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P38115

- ARA1_YEAST

UniProt

P38115 - ARA1_YEAST

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Protein
D-arabinose dehydrogenase [NAD(P)+] heavy chain
Gene
ARA1, YBR149W, YBR1127
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of D-arabinose, L-xylose, L-fucose and L-galactose in the presence of NADP+.

Catalytic activityi

D-arabinose + NAD(P)+ = D-arabinono-1,4-lactone + NAD(P)H.

pH dependencei

Optimum pH is 10.0.

Temperature dependencei

Optimum temperature is about 30 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711Proton donor By similarity
Sitei100 – 1001Lowers pKa of active site Tyr By similarity
Binding sitei131 – 1311Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi241 – 29555NADP By similarity
Add
BLAST

GO - Molecular functioni

  1. D-arabinose 1-dehydrogenase [NAD(P)+] activity Source: SGD
  2. alditol:NADP+ 1-oxidoreductase activity Source: SGD

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-32366.
MetaCyc:MONOMER3O-32367.
YEAST:MONOMER3O-32366.
YEAST:MONOMER3O-32367.
ReactomeiREACT_189323. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
REACT_94166. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.

Names & Taxonomyi

Protein namesi
Recommended name:
D-arabinose dehydrogenase [NAD(P)+] heavy chain (EC:1.1.1.117)
Alternative name(s):
AKR3C
Gene namesi
Name:ARA1
Ordered Locus Names:YBR149W
ORF Names:YBR1127
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR149w.
SGDiS000000353. ARA1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344D-arabinose dehydrogenase [NAD(P)+] heavy chain
PRO_0000124610Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei151 – 1511Phosphothreonine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38115.
PaxDbiP38115.
PeptideAtlasiP38115.

Expressioni

Gene expression databases

GenevestigatoriP38115.

Interactioni

Subunit structurei

Heterodimer of a heavy chain and a light chain.

Protein-protein interaction databases

BioGridi32848. 21 interactions.
DIPiDIP-4926N.
MINTiMINT-521486.
STRINGi4932.YBR149W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi20 – 223
Beta strandi23 – 264
Beta strandi32 – 365
Helixi44 – 496
Helixi50 – 5910
Beta strandi64 – 663
Helixi69 – 713
Helixi74 – 8613
Helixi92 – 943
Beta strandi96 – 1016
Helixi103 – 1053
Helixi109 – 12012
Beta strandi126 – 1305
Helixi167 – 17913
Beta strandi185 – 1873
Beta strandi189 – 1935
Helixi196 – 20510
Beta strandi211 – 2166
Helixi224 – 23310
Beta strandi236 – 2416
Helixi249 – 2524
Helixi254 – 2629
Helixi267 – 27711
Helixi289 – 2946
Helixi303 – 31513
Helixi324 – 3263
Helixi339 – 3413

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IJCX-ray2.10A/B1-344[»]
4IJRX-ray2.00A/C1-344[»]
ProteinModelPortaliP38115.
SMRiP38115. Positions 15-342.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0656.
GeneTreeiENSGT00750000117803.
HOGENOMiHOG000250272.
KOiK00063.
OrthoDBiEOG7WQ83S.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38115-1 [UniParc]FASTAAdd to Basket

« Hide

MSSSVASTEN IVENMLHPKT TEIYFSLNNG VRIPALGLGT ANPHEKLAET    50
KQAVKAAIKA GYRHIDTAWA YETEPFVGEA IKELLEDGSI KREDLFITTK 100
VWPVLWDEVD RSLNESLKAL GLEYVDLLLQ HWPLCFEKIK DPKGISGLVK 150
TPVDDSGKTM YAADGDYLET YKQLEKIYLD PNDHRVRAIG VSNFSIEYLE 200
RLIKECRVKP TVNQVETHPH LPQMELRKFC FMHDILLTAY SPLGSHGAPN 250
LKIPLVKKLA EKYNVTGNDL LISYHIRQGT IVIPRSLNPV RISSSIEFAS 300
LTKDELQELN DFGEKYPVRF IDEPFAAILP EFTGNGPNLD NLKY 344
Length:344
Mass (Da):38,884
Last modified:October 1, 1994 - v1
Checksum:iF9B6D9333B18FEEC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 627AAIKAGY → LQSKLDN in AAA35037. 1 Publication
Sequence conflicti71 – 733YET → SR in AAA35037. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M95580 Genomic DNA. Translation: AAA35037.1.
Z36018 Genomic DNA. Translation: CAA85107.1.
BK006936 Genomic DNA. Translation: DAA07264.1.
PIRiS46020.
RefSeqiNP_009707.3. NM_001178497.3.

Genome annotation databases

EnsemblFungiiYBR149W; YBR149W; YBR149W.
GeneIDi852446.
KEGGisce:YBR149W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M95580 Genomic DNA. Translation: AAA35037.1 .
Z36018 Genomic DNA. Translation: CAA85107.1 .
BK006936 Genomic DNA. Translation: DAA07264.1 .
PIRi S46020.
RefSeqi NP_009707.3. NM_001178497.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4IJC X-ray 2.10 A/B 1-344 [» ]
4IJR X-ray 2.00 A/C 1-344 [» ]
ProteinModelPortali P38115.
SMRi P38115. Positions 15-342.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32848. 21 interactions.
DIPi DIP-4926N.
MINTi MINT-521486.
STRINGi 4932.YBR149W.

Proteomic databases

MaxQBi P38115.
PaxDbi P38115.
PeptideAtlasi P38115.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR149W ; YBR149W ; YBR149W .
GeneIDi 852446.
KEGGi sce:YBR149W.

Organism-specific databases

CYGDi YBR149w.
SGDi S000000353. ARA1.

Phylogenomic databases

eggNOGi COG0656.
GeneTreei ENSGT00750000117803.
HOGENOMi HOG000250272.
KOi K00063.
OrthoDBi EOG7WQ83S.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER3O-32366.
MetaCyc:MONOMER3O-32367.
YEAST:MONOMER3O-32366.
YEAST:MONOMER3O-32367.
Reactomei REACT_189323. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
REACT_94166. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.

Miscellaneous databases

NextBioi 971354.

Gene expression databases

Genevestigatori P38115.

Family and domain databases

Gene3Di 3.20.20.100. 1 hit.
InterProi IPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view ]
PANTHERi PTHR11732. PTHR11732. 1 hit.
Pfami PF00248. Aldo_ket_red. 1 hit.
[Graphical view ]
PIRSFi PIRSF000097. AKR. 1 hit.
PRINTSi PR00069. ALDKETRDTASE.
SUPFAMi SSF51430. SSF51430. 1 hit.
PROSITEi PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A widely distributed 'CAT' family of repetitive DNA sequences."
    Martinez-Soriano J.P., Wong W.M., van Ryk D.I., Nazar R.N.
    J. Mol. Biol. 217:629-635(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "D-arabinose dehydrogenase and its gene from Saccharomyces cerevisiae."
    Kim S.T., Huh W.K., Lee B.H., Kang S.O.
    Biochim. Biophys. Acta 1429:29-39(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 7-20.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiARA1_YEAST
AccessioniPrimary (citable) accession number: P38115
Secondary accession number(s): D6VQE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 3, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 30200 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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