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P38095

- LAMA_EMENI

UniProt

P38095 - LAMA_EMENI

Protein

Putative urea carboxylase

Gene

lamA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 2 (26 May 2009)
      Previous versions | rss
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    Functioni

    Involved in the utilization of lactams. Required for the conversion of exogenous 2-pyrrolidinone (gamma-butyrolactam) to endogenous gamma-amino-n-butyrate (GABA).2 Publications

    Catalytic activityi

    ATP + urea + HCO3- = ADP + phosphate + urea-1-carboxylate.

    Cofactori

    Biotin.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei117 – 1171ATPBy similarity
    Binding sitei201 – 2011ATPBy similarity

    GO - Molecular functioni

    1. acetyl-CoA carboxylase activity Source: ASPGD
    2. ATP binding Source: UniProtKB-KW
    3. biotin carboxylase activity Source: InterPro
    4. hydrolase activity Source: UniProtKB-KW
    5. metal ion binding Source: InterPro
    6. methylcrotonoyl-CoA carboxylase activity Source: ASPGD
    7. urea carboxylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbon utilization Source: ASPGD
    2. fatty acid biosynthetic process Source: ASPGD
    3. leucine catabolic process Source: ASPGD
    4. nitrogen compound metabolic process Source: ASPGD

    Keywords - Molecular functioni

    Hydrolase, Ligase

    Keywords - Ligandi

    ATP-binding, Biotin, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative urea carboxylase (EC:6.3.4.6)
    Alternative name(s):
    Lactam utilization protein lamA
    Urea amidolyase
    Gene namesi
    Name:lamA
    ORF Names:AN0887
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000000560: Chromosome VIII

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: ASPGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12411241Putative urea carboxylasePRO_0000084354Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1202 – 12021N6-biotinyllysineBy similarity

    Expressioni

    Inductioni

    By beta-alanine.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi162425.CADANIAP00001770.

    Structurei

    3D structure databases

    ProteinModelPortaliP38095.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 459457Biotin carboxylationAdd
    BLAST
    Domaini121 – 321201ATP-graspPROSITE-ProRule annotationAdd
    BLAST
    Domaini1159 – 123880Biotinyl-bindingAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ATP-grasp domain.PROSITE-ProRule annotation
    Contains 1 biotin carboxylation domain.Curated
    Contains 1 biotinyl-binding domain.Curated

    Phylogenomic databases

    eggNOGiCOG1984.
    HOGENOMiHOG000251581.
    OMAiPSNVHDC.
    OrthoDBiEOG7PGDZZ.

    Family and domain databases

    Gene3Di2.40.100.10. 2 hits.
    3.30.1360.40. 1 hit.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    InterProiIPR003833. Allophan_hydro_1.
    IPR003778. Allophan_hydro_2.
    IPR024946. Arg_repress_C-like.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR001882. Biotin_BS.
    IPR011764. Biotin_carboxylation_dom.
    IPR005482. Biotin_COase_C.
    IPR000089. Biotin_lipoyl.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR029000. Cyclophilin-like_dom.
    IPR016185. PreATP-grasp_dom.
    IPR011054. Rudment_hybrid_motif.
    IPR011053. Single_hybrid_motif.
    [Graphical view]
    PfamiPF02682. AHS1. 1 hit.
    PF02626. AHS2. 1 hit.
    PF02785. Biotin_carb_C. 1 hit.
    PF00364. Biotin_lipoyl. 1 hit.
    PF00289. CPSase_L_chain. 1 hit.
    PF02786. CPSase_L_D2. 1 hit.
    [Graphical view]
    SMARTiSM00796. AHS1. 1 hit.
    SM00797. AHS2. 1 hit.
    SM00878. Biotin_carb_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF50891. SSF50891. 2 hits.
    SSF51230. SSF51230. 1 hit.
    SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS50979. BC. 1 hit.
    PS00188. BIOTIN. 1 hit.
    PS50968. BIOTINYL_LIPOYL. 1 hit.
    PS00866. CPSASE_1. 1 hit.
    PS00867. CPSASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38095-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEALKTLLIA NRGEIAVRVL KTAKKLNIRT IAVYTEPDAA STHVHLADEA     50
    ILLSGPPSKA YIDGDQIIDI AKRKGADAII PGYGFLSENS NFARDVASAG 100
    LAFVGPSPES IEAFGLKHTA RELATKAGVP IVPGSQGLVT SEDEAVKIAQ 150
    SLGFPVMLKA TAGGGGMGLL TCNTEKEVRE SFQTVQSRGE ALFKNAGLFI 200
    ERYYPSSHHI EVQVFGNGQG KAISIGEREC SIQRRHQKVI EECPSPFVTR 250
    NPELRKGLCD AAVRLAESID YGSAGTIEYL VDDESGKFFF LEMNTRLQVE 300
    HGITELCYGV DLVELMLRQA DAQLSGRKGL EAEFLSSIPV GAPQGFAIEA 350
    RVYAENPVRD FAPCPGILQD VDWKETTGSR IDTWVYRGIK VSANYDPLLA 400
    KVMYHASSRQ KAIEGLRDIL TGSRICGPPT NLGFLAEILA NKDFNAGNTL 450
    TKFLNNFEYN LAAIDVISGG AYTLIQDWPG RPTVGRGFCH SGPMDSVAFR 500
    IANALVGNPV GLEGLEITLS GPELRFLGPA VISLCGAPID AKLDEAPVPM 550
    WSRVKVSAGQ RLKIGKTTGG GCRAYLAVLG GFPNIAEWFG SKATAPMVGV 600
    GGYQGRQLTS GDYLTISAQI PESDNELSLP EHLIPQYPDS WELMSMPGPY 650
    DEGYLAPESI DMLYNAEWTI SHNAARGGIR LLGPKPTWAR PDGGEGGAHP 700
    SNLIECGYAI GSINWTGDDP VIFPQDAPDL GGFVSSHTIV KADLWKLGQV 750
    KAGDKLKFRA TSLKDTLLAR NELERFISDI VQCCQKGEDF GSITPLASSL 800
    PPAMSSSTRV SGIVHQIPEK GNQPLVSYRQ AGDDYLLIDY GVGAFDLNHR 850
    YRVTALKKVL SEAAGDISVS NGLINLVGCG NYLPKALMIY YDGTKIPQQK 900
    LIDYLCTIET QLGDLSRAKV PSRRFKLPLT FESKRQTDAI KRYMETQRPY 950
    ASYLPDNIDF VARNNAFTRA ELENIYLTAS FMVITVGFFT ALPIALPVDP 1000
    RQRMNCPKMN PSRVFTPAGQ VSWGGSCLAI YTVDSPGGYQ MNGMTIPGVD 1050
    ILGTKRGYAP EKPWLFEDFD QITFYKVTEE EYERQLALFQ SGRYEYEWEV 1100
    VEFDMAEHNR LLKETKEEVK AIRARQRKAQ AEMDLLEKEL LERWAKEKAE 1150
    RGVSMDTVEE LLKDPEITVI EAPLNANVWK VEVKEGDKLD KDQVVVILEA 1200
    MKLEIAVRAE SAAAGAVVEK ILAQPGKSIE AGKPLMLVRR G 1241
    Length:1,241
    Mass (Da):135,753
    Last modified:May 26, 2009 - v2
    Checksum:i0534958F6957FA0F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACD01000014 Genomic DNA. Translation: EAA65916.1.
    M77283 Genomic DNA. Translation: AAA33312.1. Sequence problems.
    BN001308 Genomic DNA. Translation: CBF88588.1.
    PIRiA42064.
    RefSeqiXP_658491.1. XM_653399.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00001770; CADANIAP00001770; CADANIAG00001770.
    GeneIDi2876661.
    KEGGiani:AN0887.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACD01000014 Genomic DNA. Translation: EAA65916.1 .
    M77283 Genomic DNA. Translation: AAA33312.1 . Sequence problems.
    BN001308 Genomic DNA. Translation: CBF88588.1 .
    PIRi A42064.
    RefSeqi XP_658491.1. XM_653399.1.

    3D structure databases

    ProteinModelPortali P38095.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 162425.CADANIAP00001770.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADANIAT00001770 ; CADANIAP00001770 ; CADANIAG00001770 .
    GeneIDi 2876661.
    KEGGi ani:AN0887.2.

    Phylogenomic databases

    eggNOGi COG1984.
    HOGENOMi HOG000251581.
    OMAi PSNVHDC.
    OrthoDBi EOG7PGDZZ.

    Family and domain databases

    Gene3Di 2.40.100.10. 2 hits.
    3.30.1360.40. 1 hit.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    InterProi IPR003833. Allophan_hydro_1.
    IPR003778. Allophan_hydro_2.
    IPR024946. Arg_repress_C-like.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR001882. Biotin_BS.
    IPR011764. Biotin_carboxylation_dom.
    IPR005482. Biotin_COase_C.
    IPR000089. Biotin_lipoyl.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR029000. Cyclophilin-like_dom.
    IPR016185. PreATP-grasp_dom.
    IPR011054. Rudment_hybrid_motif.
    IPR011053. Single_hybrid_motif.
    [Graphical view ]
    Pfami PF02682. AHS1. 1 hit.
    PF02626. AHS2. 1 hit.
    PF02785. Biotin_carb_C. 1 hit.
    PF00364. Biotin_lipoyl. 1 hit.
    PF00289. CPSase_L_chain. 1 hit.
    PF02786. CPSase_L_D2. 1 hit.
    [Graphical view ]
    SMARTi SM00796. AHS1. 1 hit.
    SM00797. AHS2. 1 hit.
    SM00878. Biotin_carb_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50891. SSF50891. 2 hits.
    SSF51230. SSF51230. 1 hit.
    SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS50979. BC. 1 hit.
    PS00188. BIOTIN. 1 hit.
    PS50968. BIOTINYL_LIPOYL. 1 hit.
    PS00866. CPSASE_1. 1 hit.
    PS00867. CPSASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    2. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
      Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
      , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
      Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    3. "Molecular characterization of the lam locus and sequences involved in regulation by the AmdR protein of Aspergillus nidulans."
      Richardson I.B., Katz M.E., Hynes M.J.
      Mol. Cell. Biol. 12:337-346(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-103.
      Strain: biA1 niiA4.
    4. "Gene function identified by interspecific transformation."
      Katz M.E., Hynes M.J.
      Gene 78:167-171(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Characterization of the amdR-controlled lamA and lamB genes of Aspergillus nidulans."
      Katz M.E., Hynes M.J.
      Genetics 122:331-339(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiLAMA_EMENI
    AccessioniPrimary (citable) accession number: P38095
    Secondary accession number(s): C8VUT6, Q5BEZ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: May 26, 2009
    Last modified: October 1, 2014
    This is version 70 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3