P38064 (RM16_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
54S ribosomal protein L16, mitochondrial

Alternative name(s):
YmL47

Gene names

Name:	MRPL16
Synonyms:	RML16
Ordered Locus Names:	YBL038W
ORF Names:	YBL0411

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.5 Ref.6
Subcellular location	Mitochondrion Ref.7 Ref.9.
Miscellaneous	Present with 2900 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the ribosomal protein L16P family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial translation Inferred by curator Ref.5. Source: SGD
Cellular component	mitochondrial large ribosomal subunit Inferred from direct assay Ref.5. Source: SGD
Molecular function	rRNA binding Inferred from electronic annotation. Source: InterPro structural constituent of ribosome Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 41	41	Mitochondrion Potential
	Chain	42 – 232	191	54S ribosomal protein L16, mitochondrial		PRO_0000030469
Experimental info
	Sequence conflict	55	1	R → Q in CAA37942. Ref.1
	Sequence conflict	119	1	V → L in CAA37942. Ref.1
	Sequence conflict	209	1	K → N in CAA37942. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38064 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 6E0C11EADC4C156C	FASTA	232	26,518
	10 20 30 40 50 60 MFPYLTRMNL SIKMGGLTLK ESSPNAFLNN TTIARRFKHE YAPRFKIVQK KQKGRVPVRT 70 80 90 100 110 120 GGSIKGSTLQ FGKYGLRLKS EGIRISAQQL KEADNAIMRY VRPLNNGHLW RRLCTNVAVC 130 140 150 160 170 180 IKGNETRMGK GKGGFDHWMV RVPTGKILFE INGDDLHEKV AREAFRKAGT KLPGVYEFVS 190 200 210 220 230 LDSLVRVGLH SFKNPKDDPV KNFYDENAKK PSKKYLNILK SQEPQYKLFR GR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Ozier-Kalogeropoulos O. Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of a 22.4 kb DNA fragment from the left arm of yeast chromosome II reveals homologues to bacterial proline synthetase and murine alpha-adaptin, as well as a new permease and a DNA-binding protein." de Wergifosse P., Jacques B., Jonniaux J.-L., Purnelle B., Skala J., Goffeau A. Yeast 10:1489-1496(1994) [PubMed: 7871888] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Identification and characterization of the genes for mitochondrial ribosomal proteins of Saccharomyces cerevisiae." Kitakawa M., Graack H.-R., Grohmann L., Goldschmidt-Reisin S., Herfurth E., Wittmann-Liebold B., Nishimura T., Isono K. Eur. J. Biochem. 245:449-456(1997) [PubMed: 9151978] [Abstract] Cited for: PROTEIN SEQUENCE OF 202-209, SUBUNIT. Strain: 07173.
[6]	"Identification of the yeast nuclear gene for the mitochondrial homologue of bacterial ribosomal protein L16." Pan C., Mason T.L. Nucleic Acids Res. 23:3673-3677(1995) [PubMed: 7478995] [Abstract] Cited for: FUNCTION, SUBUNIT.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53995 Genomic DNA. Translation: CAA37942.1. X78214 Genomic DNA. Translation: CAA55056.1. Z35799 Genomic DNA. Translation: CAA84858.1. BK006936 Genomic DNA. Translation: DAA07081.1.
PIR	S50292.
RefSeq	NP_009515.1. NM_001178278.1.
3D structure databases
ProteinModelPortal	P38064.
SMR	P38064. Positions 40-183.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3939N.
IntAct	P38064. 33 interactions.
MINT	MINT-535690.
STRING	P38064.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL038W; YBL038W; YBL038W.
GeneID	852242.
KEGG	sce:YBL038W.
NMPDR	fig|4932.3.peg.207.
Organism-specific databases
CYGD	YBL038w.
SGD	S000000134. MRPL16.
Phylogenomic databases
eggNOG	fuNOG07991.
GeneTree	EFGT00050000006773.
HOGENOM	HBG286097.
OMA	HEYAPRF.
OrthoDB	EOG4T4H51.
Gene expression databases
ArrayExpress	P38064.
Genevestigator	P38064.
GermOnline	YBL038W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016180. Ribosomal_L10e/L16. IPR000114. Ribosomal_L16. IPR020798. Ribosomal_L16_CS. [Graphical view]
PANTHER	PTHR12220. Ribosomal_L16. 1 hit.
Pfam	PF00252. Ribosomal_L16. 1 hit. [Graphical view]
PRINTS	PR00060. RIBOSOMALL16.
SUPFAM	SSF54686. Ribosomal_L10e/L16. 1 hit.
TIGRFAMs	TIGR01164. RplP_bact. 1 hit.
PROSITE	PS00586. RIBOSOMAL_L16_1. 1 hit. PS00701. RIBOSOMAL_L16_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970796.

Entry information

Entry name

RM16_YEAST

Accession

Primary (citable) accession number: P38064
Secondary accession number(s): D6VPW1, Q07216

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program