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Protein

D-arabinose dehydrogenase [NAD(P)+] heavy chain

Gene

ARA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of D-arabinose, L-xylose, L-fucose and L-galactose in the presence of NADP+.

Miscellaneous

Present with 30200 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

D-arabinose + NAD(P)+ = D-arabinono-1,4-lactone + NAD(P)H.

pH dependencei

Optimum pH is 10.0.

Temperature dependencei

Optimum temperature is about 30 degrees Celsius.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei71Proton donorBy similarity1
Sitei100Lowers pKa of active site TyrBy similarity1
Binding sitei131SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi241 – 295NADPBy similarityAdd BLAST55

GO - Molecular functioni

  • D-arabinose 1-dehydrogenase [NAD(P)+] activity Source: SGD

GO - Biological processi

  • cellular carbohydrate metabolic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-20236
YEAST:MONOMER3O-32366
YEAST:MONOMER3O-32367

Names & Taxonomyi

Protein namesi
Recommended name:
D-arabinose dehydrogenase [NAD(P)+] heavy chain (EC:1.1.1.117)
Alternative name(s):
AKR3C
Gene namesi
Name:ARA1
Ordered Locus Names:YBR149W
ORF Names:YBR1127
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR149W
SGDiS000000353 ARA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001246101 – 344D-arabinose dehydrogenase [NAD(P)+] heavy chainAdd BLAST344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei151PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38115
PaxDbiP38115
PRIDEiP38115

PTM databases

iPTMnetiP38115

Interactioni

Subunit structurei

Heterodimer of a heavy chain and a light chain.

Protein-protein interaction databases

BioGridi32848, 71 interactors
DIPiDIP-4926N
IntActiP38115, 12 interactors
MINTiP38115
STRINGi4932.YBR149W

Structurei

Secondary structure

1344
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi20 – 22Combined sources3
Beta strandi23 – 26Combined sources4
Beta strandi32 – 36Combined sources5
Helixi44 – 49Combined sources6
Helixi50 – 59Combined sources10
Beta strandi64 – 66Combined sources3
Helixi69 – 71Combined sources3
Helixi74 – 86Combined sources13
Helixi92 – 94Combined sources3
Beta strandi96 – 101Combined sources6
Helixi103 – 105Combined sources3
Helixi109 – 120Combined sources12
Beta strandi126 – 130Combined sources5
Helixi167 – 179Combined sources13
Beta strandi185 – 187Combined sources3
Beta strandi189 – 193Combined sources5
Helixi196 – 205Combined sources10
Beta strandi211 – 216Combined sources6
Helixi224 – 233Combined sources10
Beta strandi236 – 241Combined sources6
Helixi249 – 252Combined sources4
Helixi254 – 262Combined sources9
Helixi267 – 277Combined sources11
Helixi289 – 294Combined sources6
Helixi303 – 315Combined sources13
Helixi324 – 326Combined sources3
Helixi339 – 341Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4IJCX-ray2.10A/B1-344[»]
4IJRX-ray2.00A/C1-344[»]
ProteinModelPortaliP38115
SMRiP38115
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

GeneTreeiENSGT00850000132445
HOGENOMiHOG000250272
InParanoidiP38115
KOiK00063
OMAiTWQAKPE
OrthoDBiEOG092C324N

Family and domain databases

CDDicd06660 Aldo_ket_red, 1 hit
Gene3Di3.20.20.100, 1 hit
InterProiView protein in InterPro
IPR018170 Aldo/ket_reductase_CS
IPR020471 Aldo/keto_reductase
IPR023210 NADP_OxRdtase_dom
IPR036812 NADP_OxRdtase_dom_sf
PANTHERiPTHR11732 PTHR11732, 1 hit
PfamiView protein in Pfam
PF00248 Aldo_ket_red, 1 hit
PIRSFiPIRSF000097 AKR, 1 hit
PRINTSiPR00069 ALDKETRDTASE
SUPFAMiSSF51430 SSF51430, 1 hit
PROSITEiView protein in PROSITE
PS00798 ALDOKETO_REDUCTASE_1, 1 hit
PS00062 ALDOKETO_REDUCTASE_2, 1 hit
PS00063 ALDOKETO_REDUCTASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

P38115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSVASTEN IVENMLHPKT TEIYFSLNNG VRIPALGLGT ANPHEKLAET
60 70 80 90 100
KQAVKAAIKA GYRHIDTAWA YETEPFVGEA IKELLEDGSI KREDLFITTK
110 120 130 140 150
VWPVLWDEVD RSLNESLKAL GLEYVDLLLQ HWPLCFEKIK DPKGISGLVK
160 170 180 190 200
TPVDDSGKTM YAADGDYLET YKQLEKIYLD PNDHRVRAIG VSNFSIEYLE
210 220 230 240 250
RLIKECRVKP TVNQVETHPH LPQMELRKFC FMHDILLTAY SPLGSHGAPN
260 270 280 290 300
LKIPLVKKLA EKYNVTGNDL LISYHIRQGT IVIPRSLNPV RISSSIEFAS
310 320 330 340
LTKDELQELN DFGEKYPVRF IDEPFAAILP EFTGNGPNLD NLKY
Length:344
Mass (Da):38,884
Last modified:October 1, 1994 - v1
Checksum:iF9B6D9333B18FEEC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56 – 62AAIKAGY → LQSKLDN in AAA35037 (PubMed:2005616).Curated7
Sequence conflicti71 – 73YET → SR in AAA35037 (PubMed:2005616).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95580 Genomic DNA Translation: AAA35037.1
Z36018 Genomic DNA Translation: CAA85107.1
BK006936 Genomic DNA Translation: DAA07264.1
PIRiS46020
RefSeqiNP_009707.3, NM_001178497.3

Genome annotation databases

EnsemblFungiiYBR149W; YBR149W; YBR149W
GeneIDi852446
KEGGisce:YBR149W

Similar proteinsi

Entry informationi

Entry nameiARA1_YEAST
AccessioniPrimary (citable) accession number: P38115
Secondary accession number(s): D6VQE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: March 28, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health