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P38080

- AKL1_YEAST

UniProt

P38080 - AKL1_YEAST

Protein

Serine/threonine-protein kinase AKL1

Gene

AKL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei70 – 701ATPPROSITE-ProRule annotation
    Active sitei181 – 1811Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi41 – 499ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. identical protein binding Source: IntAct
    3. protein kinase activity Source: SGD
    4. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. actin cytoskeleton organization Source: SGD
    2. regulation of endocytosis Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29030-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase AKL1 (EC:2.7.11.1)
    Gene namesi
    Name:AKL1
    Ordered Locus Names:YBR059C
    ORF Names:YBR0519
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR059c.
    SGDiS000000263. AKL1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 11081107Serine/threonine-protein kinase AKL1PRO_0000085603Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei10 – 101Phosphoserine1 Publication
    Modified residuei407 – 4071Phosphoserine2 Publications
    Modified residuei471 – 4711Phosphothreonine1 Publication
    Modified residuei504 – 5041Phosphoserine2 Publications
    Modified residuei541 – 5411Phosphoserine3 Publications
    Modified residuei574 – 5741Phosphoserine1 Publication
    Modified residuei801 – 8011Phosphoserine2 Publications
    Modified residuei846 – 8461Phosphoserine2 Publications
    Modified residuei953 – 9531Phosphoserine1 Publication
    Modified residuei960 – 9601Phosphoserine1 Publication
    Cross-linki1008 – 1008Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Cross-linki1046 – 1046Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
    Modified residuei1048 – 10481Phosphoserine1 Publication
    Modified residuei1072 – 10721Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiP38080.
    PaxDbiP38080.
    PeptideAtlasiP38080.

    Expressioni

    Gene expression databases

    GenevestigatoriP38080.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-9528,EBI-9528

    Protein-protein interaction databases

    BioGridi32763. 143 interactions.
    DIPiDIP-1717N.
    IntActiP38080. 26 interactions.
    MINTiMINT-409952.
    STRINGi4932.YBR059C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38080.
    SMRiP38080. Positions 25-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 319285Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00510000046552.
    KOiK08286.
    OMAiQNIYVHT.
    OrthoDBiEOG74BK1F.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38080-1 [UniParc]FASTAAdd to Basket

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    MSITNGTSRS VSAMGHPAVE RYTPGHIVCV GTHKVEVVNY LAEGGFAQIY     50
    VVKFLEYLNE FDNTASVPLK IGDVACLKRV LVQDENGLNE MRNEVEVMKK 100
    LKGAPNIVQY FDSNASRRRD GVQGFEVLLL MELCPNKSLL DYMNQRLSTK 150
    LTEAEIVKIM YDVALSISQM HYLPVSLIHR DIKIENVLVD AKNNFKLADF 200
    GSTSTCFPIV TTHQDIALLT QNIYVHTTPQ YRSPEMIDLY RCLPINEKSD 250
    IWALGVFLYK LLFFTTPFEM TGQFAILHSK YEFPVNKYSS KLINLIIIML 300
    AENPNLRPNI YQVLYHLCEI LNVEVPIEDK YAEGAYNFSK YTQFQNKLQN 350
    VQLQMYQLQQ KKIMQNNKLS DSEENLLNDM FLSSFEISSK LPMNASDGHA 400
    AVSRIPSQNV GQELEEEKES QSDQRKSTLS EDKSSRTTSN ANSSGTANNP 450
    QEINTIQSPG IEDKSIFENK TPGELYYPSV SELDTYLDKE LVKQSSDPTI 500
    SEQSPRLNTQ SLPQRQKSTS SYSSGGRSMK STSYGAATIG SDEALANEKT 550
    AGINKMKQHK SNNPFPKMNV AYHSTNELSN DASNFFLEEQ QQGQRYQQAQ 600
    NQTGTQGNTF PDESQYQSRV EQQQQQQDQP KGPANYSQRN FYTGRDRSNK 650
    PMQLGGTIAG DSGNRRVNFQ NISQNYATNS QSGYLPSQNS PAIPMVRPVI 700
    SMNQQQAQQI QAQQLQAQQM QAKQQMQAKQ QMQVQQQLQV QQQMQIQNAN 750
    NNGTYVSDRT NHTTEDMRNA QGGEPPILAG NSANEPMHSS SKNEALLIEL 800
    SPLKEDAGKQ SFQDTNEPQT GGIEDAGGSG TIKGSNNNRN GVLNLSLNEM 850
    DLSRDDTGAA VSSFSSSSSS ASIQQAKLSG RKGSSKRNNY STDELGDSMV 900
    SSESIDIDLD DARRGKTAER RPLHNERGHK DQARSSDASK SNQFKSKDFS 950
    SVSTRQPRQS LDLNFQEVNL SSPTLTQEHR NKNDSPAPNS HHSYRVSPHA 1000
    STAITENKRH STGHELSTRS NGKHETHRTG SKQRHDLERY RHSKDKDSNS 1050
    SITISTSTPS EMRKSFARAR QSLDLERVRR EAMASSASSS GGSNGKRRSF 1100
    FSVFRSEK 1108
    Length:1,108
    Mass (Da):123,989
    Last modified:October 1, 1994 - v1
    Checksum:iAEC6CB0A707164E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z35928 Genomic DNA. Translation: CAA85002.1.
    BK006936 Genomic DNA. Translation: DAA07179.1.
    PIRiS45917.
    RefSeqiNP_009615.1. NM_001178407.1.

    Genome annotation databases

    EnsemblFungiiYBR059C; YBR059C; YBR059C.
    GeneIDi852351.
    KEGGisce:YBR059C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z35928 Genomic DNA. Translation: CAA85002.1 .
    BK006936 Genomic DNA. Translation: DAA07179.1 .
    PIRi S45917.
    RefSeqi NP_009615.1. NM_001178407.1.

    3D structure databases

    ProteinModelPortali P38080.
    SMRi P38080. Positions 25-365.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32763. 143 interactions.
    DIPi DIP-1717N.
    IntActi P38080. 26 interactions.
    MINTi MINT-409952.
    STRINGi 4932.YBR059C.

    Proteomic databases

    MaxQBi P38080.
    PaxDbi P38080.
    PeptideAtlasi P38080.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR059C ; YBR059C ; YBR059C .
    GeneIDi 852351.
    KEGGi sce:YBR059C.

    Organism-specific databases

    CYGDi YBR059c.
    SGDi S000000263. AKL1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00510000046552.
    KOi K08286.
    OMAi QNIYVHT.
    OrthoDBi EOG74BK1F.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29030-MONOMER.

    Miscellaneous databases

    NextBioi 971101.

    Gene expression databases

    Genevestigatori P38080.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    4. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1008 AND LYS-1046.
      Strain: SUB592.
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-541; SER-801 AND SER-1072, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407; THR-471; SER-504; SER-541 AND SER-846, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; SER-407; SER-504; SER-541; SER-574; SER-801; SER-846; SER-953; SER-960 AND SER-1048, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAKL1_YEAST
    AccessioniPrimary (citable) accession number: P38080
    Secondary accession number(s): D6VQ59
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3000 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3