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P38065

- AP2A_YEAST

UniProt

P38065 - AP2A_YEAST

Protein

AP-2 complex subunit alpha

Gene

APL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Alpha adaptin is a subunit of the plasma membrane adaptor. Facilitates interaction between APL1 and APS2.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein transporter activity Source: InterPro

    GO - Biological processi

    1. endocytosis Source: UniProtKB-KW
    2. intracellular protein transport Source: SGD

    Keywords - Biological processi

    Endocytosis, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28939-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-2 complex subunit alpha
    Alternative name(s):
    Alpha-adaptin
    Clathrin assembly protein complex 2 alpha large chain
    Clathrin assembly protein large alpha chain
    Gene namesi
    Name:APL3
    Ordered Locus Names:YBL037W
    ORF Names:YBL0412
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL037w.
    SGDiS000000133. APL3.

    Subcellular locationi

    Cell membrane. Membranecoated pit; Peripheral membrane protein; Cytoplasmic side
    Note: Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.

    GO - Cellular componenti

    1. AP-2 adaptor complex Source: SGD
    2. cellular bud neck Source: SGD

    Keywords - Cellular componenti

    Cell membrane, Coated pit, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10251025AP-2 complex subunit alphaPRO_0000193737Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei727 – 7271Phosphothreonine2 Publications
    Modified residuei733 – 7331Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38065.
    PaxDbiP38065.
    PeptideAtlasiP38065.

    Expressioni

    Gene expression databases

    GenevestigatoriP38065.

    Interactioni

    Subunit structurei

    Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit APL3 and beta-type subunit APL1), a medium chain (mu-type subunit APM4) and a small adaptin (sigma-type subunit APS2).1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    APS2Q003813EBI-2181,EBI-2608

    Protein-protein interaction databases

    BioGridi32660. 40 interactions.
    DIPiDIP-2634N.
    IntActiP38065. 27 interactions.
    MINTiMINT-426340.
    STRINGi4932.YBL037W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38065.
    SMRiP38065. Positions 22-641, 753-992.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG236069.
    GeneTreeiENSGT00550000074757.
    HOGENOMiHOG000033976.
    KOiK11824.
    OMAiFTIINDR.
    OrthoDBiEOG7JHMDS.

    Family and domain databases

    Gene3Di1.25.10.10. 1 hit.
    2.60.40.1030. 1 hit.
    3.30.310.30. 1 hit.
    InterProiIPR017104. AP2_complex_asu.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR002553. Clathrin/coatomer_adapt-like_N.
    IPR013038. Clathrin_a-adaptin_app_Ig-like.
    IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
    IPR015873. Clathrin_a/coatomer_app_sub_C.
    IPR009028. Coatomer/calthrin_app_sub_C.
    IPR013041. Coatomer/clathrin_app_Ig-like.
    [Graphical view]
    PfamiPF01602. Adaptin_N. 1 hit.
    PF02883. Alpha_adaptinC2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037091. AP2_complex_alpha. 1 hit.
    SUPFAMiSSF48371. SSF48371. 1 hit.
    SSF49348. SSF49348. 1 hit.
    SSF55711. SSF55711. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38065-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDRKKTLINS SVSNNNSTIK GLQLFIADLR SAQQAQEQEK RIQSEIVKIK     50
    QHFDAAKKKQ GNHDRLGGYQ RKKYVAKLAY IYITSNTTKL NEILFGLEQT 100
    VELLKSSIFS EKFIGYMTLE LLYERSEVVA KVNDEVNYQL MKDLSSSDDN 150
    FVMLALNFVG VVGELTNRLA YNDDITTGVF KILRSPTSSI YLKKKSALSF 200
    LALLKSNHSI LTEDLQRKQL WIQRILSLLD DTENYRLTLA TIPLIEFIAK 250
    YIDPSYCTRL LPQLTEILYN CVVVGTSRSS DNQFPLEYTF ANMPNPWLIT 300
    KVVSLLSILI ASPTERDSGS LLQTNNIDNE LLNKLRKCVS VAIELGTRQA 350
    QDPMERIVQN TVLFSLINFA SKLDPSDEAI SNSVTALCSL LTSKEINIRY 400
    LTLDSLVKLC SSSGKPAIDA VRYKNLDMIF HLLNTERDSS IVRKVVDLLY 450
    TFTDVENVKI IVDGLLQYIL SPKNLAEPQI KSDIAVKIAI LTEKYATDIN 500
    WFVIISLQLL SLTSNTTIND DEIWQRLCQI VVNNPSLHRI TCERLVDYLC 550
    KKQASEAIIK AAAFLLGEYS SLITDRISSA NLFTLFAEKY FSAPNVAKAM 600
    ILTTMIKLYK TSPEIGSNVI KFFQLELNSL DIELQTRSFE YLNIIQLAKV 650
    NGNTDILQIL FEPMPPFNSK SNPLLKRLGS LPASAGSTTL INTPSEASSS 700
    TPDLLSKRAN SSRSIMVPMP PPSRRNTIDD VNSKISSSED FSGKDSYYSR 750
    QILAPNWREG FTRMISHKQG VLFTSSLMKV FYRITTPDAQ QPYVFHISLA 800
    FINLTEWEIT GLSTQIIPSK TQGNPEYLIM NINTPSTATI GPHKRAEQSY 850
    EVSIRKPFDV EDSPILAIHF KCGGSTNTIN LKTAIGMTTT LISSDVNPSM 900
    HLNLAQFISR WKTLSDALGK EGEYQKSGIK LNKDFRKVET ISLEDGLLLL 950
    TQTVKRLGFD IVDQTSVRST LFVSGIIHTK SEGNFGCLMK IQYQVNGTVN 1000
    VTCKTTTAGP LAKYIVECIK NVLTK 1025
    Length:1,025
    Mass (Da):115,012
    Last modified:October 1, 1994 - v1
    Checksum:i629DF3C7C5AF88E5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78214 Genomic DNA. Translation: CAA55057.1.
    Z35798 Genomic DNA. Translation: CAA84857.1.
    BK006936 Genomic DNA. Translation: DAA07082.1.
    PIRiS50293.
    RefSeqiNP_009516.1. NM_001178277.1.

    Genome annotation databases

    EnsemblFungiiYBL037W; YBL037W; YBL037W.
    GeneIDi852243.
    KEGGisce:YBL037W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78214 Genomic DNA. Translation: CAA55057.1 .
    Z35798 Genomic DNA. Translation: CAA84857.1 .
    BK006936 Genomic DNA. Translation: DAA07082.1 .
    PIRi S50293.
    RefSeqi NP_009516.1. NM_001178277.1.

    3D structure databases

    ProteinModelPortali P38065.
    SMRi P38065. Positions 22-641, 753-992.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32660. 40 interactions.
    DIPi DIP-2634N.
    IntActi P38065. 27 interactions.
    MINTi MINT-426340.
    STRINGi 4932.YBL037W.

    Proteomic databases

    MaxQBi P38065.
    PaxDbi P38065.
    PeptideAtlasi P38065.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL037W ; YBL037W ; YBL037W .
    GeneIDi 852243.
    KEGGi sce:YBL037W.

    Organism-specific databases

    CYGDi YBL037w.
    SGDi S000000133. APL3.

    Phylogenomic databases

    eggNOGi NOG236069.
    GeneTreei ENSGT00550000074757.
    HOGENOMi HOG000033976.
    KOi K11824.
    OMAi FTIINDR.
    OrthoDBi EOG7JHMDS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28939-MONOMER.

    Miscellaneous databases

    NextBioi 970799.
    PROi P38065.

    Gene expression databases

    Genevestigatori P38065.

    Family and domain databases

    Gene3Di 1.25.10.10. 1 hit.
    2.60.40.1030. 1 hit.
    3.30.310.30. 1 hit.
    InterProi IPR017104. AP2_complex_asu.
    IPR011989. ARM-like.
    IPR016024. ARM-type_fold.
    IPR002553. Clathrin/coatomer_adapt-like_N.
    IPR013038. Clathrin_a-adaptin_app_Ig-like.
    IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
    IPR015873. Clathrin_a/coatomer_app_sub_C.
    IPR009028. Coatomer/calthrin_app_sub_C.
    IPR013041. Coatomer/clathrin_app_Ig-like.
    [Graphical view ]
    Pfami PF01602. Adaptin_N. 1 hit.
    PF02883. Alpha_adaptinC2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037091. AP2_complex_alpha. 1 hit.
    SUPFAMi SSF48371. SSF48371. 1 hit.
    SSF49348. SSF49348. 1 hit.
    SSF55711. SSF55711. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of a 22.4 kb DNA fragment from the left arm of yeast chromosome II reveals homologues to bacterial proline synthetase and murine alpha-adaptin, as well as a new permease and a DNA-binding protein."
      de Wergifosse P., Jacques B., Jonniaux J.-L., Purnelle B., Skala J., Goffeau A.
      Yeast 10:1489-1496(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Adaptor complex-independent clathrin function in yeast."
      Yeung B.G., Phan H.L., Payne G.S.
      Mol. Biol. Cell 10:3643-3659(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-727, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-727 AND SER-733, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiAP2A_YEAST
    AccessioniPrimary (citable) accession number: P38065
    Secondary accession number(s): D6VPW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1590 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3