P38089 (PP2C4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C homolog 4
Short name=PP2C-4
EC=3.1.3.16

Gene names

Name:	PTC4
Synonyms:	GCT1
Ordered Locus Names:	YBR125C
ORF Names:	YBR0921

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Miscellaneous	Present with 149 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from direct assay PubMed 10580002. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay PubMed 10580002. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PAA1	Q12447	6	EBI-12814,EBI-33397

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Protein phosphatase 2C homolog 4		PRO_0000057777
Sites
	Metal binding	83	1	Manganese 1 By similarity
	Metal binding	83	1	Manganese 2 By similarity
	Metal binding	84	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	310	1	Manganese 2 By similarity
	Metal binding	359	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38089 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 27F2903048541B6A	FASTA	393	44,176
	10 20 30 40 50 60 MGQLLSHPLT EKTIEYNEYK NNQASTGIVP RFYNCVGSMQ GYRLTQEDAH LIRNENSVVY 70 80 90 100 110 120 VRFFNPFIDK YETLSLNVFA VFDGHGGDDC SKFLSGGRHH RDGNGSSNGN GEPNAGLIKW 130 140 150 160 170 180 IAYSFENHHY TSTTNNDSSK FKRSFNTLEG LVSQIFKDAF ILQDEELYRH FANSSCGSTA 190 200 210 220 230 240 VVACIINEES LYVANCGDSR CILSSKSNGI KTMSFDHKPQ HIGELIRIND NGGTVSLGRV 250 260 270 280 290 300 GGVLALSRAF SDFQFKRGVT YPHRRTKLTN ITQNLTYGTP PQEAQVTVEP DVLMHKIDYS 310 320 330 340 350 360 KDEFLVLACD GIWDIYNNKQ LIHFIKYHLV SGTKLDTIIT KLLDHGIAQA NSNTGVGFDN 370 380 390 MTAIIVVLNR KGETLQDWFN KMKTRLERER GLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78993 Genomic DNA. Translation: CAA55626.1. Z35994 Genomic DNA. Translation: CAA85082.1. BK006936 Genomic DNA. Translation: DAA07242.1.
PIR	S48288.
RefSeq	NP_009683.1. NM_001178473.1.
3D structure databases
ProteinModelPortal	P38089.
SMR	P38089. Positions 1-369.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6437N.
IntAct	P38089. 10 interactions.
MINT	MINT-397378.
STRING	4932.YBR125C.
Proteomic databases
PaxDb	P38089.
PeptideAtlas	P38089.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR125C; YBR125C; YBR125C.
GeneID	852422.
KEGG	sce:YBR125C.
Organism-specific databases
CYGD	YBR125c.
SGD	S000000329. PTC4.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
KO	K01090.
OMA	PRFYNCV.
OrthoDB	EOG405W8X.
Enzyme and pathway databases
BioCyc	YEAST:G3O-29081-MONOMER.
Gene expression databases
Genevestigator	P38089.
GermOnline	YBR125C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971288.

Entry information

Entry name

PP2C4_YEAST

Accession

Primary (citable) accession number: P38089
Secondary accession number(s): D6VQC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 29, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program