P38066 (RIB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP cyclohydrolase-2
EC=3.5.4.25

Alternative name(s):
GTP cyclohydrolase II

Gene names

Name:	RIB1
Ordered Locus Names:	YBL033C
ORF Names:	YBL0417

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity.
Catalytic activity	GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4.
Miscellaneous	Present with 9520 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the GTP cyclohydrolase II family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	GTP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	riboflavin biosynthetic process Inferred from mutant phenotype PubMed 4555411PubMed 5366000. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase II activity Inferred from electronic annotation. Source: EC cyclohydrolase activity Inferred from genetic interaction PubMed 4555411. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-7436,EBI-7436

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	GTP cyclohydrolase-2		PRO_0000151786
Regions
	Nucleotide binding	143 – 147	5	GTP By similarity
	Nucleotide binding	197 – 199	3	GTP By similarity
Sites
	Active site	231	1	Proton acceptor Potential
	Active site	233	1	Nucleophile By similarity
	Metal binding	148	1	Zinc; catalytic By similarity
	Metal binding	159	1	Zinc; catalytic By similarity
	Metal binding	161	1	Zinc; catalytic By similarity
	Binding site	164	1	GTP By similarity
	Binding site	219	1	GTP By similarity
	Binding site	254	1	GTP By similarity
	Binding site	259	1	GTP By similarity
Amino acid modifications
	Modified residue	285	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P38066 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: D98D561CEB21A7D1	FASTA	345	38,332
	10 20 30 40 50 60 MTIDNYDNSK QDSSKYEVSG TGDGRNGDGG LPLVQCVARA RIPTTQGPDI FLHLYSNNRD 70 80 90 100 110 120 NKEHLAIVFG EDIRSRSLFR RRQCETQQDR MIRGAYIGKL YPGRTVADED DRLGLALEFD 130 140 150 160 170 180 DSTGELLASK ATTWDAHNDT LVRIHSECYT GENAWSARCD CGEQFDRAGR LIACDHEPTS 190 200 210 220 230 240 NIKGGNGHGV IVYLRQEGRG IGLGEKLKAY NLQDLGADTV QANLMLKHPV DARDFSLGKA 250 260 270 280 290 300 ILLDLGIGNV RLLTNNPEKI KQVDHAPYLK CVERVPMVPI HWTNSSEGID SKEIEGYLRT 310 320 330 340 KIERMGHLLT EPLKLHTNPQ PTETSEAQNQ NRMNSALSST STLAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of an 8.8 kb segment on the left arm of chromosome II from Saccharomyces cerevisiae reveals four new open reading frames including homologs of animal DNA polymerase alpha-primases and bacterial GTP cyclohydrolase II." Skala J., van Dyck L., Purnelle B., Goffeau A. Yeast 10:S13-S24(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Skala J., van Dyck L., Purnelle B., Goffeau A. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 181.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-285, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z35794 Genomic DNA. Translation: CAA84853.1. X74738 Genomic DNA. Translation: CAA52759.1. Z21617 Genomic DNA. Translation: CAA79741.1. BK006936 Genomic DNA. Translation: DAA07086.1.
PIR	S45767.
RefSeq	NP_009520.1. NM_001178273.1.
3D structure databases
ProteinModelPortal	P38066.
SMR	P38066. Positions 136-278.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3936N.
IntAct	P38066. 6 interactions.
MINT	MINT-497765.
STRING	4932.YBL033C.
Proteomic databases
PaxDb	P38066.
PeptideAtlas	P38066.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL033C; YBL033C; YBL033C.
GeneID	852247.
KEGG	sce:YBL033C.
Organism-specific databases
CYGD	YBL033c.
SGD	S000000129. RIB1.
Phylogenomic databases
eggNOG	COG0807.
HOGENOM	HOG000115443.
KO	K01497.
OMA	RSYGLAT.
OrthoDB	EOG4RBTTG.
Enzyme and pathway databases
BioCyc	MetaCyc:YBL033C-MONOMER.
UniPathway	UPA00275; UER00400.
Gene expression databases
Genevestigator	P38066.
GermOnline	YBL033C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000926. GTP_CycHdrlaseII_RibA. [Graphical view]
Pfam	PF00925. GTP_cyclohydro2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00505. ribA. 1 hit.
ProtoNet	Search...
Other
NextBio	970811.

Entry information

Entry name

RIB1_YEAST

Accession

Primary (citable) accession number: P38066
Secondary accession number(s): D6VPW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	December 15, 1998
Last modified:	May 1, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program