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Protein

GTP cyclohydrolase-2

Gene

RIB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.By similarity

Miscellaneous

Present with 9520 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. GTP cyclohydrolase-2 (RIB1)
  2. Bifunctional protein RIB2 (RIB2)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi148Zinc; catalyticBy similarity1
Metal bindingi159Zinc; catalyticBy similarity1
Metal bindingi161Zinc; catalyticBy similarity1
Binding sitei164GTPBy similarity1
Binding sitei219GTPBy similarity1
Active sitei231Proton acceptorSequence analysis1
Active sitei233NucleophileBy similarity1
Binding sitei254GTPBy similarity1
Binding sitei259GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi143 – 147GTPBy similarity5
Nucleotide bindingi197 – 199GTPBy similarity3

GO - Molecular functioni

  • cyclohydrolase activity Source: SGD
  • GTP binding Source: UniProtKB-KW
  • GTP cyclohydrolase II activity Source: UniProtKB-EC
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • riboflavin biosynthetic process Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processRiboflavin biosynthesis
LigandGTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YBL033C-MONOMER
YEAST:YBL033C-MONOMER
UniPathwayiUPA00275; UER00400

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase-2 (EC:3.5.4.25)
Alternative name(s):
GTP cyclohydrolase II
Gene namesi
Name:RIB1
Ordered Locus Names:YBL033C
ORF Names:YBL0417
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL033C
SGDiS000000129 RIB1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001517861 – 345GTP cyclohydrolase-2Add BLAST345

Proteomic databases

MaxQBiP38066
PaxDbiP38066
PRIDEiP38066

PTM databases

iPTMnetiP38066

Interactioni

Protein-protein interaction databases

BioGridi32664, 11 interactors
DIPiDIP-3936N
IntActiP38066, 12 interactors
MINTiP38066
STRINGi4932.YBL033C

Structurei

3D structure databases

ProteinModelPortaliP38066
SMRiP38066
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GTP cyclohydrolase II family.Curated

Phylogenomic databases

HOGENOMiHOG000115443
InParanoidiP38066
KOiK01497
OMAiIEKMGHM
OrthoDBiEOG092C1VZQ

Family and domain databases

CDDicd00641 GTP_cyclohydro2, 1 hit
Gene3Di3.40.50.10990, 1 hit
InterProiView protein in InterPro
IPR032677 GTP_cyclohydro_II
IPR000926 RibA
IPR036144 RibA-like_sf
PfamiView protein in Pfam
PF00925 GTP_cyclohydro2, 1 hit
SUPFAMiSSF142695 SSF142695, 2 hits
TIGRFAMsiTIGR00505 ribA, 1 hit

Sequencei

Sequence statusi: Complete.

P38066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIDNYDNSK QDSSKYEVSG TGDGRNGDGG LPLVQCVARA RIPTTQGPDI
60 70 80 90 100
FLHLYSNNRD NKEHLAIVFG EDIRSRSLFR RRQCETQQDR MIRGAYIGKL
110 120 130 140 150
YPGRTVADED DRLGLALEFD DSTGELLASK ATTWDAHNDT LVRIHSECYT
160 170 180 190 200
GENAWSARCD CGEQFDRAGR LIACDHEPTS NIKGGNGHGV IVYLRQEGRG
210 220 230 240 250
IGLGEKLKAY NLQDLGADTV QANLMLKHPV DARDFSLGKA ILLDLGIGNV
260 270 280 290 300
RLLTNNPEKI KQVDHAPYLK CVERVPMVPI HWTNSSEGID SKEIEGYLRT
310 320 330 340
KIERMGHLLT EPLKLHTNPQ PTETSEAQNQ NRMNSALSST STLAI
Length:345
Mass (Da):38,332
Last modified:December 15, 1998 - v2
Checksum:iD98D561CEB21A7D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35794 Genomic DNA Translation: CAA84853.1
X74738 Genomic DNA Translation: CAA52759.1
Z21617 Genomic DNA Translation: CAA79741.1
BK006936 Genomic DNA Translation: DAA07086.1
PIRiS45767
RefSeqiNP_009520.1, NM_001178273.1

Genome annotation databases

EnsemblFungiiYBL033C; YBL033C; YBL033C
GeneIDi852247
KEGGisce:YBL033C

Similar proteinsi

Entry informationi

Entry nameiRIB1_YEAST
AccessioniPrimary (citable) accession number: P38066
Secondary accession number(s): D6VPW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 15, 1998
Last modified: March 28, 2018
This is version 153 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health