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Protein

Alpha-1,2-mannosyltransferase MNN2

Gene

MNN2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides.11 Publications

Miscellaneous

Present with 6730 molecules/cell in log phase SD medium.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • alpha-1,2-mannosyltransferase activity Source: SGD

GO - Biological processi

  • mannan biosynthetic process Source: GO_Central
  • protein glycosylation Source: SGD
  • protein mannosylation Source: GO_Central

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BioCyciYEAST:YBR015C-MONOMER.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT71. Glycosyltransferase Family 71.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,2-mannosyltransferase MNN2 (EC:2.4.1.-)
Alternative name(s):
Calcium resistance and vanadate sensitivity protein 4
Mannan synthesis protein MNN2
Gene namesi
Name:MNN2
Synonyms:CRV4, LDB8, TTP1
Ordered Locus Names:YBR015C
ORF Names:YBR0220
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR015C.
SGDiS000000219. MNN2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST16
Topological domaini29 – 597ExtracellularSequence analysisAdd BLAST569

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Leads to calcium resistance and vanadate sensitivity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000656761 – 597Alpha-1,2-mannosyltransferase MNN2Add BLAST597

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi34N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi363N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi473N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP38069.
PRIDEiP38069.

Interactioni

Subunit structurei

Interacts with SVP26.1 Publication

Protein-protein interaction databases

BioGridi32718. 227 interactors.
DIPiDIP-7625N.
IntActiP38069. 4 interactors.
MINTiMINT-4476654.
STRINGi4932.YBR015C.

Structurei

3D structure databases

ProteinModelPortaliP38069.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MNN1/MNT family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000068016.
InParanoidiP38069.
KOiK05535.
OMAiLIVWPDF.
OrthoDBiEOG092C13WV.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR022751. Alpha_mannosyltransferase.
IPR029044. Nucleotide-diphossugar_trans.
PfamiView protein in Pfam
PF11051. Mannosyl_trans3. 1 hit.
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

P38069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLTKRFSKL FKLTFIVLIL CGLFVITNKY MDENTSVKEY KEYLDRYVQS
60 70 80 90 100
YSNKYSSSSD AASADDSTPL RDNDEAGNEK LKSFYNNVFN FLMVDSPKGS
110 120 130 140 150
TAKQYNEACL LKGDIGDRPD HYKDLYKLSA KELSKCLELS PDEVASLTKS
160 170 180 190 200
HKDYVEHIAT LVSPKGTYKG SGIATVGGGK FSLMAFLIIK TLRNMGTTLP
210 220 230 240 250
VEVLIPPGDE GETEFCNKIL PKYNSKCIYV SDILPRETIE KFVFKGYQFK
260 270 280 290 300
SLALIASSFE NLLLLDADNF PIKPLDNIFN EEPYVSTGLV MWPDFWRRTT
310 320 330 340 350
HPLYYDIAGI AVDKKKRVRN SRDDITPPAV YTKDLKDLSD VPLSDLDGTI
360 370 380 390 400
PDVSTESGQL MINKTKHLAT ALLSLFYNVN GPTWYYPIFS QKAAGEGDKE
410 420 430 440 450
TFIAAANFYG LSFYQVRTRT GVEGYHDEDG FHGVAMLQHD FVQDYGRYLN
460 470 480 490 500
AMESIGNKYG GTKSADAIKF DKNYSLEKYT EEFFDNEDLN AKNHVDVMFI
510 520 530 540 550
HSNFPKFDPY DLSKSNFLTT NGKPARSYTA LKKVKNYDIE LENFKVLNEY
560 570 580 590
VCVNKNPFKY LDDLLGQDKT EWKRVCGYIT DRLAFLESTH DKAIAGK
Length:597
Mass (Da):67,774
Last modified:October 1, 1994 - v1
Checksum:i6C4FA1F4692A261C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti287T → K in AAA21860 (PubMed:7992511).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05211 Genomic DNA. Translation: AAA21860.1.
Z35884 Genomic DNA. Translation: CAA84957.1.
BK006936 Genomic DNA. Translation: DAA07137.1.
PIRiS45870.
RefSeqiNP_009571.1. NM_001178363.1.

Genome annotation databases

EnsemblFungiiYBR015C; YBR015C; YBR015C.
GeneIDi852303.
KEGGisce:YBR015C.

Similar proteinsi

Entry informationi

Entry nameiMNN2_YEAST
AccessioniPrimary (citable) accession number: P38069
Secondary accession number(s): D6VQ17
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: September 27, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names