Reviewed, UniProtKB/Swiss-Prot P38113 (ADH5_YEAST)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase 5
    EC=1.1.1.1
Alternative name(s):
    Alcohol dehydrogenase V

Gene names

Name:	ADH5
Ordered Locus Names:	YBR145W
ORF Names:	YBR1122

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Miscellaneous	Present with 1310 molecules/cell in log phase SD medium. Ref.2
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NADH oxidation Inferred from mutant phenotype. Source: SGD amino acid catabolic process to alcohol via Ehrlich pathway Inferred from genetic interaction. Source: SGD ethanol biosynthetic process during fermentation Inferred from mutant phenotype. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	alcohol dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARO8	P53090	1	EBI-2239,EBI-2042933
SAM50	P53969	1	EBI-2239,EBI-28646
UBA1	P22515	1	EBI-2239,EBI-19703

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Alcohol dehydrogenase 5		PRO_0000160733
Regions
	Nucleotide binding	181 – 187	7	NAD By similarity
	Nucleotide binding	272 – 274	3	NAD By similarity
Sites
	Metal binding	47	1	Zinc 1; catalytic By similarity
	Metal binding	70	1	Zinc 1; catalytic By similarity
	Metal binding	101	1	Zinc 2 By similarity
	Metal binding	104	1	Zinc 2 By similarity
	Metal binding	107	1	Zinc 2 By similarity
	Metal binding	115	1	Zinc 2 By similarity
	Metal binding	183	1	Zinc 1; catalytic By similarity
	Binding site	205	1	NAD By similarity
	Binding site	210	1	NAD By similarity
	Binding site	344	1	NAD By similarity
Amino acid modifications
	Modified residue	252	1	Phosphoserine Ref.4
	Modified residue	305	1	Phosphothreonine Ref.4 Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P38113-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: F4084DAD1E3C19DE	FASTA	351	37,648
	10 20 30 40 50 60 MPSQVIPEKQ KAIVFYETDG KLEYKDVTVP EPKPNEILVH VKYSGVCHSD LHAWHGDWPF 70 80 90 100 110 120 QLKFPLIGGH EGAGVVVKLG SNVKGWKVGD FAGIKWLNGT CMSCEYCEVG NESQCPYLDG 130 140 150 160 170 180 TGFTHDGTFQ EYATADAVQA AHIPPNVNLA EVAPILCAGI TVYKALKRAN VIPGQWVTIS 190 200 210 220 230 240 GACGGLGSLA IQYALAMGYR VIGIDGGNAK RKLFEQLGGE IFIDFTEEKD IVGAIIKATN 250 260 270 280 290 300 GGSHGVINVS VSEAAIEAST RYCRPNGTVV LVGMPAHAYC NSDVFNQVVK SISIVGSCVG 310 320 330 340 350 NRADTREALD FFARGLIKSP IHLAGLSDVP EIFAKMEKGE IVGRYVVETS K

References

[1]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[3]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-305, MASS SPECTROMETRY.
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-252 AND THR-305, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36014 Genomic DNA. Translation: CAA85103.1.
PIR	S46016.
RefSeq	NP_009703.1.
3D structure databases
SMR	P38113. Positions 6-351.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4500N.
IntAct	P38113. 7 interactions.
STRING	P38113.
Proteomic databases
PeptideAtlas	P38113.
PRIDE	P38113.
Genome annotation databases
Ensembl	YBR145W; YBR145W; YBR145W; Saccharomyces cerevisiae. [Genome view]
GeneID	852442.
KEGG	sce:YBR145W.
NMPDR	fig|4932.3.peg.409.
Organism-specific databases
CYGD	YBR145w.
SGD	S000000349. ADH5.
Phylogenomic databases
eggNOG	fuNOG04605.
HOGENOM	HBG753318.
OMA	NARNEDS.
OrthoDB	EOG95HTDV.
PhylomeDB	P38113.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11839.
BRENDA	1.1.1.1. 250.
Gene expression databases
ArrayExpress	P38113.
Genevestigator	P38113.
GermOnline	YBR145W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	971342.

Entry information

Entry name

ADH5_YEAST

Accession

Primary (citable) accession number: P38113

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	January 19, 2010
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)