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P38071

- ETR1_YEAST

UniProt

P38071 - ETR1_YEAST

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Protein

Enoyl-[acyl-carrier protein] reductase [NADPH, B-specific], mitochondrial

Gene
ETR1, MRF1, MRF1', YBR026C, YBR0310
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for respiration and the maintenance of the mitochondrial compartment. Oxidoreductase with a preference for short and medium chain substrates, including trans-2-hexenoyl-CoA (C6), trans-2-decenoyl-CoA (C10), and trans-2-hexadecenoyl-CoA (C16). May play a role in mitochondrial fatty acid synthesis.2 Publications

Catalytic activityi

An acyl-[acyl-carrier protein] + NADP+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH.
Acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei157 – 1571NADP By similarity
Binding sitei373 – 3731NADP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi185 – 1884NADP By similarity
Nucleotide bindingi208 – 2103NADP By similarity
Nucleotide bindingi283 – 2864NADP By similarity
Nucleotide bindingi308 – 3103NADP By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity Source: UniProtKB-EC
  3. enoyl-[acyl-carrier-protein] reductase activity Source: SGD
  4. trans-2-enoyl-CoA reductase (NADPH) activity Source: UniProtKB-EC
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. aerobic respiration Source: SGD
  2. fatty acid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

DNA-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:G3O-29006-MONOMER.
YEAST:G3O-29006-MONOMER.
BRENDAi1.3.1.10. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier protein] reductase [NADPH, B-specific], mitochondrial (EC:1.3.1.10)
Alternative name(s):
Mitochondrial respiratory function protein 1
Trans-2-enoyl-CoA reductase (EC:1.3.1.38)
Gene namesi
Name:ETR1
Synonyms:MRF1, MRF1'
Ordered Locus Names:YBR026C
ORF Names:YBR0310
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBR026c.
SGDiS000000230. ETR1.

Subcellular locationi

Mitochondrion matrix 3 Publications

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi73 – 731Y → N: 0.1% of catalytic activity. No specific ARS1 binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 99Mitochondrion1 Publication
Chaini10 – 380371Enoyl-[acyl-carrier protein] reductase [NADPH, B-specific], mitochondrialPRO_0000160924Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei339 – 3391Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38071.
PaxDbiP38071.
PeptideAtlasiP38071.

Expressioni

Gene expression databases

GenevestigatoriP38071.

Interactioni

Subunit structurei

Homodimer or in a complex with other proteins. Interacts with ARS1.1 Publication

Protein-protein interaction databases

BioGridi32729. 149 interactions.
IntActiP38071. 2 interactions.
MINTiMINT-2788988.
STRINGi4932.YBR026C.

Structurei

3D structure databases

ProteinModelPortaliP38071.
SMRiP38071. Positions 52-377.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0604.
GeneTreeiENSGT00740000115589.
HOGENOMiHOG000294683.
KOiK07512.
OMAiGSGQWFI.
OrthoDBiEOG7PS1RH.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
SUPFAMiSSF50129. SSF50129. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38071-1 [UniParc]FASTAAdd to Basket

« Hide

MLPTFKRYMS SSAHQIPKHF KSLIYSTHEV EDCTKVLSVK NYTPKQDLSQ    50
SIVLKTLAFP INPSDINQLQ GVYPSRPEKT YDYSTDEPAA IAGNEGVFEV 100
VSLPSGSSKG DLKLGDRVIP LQANQGTWSN YRVFSSSSDL IKVNDLDLFS 150
AATVSVNGCT GFQLVSDYID WNSNGNEWII QNAGTSSVSK IVTQVAKAKG 200
IKTLSVIRDR DNFDEVAKVL EDKYGATKVI SESQNNDKTF AKEVLSKILG 250
ENARVRLALN SVGGKSSASI ARKLENNALM LTYGGMSKQP VTLPTSLHIF 300
KGLTSKGYWV TEKNKKNPQS KIDTISDFIK MYNYGHIISP RDEIETLTWN 350
TNTTTDEQLL ELVKKGITGK GKKKMVVLEW 380
Length:380
Mass (Da):42,067
Last modified:October 17, 2006 - v3
Checksum:i9795013283C3E9F8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241I → T in AAS56198. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26606 Genomic DNA. Translation: BAA05651.1.
Z35895 Genomic DNA. Translation: CAA84968.1.
X76078 Genomic DNA. Translation: CAA53683.1.
AY557872 Genomic DNA. Translation: AAS56198.1.
BK006936 Genomic DNA. Translation: DAA07148.1.
RefSeqiNP_009582.1. NM_001178374.1.

Genome annotation databases

EnsemblFungiiYBR026C; YBR026C; YBR026C.
GeneIDi852314.
KEGGisce:YBR026C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D26606 Genomic DNA. Translation: BAA05651.1 .
Z35895 Genomic DNA. Translation: CAA84968.1 .
X76078 Genomic DNA. Translation: CAA53683.1 .
AY557872 Genomic DNA. Translation: AAS56198.1 .
BK006936 Genomic DNA. Translation: DAA07148.1 .
RefSeqi NP_009582.1. NM_001178374.1.

3D structure databases

ProteinModelPortali P38071.
SMRi P38071. Positions 52-377.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32729. 149 interactions.
IntActi P38071. 2 interactions.
MINTi MINT-2788988.
STRINGi 4932.YBR026C.

Proteomic databases

MaxQBi P38071.
PaxDbi P38071.
PeptideAtlasi P38071.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBR026C ; YBR026C ; YBR026C .
GeneIDi 852314.
KEGGi sce:YBR026C.

Organism-specific databases

CYGDi YBR026c.
SGDi S000000230. ETR1.

Phylogenomic databases

eggNOGi COG0604.
GeneTreei ENSGT00740000115589.
HOGENOMi HOG000294683.
KOi K07512.
OMAi GSGQWFI.
OrthoDBi EOG7PS1RH.

Enzyme and pathway databases

BioCyci MetaCyc:G3O-29006-MONOMER.
YEAST:G3O-29006-MONOMER.
BRENDAi 1.3.1.10. 984.

Miscellaneous databases

NextBioi 971002.
PROi P38071.

Gene expression databases

Genevestigatori P38071.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProi IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11695. PTHR11695. 1 hit.
SUPFAMi SSF50129. SSF50129. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A protein which binds preferentially to single-stranded core sequence of autonomously replicating sequence is essential for respiratory function in mitochondrial of Saccharomyces cerevisiae."
    Yamazoe M., Shirahige K., Rashid M.B., Kaneko Y., Nakayama T., Ogasawara N., Yoshikawa H.
    J. Biol. Chem. 269:15244-15252(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 10-25.
    Strain: ATCC 26786 / X2180-1A.
  2. "The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
    Smits P.H.M., de Haan M., Maat C., Grivell L.A.
    Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "Yeast mitochondrial dehydrogenases are associated in a supramolecular complex."
    Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.
    Biochemistry 40:9758-9769(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Candida tropicalis Etr1p and Saccharomyces cerevisiae Ybr026p (Mrf1'p), 2-enoyl thioester reductases essential for mitochondrial respiratory competence."
    Torkko J.M., Koivuranta K.T., Miinalainen I.J., Yagi A.I., Schmitz W., Kastaniotis A.J., Airenne T.T., Gurvitz A., Hiltunen K.J.
    Mol. Cell. Biol. 21:6243-6253(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  8. "Structure-function analysis of enoyl thioester reductase involved in mitochondrial maintenance."
    Airenne T.T., Torkko J.M., Van den plas S., Sormunen R.T., Kastaniotis A.J., Wierenga R.K., Hiltunen J.K.
    J. Mol. Biol. 327:47-59(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TYR-73.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-339, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiETR1_YEAST
AccessioniPrimary (citable) accession number: P38071
Secondary accession number(s): D6VQ28, Q6Q5P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 17, 2006
Last modified: May 14, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1560 molecules/cell in log phase SD medium.

Caution

Was originally (1 Publication) thought to be a nuclear protein involved in transcriptional regulation of genes required for the functional assembly of mitochondrial respiratory proteins. This was later proven not to be the case (1 Publication).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

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