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P38077

- ATPG_YEAST

UniProt

P38077 - ATPG_YEAST

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Protein
ATP synthase subunit gamma, mitochondrial
Gene
ATP3, ATP3a, ATP3b, YBR039W, YBR0408
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F1 domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha3beta3. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

GO - Molecular functioni

  1. proton-transporting ATP synthase activity, rotational mechanism Source: InterPro
  2. proton-transporting ATPase activity, rotational mechanism Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. ATP synthesis coupled proton transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

ATP synthesis, Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29013-MONOMER.

Protein family/group databases

TCDBi3.A.2.1.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP synthase subunit gamma, mitochondrial
Alternative name(s):
F-ATPase gamma subunit
Gene namesi
Name:ATP3
Synonyms:ATP3a, ATP3b
Ordered Locus Names:YBR039W
ORF Names:YBR0408
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR039w.
SGDiS000000243. ATP3.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial proton-transporting ATP synthase, central stalk Source: SGD
  2. proton-transporting ATP synthase complex, catalytic core F(1) Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

CF(1), Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi273 – 2731A → V: Lower activity. 1 Publication
Mutagenesisi297 – 2971T → A in ATP3-5; dominant suppressor of the slow-growth phenotype of yme1 strains lacking mitochondrial DNA. 1 Publication
Mutagenesisi303 – 3031I → T in ATP3-1; dominant suppressor of the slow-growth phenotype of yme1 strains lacking mitochondrial DNA. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3333Mitochondrion1 Publication
Add
BLAST
Chaini34 – 311278ATP synthase subunit gamma, mitochondrial
PRO_0000002691Add
BLAST

Proteomic databases

MaxQBiP38077.
PaxDbiP38077.
PeptideAtlasiP38077.

Expressioni

Gene expression databases

GenevestigatoriP38077.

Interactioni

Subunit structurei

F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c.

Protein-protein interaction databases

BioGridi32740. 48 interactions.
DIPiDIP-3035N.
IntActiP38077. 23 interactions.
MINTiMINT-693544.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi36 – 7944
Helixi83 – 875
Beta strandi105 – 1106
Helixi120 – 13415
Beta strandi139 – 1446
Helixi145 – 15410
Helixi156 – 1583
Beta strandi159 – 1657
Beta strandi167 – 1693
Helixi173 – 18614
Helixi189 – 1913
Beta strandi193 – 2019
Beta strandi203 – 2064
Beta strandi209 – 2157
Helixi218 – 2225
Helixi227 – 2293
Beta strandi234 – 2363
Helixi239 – 30870

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HLDX-ray2.80G/P/Y34-311[»]
2WPDX-ray3.43G34-311[»]
2XOKX-ray3.01G1-311[»]
3FKSX-ray3.59G/P/Y34-311[»]
3OE7X-ray3.19G/P/Y34-311[»]
3OEEX-ray2.74G/P/Y34-311[»]
3OEHX-ray3.00G/P/Y34-311[»]
3OFNX-ray3.20G/P/Y34-311[»]
3ZIAX-ray2.50G/Q34-311[»]
3ZRYX-ray6.50G34-311[»]
4B2Qelectron microscopy37.00G/g34-311[»]
ProteinModelPortaliP38077.
SMRiP38077. Positions 34-311.

Miscellaneous databases

EvolutionaryTraceiP38077.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0224.
GeneTreeiENSGT00390000006837.
HOGENOMiHOG000215911.
KOiK02136.
OMAiMATLKDX.
OrthoDBiEOG76QFTN.

Family and domain databases

InterProiIPR000131. ATPase_F1-cplx_gsu.
IPR023632. ATPase_F1_gsu_CS.
IPR023633. ATPase_F1_gsu_dom.
[Graphical view]
PANTHERiPTHR11693. PTHR11693. 1 hit.
PfamiPF00231. ATP-synt. 1 hit.
[Graphical view]
PRINTSiPR00126. ATPASEGAMMA.
SUPFAMiSSF52943. SSF52943. 1 hit.
TIGRFAMsiTIGR01146. ATPsyn_F1gamma. 1 hit.
PROSITEiPS00153. ATPASE_GAMMA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38077-1 [UniParc]FASTAAdd to Basket

« Hide

MLSRIVSNNA TRSVMCHQAQ VGILYKTNPV RTYATLKEVE MRLKSIKNIE    50
KITKTMKIVA STRLSKAEKA KISAKKMDEA EQLFYKNAET KNLDVEATET 100
GAPKELIVAI TSDKGLCGSI HSQLAKAVRR HLNDQPNADI VTIGDKIKMQ 150
LLRTHPNNIK LSINGIGKDA PTFQESALIA DKLLSVMKAG TYPKISIFYN 200
DPVSSLSFEP SEKPIFNAKT IEQSPSFGKF EIDTDANVPR DLFEYTLANQ 250
MLTAMAQGYA AEISARRNAM DNASKNAGDM INRYSILYNR TRQAVITNEL 300
VDIITGASSL G 311
Length:311
Mass (Da):34,351
Last modified:October 1, 1994 - v1
Checksum:iADC71F3C1E0CDF91
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti207 – 2071S → I in strain: D273-10B/A1.
Natural varianti308 – 3081S → F in allele ATP3a.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09305 Genomic DNA. Translation: AAA62605.1.
U08318 Unassigned DNA. Translation: AAA88816.1.
AB036928 Genomic DNA. Translation: BAC97839.1.
AB036929 Genomic DNA. Translation: BAC97840.1.
Z35908 Genomic DNA. Translation: CAA84981.1.
AY557865 Genomic DNA. Translation: AAS56191.1.
BK006936 Genomic DNA. Translation: DAA07159.1.
PIRiS55891.
RefSeqiNP_009595.1. NM_001178387.1.

Genome annotation databases

EnsemblFungiiYBR039W; YBR039W; YBR039W.
GeneIDi852327.
KEGGisce:YBR039W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09305 Genomic DNA. Translation: AAA62605.1 .
U08318 Unassigned DNA. Translation: AAA88816.1 .
AB036928 Genomic DNA. Translation: BAC97839.1 .
AB036929 Genomic DNA. Translation: BAC97840.1 .
Z35908 Genomic DNA. Translation: CAA84981.1 .
AY557865 Genomic DNA. Translation: AAS56191.1 .
BK006936 Genomic DNA. Translation: DAA07159.1 .
PIRi S55891.
RefSeqi NP_009595.1. NM_001178387.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2HLD X-ray 2.80 G/P/Y 34-311 [» ]
2WPD X-ray 3.43 G 34-311 [» ]
2XOK X-ray 3.01 G 1-311 [» ]
3FKS X-ray 3.59 G/P/Y 34-311 [» ]
3OE7 X-ray 3.19 G/P/Y 34-311 [» ]
3OEE X-ray 2.74 G/P/Y 34-311 [» ]
3OEH X-ray 3.00 G/P/Y 34-311 [» ]
3OFN X-ray 3.20 G/P/Y 34-311 [» ]
3ZIA X-ray 2.50 G/Q 34-311 [» ]
3ZRY X-ray 6.50 G 34-311 [» ]
4B2Q electron microscopy 37.00 G/g 34-311 [» ]
ProteinModelPortali P38077.
SMRi P38077. Positions 34-311.
ModBasei Search...

Protein-protein interaction databases

BioGridi 32740. 48 interactions.
DIPi DIP-3035N.
IntActi P38077. 23 interactions.
MINTi MINT-693544.

Protein family/group databases

TCDBi 3.A.2.1.3. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Proteomic databases

MaxQBi P38077.
PaxDbi P38077.
PeptideAtlasi P38077.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR039W ; YBR039W ; YBR039W .
GeneIDi 852327.
KEGGi sce:YBR039W.

Organism-specific databases

CYGDi YBR039w.
SGDi S000000243. ATP3.

Phylogenomic databases

eggNOGi COG0224.
GeneTreei ENSGT00390000006837.
HOGENOMi HOG000215911.
KOi K02136.
OMAi MATLKDX.
OrthoDBi EOG76QFTN.

Enzyme and pathway databases

BioCyci YEAST:G3O-29013-MONOMER.

Miscellaneous databases

EvolutionaryTracei P38077.
NextBioi 971035.
PROi P38077.

Gene expression databases

Genevestigatori P38077.

Family and domain databases

InterProi IPR000131. ATPase_F1-cplx_gsu.
IPR023632. ATPase_F1_gsu_CS.
IPR023633. ATPase_F1_gsu_dom.
[Graphical view ]
PANTHERi PTHR11693. PTHR11693. 1 hit.
Pfami PF00231. ATP-synt. 1 hit.
[Graphical view ]
PRINTSi PR00126. ATPASEGAMMA.
SUPFAMi SSF52943. SSF52943. 1 hit.
TIGRFAMsi TIGR01146. ATPsyn_F1gamma. 1 hit.
PROSITEi PS00153. ATPASE_GAMMA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the yeast ATP3 gene coding for the gamma-subunit of F1 and characterization of atp3 mutants."
    Paul M.-F., Ackermann S., Yue J., Arselin G., Velours J., Tzagoloff A.
    J. Biol. Chem. 269:26158-26164(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 34-61; 77-86; 195-207; 220-240 AND 284-290, MUTAGENESIS OF ALA-273.
    Strain: ATCC 201238 / W303-1B and D273-10B/A1.
  2. "Mutations in the mitochondrial ATP synthase gamma subunit suppress a slow-growth phenotype of yme1 yeast lacking mitochondrial DNA."
    Weber E.R., Rooks R.S., Shafer K.S., Chase J.W., Thorsness P.E.
    Genetics 140:435-442(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF THR-297 AND ILE-303.
  3. "Studies on the ATP3 gene of Saccharomyces cerevisiae: presence of two closely linked copies, ATP3a and ATP3b, on the right arm of chromosome II."
    Ohnishi K., Ishibashi S., Kunihiro M., Satoh T., Matsubara K., Oku S., Ono B., Mabuchi T., Takeda M.
    Yeast 20:943-954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELES ATP3A AND ATP3B).
    Strain: ATCC 64665 / S288c / DC5.
  4. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Molecular architecture of the rotary motor in ATP synthase."
    Stock D., Leslie A.G., Walker J.E.
    Science 286:1700-1705(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING.
  9. "Novel features of the rotary catalytic mechanism revealed in the structure of yeast F1 ATPase."
    Kabaleeswaran V., Puri N., Walker J.E., Leslie A.G.W., Mueller D.M.
    EMBO J. 25:5433-5442(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 34-311.
  10. "Asymmetric structure of the yeast F1 ATPase in the absence of bound nucleotides."
    Kabaleeswaran V., Shen H., Symersky J., Walker J.E., Leslie A.G.W., Mueller D.M.
    J. Biol. Chem. 284:10546-10551(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.59 ANGSTROMS) OF 34-311.

Entry informationi

Entry nameiATPG_YEAST
AccessioniPrimary (citable) accession number: P38077
Secondary accession number(s): D6VQ39, Q54AF5, Q76MT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: May 14, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 28100 molecules/cell in log phase SD medium.
1 Publication reports two tandemly repeated copies of ATP3 (ATP3a and ATP3b) on the right arm of chromosome II in several laboratory strains, including S288c. This has not been confirmed by the yeast genome sequencing project.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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