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P38070 (YPK3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AGC kinase YPK3

EC=2.7.11.1
Gene names
Name:YPK3
Ordered Locus Names:YBR028C
ORF Names:YBR0312
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

AGC kinase which plays a role in TOR complex 1 (TORC1) signaling pathway which mediates temporal control of cell growth in response to nutrients. Ref.4

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subcellular location

Cytoplasm Ref.5.

Post-translational modification

Phosphorylated by PKA in a TORC1-dependent manner. Phosphorylation at PKA consensus sites RRxS/T decreases upon rapamycin treatment. Ref.8 Ref.12

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525AGC kinase YPK3
PRO_0000086073

Regions

Domain128 – 424297Protein kinase
Domain445 – 52480AGC-kinase C-terminal
Nucleotide binding134 – 1429ATP By similarity

Sites

Active site2771Proton acceptor By similarity
Binding site1571ATP By similarity

Amino acid modifications

Modified residue901Phosphoserine Ref.7 Ref.9
Modified residue1051Phosphoserine Ref.11
Modified residue1071Phosphothreonine Ref.11

Sequences

Sequence LengthMass (Da)Tools
P38070 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: B8FFE61A44067677

FASTA52559,591
        10         20         30         40         50         60 
MIFSLDEELH RVSLDDKKND IKVDYSSAIY NDINHEQGSS ITYEESINHL SVHSNAIPLN 

        70         80         90        100        110        120 
GMSPAHRMRR RSSAYSKFPI LTPPNTRRFS ITGSDAMRTN TNRLSITPQD IISSNIGENE 

       130        140        150        160        170        180 
LSRNLHDFKP VRVLGQGAYG KVLLVKDVNT SKLYAMKQLR KAEILISQTA TDSKREDEDK 

       190        200        210        220        230        240 
NDGNNNDNDD GLSKRLERTF AERSILSEIE HPNIVKLFYS FHDNSKLYLL LQYIPGGELF 

       250        260        270        280        290        300 
YHLKEHGTLD ETTVSFYAAE ISCALRFLHT KGVVYRDLKP ENCLLNQRGH LVLTDFGLSK 

       310        320        330        340        350        360 
KSANDSAVDE EDPENVNALY SIIGTPEYCA PEILLGKAYS QNCDWYSLGC LLYDMLVGKP 

       370        380        390        400        410        420 
PYTGSNHKVI INKIQQNKQG PKIPFYLSEG MKDILNALLK KETAKRWNVD KYWAKTGANN 

       430        440        450        460        470        480 
KPTKSKKKKS GAARTSLFTE HFIFRKIDWK LLESGQLQKT TLGPIVPVIT DLELAENFDT 

       490        500        510        520 
EFTSMSYEET YTDSKPININ SVSKSPDMFK GFSYKASGSY LEKYF 

« Hide

References

« Hide 'large scale' references
[1]"The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
Smits P.H.M., de Haan M., Maat C., Grivell L.A.
Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome II."
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. expand/collapse author list , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Analysis of yeast protein kinases using protein chips."
Zhu H., Klemic J.F., Chang S., Bertone P., Casamayor A., Klemic K.G., Smith D., Gerstein M., Reed M.A., Snyder M.
Nat. Genet. 26:283-289(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[8]"An evolutionary proteomics approach identifies substrates of the cAMP-dependent protein kinase."
Budovskaya Y.V., Stephan J.S., Deminoff S.J., Herman P.K.
Proc. Natl. Acad. Sci. U.S.A. 102:13933-13938(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY PKA.
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105 AND THR-107, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"The rapamycin-sensitive phosphoproteome reveals that TOR controls protein kinase A toward some but not all substrates."
Soulard A., Cremonesi A., Moes S., Schutz F., Jeno P., Hall M.N.
Mol. Biol. Cell 21:3475-3486(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY PKA.
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X76078 Genomic DNA. Translation: CAA53684.1.
Z35897 Genomic DNA. Translation: CAA84970.1.
BK006936 Genomic DNA. Translation: DAA07149.1.
PIRS45884.
RefSeqNP_009584.1. NM_001178376.1.

3D structure databases

ProteinModelPortalP38070.
SMRP38070. Positions 128-516.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32730. 32 interactions.
DIPDIP-4629N.
IntActP38070. 4 interactions.
MINTMINT-509244.
STRING4932.YBR028C.

Proteomic databases

PaxDbP38070.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYBR028C; YBR028C; YBR028C.
GeneID852316.
KEGGsce:YBR028C.

Organism-specific databases

CYGDYBR028c.
SGDS000000232. YPK3.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00750000117841.
KOK08286.
OMAIGTPEYC.
OrthoDBEOG7R2BT5.

Enzyme and pathway databases

BioCycYEAST:G3O-29007-MONOMER.

Gene expression databases

GenevestigatorP38070.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 2 hits.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio971005.

Entry information

Entry nameYPK3_YEAST
AccessionPrimary (citable) accession number: P38070
Secondary accession number(s): D6VQ29
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families