Reviewed, UniProtKB/Swiss-Prot P38069 (TTP1_YEAST)

Names and origin

Protein names

Recommended name:
    Protein TTP1
Alternative name(s):
    Mannan synthesis protein MNN2

Gene names

Name:	TTP1
Synonyms:	MNN2
Ordered Locus Names:	YBR015C
ORF Names:	YBR0220

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Miscellaneous	Present with 6730 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	To yeast YJL186w.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from mutant phenotype. Source: SGD
Cellular component	Golgi apparatus Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,2-mannosyltransferase activity Inferred from mutant phenotype. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SAM50	P53969	1	EBI-2072207,EBI-28646

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 597	597	Protein TTP1		PRO_0000065676
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 28	16	Signal-anchor for type II membrane protein Potential
	Topological domain	29 – 597	569	Extracellular Potential
Amino acid modifications
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Glycosylation	363	1	N-linked (GlcNAc...) Potential
	Glycosylation	473	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	287	1	T → K in AAA21860. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38069-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 6C4FA1F4692A261C	FASTA	597	67,774
	10 20 30 40 50 60 MLLTKRFSKL FKLTFIVLIL CGLFVITNKY MDENTSVKEY KEYLDRYVQS YSNKYSSSSD 70 80 90 100 110 120 AASADDSTPL RDNDEAGNEK LKSFYNNVFN FLMVDSPKGS TAKQYNEACL LKGDIGDRPD 130 140 150 160 170 180 HYKDLYKLSA KELSKCLELS PDEVASLTKS HKDYVEHIAT LVSPKGTYKG SGIATVGGGK 190 200 210 220 230 240 FSLMAFLIIK TLRNMGTTLP VEVLIPPGDE GETEFCNKIL PKYNSKCIYV SDILPRETIE 250 260 270 280 290 300 KFVFKGYQFK SLALIASSFE NLLLLDADNF PIKPLDNIFN EEPYVSTGLV MWPDFWRRTT 310 320 330 340 350 360 HPLYYDIAGI AVDKKKRVRN SRDDITPPAV YTKDLKDLSD VPLSDLDGTI PDVSTESGQL 370 380 390 400 410 420 MINKTKHLAT ALLSLFYNVN GPTWYYPIFS QKAAGEGDKE TFIAAANFYG LSFYQVRTRT 430 440 450 460 470 480 GVEGYHDEDG FHGVAMLQHD FVQDYGRYLN AMESIGNKYG GTKSADAIKF DKNYSLEKYT 490 500 510 520 530 540 EEFFDNEDLN AKNHVDVMFI HSNFPKFDPY DLSKSNFLTT NGKPARSYTA LKKVKNYDIE 550 560 570 580 590 LENFKVLNEY VCVNKNPFKY LDDLLGQDKT EWKRVCGYIT DRLAFLESTH DKAIAGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of TTP1, a gene encoding a predicted type II membrane protein." Romero P.A., Athanassiadis A., Lussier M., Herscovics A. Yeast 10:1111-1115(1994) [PubMed: 7992511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U05211 Genomic DNA. Translation: AAA21860.1. Z35884 Genomic DNA. Translation: CAA84957.1.
PIR	S45870.
RefSeq	NP_009571.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:7625N.
IntAct	P38069. 1 interaction.
Protein family/group databases
CAZy	GT71. Glycosyltransferase Family 71.
Proteomic databases
PeptideAtlas	P38069.
Genome annotation databases
Ensembl	YBR015C. Saccharomyces cerevisiae. [Contig view]
GeneID	852303.
GenomeReviews	Gene locus YBR015C in contig Y13134_GR.
KEGG	sce:YBR015C.
NMPDR	fig|4932.3.peg.266.
Organism-specific databases
CYGD	YBR015c.
SGD	S000000219. MNN2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P38069.
OMA	P38069. MEGTLPD.
Gene expression databases
ArrayExpress	P38069.
GermOnline	YBR015C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	970969.

Entry information

Entry name

TTP1_YEAST

Accession

Primary (citable) accession number: P38069

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)