP-1303N
IntActiP38122, 1 interactor
MINTiP38122
STRINGi4932.YBR176W

Structurei

3D structure databases

ProteinModelPortaliP38122
SMRiP38122
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PanB family.Curated

Phylogenomic databases

HOGENOMiHOG000078427
InParanoidiP38122
KOiK00606
OMAiGHIGLMP
OrthoDBiEOG092C3I9E

Family and domain databases

CDDicd06557 KPHMT-like, 1 hit
HAMAPiMF_00156 PanB, 1 hit
InterProiView protein in InterPro
IPR003700 Pantoate_hydroxy_MeTrfase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR20881 PTHR20881, 1 hit
PfamiView protein in Pfam
PF02548 Pantoate_transf, 1 hit
PIRSFiPIRSF000388 Pantoate_hydroxy_MeTrfase, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR00222 panB, 1 hit

Sequencei

Sequence statusi: Complete.

P38122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIMKRQLCT SSKRFFSTAK NVVKYNTIQD IRNKYFTGTP LSMCTAYDFI
60 70 80 90 100
TATWVNKANC DLLLVGDSLA MTSLGYDSTI TLSLNEFKYH VASVCRAEGS
110 120 130 140 150
SMVVVDMPFG TFESGISDGL KNAIDIMKLD SKVTSVKVEV GSYTKDKYAM
160 170 180 190 200
KFIEELCSRG IPVMAHIGLT PQKVHSLGGY KVQGSKSLLQ MQELYETAMQ
210 220 230 240 250
LQKIGCWSIL IECVPHKMAQ FITSKLSVPT IGIGAGNGTS GQVLVISDLL
260 270 280 290 300
GMQGDSVPKF VKQAVNMTDI ATQGLKEYIA SVEDRTFPER GTHTFKVKED
310
LWNEFLSSIN EK
Length:312
Mass (Da):34,465
Last modified:October 1, 1994 - v1
Checksum:i42A15F8A7A8CF291
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36045 Genomic DNA Translation: CAA85137.1
BK006936 Genomic DNA Translation: DAA07291.1
PIRiS46047
RefSeqiNP_009735.3, NM_001178524.3

Genome annotation databases

EnsemblFungiiYBR176W; YBR176W; YBR176W
GeneIDi852474
KEGGisce:YBR176W

Similar proteinsi

Entry informationi

Entry nameiPANB_YEAST
AccessioniPrimary (citable) accession number: P38122
Secondary accession number(s): D6VQH1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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