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P38095 (LAMA_EMENI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative urea carboxylase

EC=6.3.4.6
Alternative name(s):
Lactam utilization protein lamA
Urea amidolyase
Gene names
Name:lamA
ORF Names:AN0887
OrganismEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]
Taxonomic identifier227321 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length1241 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the utilization of lactams. Required for the conversion of exogenous 2-pyrrolidinone (gamma-butyrolactam) to endogenous gamma-amino-n-butyrate (GABA). Ref.4 Ref.5

Catalytic activity

ATP + urea + HCO3- = ADP + phosphate + urea-1-carboxylate.

Cofactor

Biotin By similarity.

Induction

By beta-alanine. Ref.5

Sequence similarities

Contains 1 ATP-grasp domain.

Contains 1 biotin carboxylation domain.

Contains 1 biotinyl-binding domain.

Sequence caution

The sequence AAA33312.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12411241Putative urea carboxylase
PRO_0000084354

Regions

Domain3 – 459457Biotin carboxylation
Domain121 – 321201ATP-grasp
Domain1159 – 123880Biotinyl-binding

Sites

Binding site1171ATP By similarity
Binding site2011ATP By similarity

Amino acid modifications

Modified residue12021N6-biotinyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P38095 [UniParc].

Last modified May 26, 2009. Version 2.
Checksum: 0534958F6957FA0F

FASTA1,241135,753
        10         20         30         40         50         60 
MEALKTLLIA NRGEIAVRVL KTAKKLNIRT IAVYTEPDAA STHVHLADEA ILLSGPPSKA 

        70         80         90        100        110        120 
YIDGDQIIDI AKRKGADAII PGYGFLSENS NFARDVASAG LAFVGPSPES IEAFGLKHTA 

       130        140        150        160        170        180 
RELATKAGVP IVPGSQGLVT SEDEAVKIAQ SLGFPVMLKA TAGGGGMGLL TCNTEKEVRE 

       190        200        210        220        230        240 
SFQTVQSRGE ALFKNAGLFI ERYYPSSHHI EVQVFGNGQG KAISIGEREC SIQRRHQKVI 

       250        260        270        280        290        300 
EECPSPFVTR NPELRKGLCD AAVRLAESID YGSAGTIEYL VDDESGKFFF LEMNTRLQVE 

       310        320        330        340        350        360 
HGITELCYGV DLVELMLRQA DAQLSGRKGL EAEFLSSIPV GAPQGFAIEA RVYAENPVRD 

       370        380        390        400        410        420 
FAPCPGILQD VDWKETTGSR IDTWVYRGIK VSANYDPLLA KVMYHASSRQ KAIEGLRDIL 

       430        440        450        460        470        480 
TGSRICGPPT NLGFLAEILA NKDFNAGNTL TKFLNNFEYN LAAIDVISGG AYTLIQDWPG 

       490        500        510        520        530        540 
RPTVGRGFCH SGPMDSVAFR IANALVGNPV GLEGLEITLS GPELRFLGPA VISLCGAPID 

       550        560        570        580        590        600 
AKLDEAPVPM WSRVKVSAGQ RLKIGKTTGG GCRAYLAVLG GFPNIAEWFG SKATAPMVGV 

       610        620        630        640        650        660 
GGYQGRQLTS GDYLTISAQI PESDNELSLP EHLIPQYPDS WELMSMPGPY DEGYLAPESI 

       670        680        690        700        710        720 
DMLYNAEWTI SHNAARGGIR LLGPKPTWAR PDGGEGGAHP SNLIECGYAI GSINWTGDDP 

       730        740        750        760        770        780 
VIFPQDAPDL GGFVSSHTIV KADLWKLGQV KAGDKLKFRA TSLKDTLLAR NELERFISDI 

       790        800        810        820        830        840 
VQCCQKGEDF GSITPLASSL PPAMSSSTRV SGIVHQIPEK GNQPLVSYRQ AGDDYLLIDY 

       850        860        870        880        890        900 
GVGAFDLNHR YRVTALKKVL SEAAGDISVS NGLINLVGCG NYLPKALMIY YDGTKIPQQK 

       910        920        930        940        950        960 
LIDYLCTIET QLGDLSRAKV PSRRFKLPLT FESKRQTDAI KRYMETQRPY ASYLPDNIDF 

       970        980        990       1000       1010       1020 
VARNNAFTRA ELENIYLTAS FMVITVGFFT ALPIALPVDP RQRMNCPKMN PSRVFTPAGQ 

      1030       1040       1050       1060       1070       1080 
VSWGGSCLAI YTVDSPGGYQ MNGMTIPGVD ILGTKRGYAP EKPWLFEDFD QITFYKVTEE 

      1090       1100       1110       1120       1130       1140 
EYERQLALFQ SGRYEYEWEV VEFDMAEHNR LLKETKEEVK AIRARQRKAQ AEMDLLEKEL 

      1150       1160       1170       1180       1190       1200 
LERWAKEKAE RGVSMDTVEE LLKDPEITVI EAPLNANVWK VEVKEGDKLD KDQVVVILEA 

      1210       1220       1230       1240 
MKLEIAVRAE SAAAGAVVEK ILAQPGKSIE AGKPLMLVRR G 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae."
Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. expand/collapse author list , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.
Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[2]"The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. expand/collapse author list , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[3]"Molecular characterization of the lam locus and sequences involved in regulation by the AmdR protein of Aspergillus nidulans."
Richardson I.B., Katz M.E., Hynes M.J.
Mol. Cell. Biol. 12:337-346(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-103.
Strain: biA1 niiA4.
[4]"Gene function identified by interspecific transformation."
Katz M.E., Hynes M.J.
Gene 78:167-171(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Characterization of the amdR-controlled lamA and lamB genes of Aspergillus nidulans."
Katz M.E., Hynes M.J.
Genetics 122:331-339(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AACD01000014 Genomic DNA. Translation: EAA65916.1.
M77283 Genomic DNA. Translation: AAA33312.1. Sequence problems.
BN001308 Genomic DNA. Translation: CBF88588.1.
PIRA42064.
RefSeqXP_658491.1. XM_653399.1.

3D structure databases

ProteinModelPortalP38095.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING162425.CADANIAP00001770.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADANIAT00001770; CADANIAP00001770; CADANIAG00001770.
GeneID2876661.
KEGGani:AN0887.2.

Phylogenomic databases

eggNOGCOG1984.
HOGENOMHOG000251581.
OMAPSNVHDC.
OrthoDBEOG7PGDZZ.

Family and domain databases

Gene3D2.40.100.10. 2 hits.
3.30.1360.40. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
InterProIPR003833. Allophan_hydro_1.
IPR003778. Allophan_hydro_2.
IPR024946. Arg_repress_C-like.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR029000. Cyclophilin-like_dom.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamPF02682. AHS1. 1 hit.
PF02626. AHS2. 1 hit.
PF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTSM00796. AHS1. 1 hit.
SM00797. AHS2. 1 hit.
SM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMSSF50891. SSF50891. 2 hits.
SSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLAMA_EMENI
AccessionPrimary (citable) accession number: P38095
Secondary accession number(s): C8VUT6, Q5BEZ3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: May 26, 2009
Last modified: June 11, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families