Leu/Val/Ile amino-acid permease - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38084 (BAP2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 106. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Leu/Val/Ile amino-acid permease

Alternative name(s):
Branched-chain amino-acid permease 2

Gene names

Name:	BAP2
Ordered Locus Names:	YBR068C
ORF Names:	YBR0629

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Permease for leucine, valine and isoleucine. Also transports cysteine, methionine, phenyalanine, tyrosine and tryptophan. Ref.4 Ref.5
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.6.
Miscellaneous	Present with 8660 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family. [View classification]

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino acid transmembrane transporter activity Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 609

609

Leu/Val/Ile amino-acid permease

PRO_0000054146

Regions

Topological domain

1 – 99

Cytoplasmic Potential

Transmembrane

100 – 120

Helical; Potential

Topological domain

121 – 123

Extracellular Potential

Transmembrane

124 – 144

Helical; Potential

Topological domain

145 – 173

Cytoplasmic Potential

Transmembrane

174 – 194

Helical; Potential

Topological domain

195 – 210

Extracellular Potential

Transmembrane

211 – 231

Helical; Potential

Topological domain

232 – 235

Cytoplasmic Potential

Transmembrane

236 – 256

Helical; Potential

Topological domain

257 – 289

Extracellular Potential

Transmembrane

290 – 310

Helical; Potential

Topological domain

311 – 328

Cytoplasmic Potential

Transmembrane

329 – 349

Helical; Potential

Topological domain

350 – 374

Extracellular Potential

Transmembrane

375 – 395

Helical; Potential

Topological domain

396 – 427

Cytoplasmic Potential

Transmembrane

428 – 448

Helical; Potential

Topological domain

449 – 451

Extracellular Potential

Transmembrane

452 – 472

Helical; Potential

Topological domain

473 – 500

Cytoplasmic Potential

Transmembrane

501 – 521

Helical; Potential

Topological domain

522 – 536

Extracellular Potential

Transmembrane

537 – 557

Helical; Potential

Topological domain

558 – 609

Cytoplasmic Potential

Amino acid modifications

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphothreonine Ref.9 Ref.11

Modified residue

Phosphoserine Ref.10 Ref.11

Modified residue

Phosphoserine Ref.10 Ref.11

Modified residue

Phosphoserine Ref.11

Modified residue

Phosphoserine Ref.9 Ref.11

Modified residue

Phosphoserine Ref.11

Experimental info

Sequence conflict

139

V → E in CAA85012. Ref.1

Sequence conflict

203

W → G in CAA85012. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P38084 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: B87FD6A7464E07F5
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FASTA

609

67,769

        10         20         30         40         50         60 
MLSSEDFGSS GKKETSPDSI SIRSFSAGNN FQSSSSEKTY SKQKSGSDKL IHRFADSFKR 

        70         80         90        100        110        120 
AEGSTTRTKQ INENTSDLED GVESITSDSK LKKSMKSRHV VMMSLGTGIG TGLLVANAKG 

       130        140        150        160        170        180 
LHYGGPAALI IGYILVSFVT YFMIQAAGEM AVTYPTLPAN FNAYSSIFIS KSFGFATVWL 

       190        200        210        220        230        240 
YCFQWLTVLP LELITASMTI QFWNDKINPD IYILIFYVFL VFIHFFGVKA YGETEFIFNC 

       250        260        270        280        290        300 
CKILMIAGFI ILSIVINCGG AGNDGYIGAT YWHNPGAFAG DTSIGRFKNV CYILVTAYFS 

       310        320        330        340        350        360 
FGGMELFALS VQEQSNPRKS TPVAAKRSIY RIVVIYLLTM ILIGFNVPYN DDQLMGAGGS 

       370        380        390        400        410        420 
ATHASPYVLA ASIHGVKIVP HIINAVILIS VVSVANSSLY AGPRLICSLA QQGYAPKFLD 

       430        440        450        460        470        480 
YVDREGRPLR ALIVCCVFGV IAFVAASSKE EIVFTWLAAI AGLSELFTWT SIMLSHLRFR 

       490        500        510        520        530        540 
QAMKVQGRSL DELGYKATTG IWGSIYGVFF NILVFVAQFW VALAPLGNGG KCDAESFFQN 

       550        560        570        580        590        600 
YLAFPIWLAF YFGYMVYNRD FTLLNPLDKI DLDFHRRIYD PELMRQEDEE NKEKLRNMSL 


MRKAYHFWC

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 139 AND 203. Strain: ATCC 204508 / S288c.
[3]	"BAP2, a gene encoding a permease for branched-chain amino acids in Saccharomyces cerevisiae." Grauslund M., Didion T., Kielland-Brandt M.C., Andersen H.A. Biochim. Biophys. Acta 1269:275-280(1995) [PubMed: 7495881] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Cysteine uptake by Saccharomyces cerevisiae is accomplished by multiple permeases." During-Olsen L., Regenberg B., Gjermansen C., Kielland-Brandt M.C., Hansen J. Curr. Genet. 35:609-617(1999) [PubMed: 10467005] [Abstract] Cited for: FUNCTION IN L-CYSTEINE UPTAKE.
[5]	"Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae." Regenberg B., During-Olsen L., Kielland-Brandt M.C., Holmberg S. Curr. Genet. 36:317-328(1999) [PubMed: 10654085] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-15 AND SER-24, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16 AND SER-19, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3; SER-4; THR-15; SER-16; SER-19; SER-21; SER-24 AND SER-76, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z35937 Genomic DNA. Translation: CAA85012.1. BK006936 Genomic DNA. Translation: DAA07187.2.
PIR	S45930.
RefSeq	NP_009624.2. NM_001178416.2.
3D structure databases
ProteinModelPortal	P38084.
SMR	P38084. Positions 97-152.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4910N.
IntAct	P38084. 19 interactions.
MINT	MINT-516615.
STRING	P38084.
Protein family/group databases
TCDB	2.A.3.10.6. amino acid-polyamine-organocation (APC) family.
Proteomic databases
PeptideAtlas	P38084.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	852360.
KEGG	sce:YBR068C.
NMPDR	fig\|4932.3.peg.321.
Organism-specific databases
CYGD	YBR068c.
SGD	S000000272. BAP2.
Phylogenomic databases
eggNOG	fuNOG05379.
GeneTree	EFGT00050000001131.
HOGENOM	HBG492579.
OMA	DFHRRIY.
OrthoDB	EOG48D43Q.
Gene expression databases
ArrayExpress	P38084.
Genevestigator	P38084.
GermOnline	YBR068C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004841. AA-permease_dom. IPR002293. AA/rel_permease1. IPR004762. Amino_acid_permease_fungi. IPR004840. Amoino_acid_permease_CS. [Graphical view]
PANTHER	PTHR11785. AA/rel_permease1. 1 hit.
Pfam	PF00324. AA_permease. 1 hit. [Graphical view]
PIRSF	PIRSF006060. AA_transporter. 1 hit.
TIGRFAMs	TIGR00913. 2A0310. 1 hit.
PROSITE	PS00218. AMINO_ACID_PERMEASE_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971125.

Entry information

Entry name

BAP2_YEAST

Accession

Primary (citable) accession number: P38084
Secondary accession number(s): D6VQ67

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	July 27, 2011
Last modified:	December 14, 2011
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents