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Protein

Serine/threonine-protein kinase YPK3

Gene

YPK3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

AGC kinase which plays a role in TOR complex 1 (TORC1) signaling pathway which mediates temporal control of cell growth in response to nutrients (PubMed:11062466). Required for phosphorylation of ribosomal protein S6 (RPS6A/RPS6B) at 'Ser-232' and 'Ser-233' (PubMed:25767889).2 Publications

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei157ATPPROSITE-ProRule annotation1
Active sitei277Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi134 – 142ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: SGD
  • protein autophosphorylation Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29007-MONOMER.
ReactomeiR-SCE-111933. Calmodulin induced events.
R-SCE-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase YPK31 Publication (EC:2.7.11.11 Publication)
Alternative name(s):
Ribosomal S6 kinase homolog YPK31 Publication
Short name:
S6K homolog YPK3
Gene namesi
Name:YPK31 Publication
Ordered Locus Names:YBR028CImported
ORF Names:YBR0312
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR028C.
SGDiS000000232. YPK3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860731 – 525Serine/threonine-protein kinase YPK3Add BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei90PhosphoserineCombined sources1
Modified residuei105PhosphoserineCombined sources1
Modified residuei107PhosphothreonineCombined sources1
Modified residuei321Phosphoserine; by PKH1 or PKH2By similarity1
Modified residuei490Phosphothreonine; by TORC1By similarity1
Modified residuei513Phosphoserine; by TORC1By similarity1

Post-translational modificationi

Phosphorylated by PKA in a TORC1-dependent manner. Phosphorylation at PKA consensus sites RRxS/T decreases upon rapamycin treatment.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38070.
PRIDEiP38070.

PTM databases

iPTMnetiP38070.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
NDC80P404602EBI-9524,EBI-25247

Protein-protein interaction databases

BioGridi32730. 76 interactors.
DIPiDIP-4629N.
IntActiP38070. 12 interactors.
MINTiMINT-509244.
STRINGi4932.YBR028C.

Structurei

3D structure databases

ProteinModelPortaliP38070.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini128 – 424Protein kinasePROSITE-ProRule annotationAdd BLAST297
Domaini445 – 524AGC-kinase C-terminalAdd BLAST80

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00890000140606.
InParanoidiP38070.
KOiK08286.
OrthoDBiEOG092C0HH3.

Family and domain databases

InterProiView protein in InterPro
IPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
SMARTiView protein in SMART
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiView protein in PROSITE
PS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

P38070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIFSLDEELH RVSLDDKKND IKVDYSSAIY NDINHEQGSS ITYEESINHL
60 70 80 90 100
SVHSNAIPLN GMSPAHRMRR RSSAYSKFPI LTPPNTRRFS ITGSDAMRTN
110 120 130 140 150
TNRLSITPQD IISSNIGENE LSRNLHDFKP VRVLGQGAYG KVLLVKDVNT
160 170 180 190 200
SKLYAMKQLR KAEILISQTA TDSKREDEDK NDGNNNDNDD GLSKRLERTF
210 220 230 240 250
AERSILSEIE HPNIVKLFYS FHDNSKLYLL LQYIPGGELF YHLKEHGTLD
260 270 280 290 300
ETTVSFYAAE ISCALRFLHT KGVVYRDLKP ENCLLNQRGH LVLTDFGLSK
310 320 330 340 350
KSANDSAVDE EDPENVNALY SIIGTPEYCA PEILLGKAYS QNCDWYSLGC
360 370 380 390 400
LLYDMLVGKP PYTGSNHKVI INKIQQNKQG PKIPFYLSEG MKDILNALLK
410 420 430 440 450
KETAKRWNVD KYWAKTGANN KPTKSKKKKS GAARTSLFTE HFIFRKIDWK
460 470 480 490 500
LLESGQLQKT TLGPIVPVIT DLELAENFDT EFTSMSYEET YTDSKPININ
510 520
SVSKSPDMFK GFSYKASGSY LEKYF
Length:525
Mass (Da):59,591
Last modified:October 1, 1994 - v1
Checksum:iB8FFE61A44067677
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76078 Genomic DNA. Translation: CAA53684.1.
Z35897 Genomic DNA. Translation: CAA84970.1.
BK006936 Genomic DNA. Translation: DAA07149.1.
PIRiS45884.
RefSeqiNP_009584.1. NM_001178376.1.

Genome annotation databases

EnsemblFungiiYBR028C; YBR028C; YBR028C.
GeneIDi852316.
KEGGisce:YBR028C.

Similar proteinsi

Entry informationi

Entry nameiYPK3_YEAST
AccessioniPrimary (citable) accession number: P38070
Secondary accession number(s): D6VQ29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 27, 2017
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names