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P38074 (HMT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HNRNP arginine N-methyltransferase

EC=2.1.1.-
Alternative name(s):
Protein ODP1
Gene names
Name:HMT1
Synonyms:ODP1, RMT1
Ordered Locus Names:YBR034C
ORF Names:YBR0320
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Methylates arginines in a variety of RNA-binding proteins. Methylates NOP3. Can catalyze both the mono- and asymmetric dimethylation. Ref.9

Subunit structure

Homodimer. The dimers can then associate to form a ring-shaped homohexamer. Interacts with NOP3/NPL3. Ref.9

Miscellaneous

Present with 37600 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.

Contains 1 SAM-dependent MTase PRMT-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348HNRNP arginine N-methyltransferase
PRO_0000212341

Regions

Domain20 – 322303SAM-dependent MTase PRMT-type

Sites

Binding site331S-adenosyl-L-methionine By similarity
Binding site421S-adenosyl-L-methionine By similarity
Binding site661S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site881S-adenosyl-L-methionine By similarity
Binding site1171S-adenosyl-L-methionine By similarity

Secondary structure

....................................................... 348
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P38074 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: 383AF61033FB4AC2

FASTA34839,786
        10         20         30         40         50         60 
MSKTAVKDSA TEKTKLSESE QHYFNSYDHY GIHEEMLQDT VRTLSYRNAI IQNKDLFKDK 

        70         80         90        100        110        120 
IVLDVGCGTG ILSMFAAKHG AKHVIGVDMS SIIEMAKELV ELNGFSDKIT LLRGKLEDVH 

       130        140        150        160        170        180 
LPFPKVDIII SEWMGYFLLY ESMMDTVLYA RDHYLVEGGL IFPDKCSIHL AGLEDSQYKD 

       190        200        210        220        230        240 
EKLNYWQDVY GFDYSPFVPL VLHEPIVDTV ERNNVNTTSD KLIEFDLNTV KISDLAFKSN 

       250        260        270        280        290        300 
FKLTAKRQDM INGIVTWFDI VFPAPKGKRP VEFSTGPHAP YTHWKQTIFY FPDDLDAETG 

       310        320        330        340 
DTIEGELVCS PNEKNNRDLN IKISYKFESN GIDGNSRSRK NEGSYLMH 

« Hide

References

« Hide 'large scale' references
[1]"The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
Smits P.H.M., de Haan M., Maat C., Grivell L.A.
Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Sterol uptake induced by an impairment of pyridoxal phosphate synthesis in Saccharomyces cerevisiae: cloning and sequencing of the PDX3 gene encoding pyridoxine (pyridoxamine) phosphate oxidase."
Loubbardi A., Karst F., Guilloton M., Marcireau C.
J. Bacteriol. 177:1817-1823(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55.
[6]Henry M.F., Silver P.A.
Unpublished observations (MAR-1996)
Cited for: CHARACTERIZATION.
[7]"The predominant protein-arginine methyltransferase from Saccharomyces cerevisiae."
Gary J.D., Lin W.-J., Yang M.C., Herschmann H.R., Clarke S.
J. Biol. Chem. 271:12585-12594(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"The structure and oligomerization of the yeast arginine methyltransferase, Hmt1."
Weiss V.H., McBride A.E., Soriano M.A., Filman D.J., Silver P.A., Hogle J.M.
Nat. Struct. Biol. 7:1165-1171(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 24-348, FUNCTION, INTERACTION WITH NOP3/NPL3, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X76078 Genomic DNA. Translation: CAA53689.1.
Z35903 Genomic DNA. Translation: CAA84976.1.
X76992 Genomic DNA. Translation: CAA54296.1.
AY557869 Genomic DNA. Translation: AAS56195.1.
BK006936 Genomic DNA. Translation: DAA07154.1.
PIRS45890.
RefSeqNP_009590.1. NM_001178382.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1G6QX-ray2.901/2/3/4/5/621-348[»]
ProteinModelPortalP38074.
SMRP38074. Positions 28-348.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32735. 300 interactions.
DIPDIP-2608N.
IntActP38074. 265 interactions.
MINTMINT-423994.
STRING4932.YBR034C.

Proteomic databases

MaxQBP38074.
PaxDbP38074.
PeptideAtlasP38074.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR034C; YBR034C; YBR034C.
GeneID852322.
KEGGsce:YBR034C.

Organism-specific databases

CYGDYBR034c.
SGDS000000238. HMT1.

Phylogenomic databases

eggNOGCOG0500.
GeneTreeENSGT00550000074406.
HOGENOMHOG000198521.
KOK11434.
OMADIDFTAC.
OrthoDBEOG7WDNBC.

Enzyme and pathway databases

BioCycYEAST:G3O-29011-MONOMER.

Gene expression databases

GenevestigatorP38074.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR025799. Arg_MeTrfase.
IPR025714. Methyltranfer_dom.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR11006. PTHR11006. 1 hit.
PfamPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS51678. SAM_MT_PRMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP38074.
NextBio971020.

Entry information

Entry nameHMT1_YEAST
AccessionPrimary (citable) accession number: P38074
Secondary accession number(s): D6VQ34
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 9, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references