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Protein

DNA polymerase alpha subunit B

Gene

POL12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-catalytic component of DNA polymerase alpha, which in a complex with DNA primase (DNA polymerase alpha:primase) constitutes a replicative polymerase. POL12 may play an essential role at the early stage of chromosomal DNA replication by coupling DNA polymerase alpha to the cellular replication machinery (By similarity). Interacts with MCM10.By similarity

Miscellaneous

Present with 11200 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • DNA replication initiation Source: SGD
  • lagging strand elongation Source: SGD
  • telomere capping Source: SGD

Keywordsi

Biological processDNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-28937-MONOMER
ReactomeiR-SCE-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-SCE-174411 Polymerase switching on the C-strand of the telomere
R-SCE-174430 Telomere C-strand synthesis initiation
R-SCE-68952 DNA replication initiation
R-SCE-68962 Activation of the pre-replicative complex
R-SCE-69091 Polymerase switching
R-SCE-69166 Removal of the Flap Intermediate
R-SCE-69183 Processive synthesis on the lagging strand

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase alpha subunit B
Alternative name(s):
DNA polymerase I subunit B
DNA polymerase alpha:primase complex p86 subunit
Short name:
Pol alpha-primase complex p86 subunit
DNA polymerase-primase complex p74 subunit
Gene namesi
Name:POL12
Ordered Locus Names:YBL035C
ORF Names:YBL0414
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL035C
SGDiS000000131 POL12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940411 – 705DNA polymerase alpha subunit BAdd BLAST705

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei126PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated in a cell cycle-dependent manner.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38121
PaxDbiP38121
PRIDEiP38121

PTM databases

iPTMnetiP38121

Interactioni

Subunit structurei

DNA polymerase alpha:primase is a four subunit enzyme complex, which is assembled throughout the cell cycle, and consists of the two DNA polymerase subunits A and B, and the DNA primase large and small subunits. Subunit B binds to subunit A (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi32662, 409 interactors
DIPiDIP-2536N
IntActiP38121, 34 interactors
MINTiP38121
STRINGi4932.YBL035C

Structurei

Secondary structure

1705
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi254 – 276Combined sources23
Helixi280 – 282Combined sources3
Beta strandi294 – 306Combined sources13
Beta strandi315 – 319Combined sources5
Helixi322 – 325Combined sources4
Beta strandi329 – 333Combined sources5
Beta strandi340 – 342Combined sources3
Beta strandi347 – 353Combined sources7
Beta strandi355 – 358Combined sources4
Beta strandi360 – 365Combined sources6
Beta strandi375 – 377Combined sources3
Helixi378 – 388Combined sources11
Beta strandi393 – 400Combined sources8
Helixi411 – 422Combined sources12
Beta strandi427 – 433Combined sources7
Beta strandi435 – 437Combined sources3
Helixi441 – 445Combined sources5
Helixi461 – 468Combined sources8
Helixi470 – 473Combined sources4
Beta strandi480 – 485Combined sources6
Beta strandi497 – 500Combined sources4
Turni505 – 509Combined sources5
Turni512 – 514Combined sources3
Beta strandi515 – 517Combined sources3
Beta strandi520 – 526Combined sources7
Beta strandi529 – 533Combined sources5
Helixi538 – 541Combined sources4
Beta strandi545 – 547Combined sources3
Helixi549 – 553Combined sources5
Helixi556 – 567Combined sources12
Helixi614 – 620Combined sources7
Helixi623 – 625Combined sources3
Beta strandi629 – 632Combined sources4
Beta strandi640 – 644Combined sources5
Beta strandi647 – 651Combined sources5
Beta strandi664 – 670Combined sources7
Beta strandi675 – 678Combined sources4
Beta strandi680 – 683Combined sources4
Beta strandi685 – 687Combined sources3
Beta strandi689 – 691Combined sources3
Helixi694 – 697Combined sources4
Beta strandi698 – 704Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FLOX-ray2.50A/C/E/G246-705[»]
ProteinModelPortaliP38121
SMRiP38121
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38121

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi80 – 83Poly-Ser4

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000016784
HOGENOMiHOG000248502
InParanoidiP38121
KOiK02321
OMAiPDVMAEC
OrthoDBiEOG092C1WBG

Family and domain databases

InterProiView protein in InterPro
IPR007185 DNA_pol_alpha/epsilon_bsu
IPR016722 DNA_pol_alpha_bsu
IPR013627 Pol_alpha_B_N
PANTHERiPTHR23061 PTHR23061, 1 hit
PfamiView protein in Pfam
PF04042 DNA_pol_E_B, 1 hit
PF08418 Pol_alpha_B_N, 1 hit
PIRSFiPIRSF018300 DNA_pol_alph_2, 1 hit

Sequencei

Sequence statusi: Complete.

P38121-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSIDVITH FGPDADKPEI ITALENLTKL HALSVEDLYI KWEQFSNQRR
60 70 80 90 100
QTHTDLTSKN IDEFKQFLQL QMEKRANQIS SSSKVNTSTK KPVIKKSLNS
110 120 130 140 150
SPLFGLSIPK TPTLKKRKLH GPFSLSDSKQ TYNVGSEAET NEKGNSSLKL
160 170 180 190 200
EFTPGMAEDA VGDSAPLSHA KSSDAKTPGS STFQTPTTNT PTTSRQNVPA
210 220 230 240 250
GEILDSLNPE NIEISSGNPN VGLLSTEEPS YNQVKVEPFY DAKKYKFRTM
260 270 280 290 300
RQNLQEASDV LDDQIESFTK IIQNHYKLSP NDFADPTIQS QSEIYAVGRI
310 320 330 340 350
VPDSPTYDKF LNPESLSLET SRMGGVGRRV RLDLSQVNEL SFFLGQIVAF
360 370 380 390 400
KGKNANGDYF TVNSILPLPY PNSPVSTSQE LQEFQANLEG SSLKVIVTCG
410 420 430 440 450
PYFANDNFSL ELLQEFIDSI NNEVKPHVLI MFGPFIDITH PLIASGKLPN
460 470 480 490 500
FPQFKTQPKT LDELFLKLFT PILKTISPHI QTVLIPSTKD AISNHAAYPQ
510 520 530 540 550
ASLIRKALQL PKRNFKCMAN PSSFQINEIY FGCSNVDTFK DLKEVIKGGT
560 570 580 590 600
TSSRYRLDRV SEHILQQRRY YPIFPGSIRT RIKPKDVSTK KETNDMESKE
610 620 630 640 650
EKVYEHISGA DLDVSYLGLT EFVGGFSPDI MIIPSELQHF ARVVQNVVVI
660 670 680 690 700
NPGRFIRATG NRGSYAQITV QCPDLEDGKL TLVEGEEPVY LHNVWKRARV

DLIAS
Length:705
Mass (Da):78,774
Last modified:October 1, 1994 - v1
Checksum:iF9F06D12F6979637
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35796 Genomic DNA Translation: CAA84855.1
X74738 Genomic DNA Translation: CAA52761.1
BK006936 Genomic DNA Translation: DAA07084.1
PIRiS45769
RefSeqiNP_009518.1, NM_001178275.1

Genome annotation databases

EnsemblFungiiYBL035C; YBL035C; YBL035C
GeneIDi852245
KEGGisce:YBL035C

Similar proteinsi

Entry informationi

Entry nameiDPOA2_YEAST
AccessioniPrimary (citable) accession number: P38121
Secondary accession number(s): D6VPW4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: March 28, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health