Enoyl-[acyl-carrier protein] reductase [NADPH, B-specific], mitochondrial precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P38071 (ETR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Enoyl-[acyl-carrier protein] reductase [NADPH, B-specific], mitochondrial
EC=1.3.1.10

Alternative name(s):
Mitochondrial respiratory function protein 1
Trans-2-enoyl-CoA reductase
EC=1.3.1.38

Gene names

Name:	ETR1
Synonyms:	MRF1, MRF1'
Ordered Locus Names:	YBR026C
ORF Names:	YBR0310

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for respiration and the maintenance of the mitochondrial compartment. May have a role in the mitochondrial synthesis of fatty acids. Ref.7 Ref.8
Catalytic activity	Acyl-[acyl-carrier-protein] + NADP⁺ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADPH. Acyl-CoA + NADP⁺ = trans-2,3-dehydroacyl-CoA + NADPH.
Subunit structure	Homodimer or in a complex with other proteins. Interacts with ARS1. Ref.7
Subcellular location	Mitochondrion matrix Ref.6 Ref.7 Ref.10.
Miscellaneous	Present with 1560 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
Caution	Was originally (Ref.1) thought to be a nuclear protein involved in transcriptional regulation of genes required for the functional assembly of mitochondrial respiratory proteins. This was later proven not to be the case (Ref.7).

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	DNA-binding NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from mutant phenotype Ref.7. Source: SGD fatty acid biosynthetic process Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity Inferred from electronic annotation. Source: EC enoyl-[acyl-carrier-protein] reductase activity Inferred from direct assay Ref.7. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro trans-2-enoyl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 9

Mitochondrion Ref.1

Chain

10 – 380

371

Enoyl-[acyl-carrier protein] reductase [NADPH, B-specific], mitochondrial

PRO_0000160924

Amino acid modifications

Modified residue

135

Phosphoserine Ref.11

Modified residue

339

Phosphoserine Ref.11

Experimental info

Mutagenesis

Y → N: 0.1% of catalytic activity. No specific ARS1 binding. Ref.8

Sequence conflict

I → T in AAS56198. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P38071 [UniParc].

Last modified October 17, 2006. Version 3.
Checksum: 9795013283C3E9F8
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FASTA

380

42,067

        10         20         30         40         50         60 
MLPTFKRYMS SSAHQIPKHF KSLIYSTHEV EDCTKVLSVK NYTPKQDLSQ SIVLKTLAFP 

        70         80         90        100        110        120 
INPSDINQLQ GVYPSRPEKT YDYSTDEPAA IAGNEGVFEV VSLPSGSSKG DLKLGDRVIP 

       130        140        150        160        170        180 
LQANQGTWSN YRVFSSSSDL IKVNDLDLFS AATVSVNGCT GFQLVSDYID WNSNGNEWII 

       190        200        210        220        230        240 
QNAGTSSVSK IVTQVAKAKG IKTLSVIRDR DNFDEVAKVL EDKYGATKVI SESQNNDKTF 

       250        260        270        280        290        300 
AKEVLSKILG ENARVRLALN SVGGKSSASI ARKLENNALM LTYGGMSKQP VTLPTSLHIF 

       310        320        330        340        350        360 
KGLTSKGYWV TEKNKKNPQS KIDTISDFIK MYNYGHIISP RDEIETLTWN TNTTTDEQLL 

       370        380 
ELVKKGITGK GKKKMVVLEW

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A protein which binds preferentially to single-stranded core sequence of autonomously replicating sequence is essential for respiratory function in mitochondrial of Saccharomyces cerevisiae." Yamazoe M., Shirahige K., Rashid M.B., Kaneko Y., Nakayama T., Ogasawara N., Yoshikawa H. J. Biol. Chem. 269:15244-15252(1994) [PubMed: 8195160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 10-25. Strain: ATCC 26786 / X2180-1A.
[2]	"The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene." Smits P.H.M., de Haan M., Maat C., Grivell L.A. Yeast 10:S75-S80(1994) [PubMed: 8091864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex." Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M. Biochemistry 40:9758-9769(2001) [PubMed: 11502169] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Candida tropicalis Etr1p and Saccharomyces cerevisiae Ybr026p (Mrf1'p), 2-enoyl thioester reductases essential for mitochondrial respiratory competence." Torkko J.M., Koivuranta K.T., Miinalainen I.J., Yagi A.I., Schmitz W., Kastaniotis A.J., Airenne T.T., Gurvitz A., Hiltunen K.J. Mol. Cell. Biol. 21:6243-6253(2001) [PubMed: 11509667] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[8]	"Structure-function analysis of enoyl thioester reductase involved in mitochondrial maintenance." Airenne T.T., Torkko J.M., Van den plas S., Sormunen R.T., Kastaniotis A.J., Wierenga R.K., Hiltunen J.K. J. Mol. Biol. 327:47-59(2003) [PubMed: 12614607] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF TYR-73.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-135 AND SER-339, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26606 Genomic DNA. Translation: BAA05651.1. Z35895 Genomic DNA. Translation: CAA84968.1. X76078 Genomic DNA. Translation: CAA53683.1. AY557872 Genomic DNA. Translation: AAS56198.1. BK006936 Genomic DNA. Translation: DAA07148.1.
RefSeq	NP_009582.1. NM_001178374.1.
3D structure databases
ProteinModelPortal	P38071.
SMR	P38071. Positions 19-377.
ModBase	Search...
Protein-protein interaction databases
IntAct	P38071. 2 interactions.
MINT	MINT-2788988.
STRING	P38071.
Proteomic databases
PeptideAtlas	P38071.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR026C; YBR026C; YBR026C.
GeneID	852314.
KEGG	sce:YBR026C.
NMPDR	fig\|4932.3.peg.277.
Organism-specific databases
CYGD	YBR026c.
SGD	S000000230. ETR1.
Phylogenomic databases
eggNOG	fuNOG07814.
GeneTree	EFGT00050000001806.
HOGENOM	HBG753318.
OMA	QANQGTW.
OrthoDB	EOG4D29ZS.
Enzyme and pathway databases
BRENDA	1.3.1.10. 984.
Gene expression databases
ArrayExpress	P38071.
Genevestigator	P38071.
GermOnline	YBR026C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002085. ADH_SF_Zn-type. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K07512.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
SUPFAM	SSF50129. GroES_like. 1 hit.
ProtoNet	Search...
Other
NextBio	971002.

Entry information

Entry name

ETR1_YEAST

Accession

Primary (citable) accession number: P38071
Secondary accession number(s): D6VQ28, Q6Q5P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 17, 2006
Last modified:	December 14, 2011
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents