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Protein

ATP-dependent RNA helicase MAK5

Gene

MAK5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs. Required for the maintenance of dsRNA killer plasmid.2 Publications

Miscellaneous

Present with 981 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi215 – 222ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal large subunit biogenesis Source: SGD
  • RNA secondary structure unwinding Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processRibosome biogenesis, rRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29096-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase MAK5 (EC:3.6.4.13)
Alternative name(s):
Maintenance of killer protein 5
Gene namesi
Name:MAK5
Ordered Locus Names:YBR142W
ORF Names:YBR1119
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR142W
SGDiS000000346 MAK5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi218G → D: Delays the appearance and decreases the level of 25S rRNA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550481 – 773ATP-dependent RNA helicase MAK5Add BLAST773

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135PhosphothreonineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei678PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38112
PaxDbiP38112
PRIDEiP38112

PTM databases

iPTMnetiP38112

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32842, 320 interactors
DIPiDIP-6337N
IntActiP38112, 60 interactors
MINTiP38112
STRINGi4932.YBR142W

Structurei

3D structure databases

ProteinModelPortaliP38112
SMRiP38112
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini202 – 399Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST198
Domaini452 – 615Helicase C-terminalPROSITE-ProRule annotationAdd BLAST164

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi171 – 199Q motifAdd BLAST29
Motifi333 – 336DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074847
HOGENOMiHOG000290702
InParanoidiP38112
KOiK14805
OMAiYYFLLRY
OrthoDBiEOG092C1I7Y

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

P38112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKRAPQKG KTVTKPQEII VDESKLNWKP VDIPDTLDDF GGFYGLEEID
60 70 80 90 100
GVDVKVVDGK VTFVTKKDSK VLKDSNKEKV GDDQESVENE SGSDSESELL
110 120 130 140 150
EFKNLDDIKE GELSAASYSS SDEDEQGNIE SSKLTDPSED VDEDVDEDVL
160 170 180 190 200
KENVFNKDIN IDDISPVNLP EWTNLAPLSM TILQSLQNLN FLRPTEIQKK
210 220 230 240 250
SIPVIMQGVD VMGKASTGSG KTLAYGIPIV EKLISNFSQK NKKPISLIFT
260 270 280 290 300
PTRELAHQVT DHLKKICEPV LAKSQYSILS LTGGLSIQKQ QRLLKYDNSG
310 320 330 340 350
QIVIATPGRF LELLEKDNTL IKRFSKVNTL ILDEADRLLQ DGHFDEFEKI
360 370 380 390 400
IKHLLVERRK NRENSEGSSK IWQTLIFSAT FSIDLFDKLS SSRQVKDRRF
410 420 430 440 450
KNNEDELNAV IQHLMSKIHF NSKPVIIDTN PESKVSSQIK ESLIECPPLE
460 470 480 490 500
RDLYCYYFLT MFPGTTLIFC NAIDSVKKLT VYLNNLGIPA FQIHSSMTQK
510 520 530 540 550
NRLKSLERFK QQSAKQKTIN HSNPDSVQLS TVLIASDVAA RGLDIPGVQH
560 570 580 590 600
VIHYHLPRST DIYIHRSGRT ARAGSEGVSA MICSPQESMG PLRKLRKTLA
610 620 630 640 650
TKNSVSTDLN SRSTNRKPIK WQNTVPLLPI ETDILSQLRE RSRLAGELAD
660 670 680 690 700
HEIASNSLRK DDNWLKKAAD ELGIDVDSDE DDISKSNSDT FLLKNKNKKM
710 720 730 740 750
QKTINKDKVK AMRATLNELL SVPIRKDRRQ KYLTGGLVNL ADNLVKKRGH
760 770
NSIIGHEKTN ALETLKKKKK RNN
Length:773
Mass (Da):87,048
Last modified:October 1, 1994 - v1
Checksum:iC4FF2FB5B04FFBF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36011 Genomic DNA Translation: CAA85100.1
X78937 Genomic DNA Translation: CAA55539.1
BK006936 Genomic DNA Translation: DAA07258.1
PIRiS46011
RefSeqiNP_009700.1, NM_001178490.1

Genome annotation databases

EnsemblFungiiYBR142W; YBR142W; YBR142W
GeneIDi852439
KEGGisce:YBR142W

Similar proteinsi

Entry informationi

Entry nameiMAK5_YEAST
AccessioniPrimary (citable) accession number: P38112
Secondary accession number(s): D6VQD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 23, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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