P38065 (AP2A_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-2 complex subunit alpha

Alternative name(s):
Alpha-adaptin
Clathrin assembly protein complex 2 alpha large chain
Clathrin assembly protein large alpha chain

Gene names

Name:	APL3
Ordered Locus Names:	YBL037W
ORF Names:	YBL0412

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1025 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Alpha adaptin is a subunit of the plasma membrane adaptor. Facilitates interaction between APL1 and APS2. Ref.4
Subunit structure	Assembly protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit APL3 and beta-type subunit APL1), a medium chain (mu-type subunit APM4) and a small adaptin (sigma-type subunit APS2). Ref.4
Subcellular location	Cell membrane. Membrane › coated pit; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.
Miscellaneous	Present with 1590 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the adaptor complexes large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Coated pit Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred by curator Ref.4. Source: SGD vesicle-mediated transport Inferred from electronic annotation. Source: InterPro
Cellular_component	AP-2 adaptor complex Inferred from mutant phenotype Ref.4. Source: SGD cellular bud neck Inferred from direct assay PubMed 19458192. Source: SGD
Molecular_function	protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APS2	Q00381	3	EBI-2181,EBI-2608

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1025	1025	AP-2 complex subunit alpha		PRO_0000193737
Amino acid modifications
	Modified residue	320	1	Phosphoserine Ref.7
	Modified residue	698	1	Phosphoserine Ref.6 Ref.7
	Modified residue	700	1	Phosphoserine Ref.6 Ref.7
	Modified residue	701	1	Phosphothreonine Ref.7
	Modified residue	723	1	Phosphoserine Ref.7
	Modified residue	727	1	Phosphothreonine Ref.6 Ref.7
	Modified residue	736	1	Phosphoserine Ref.7
	Modified residue	737	1	Phosphoserine Ref.7
	Modified residue	738	1	Phosphoserine Ref.6 Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P38065 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 629DF3C7C5AF88E5	FASTA	1,025	115,012
	10 20 30 40 50 60 MDRKKTLINS SVSNNNSTIK GLQLFIADLR SAQQAQEQEK RIQSEIVKIK QHFDAAKKKQ 70 80 90 100 110 120 GNHDRLGGYQ RKKYVAKLAY IYITSNTTKL NEILFGLEQT VELLKSSIFS EKFIGYMTLE 130 140 150 160 170 180 LLYERSEVVA KVNDEVNYQL MKDLSSSDDN FVMLALNFVG VVGELTNRLA YNDDITTGVF 190 200 210 220 230 240 KILRSPTSSI YLKKKSALSF LALLKSNHSI LTEDLQRKQL WIQRILSLLD DTENYRLTLA 250 260 270 280 290 300 TIPLIEFIAK YIDPSYCTRL LPQLTEILYN CVVVGTSRSS DNQFPLEYTF ANMPNPWLIT 310 320 330 340 350 360 KVVSLLSILI ASPTERDSGS LLQTNNIDNE LLNKLRKCVS VAIELGTRQA QDPMERIVQN 370 380 390 400 410 420 TVLFSLINFA SKLDPSDEAI SNSVTALCSL LTSKEINIRY LTLDSLVKLC SSSGKPAIDA 430 440 450 460 470 480 VRYKNLDMIF HLLNTERDSS IVRKVVDLLY TFTDVENVKI IVDGLLQYIL SPKNLAEPQI 490 500 510 520 530 540 KSDIAVKIAI LTEKYATDIN WFVIISLQLL SLTSNTTIND DEIWQRLCQI VVNNPSLHRI 550 560 570 580 590 600 TCERLVDYLC KKQASEAIIK AAAFLLGEYS SLITDRISSA NLFTLFAEKY FSAPNVAKAM 610 620 630 640 650 660 ILTTMIKLYK TSPEIGSNVI KFFQLELNSL DIELQTRSFE YLNIIQLAKV NGNTDILQIL 670 680 690 700 710 720 FEPMPPFNSK SNPLLKRLGS LPASAGSTTL INTPSEASSS TPDLLSKRAN SSRSIMVPMP 730 740 750 760 770 780 PPSRRNTIDD VNSKISSSED FSGKDSYYSR QILAPNWREG FTRMISHKQG VLFTSSLMKV 790 800 810 820 830 840 FYRITTPDAQ QPYVFHISLA FINLTEWEIT GLSTQIIPSK TQGNPEYLIM NINTPSTATI 850 860 870 880 890 900 GPHKRAEQSY EVSIRKPFDV EDSPILAIHF KCGGSTNTIN LKTAIGMTTT LISSDVNPSM 910 920 930 940 950 960 HLNLAQFISR WKTLSDALGK EGEYQKSGIK LNKDFRKVET ISLEDGLLLL TQTVKRLGFD 970 980 990 1000 1010 1020 IVDQTSVRST LFVSGIIHTK SEGNFGCLMK IQYQVNGTVN VTCKTTTAGP LAKYIVECIK NVLTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 22.4 kb DNA fragment from the left arm of yeast chromosome II reveals homologues to bacterial proline synthetase and murine alpha-adaptin, as well as a new permease and a DNA-binding protein." de Wergifosse P., Jacques B., Jonniaux J.-L., Purnelle B., Skala J., Goffeau A. Yeast 10:1489-1496(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Adaptor complex-independent clathrin function in yeast." Yeung B.G., Phan H.L., Payne G.S. Mol. Biol. Cell 10:3643-3659(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-698; SER-700; THR-727 AND SER-738, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-320; SER-698; SER-700; THR-701; SER-723; THR-727; SER-736; SER-737 AND SER-738, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78214 Genomic DNA. Translation: CAA55057.1. Z35798 Genomic DNA. Translation: CAA84857.1. BK006936 Genomic DNA. Translation: DAA07082.1.
PIR	S50293.
RefSeq	NP_009516.1. NM_001178277.1.
3D structure databases
ProteinModelPortal	P38065.
SMR	P38065. Positions 22-641, 753-992.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2634N.
IntAct	P38065. 25 interactions.
MINT	MINT-426340.
STRING	4932.YBL037W.
Proteomic databases
PaxDb	P38065.
PeptideAtlas	P38065.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL037W; YBL037W; YBL037W.
GeneID	852243.
KEGG	sce:YBL037W.
Organism-specific databases
CYGD	YBL037w.
SGD	S000000133. APL3.
Phylogenomic databases
eggNOG	NOG236069.
GeneTree	ENSGT00550000074757.
HOGENOM	HOG000033976.
KO	K11824.
OMA	CQIVVNN.
OrthoDB	EOG4TB7KH.
Gene expression databases
Genevestigator	P38065.
GermOnline	YBL037W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.10.10. 1 hit. 2.60.40.1030. 1 hit. 3.30.310.30. 1 hit.
InterPro	IPR017104. AP2_complex_asu. IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR002553. Clathrin/coatomer_adapt-like_N. IPR013038. Clathrin_a-adaptin_app_Ig-like. IPR008152. Clathrin_a/b/g-adaptin_app_Ig. IPR015873. Clathrin_a/coatomer_app_sub_C. IPR009028. Coatomer/calthrin_app_sub_C. IPR013041. Coatomer/clathrin_app_Ig-like. [Graphical view]
PANTHER	PTHR22780:SF3. PTHR22780:SF3. 1 hit.
Pfam	PF01602. Adaptin_N. 1 hit. PF02883. Alpha_adaptinC2. 1 hit. [Graphical view]
PIRSF	PIRSF037091. AP2_complex_alpha. 1 hit.
SUPFAM	SSF55711. AP2_adap_app. 1 hit. SSF48371. ARM-type_fold. 1 hit. SSF49348. Clath_adapt. 1 hit.
ProtoNet	Search...
Other
NextBio	970799.

Entry information

Entry name

AP2A_YEAST

Accession

Primary (citable) accession number: P38065
Secondary accession number(s): D6VPW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program