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Protein

ATP-dependent RNA helicase MAK5

Gene

MAK5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs. Required for the maintenance of dsRNA killer plasmid.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi215 – 2228ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
  • RNA secondary structure unwinding Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29096-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase MAK5 (EC:3.6.4.13)
Alternative name(s):
Maintenance of killer protein 5
Gene namesi
Name:MAK5
Ordered Locus Names:YBR142W
ORF Names:YBR1119
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR142W.
SGDiS000000346. MAK5.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi218 – 2181G → D: Delays the appearance and decreases the level of 25S rRNA. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 773773ATP-dependent RNA helicase MAK5PRO_0000055048Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei135 – 1351PhosphothreonineCombined sources
Modified residuei138 – 1381PhosphoserineCombined sources
Modified residuei678 – 6781PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38112.

PTM databases

iPTMnetiP38112.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
DBP10Q123893EBI-10394,EBI-5644
HAS1Q035325EBI-10394,EBI-8170
LOC1P435863EBI-10394,EBI-22906
NOP12Q082084EBI-10394,EBI-35895
NOP4P378385EBI-10394,EBI-12122
RLP7P406934EBI-10394,EBI-15415
RPF1P388053EBI-10394,EBI-24614

Protein-protein interaction databases

BioGridi32842. 87 interactions.
DIPiDIP-6337N.
IntActiP38112. 59 interactions.
MINTiMINT-612928.

Structurei

3D structure databases

ProteinModelPortaliP38112.
SMRiP38112. Positions 150-587.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini202 – 399198Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini452 – 615164Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi171 – 19929Q motifAdd
BLAST
Motifi333 – 3364DEAD box

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074847.
HOGENOMiHOG000290702.
InParanoidiP38112.
KOiK14805.
OMAiIECGAME.
OrthoDBiEOG7NW6JS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKRAPQKG KTVTKPQEII VDESKLNWKP VDIPDTLDDF GGFYGLEEID
60 70 80 90 100
GVDVKVVDGK VTFVTKKDSK VLKDSNKEKV GDDQESVENE SGSDSESELL
110 120 130 140 150
EFKNLDDIKE GELSAASYSS SDEDEQGNIE SSKLTDPSED VDEDVDEDVL
160 170 180 190 200
KENVFNKDIN IDDISPVNLP EWTNLAPLSM TILQSLQNLN FLRPTEIQKK
210 220 230 240 250
SIPVIMQGVD VMGKASTGSG KTLAYGIPIV EKLISNFSQK NKKPISLIFT
260 270 280 290 300
PTRELAHQVT DHLKKICEPV LAKSQYSILS LTGGLSIQKQ QRLLKYDNSG
310 320 330 340 350
QIVIATPGRF LELLEKDNTL IKRFSKVNTL ILDEADRLLQ DGHFDEFEKI
360 370 380 390 400
IKHLLVERRK NRENSEGSSK IWQTLIFSAT FSIDLFDKLS SSRQVKDRRF
410 420 430 440 450
KNNEDELNAV IQHLMSKIHF NSKPVIIDTN PESKVSSQIK ESLIECPPLE
460 470 480 490 500
RDLYCYYFLT MFPGTTLIFC NAIDSVKKLT VYLNNLGIPA FQIHSSMTQK
510 520 530 540 550
NRLKSLERFK QQSAKQKTIN HSNPDSVQLS TVLIASDVAA RGLDIPGVQH
560 570 580 590 600
VIHYHLPRST DIYIHRSGRT ARAGSEGVSA MICSPQESMG PLRKLRKTLA
610 620 630 640 650
TKNSVSTDLN SRSTNRKPIK WQNTVPLLPI ETDILSQLRE RSRLAGELAD
660 670 680 690 700
HEIASNSLRK DDNWLKKAAD ELGIDVDSDE DDISKSNSDT FLLKNKNKKM
710 720 730 740 750
QKTINKDKVK AMRATLNELL SVPIRKDRRQ KYLTGGLVNL ADNLVKKRGH
760 770
NSIIGHEKTN ALETLKKKKK RNN
Length:773
Mass (Da):87,048
Last modified:October 1, 1994 - v1
Checksum:iC4FF2FB5B04FFBF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36011 Genomic DNA. Translation: CAA85100.1.
X78937 Genomic DNA. Translation: CAA55539.1.
BK006936 Genomic DNA. Translation: DAA07258.1.
PIRiS46011.
RefSeqiNP_009700.1. NM_001178490.1.

Genome annotation databases

EnsemblFungiiYBR142W; YBR142W; YBR142W.
GeneIDi852439.
KEGGisce:YBR142W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36011 Genomic DNA. Translation: CAA85100.1.
X78937 Genomic DNA. Translation: CAA55539.1.
BK006936 Genomic DNA. Translation: DAA07258.1.
PIRiS46011.
RefSeqiNP_009700.1. NM_001178490.1.

3D structure databases

ProteinModelPortaliP38112.
SMRiP38112. Positions 150-587.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32842. 87 interactions.
DIPiDIP-6337N.
IntActiP38112. 59 interactions.
MINTiMINT-612928.

PTM databases

iPTMnetiP38112.

Proteomic databases

MaxQBiP38112.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR142W; YBR142W; YBR142W.
GeneIDi852439.
KEGGisce:YBR142W.

Organism-specific databases

EuPathDBiFungiDB:YBR142W.
SGDiS000000346. MAK5.

Phylogenomic databases

GeneTreeiENSGT00550000074847.
HOGENOMiHOG000290702.
InParanoidiP38112.
KOiK14805.
OMAiIECGAME.
OrthoDBiEOG7NW6JS.

Enzyme and pathway databases

BioCyciYEAST:G3O-29096-MONOMER.

Miscellaneous databases

PROiP38112.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of 12.5 kb from the right arm of chromosome II predicts a new N-terminal sequence for the IRA1 protein and reveals two new genes, one of which is a DEAD-box helicase."
    Zagulski M., Becam A.-M., Grzybowska E., Lacroute F., Migdalski A., Slonimski P.P., Sokolowska B., Herbert C.J.
    Yeast 10:1227-1234(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: the killer character of yeast."
    Wickner R.B., Leibowitz M.J.
    J. Mol. Biol. 105:427-443(1976) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Mak5p, which is required for the maintenance of the M1 dsRNA virus, is encoded by the yeast ORF YBR142w and is involved in the biogenesis of the 60S subunit of the ribosome."
    Zagulski M., Kressler D., Becam A.-M., Rytka J., Herbert C.J.
    Mol. Genet. Genomics 270:216-224(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-218.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-135 AND SER-678, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-138 AND SER-678, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-135; SER-138 AND SER-678, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMAK5_YEAST
AccessioniPrimary (citable) accession number: P38112
Secondary accession number(s): D6VQD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 6, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 981 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.