Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase YPK3

Gene

YPK3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

AGC kinase which plays a role in TOR complex 1 (TORC1) signaling pathway which mediates temporal control of cell growth in response to nutrients (PubMed:11062466). Required for phosphorylation of ribosomal protein S6 (RPS6A/RPS6B) at 'Ser-232' and 'Ser-233' (PubMed:25767889).2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei157 – 1571ATPPROSITE-ProRule annotation
Active sitei277 – 2771Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi134 – 1429ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: SGD
  • protein autophosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29007-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase YPK31 Publication (EC:2.7.11.11 Publication)
Alternative name(s):
Ribosomal S6 kinase homolog YPK31 Publication
Short name:
S6K homolog YPK3
Gene namesi
Name:YPK31 Publication
Ordered Locus Names:YBR028CImported
ORF Names:YBR0312
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR028C.
SGDiS000000232. YPK3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Serine/threonine-protein kinase YPK3PRO_0000086073Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei90 – 901PhosphoserineCombined sources
Modified residuei105 – 1051PhosphoserineCombined sources
Modified residuei107 – 1071PhosphothreonineCombined sources
Modified residuei321 – 3211Phosphoserine; by PKH1 or PKH2By similarity
Modified residuei490 – 4901Phosphothreonine; by TORC1By similarity
Modified residuei513 – 5131Phosphoserine; by TORC1By similarity

Post-translational modificationi

Phosphorylated by PKA in a TORC1-dependent manner. Phosphorylation at PKA consensus sites RRxS/T decreases upon rapamycin treatment.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38070.

PTM databases

iPTMnetiP38070.

Interactioni

Protein-protein interaction databases

BioGridi32730. 36 interactions.
DIPiDIP-4629N.
IntActiP38070. 5 interactions.
MINTiMINT-509244.

Structurei

3D structure databases

ProteinModelPortaliP38070.
SMRiP38070. Positions 80-516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini128 – 424297Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini445 – 52480AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00800000124105.
InParanoidiP38070.
KOiK08286.
OMAiCVESANE.
OrthoDBiEOG7R2BT5.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P38070-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIFSLDEELH RVSLDDKKND IKVDYSSAIY NDINHEQGSS ITYEESINHL
60 70 80 90 100
SVHSNAIPLN GMSPAHRMRR RSSAYSKFPI LTPPNTRRFS ITGSDAMRTN
110 120 130 140 150
TNRLSITPQD IISSNIGENE LSRNLHDFKP VRVLGQGAYG KVLLVKDVNT
160 170 180 190 200
SKLYAMKQLR KAEILISQTA TDSKREDEDK NDGNNNDNDD GLSKRLERTF
210 220 230 240 250
AERSILSEIE HPNIVKLFYS FHDNSKLYLL LQYIPGGELF YHLKEHGTLD
260 270 280 290 300
ETTVSFYAAE ISCALRFLHT KGVVYRDLKP ENCLLNQRGH LVLTDFGLSK
310 320 330 340 350
KSANDSAVDE EDPENVNALY SIIGTPEYCA PEILLGKAYS QNCDWYSLGC
360 370 380 390 400
LLYDMLVGKP PYTGSNHKVI INKIQQNKQG PKIPFYLSEG MKDILNALLK
410 420 430 440 450
KETAKRWNVD KYWAKTGANN KPTKSKKKKS GAARTSLFTE HFIFRKIDWK
460 470 480 490 500
LLESGQLQKT TLGPIVPVIT DLELAENFDT EFTSMSYEET YTDSKPININ
510 520
SVSKSPDMFK GFSYKASGSY LEKYF
Length:525
Mass (Da):59,591
Last modified:October 1, 1994 - v1
Checksum:iB8FFE61A44067677
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76078 Genomic DNA. Translation: CAA53684.1.
Z35897 Genomic DNA. Translation: CAA84970.1.
BK006936 Genomic DNA. Translation: DAA07149.1.
PIRiS45884.
RefSeqiNP_009584.1. NM_001178376.1.

Genome annotation databases

EnsemblFungiiYBR028C; YBR028C; YBR028C.
GeneIDi852316.
KEGGisce:YBR028C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76078 Genomic DNA. Translation: CAA53684.1.
Z35897 Genomic DNA. Translation: CAA84970.1.
BK006936 Genomic DNA. Translation: DAA07149.1.
PIRiS45884.
RefSeqiNP_009584.1. NM_001178376.1.

3D structure databases

ProteinModelPortaliP38070.
SMRiP38070. Positions 80-516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32730. 36 interactions.
DIPiDIP-4629N.
IntActiP38070. 5 interactions.
MINTiMINT-509244.

PTM databases

iPTMnetiP38070.

Proteomic databases

MaxQBiP38070.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR028C; YBR028C; YBR028C.
GeneIDi852316.
KEGGisce:YBR028C.

Organism-specific databases

EuPathDBiFungiDB:YBR028C.
SGDiS000000232. YPK3.

Phylogenomic databases

GeneTreeiENSGT00800000124105.
InParanoidiP38070.
KOiK08286.
OMAiCVESANE.
OrthoDBiEOG7R2BT5.

Enzyme and pathway databases

BioCyciYEAST:G3O-29007-MONOMER.

Miscellaneous databases

PROiP38070.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene."
    Smits P.H.M., de Haan M., Maat C., Grivell L.A.
    Yeast 10:S75-S80(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: FUNCTION, CATALYTIC ACTIVITY.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "An evolutionary proteomics approach identifies substrates of the cAMP-dependent protein kinase."
    Budovskaya Y.V., Stephan J.S., Deminoff S.J., Herman P.K.
    Proc. Natl. Acad. Sci. U.S.A. 102:13933-13938(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY PKA.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-90, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105 AND THR-107, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "The rapamycin-sensitive phosphoproteome reveals that TOR controls protein kinase A toward some but not all substrates."
    Soulard A., Cremonesi A., Moes S., Schutz F., Jeno P., Hall M.N.
    Mol. Biol. Cell 21:3475-3486(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY PKA.
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "TORC1 promotes phosphorylation of ribosomal protein S6 via the AGC kinase Ypk3 in Saccharomyces cerevisiae."
    Gonzalez A., Shimobayashi M., Eisenberg T., Merle D.A., Pendl T., Hall M.N., Moustafa T.
    PLoS ONE 10:E0120250-E0120250(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiYPK3_YEAST
AccessioniPrimary (citable) accession number: P38070
Secondary accession number(s): D6VQ29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: July 6, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.