P38069 (MNN2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,2-mannosyltransferase MNN2
EC=2.4.1.-

Alternative name(s):
Calcium resistance and vanadate sensitivity protein 4
Mannan synthesis protein MNN2

Gene names

Name:	MNN2
Synonyms:	CRV4, LDB8, TTP1
Ordered Locus Names:	YBR015C
ORF Names:	YBR0220

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.15
Pathway	Protein modification; protein glycosylation.
Subunit structure	Interacts with SVP26. Ref.19
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein. Note: Golgi localization depends on SVP26. Ref.13 Ref.15 Ref.17 Ref.18 Ref.19
Disruption phenotype	Leads to calcium resistance and vanadate sensitivity. Ref.14
Miscellaneous	Present with 6730 molecules/cell in log phase SD medium. Ref.16
Sequence similarities	Belongs to the MNN1/MNT family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein glycosylation Inferred from mutant phenotype Ref.15. Source: SGD
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,2-mannosyltransferase activity Inferred from mutant phenotype Ref.15. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SAM50	P53969	1	EBI-2072207,EBI-28646

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 597	597	Alpha-1,2-mannosyltransferase MNN2		PRO_0000065676
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 28	16	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	29 – 597	569	Extracellular Potential
Amino acid modifications
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Glycosylation	363	1	N-linked (GlcNAc...) Potential
	Glycosylation	473	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	287	1	T → K in AAA21860. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38069 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 6C4FA1F4692A261C	FASTA	597	67,774
	10 20 30 40 50 60 MLLTKRFSKL FKLTFIVLIL CGLFVITNKY MDENTSVKEY KEYLDRYVQS YSNKYSSSSD 70 80 90 100 110 120 AASADDSTPL RDNDEAGNEK LKSFYNNVFN FLMVDSPKGS TAKQYNEACL LKGDIGDRPD 130 140 150 160 170 180 HYKDLYKLSA KELSKCLELS PDEVASLTKS HKDYVEHIAT LVSPKGTYKG SGIATVGGGK 190 200 210 220 230 240 FSLMAFLIIK TLRNMGTTLP VEVLIPPGDE GETEFCNKIL PKYNSKCIYV SDILPRETIE 250 260 270 280 290 300 KFVFKGYQFK SLALIASSFE NLLLLDADNF PIKPLDNIFN EEPYVSTGLV MWPDFWRRTT 310 320 330 340 350 360 HPLYYDIAGI AVDKKKRVRN SRDDITPPAV YTKDLKDLSD VPLSDLDGTI PDVSTESGQL 370 380 390 400 410 420 MINKTKHLAT ALLSLFYNVN GPTWYYPIFS QKAAGEGDKE TFIAAANFYG LSFYQVRTRT 430 440 450 460 470 480 GVEGYHDEDG FHGVAMLQHD FVQDYGRYLN AMESIGNKYG GTKSADAIKF DKNYSLEKYT 490 500 510 520 530 540 EEFFDNEDLN AKNHVDVMFI HSNFPKFDPY DLSKSNFLTT NGKPARSYTA LKKVKNYDIE 550 560 570 580 590 LENFKVLNEY VCVNKNPFKY LDDLLGQDKT EWKRVCGYIT DRLAFLESTH DKAIAGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of TTP1, a gene encoding a predicted type II membrane protein." Romero P.A., Athanassiadis A., Lussier M., Herscovics A. Yeast 10:1111-1115(1994) [PubMed: 7992511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Genetic control of yeast mannan structure. Isolation and characterization of mannan mutants." Raschke W.C., Kern K.A., Antalis C., Ballou C.E. J. Biol. Chem. 248:4660-4666(1973) [PubMed: 4578088] [Abstract] Cited for: FUNCTION.
[5]	"Genetic control of yeast mannan structure. Complementation studies and properties of mannan mutants." Ballou C.E., Kern K.A., Raschke W.C. J. Biol. Chem. 248:4667-4671(1973) [PubMed: 4124122] [Abstract] Cited for: FUNCTION.
[6]	"A comparison of yeast mannan mutants by electron microscopy." Hawkins E.R. J. Biol. Chem. 248:4671-4673(1973) [PubMed: 4578089] [Abstract] Cited for: FUNCTION.
[7]	"Characterization of the carbohydrate fragments obtained from Saccharomyces cerevisiae mannan by alkaline degradation." Nakajima T., Ballou C.E. J. Biol. Chem. 249:7679-7684(1974) [PubMed: 4612040] [Abstract] Cited for: FUNCTION.
[8]	"Structure of the linkage region between the polysaccharide and protein parts of Saccharomyces cerevisiae mannan." Nakajima T., Ballou C.E. J. Biol. Chem. 249:7685-7694(1974) [PubMed: 4612041] [Abstract] Cited for: FUNCTION.
[9]	"Genetic control of yeast mannan structure: mapping genes mnn2 and mnn4 in Saccharomyces cerevisiae." Ballou D.L. J. Bacteriol. 123:616-619(1975) [PubMed: 1097420] [Abstract] Cited for: FUNCTION.
[10]	"Biosynthesis of yeast mannan. Properties of a mannosylphosphate transferase in Saccharomyces cerevisiae." Karson E.M., Ballou C.E. J. Biol. Chem. 253:6484-6492(1978) [PubMed: 355255] [Abstract] Cited for: FUNCTION.
[11]	"Biosynthesis of yeast mannoproteins. Synthesis of mannan outer chain and of dolichol derivatives." Parodi A.J. J. Biol. Chem. 254:8343-8352(1979) [PubMed: 381304] [Abstract] Cited for: FUNCTION.
[12]	"Effects of mannoprotein mutations on Saccharomyces cerevisiae core oligosaccharide structure." Cohen R.E., Zhang W., Ballou C.E. J. Biol. Chem. 257:5730-5737(1982) [PubMed: 6802821] [Abstract] Cited for: FUNCTION.
[13]	"Identification and characterization of a gene and protein required for glycosylation in the yeast Golgi." Devlin C., Ballou C.E. Mol. Microbiol. 4:1993-2001(1990) [PubMed: 2082155] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[14]	"Yeast Crv4/Ttp1, a predicted type II membrane protein, is involved in an event important for growth, functionally overlapping with the event regulated by calcineurin- and Mpk1-mediated pathways." Nakamura T., Ohmoto T., Hirata D., Tsuchiya E., Miyakawa T. Mol. Gen. Genet. 256:481-487(1997) [PubMed: 9413431] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[15]	"Identification of the MNN2 and MNN5 mannosyltransferases required for forming and extending the mannose branches of the outer chain mannans of Saccharomyces cerevisiae." Rayner J.C., Munro S. J. Biol. Chem. 273:26836-26843(1998) [PubMed: 9756928] [Abstract] Cited for: FUNCTION, GLYCOLSYLATION, SUBCELLULAR LOCATION.
[16]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[17]	"Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments." Inadome H., Noda Y., Adachi H., Yoda K. Mol. Cell. Biol. 25:7696-7710(2005) [PubMed: 16107716] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[18]	"Golgi localization of glycosyltransferases requires a Vps74p oligomer." Schmitz K.R., Liu J., Li S., Setty T.G., Wood C.S., Burd C.G., Ferguson K.M. Dev. Cell 14:523-534(2008) [PubMed: 18410729] [Abstract] Cited for: SUBCELLULAR LOCATION.
[19]	"Svp26 facilitates endoplasmic reticulum to Golgi transport of a set of mannosyltransferases in Saccharomyces cerevisiae." Noda Y., Yoda K. J. Biol. Chem. 285:15420-15429(2010) [PubMed: 20236934] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SVP26.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U05211 Genomic DNA. Translation: AAA21860.1. Z35884 Genomic DNA. Translation: CAA84957.1. BK006936 Genomic DNA. Translation: DAA07137.1.
PIR	S45870.
RefSeq	NP_009571.1. NM_001178363.1.
3D structure databases
ProteinModelPortal	P38069.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7625N.
IntAct	P38069. 1 interaction.
STRING	P38069.
Protein family/group databases
CAZy	GT71. Glycosyltransferase Family 71.
Proteomic databases
PeptideAtlas	P38069.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR015C; YBR015C; YBR015C.
GeneID	852303.
KEGG	sce:YBR015C.
NMPDR	fig|4932.3.peg.266.
Organism-specific databases
CYGD	YBR015c.
SGD	S000000219. MNN2.
Phylogenomic databases
eggNOG	fuNOG04522.
GeneTree	EFGT00050000003822.
HOGENOM	HBG204123.
OMA	HDMEGTL.
OrthoDB	EOG4CRQ7M.
Gene expression databases
ArrayExpress	P38069.
Genevestigator	P38069.
GermOnline	YBR015C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022751. Alpha_mannosyltransferase. [Graphical view]
KO	K05535.
Pfam	PF11051. Mannosyl_trans3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970969.

Entry information

Entry name

MNN2_YEAST

Accession

Primary (citable) accession number: P38069
Secondary accession number(s): D6VQ17

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program