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Protein

Probable diguanylate cyclase DgcE

Gene

dgcE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the synthesis of cyclic-di-GMP (c-di-GMP) via the condensation of 2 GTP molecules (By similarity). Involved in the control of the switch from cell motility to adhesion via regulation of cellular levels of c-di-GMP (Probable). Part of a signaling cascade that regulates curli biosynthesis. The cascade is composed of two c-di-GMP control modules, in which c-di-GMP controlled by the DgcE/PdeH pair (module I) regulates the activity of the DgcM/PdeR pair (module II), which in turn regulates activity of the transcription factor MlrA and expression of the master biofilm regulator csgD (PubMed:23708798).By similarity1 Publication1 Publication

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.Curated
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi720MagnesiumBy similarity1
Sitei725Transition state stabilizerSequence analysis1
Binding sitei728SubstrateBy similarity1
Binding sitei733Substrate; via carbonyl oxygenBy similarity1
Binding sitei737SubstrateBy similarity1
Active sitei763Proton acceptorSequence analysis1
Metal bindingi763MagnesiumBy similarity1
Binding sitei783SubstrateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12396-MONOMER
UniPathwayiUPA00599

Names & Taxonomyi

Protein namesi
Recommended name:
Probable diguanylate cyclase DgcECurated (EC:2.7.7.65By similarity)
Short name:
DGCCurated
Gene namesi
Name:dgcE1 Publication
Synonyms:yegE
Ordered Locus Names:b2067, JW2052
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG12396 yegE

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Transmembranei64 – 84HelicalSequence analysisAdd BLAST21
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
Transmembranei156 – 176HelicalSequence analysisAdd BLAST21
Transmembranei190 – 207HelicalSequence analysisAdd BLAST18
Transmembranei211 – 228HelicalSequence analysisAdd BLAST18
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Transmembranei270 – 290HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Decreased biofilm formation, decreased expression of DgcC, a probable diguanylate cyclase and of the curli regulator CsgD (PubMed:19332833). Disruption leads to decreaseded expression of adhesive curli fimbriae genes (PubMed:18765794). Also partially suppresses the reduced motility of a pdeH disruption; concomitant disruption of dosC, dgcE, dgcQ and dgcN completely restores motility, suggesting these 4 genes, together with the c-di-GMP phosphodiesterase PdeH, form a network that regulates cell motility by altering levels of c-di-GMP.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001691221 – 1105Probable diguanylate cyclase DgcEAdd BLAST1105

Proteomic databases

PaxDbiP38097
PRIDEiP38097

Expressioni

Inductioni

Expressed during transition into stationary phase, expression is equal at 28 and 37 degrees Celsius. Expression is RpoS dependent.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4259682, 200 interactors
IntActiP38097, 1 interactor
STRINGi316385.ECDH10B_2217

Structurei

3D structure databases

ProteinModelPortaliP38097
SMRiP38097
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini300 – 370PAS 1PROSITE-ProRule annotationAdd BLAST71
Domaini374 – 426PAC 1PROSITE-ProRule annotationAdd BLAST53
Domaini501 – 552PAC 2PROSITE-ProRule annotationAdd BLAST52
Domaini553 – 623PAS 2PROSITE-ProRule annotationAdd BLAST71
Domaini626 – 680PAC 3PROSITE-ProRule annotationAdd BLAST55
Domaini712 – 845GGDEFPROSITE-ProRule annotationAdd BLAST134
Domaini855 – 1104EALPROSITE-ProRule annotationAdd BLAST250

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU Bacteria
COG2202 LUCA
COG3447 LUCA
HOGENOMiHOG000122009
InParanoidiP38097
KOiK21084
OMAiIVYTHMT
PhylomeDBiP38097

Family and domain databases

CDDicd01949 GGDEF, 1 hit
cd00130 PAS, 3 hits
Gene3Di3.20.20.450, 1 hit
InterProiView protein in InterPro
IPR001633 EAL_dom
IPR035919 EAL_sf
IPR000160 GGDEF_dom
IPR007895 MASE1
IPR029787 Nucleotide_cyclase
IPR001610 PAC
IPR000014 PAS
IPR000700 PAS-assoc_C
IPR035965 PAS-like_dom_sf
IPR013656 PAS_4
IPR013655 PAS_fold_3
PfamiView protein in Pfam
PF00563 EAL, 1 hit
PF00990 GGDEF, 1 hit
PF05231 MASE1, 1 hit
PF08447 PAS_3, 1 hit
PF08448 PAS_4, 1 hit
PF13426 PAS_9, 1 hit
SMARTiView protein in SMART
SM00052 EAL, 1 hit
SM00267 GGDEF, 1 hit
SM00086 PAC, 3 hits
SM00091 PAS, 3 hits
SUPFAMiSSF141868 SSF141868, 1 hit
SSF55073 SSF55073, 1 hit
SSF55785 SSF55785, 3 hits
TIGRFAMsiTIGR00254 GGDEF, 1 hit
TIGR00229 sensory_box, 2 hits
PROSITEiView protein in PROSITE
PS50883 EAL, 1 hit
PS50887 GGDEF, 1 hit
PS50113 PAC, 3 hits
PS50112 PAS, 2 hits

Sequencei

Sequence statusi: Complete.

P38097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKQSQHVLI ALPHPLLHLV SLGLVSFIFT LFSLELSQFG TQLAPLWFPT
60 70 80 90 100
SIMMVAFYRH AGRMWPGIAL SCSLGNIAAS ILLFSTSSLN MTWTTINIVE
110 120 130 140 150
AVVGAVLLRK LLPWYNPLQN LADWLRLALG SAIVPPLLGG VLVVLLTPGD
160 170 180 190 200
DPLRAFLIWV LSESIGALAL VPLGLLFKPH YLLRHRNPRL LFESLLTLAI
210 220 230 240 250
TLTLSWLSML YLPWPFTFII VLLMWSAVRL PRMEAFLIFL TTVMMVSLMM
260 270 280 290 300
AADPSLLATP RTYLMSHMPW LPFLLILLPA NIMTMVMYAF RAERKHISES
310 320 330 340 350
ETHFRNAMEY SAIGMALVGT EGQWLQTNKA LCQFLGYSQE ELRGLTFQQL
360 370 380 390 400
TWPEDLNKDL QQVEKLISGE INTYSMEKRY YNRNGDVVWA LLAVSLVRHT
410 420 430 440 450
DGTPLYFIAQ IEDINELKRT EQVNQQLMER ITLANEAGGI GIWEWELKPN
460 470 480 490 500
IFSWDKRMFE LYEIPPHIKP NWQVWYECVL PEDRQHAEKV IRDSLQSRSP
510 520 530 540 550
FKLEFRITVK DGIRHIRALA NRVLNKEGEV ERLLGINMDM TEVKQLNEAL
560 570 580 590 600
FQEKERLHIT LDSIGEAVVC IDMAMKITFM NPVAEKMSGW TQEEALGVPL
610 620 630 640 650
LTVLHITFGD NGPLMENIYS ADTSRSAIEQ DVVLHCRSGG SYDVHYSITP
660 670 680 690 700
LSTLDGSNIG SVLVIQDVTE SRKMLRQLSY SASHDALTHL ANRASFEKQL
710 720 730 740 750
RILLQTVNST HQRHALVFID LDRFKAVNDS AGHAAGDALL RELASLMLSM
760 770 780 790 800
LRSSDVLARL GGDEFGLLLP DCNVESARFI ATRIISAVND YHFIWEGRVH
810 820 830 840 850
RVGASAGITL IDDNNHQAAE VMSQADIACY ASKNGGRGRV TVYEPQQAAA
860 870 880 890 900
HSERAAMSLD EQWRMIKENQ LMMLAHGVAS PRIPEARNLW LISLKLWSCE
910 920 930 940 950
GEIIDEQTFR RSFSDPALSH ALDRRVFHEF FQQAAKAVAS KGISISLPLS
960 970 980 990 1000
VAGLSSATLV NDLLEQLENS PLPPRLLHLI IPAEAILDHA ESVQKLRLAG
1010 1020 1030 1040 1050
CRIVLSQVGR DLQIFNSLKA NMADYLLLDG ELCANVQGNL MDEMLITIIQ
1060 1070 1080 1090 1100
GHAQRLGMKT IAGPVVLPLV MDTLSGIGVD LIYGEVIADA QPLDLLVNSS

YFAIN
Length:1,105
Mass (Da):123,887
Last modified:November 1, 1997 - v2
Checksum:i22BE64B963CB9739
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti965 – 966EQ → NS in K02498 (PubMed:6094528).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75128.1
AP009048 Genomic DNA Translation: BAA15920.1
K02498 Genomic DNA No translation available.
PIRiB64973
RefSeqiNP_416571.1, NC_000913.3
WP_000043761.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75128; AAC75128; b2067
BAA15920; BAA15920; BAA15920
GeneIDi946600
KEGGiecj:JW2052
eco:b2067
PATRICifig|1411691.4.peg.184

Similar proteinsi

Entry informationi

Entry nameiDGCE_ECOLI
AccessioniPrimary (citable) accession number: P38097
Secondary accession number(s): P76391
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: April 25, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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