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Protein

Probable diguanylate cyclase YegE

Gene

yegE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.1 Publication

Catalytic activityi

2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per monomer.By similarity

Pathwayi: 3',5'-cyclic di-GMP biosynthesis

This protein is involved in the pathway 3',5'-cyclic di-GMP biosynthesis, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway 3',5'-cyclic di-GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi720MagnesiumBy similarity1
Sitei725Transition state stabilizerSequence analysis1
Binding sitei728SubstrateBy similarity1
Binding sitei737SubstrateBy similarity1
Active sitei763Proton acceptorSequence analysis1
Metal bindingi763MagnesiumBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12396-MONOMER.
ECOL316407:JW2052-MONOMER.
UniPathwayiUPA00599.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable diguanylate cyclase YegE (EC:2.7.7.65)
Short name:
DGC
Gene namesi
Name:yegE
Ordered Locus Names:b2067, JW2052
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12396. yegE.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Decreased biofilm formation, decreased expression of AdrA, a probable diguanylate cyclase and of the curli regulator CsgD (PubMed:19332833). Disruption leads to decreaseded expression of adhesive curli fimbriae genes (PubMed:18765794). Also partially suppresses the reduced motility of a yhjH disruption; concomitant disruption of dosC, yegE, yedQ and yfiN completely restores motility, suggesting these 4 genes, together with the c-di-GMP phosphodiesterase YhjH, form a network that regulates cell motility by altering levels of c-di-GMP.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001691221 – 1105Probable diguanylate cyclase YegEAdd BLAST1105

Proteomic databases

PaxDbiP38097.
PRIDEiP38097.

Expressioni

Inductioni

Expressed during transition into stationary phase, expression is equal at 28 and 37 degrees Celsius. Expression is RpoS dependent.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4259682. 183 interactors.
IntActiP38097. 1 interactor.
STRINGi511145.b2067.

Structurei

3D structure databases

ProteinModelPortaliP38097.
SMRiP38097.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini300 – 370PAS 1PROSITE-ProRule annotationAdd BLAST71
Domaini374 – 426PAC 1PROSITE-ProRule annotationAdd BLAST53
Domaini501 – 552PAC 2PROSITE-ProRule annotationAdd BLAST52
Domaini553 – 623PAS 2PROSITE-ProRule annotationAdd BLAST71
Domaini626 – 680PAC 3PROSITE-ProRule annotationAdd BLAST55
Domaini712 – 845GGDEFPROSITE-ProRule annotationAdd BLAST134
Domaini855 – 1104EALPROSITE-ProRule annotationAdd BLAST250

Sequence similaritiesi

Contains 1 EAL domain.PROSITE-ProRule annotation
Contains 1 GGDEF domain.PROSITE-ProRule annotation
Contains 3 PAC (PAS-associated C-terminal) domains.PROSITE-ProRule annotation
Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG2202. LUCA.
COG3447. LUCA.
HOGENOMiHOG000122009.
InParanoidiP38097.
OMAiIRIDERF.
PhylomeDBiP38097.

Family and domain databases

CDDicd01949. GGDEF. 1 hit.
Gene3Di3.20.20.450. 1 hit.
InterProiIPR001633. EAL_dom.
IPR000160. GGDEF_dom.
IPR007895. MASE1.
IPR029787. Nucleotide_cyclase.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013656. PAS_4.
IPR013655. PAS_fold_3.
[Graphical view]
PfamiPF00563. EAL. 1 hit.
PF00990. GGDEF. 1 hit.
PF05231. MASE1. 1 hit.
PF08447. PAS_3. 1 hit.
PF08448. PAS_4. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
SMARTiSM00052. EAL. 1 hit.
SM00267. GGDEF. 1 hit.
SM00086. PAC. 3 hits.
SM00091. PAS. 3 hits.
[Graphical view]
SUPFAMiSSF141868. SSF141868. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF55785. SSF55785. 3 hits.
TIGRFAMsiTIGR00254. GGDEF. 1 hit.
TIGR00229. sensory_box. 2 hits.
PROSITEiPS50883. EAL. 1 hit.
PS50887. GGDEF. 1 hit.
PS50113. PAC. 3 hits.
PS50112. PAS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKQSQHVLI ALPHPLLHLV SLGLVSFIFT LFSLELSQFG TQLAPLWFPT
60 70 80 90 100
SIMMVAFYRH AGRMWPGIAL SCSLGNIAAS ILLFSTSSLN MTWTTINIVE
110 120 130 140 150
AVVGAVLLRK LLPWYNPLQN LADWLRLALG SAIVPPLLGG VLVVLLTPGD
160 170 180 190 200
DPLRAFLIWV LSESIGALAL VPLGLLFKPH YLLRHRNPRL LFESLLTLAI
210 220 230 240 250
TLTLSWLSML YLPWPFTFII VLLMWSAVRL PRMEAFLIFL TTVMMVSLMM
260 270 280 290 300
AADPSLLATP RTYLMSHMPW LPFLLILLPA NIMTMVMYAF RAERKHISES
310 320 330 340 350
ETHFRNAMEY SAIGMALVGT EGQWLQTNKA LCQFLGYSQE ELRGLTFQQL
360 370 380 390 400
TWPEDLNKDL QQVEKLISGE INTYSMEKRY YNRNGDVVWA LLAVSLVRHT
410 420 430 440 450
DGTPLYFIAQ IEDINELKRT EQVNQQLMER ITLANEAGGI GIWEWELKPN
460 470 480 490 500
IFSWDKRMFE LYEIPPHIKP NWQVWYECVL PEDRQHAEKV IRDSLQSRSP
510 520 530 540 550
FKLEFRITVK DGIRHIRALA NRVLNKEGEV ERLLGINMDM TEVKQLNEAL
560 570 580 590 600
FQEKERLHIT LDSIGEAVVC IDMAMKITFM NPVAEKMSGW TQEEALGVPL
610 620 630 640 650
LTVLHITFGD NGPLMENIYS ADTSRSAIEQ DVVLHCRSGG SYDVHYSITP
660 670 680 690 700
LSTLDGSNIG SVLVIQDVTE SRKMLRQLSY SASHDALTHL ANRASFEKQL
710 720 730 740 750
RILLQTVNST HQRHALVFID LDRFKAVNDS AGHAAGDALL RELASLMLSM
760 770 780 790 800
LRSSDVLARL GGDEFGLLLP DCNVESARFI ATRIISAVND YHFIWEGRVH
810 820 830 840 850
RVGASAGITL IDDNNHQAAE VMSQADIACY ASKNGGRGRV TVYEPQQAAA
860 870 880 890 900
HSERAAMSLD EQWRMIKENQ LMMLAHGVAS PRIPEARNLW LISLKLWSCE
910 920 930 940 950
GEIIDEQTFR RSFSDPALSH ALDRRVFHEF FQQAAKAVAS KGISISLPLS
960 970 980 990 1000
VAGLSSATLV NDLLEQLENS PLPPRLLHLI IPAEAILDHA ESVQKLRLAG
1010 1020 1030 1040 1050
CRIVLSQVGR DLQIFNSLKA NMADYLLLDG ELCANVQGNL MDEMLITIIQ
1060 1070 1080 1090 1100
GHAQRLGMKT IAGPVVLPLV MDTLSGIGVD LIYGEVIADA QPLDLLVNSS

YFAIN
Length:1,105
Mass (Da):123,887
Last modified:November 1, 1997 - v2
Checksum:i22BE64B963CB9739
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti965 – 966EQ → NS in K02498 (PubMed:6094528).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75128.1.
AP009048 Genomic DNA. Translation: BAA15920.1.
K02498 Genomic DNA. No translation available.
PIRiB64973.
RefSeqiNP_416571.1. NC_000913.3.
WP_000043761.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75128; AAC75128; b2067.
BAA15920; BAA15920; BAA15920.
GeneIDi946600.
KEGGiecj:JW2052.
eco:b2067.
PATRICi32119467. VBIEscCol129921_2144.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75128.1.
AP009048 Genomic DNA. Translation: BAA15920.1.
K02498 Genomic DNA. No translation available.
PIRiB64973.
RefSeqiNP_416571.1. NC_000913.3.
WP_000043761.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP38097.
SMRiP38097.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259682. 183 interactors.
IntActiP38097. 1 interactor.
STRINGi511145.b2067.

Proteomic databases

PaxDbiP38097.
PRIDEiP38097.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75128; AAC75128; b2067.
BAA15920; BAA15920; BAA15920.
GeneIDi946600.
KEGGiecj:JW2052.
eco:b2067.
PATRICi32119467. VBIEscCol129921_2144.

Organism-specific databases

EchoBASEiEB2297.
EcoGeneiEG12396. yegE.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG2202. LUCA.
COG3447. LUCA.
HOGENOMiHOG000122009.
InParanoidiP38097.
OMAiIRIDERF.
PhylomeDBiP38097.

Enzyme and pathway databases

UniPathwayiUPA00599.
BioCyciEcoCyc:EG12396-MONOMER.
ECOL316407:JW2052-MONOMER.

Miscellaneous databases

PROiP38097.

Family and domain databases

CDDicd01949. GGDEF. 1 hit.
Gene3Di3.20.20.450. 1 hit.
InterProiIPR001633. EAL_dom.
IPR000160. GGDEF_dom.
IPR007895. MASE1.
IPR029787. Nucleotide_cyclase.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013656. PAS_4.
IPR013655. PAS_fold_3.
[Graphical view]
PfamiPF00563. EAL. 1 hit.
PF00990. GGDEF. 1 hit.
PF05231. MASE1. 1 hit.
PF08447. PAS_3. 1 hit.
PF08448. PAS_4. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
SMARTiSM00052. EAL. 1 hit.
SM00267. GGDEF. 1 hit.
SM00086. PAC. 3 hits.
SM00091. PAS. 3 hits.
[Graphical view]
SUPFAMiSSF141868. SSF141868. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF55785. SSF55785. 3 hits.
TIGRFAMsiTIGR00254. GGDEF. 1 hit.
TIGR00229. sensory_box. 2 hits.
PROSITEiPS50883. EAL. 1 hit.
PS50887. GGDEF. 1 hit.
PS50113. PAC. 3 hits.
PS50112. PAS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYEGE_ECOLI
AccessioniPrimary (citable) accession number: P38097
Secondary accession number(s): P76391
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.