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P38065

- AP2A_YEAST

UniProt

P38065 - AP2A_YEAST

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Protein

AP-2 complex subunit alpha

Gene

APL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Alpha adaptin is a subunit of the plasma membrane adaptor. Facilitates interaction between APL1 and APS2.1 Publication

GO - Molecular functioni

  1. protein transporter activity Source: InterPro

GO - Biological processi

  1. endocytosis Source: UniProtKB-KW
  2. intracellular protein transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Endocytosis, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-28939-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-2 complex subunit alpha
Alternative name(s):
Alpha-adaptin
Clathrin assembly protein complex 2 alpha large chain
Clathrin assembly protein large alpha chain
Gene namesi
Name:APL3
Ordered Locus Names:YBL037W
ORF Names:YBL0412
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBL037w.
SGDiS000000133. APL3.

Subcellular locationi

Cell membrane. Membranecoated pit; Peripheral membrane protein; Cytoplasmic side
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.

GO - Cellular componenti

  1. AP-2 adaptor complex Source: SGD
  2. cellular bud neck Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Coated pit, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10251025AP-2 complex subunit alphaPRO_0000193737Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei727 – 7271Phosphothreonine2 Publications
Modified residuei733 – 7331Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38065.
PaxDbiP38065.
PeptideAtlasiP38065.

Expressioni

Gene expression databases

GenevestigatoriP38065.

Interactioni

Subunit structurei

Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit APL3 and beta-type subunit APL1), a medium chain (mu-type subunit APM4) and a small adaptin (sigma-type subunit APS2).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
APS2Q003813EBI-2181,EBI-2608

Protein-protein interaction databases

BioGridi32660. 41 interactions.
DIPiDIP-2634N.
IntActiP38065. 27 interactions.
MINTiMINT-426340.
STRINGi4932.YBL037W.

Structurei

3D structure databases

ProteinModelPortaliP38065.
SMRiP38065. Positions 22-641, 753-992.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG236069.
GeneTreeiENSGT00550000074757.
HOGENOMiHOG000033976.
InParanoidiP38065.
KOiK11824.
OMAiFTIINDR.
OrthoDBiEOG7JHMDS.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1030. 1 hit.
3.30.310.30. 1 hit.
InterProiIPR017104. AP2_complex_asu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR013038. Clathrin_a-adaptin_app_Ig-like.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR015873. Clathrin_a/coatomer_app_sub_C.
IPR009028. Coatomer/calthrin_app_sub_C.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PfamiPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view]
PIRSFiPIRSF037091. AP2_complex_alpha. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
SSF55711. SSF55711. 1 hit.

Sequencei

Sequence statusi: Complete.

P38065-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDRKKTLINS SVSNNNSTIK GLQLFIADLR SAQQAQEQEK RIQSEIVKIK
60 70 80 90 100
QHFDAAKKKQ GNHDRLGGYQ RKKYVAKLAY IYITSNTTKL NEILFGLEQT
110 120 130 140 150
VELLKSSIFS EKFIGYMTLE LLYERSEVVA KVNDEVNYQL MKDLSSSDDN
160 170 180 190 200
FVMLALNFVG VVGELTNRLA YNDDITTGVF KILRSPTSSI YLKKKSALSF
210 220 230 240 250
LALLKSNHSI LTEDLQRKQL WIQRILSLLD DTENYRLTLA TIPLIEFIAK
260 270 280 290 300
YIDPSYCTRL LPQLTEILYN CVVVGTSRSS DNQFPLEYTF ANMPNPWLIT
310 320 330 340 350
KVVSLLSILI ASPTERDSGS LLQTNNIDNE LLNKLRKCVS VAIELGTRQA
360 370 380 390 400
QDPMERIVQN TVLFSLINFA SKLDPSDEAI SNSVTALCSL LTSKEINIRY
410 420 430 440 450
LTLDSLVKLC SSSGKPAIDA VRYKNLDMIF HLLNTERDSS IVRKVVDLLY
460 470 480 490 500
TFTDVENVKI IVDGLLQYIL SPKNLAEPQI KSDIAVKIAI LTEKYATDIN
510 520 530 540 550
WFVIISLQLL SLTSNTTIND DEIWQRLCQI VVNNPSLHRI TCERLVDYLC
560 570 580 590 600
KKQASEAIIK AAAFLLGEYS SLITDRISSA NLFTLFAEKY FSAPNVAKAM
610 620 630 640 650
ILTTMIKLYK TSPEIGSNVI KFFQLELNSL DIELQTRSFE YLNIIQLAKV
660 670 680 690 700
NGNTDILQIL FEPMPPFNSK SNPLLKRLGS LPASAGSTTL INTPSEASSS
710 720 730 740 750
TPDLLSKRAN SSRSIMVPMP PPSRRNTIDD VNSKISSSED FSGKDSYYSR
760 770 780 790 800
QILAPNWREG FTRMISHKQG VLFTSSLMKV FYRITTPDAQ QPYVFHISLA
810 820 830 840 850
FINLTEWEIT GLSTQIIPSK TQGNPEYLIM NINTPSTATI GPHKRAEQSY
860 870 880 890 900
EVSIRKPFDV EDSPILAIHF KCGGSTNTIN LKTAIGMTTT LISSDVNPSM
910 920 930 940 950
HLNLAQFISR WKTLSDALGK EGEYQKSGIK LNKDFRKVET ISLEDGLLLL
960 970 980 990 1000
TQTVKRLGFD IVDQTSVRST LFVSGIIHTK SEGNFGCLMK IQYQVNGTVN
1010 1020
VTCKTTTAGP LAKYIVECIK NVLTK
Length:1,025
Mass (Da):115,012
Last modified:October 1, 1994 - v1
Checksum:i629DF3C7C5AF88E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78214 Genomic DNA. Translation: CAA55057.1.
Z35798 Genomic DNA. Translation: CAA84857.1.
BK006936 Genomic DNA. Translation: DAA07082.1.
PIRiS50293.
RefSeqiNP_009516.1. NM_001178277.1.

Genome annotation databases

EnsemblFungiiYBL037W; YBL037W; YBL037W.
GeneIDi852243.
KEGGisce:YBL037W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78214 Genomic DNA. Translation: CAA55057.1 .
Z35798 Genomic DNA. Translation: CAA84857.1 .
BK006936 Genomic DNA. Translation: DAA07082.1 .
PIRi S50293.
RefSeqi NP_009516.1. NM_001178277.1.

3D structure databases

ProteinModelPortali P38065.
SMRi P38065. Positions 22-641, 753-992.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 32660. 41 interactions.
DIPi DIP-2634N.
IntActi P38065. 27 interactions.
MINTi MINT-426340.
STRINGi 4932.YBL037W.

Proteomic databases

MaxQBi P38065.
PaxDbi P38065.
PeptideAtlasi P38065.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YBL037W ; YBL037W ; YBL037W .
GeneIDi 852243.
KEGGi sce:YBL037W.

Organism-specific databases

CYGDi YBL037w.
SGDi S000000133. APL3.

Phylogenomic databases

eggNOGi NOG236069.
GeneTreei ENSGT00550000074757.
HOGENOMi HOG000033976.
InParanoidi P38065.
KOi K11824.
OMAi FTIINDR.
OrthoDBi EOG7JHMDS.

Enzyme and pathway databases

BioCyci YEAST:G3O-28939-MONOMER.

Miscellaneous databases

NextBioi 970799.
PROi P38065.

Gene expression databases

Genevestigatori P38065.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
2.60.40.1030. 1 hit.
3.30.310.30. 1 hit.
InterProi IPR017104. AP2_complex_asu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR013038. Clathrin_a-adaptin_app_Ig-like.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR015873. Clathrin_a/coatomer_app_sub_C.
IPR009028. Coatomer/calthrin_app_sub_C.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view ]
Pfami PF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view ]
PIRSFi PIRSF037091. AP2_complex_alpha. 1 hit.
SUPFAMi SSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
SSF55711. SSF55711. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 22.4 kb DNA fragment from the left arm of yeast chromosome II reveals homologues to bacterial proline synthetase and murine alpha-adaptin, as well as a new permease and a DNA-binding protein."
    de Wergifosse P., Jacques B., Jonniaux J.-L., Purnelle B., Skala J., Goffeau A.
    Yeast 10:1489-1496(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Adaptor complex-independent clathrin function in yeast."
    Yeung B.G., Phan H.L., Payne G.S.
    Mol. Biol. Cell 10:3643-3659(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-727, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-727 AND SER-733, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAP2A_YEAST
AccessioniPrimary (citable) accession number: P38065
Secondary accession number(s): D6VPW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: October 29, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1590 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3