Reviewed, UniProtKB/Swiss-Prot P38076 (CYSK_WHEAT)

Names and origin

Protein names

Recommended name:
    Cysteine synthase
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase
    CSase A
    O-acetylserine (thiol)-lyase
      Short name=OAS-TL A

Gene names

Name:

CYS1

Organism

Triticum aestivum (Wheat)

Taxonomic identifier

4565 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Cysteine synthase		PRO_0000167126
Regions
	Region	185 – 189	5	Pyridoxal phosphate binding By similarity
	Compositional bias	276 – 281	6	Poly-Ala
Sites
	Binding site	81	1	Pyridoxal phosphate By similarity
	Binding site	273	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	50	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38076-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 1CEF8FE29F341DCA	FASTA	325	34,114
	10 20 30 40 50 60 MGEASSPAIA KDVTELIGNT PLVYLNKVTD GCVGRVAAKL ESMEPCSSVK DRIGYSMITD 70 80 90 100 110 120 AEEKGFIVPG KSVLIEPTSG NTGIGLAFMA AAKGYRLVLT MPASMSMERR IILKAFGAEL 130 140 150 160 170 180 ILTDPLLGMK GAVQKAEELA AKTPNSYILQ QFENAANPKI HYETTGPEIW KGTGGKIDGL 190 200 210 220 230 240 VSGIGTGGTI TGTGKYLQEQ NPNIKLYGVE PTESAILNGG KPGPHKIQGI GAGFIPGVLD 250 260 270 280 290 300 VDIIDETIQV SSDESIEMAK SLALKEGLLV GISSGAAAAA AIKVAQRPEN AGKLFVVVFP 310 320 SFGERYLSSV LFHSIKKEAE SMVVE

References

[1]	"Tobacco plants transformed with the O-acetylserine (thiol) lyase gene of wheat are resistant to toxic levels of hydrogen sulphide gas." Youssefian S., Nakamura M., Sano H. Plant J. 4:759-769(1993) [PubMed: 8275097] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13153 mRNA. Translation: BAA02438.1.
PIR	JS0762.
UniGene	Ta.11201
3D structure databases
HSSP	HSSP built from PDB template 1OAS based on UniProtKB P12674.
SMR	P38076. Positions 9-325.
ModBase	Search...
Organism-specific databases
Gramene	P38076.
Enzyme and pathway databases
BRENDA	2.5.1.47. 253.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01139. cysK. 1 hit. TIGR01136. cysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK_WHEAT

Accession

Primary (citable) accession number: P38076

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)