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Protein

ADP-ribosylation factor-like protein 1

Gene

ARL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recruits golgins such as IMH1 to the Golgi. Can bind and hydrolyze GTP. May be involved in trafficking events within the endosomal system.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi25 – 328GTPBy similarity
Nucleotide bindingi68 – 725GTPBy similarity
Nucleotide bindingi127 – 1304GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • endocytosis Source: SGD
  • Golgi to plasma membrane protein transport Source: SGD
  • protein targeting to vacuole Source: SGD
  • small GTPase mediated signal transduction Source: InterPro
  • vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29114-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 1
Alternative name(s):
Arf-like GTPase 1
Gene namesi
Name:ARL1
Synonyms:ARF3
Ordered Locus Names:YBR164C
ORF Names:YBR1216
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR164C.
SGDiS000000368. ARL1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • Golgi apparatus Source: SGD
  • trans-Golgi network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 183182ADP-ribosylation factor-like protein 1PRO_0000207421Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiP38116.

Interactioni

Subunit structurei

Homodimer. Binds in a GTP-dependent manner to the GRIP domain of IMH1. Interacts with MON2.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
IMH1Q067043EBI-2869,EBI-33343
VPS53P470613EBI-2869,EBI-25828
VPS54Q120713EBI-2869,EBI-36751

Protein-protein interaction databases

BioGridi32864. 267 interactions.
IntActiP38116. 8 interactions.
MINTiMINT-656563.

Structurei

Secondary structure

1
183
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 97Combined sources
Helixi10 – 123Combined sources
Beta strandi19 – 268Combined sources
Helixi31 – 377Combined sources
Beta strandi41 – 466Combined sources
Beta strandi54 – 596Combined sources
Beta strandi62 – 698Combined sources
Helixi79 – 813Combined sources
Turni82 – 854Combined sources
Beta strandi86 – 949Combined sources
Turni98 – 1003Combined sources
Helixi101 – 11111Combined sources
Beta strandi121 – 1277Combined sources
Helixi137 – 1437Combined sources
Turni144 – 1485Combined sources
Beta strandi154 – 1607Combined sources
Helixi161 – 1633Combined sources
Helixi167 – 18216Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MOZX-ray3.17A/B1-183[»]
ProteinModelPortaliP38116.
SMRiP38116. Positions 2-183.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38116.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

GeneTreeiENSGT00830000128269.
HOGENOMiHOG000163691.
InParanoidiP38116.
KOiK07942.
OMAiDMEGCMS.
OrthoDBiEOG7FNCKB.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38116-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNIFSSMFD KLWGSNKELR ILILGLDGAG KTTILYRLQI GEVVTTKPTI
60 70 80 90 100
GFNVETLSYK NLKLNVWDLG GQTSIRPYWR CYYADTAAVI FVVDSTDKDR
110 120 130 140 150
MSTASKELHL MLQEEELQDA ALLVFANKQD QPGALSASEV SKELNLVELK
160 170 180
DRSWSIVASS AIKGEGITEG LDWLIDVIKE EQL
Length:183
Mass (Da):20,434
Last modified:January 23, 2007 - v4
Checksum:i84D0E1F79EC21E38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89332 mRNA. Translation: AAC49875.1.
Z36033 Genomic DNA. Translation: CAA85125.1.
BK006936 Genomic DNA. Translation: DAA07280.1.
PIRiS46035.
RefSeqiNP_009723.3. NM_001178512.3.

Genome annotation databases

EnsemblFungiiYBR164C; YBR164C; YBR164C.
GeneIDi852462.
KEGGisce:YBR164C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89332 mRNA. Translation: AAC49875.1.
Z36033 Genomic DNA. Translation: CAA85125.1.
BK006936 Genomic DNA. Translation: DAA07280.1.
PIRiS46035.
RefSeqiNP_009723.3. NM_001178512.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MOZX-ray3.17A/B1-183[»]
ProteinModelPortaliP38116.
SMRiP38116. Positions 2-183.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32864. 267 interactions.
IntActiP38116. 8 interactions.
MINTiMINT-656563.

Proteomic databases

MaxQBiP38116.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR164C; YBR164C; YBR164C.
GeneIDi852462.
KEGGisce:YBR164C.

Organism-specific databases

EuPathDBiFungiDB:YBR164C.
SGDiS000000368. ARL1.

Phylogenomic databases

GeneTreeiENSGT00830000128269.
HOGENOMiHOG000163691.
InParanoidiP38116.
KOiK07942.
OMAiDMEGCMS.
OrthoDBiEOG7FNCKB.

Enzyme and pathway databases

BioCyciYEAST:G3O-29114-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP38116.
PROiP38116.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of an ADP-ribosylation factor-like 1 protein in Saccharomyces cerevisiae."
    Lee F.-J.S., Huang C.F., Yu W.L., Buu L.M., Lin C.Y., Huang M.C., Moss J., Vaughan M.
    J. Biol. Chem. 272:30998-31005(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, MYRISTOYLATION AT GLY-2.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Yeast Ysl2p, homologous to Sec7 domain guanine nucleotide exchange factors, functions in endocytosis and maintenance of vacuole integrity and interacts with the Arf-Like small GTPase Arl1p."
    Jochum A., Jackson D., Schwarz H., Pipkorn R., Singer-Krueger B.
    Mol. Cell. Biol. 22:4914-4928(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MON2.
  5. "Golgi recruitment of GRIP domain proteins by Arf-like GTPase 1 is regulated by Arf-like GTPase 3."
    Setty S.R.G., Shin M.E., Yoshino A., Marks M.S., Burd C.G.
    Curr. Biol. 13:401-404(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH IMH1.
  6. "The ARF-like GTPases Arl1p and Arl3p act in a pathway that interacts with vesicle-tethering factors at the Golgi apparatus."
    Panic B., Whyte J.R.C., Munro S.
    Curr. Biol. 13:405-410(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH IMH1.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Structures of yeast ARF2 and ARL1: distinct roles for the N-terminus in the structure and function of ARF family GTPases."
    Amor J.C., Horton J.R., Zhu X., Wang Y., Sullards C., Ringe D., Cheng X., Kahn R.A.
    J. Biol. Chem. 276:42477-42484(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.17 ANGSTROMS).

Entry informationi

Entry nameiARL1_YEAST
AccessioniPrimary (citable) accession number: P38116
Secondary accession number(s): D6VQG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 149 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5190 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.