Reviewed,
UniProtKB/Swiss-Prot P38063 (KPR4_YEAST)
Last modified
February 9, 2010.
Version 77.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Ribose-phosphate pyrophosphokinase 4 EC=2.7.6.1 Alternative name(s): Phosphoribosyl pyrophosphate synthetase 4 | ||||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 327 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | 5-phosphoribose 1-diphosphate synthetase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1-diphosphate synthetases (PRS2, PRS3, PRS4 and PRS5). Ref.1 Ref.3 |
| Catalytic activity | ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. Ref.1 Ref.3 |
| Pathway | |
| Subcellular location | |
| Miscellaneous | Present with 5280 molecules/cell in log phase SD medium. Ref.6 |
| Sequence similarities | Belongs to the ribose-phosphate pyrophosphokinase family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| PRS1 | P32895 | 1 | EBI-9882,EBI-9869 | |
| PRS2 | P38620 | 1 | EBI-9882,EBI-9873 | |
| PRS5 | Q12265 | 1 | EBI-9882,EBI-9886 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 327 | 327 | Ribose-phosphate pyrophosphokinase 4 | PRO_0000141089 | |||||
Sites | |||||||||
| Metal binding | 140 | 1 | Magnesium Potential | ||||||
| Metal binding | 142 | 1 | Magnesium Potential | ||||||
| Metal binding | 151 | 1 | Magnesium Potential | ||||||
| Metal binding | 155 | 1 | Magnesium Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 90 | 1 | Q → K in CAC04170. Ref.1 | ||||||
| Sequence conflict | 169 – 170 | 2 | AR → T in CAC04170. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Heterooligomeric phosphoribosyl diphosphate synthase of Saccharomyces cerevisiae: combinatorial expression of the five PRS genes in Escherichia coli." Hove-Jensen B. J. Biol. Chem. 279:40345-40350(2004) [PubMed: 15280369] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ENZYME ACTIVITY. |
| [2] | "Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K.EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "Genetic analysis and enzyme activity suggest the existence of more than one minimal functional unit capable of synthesizing phosphoribosyl pyrophosphate in Saccharomyces cerevisiae." Hernando Y., Carter A.T., Parr A., Hove-Jensen B., Schweizer M. J. Biol. Chem. 274:12480-12487(1999) [PubMed: 10212224] [Abstract] Cited for: FUNCTION, ENZYME ACTIVITY. |
| [4] | "Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed: 12748633] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE. |
| [5] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [6] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AJ245726 Genomic DNA. Translation: CAC04170.1. Different initiation. Z35829 Genomic DNA. Translation: CAA84888.1. Different initiation. |
| PIR | S45804. |
| RefSeq | NP_009485.2. |
3D structure databases | |
| SMR | P38063. Positions 13-326. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-3945N. |
| IntAct | P38063. 4 interactions. |
| STRING | P38063. |
Proteomic databases | |
| PeptideAtlas | P38063. |
| PRIDE | P38063. |
Genome annotation databases | |
| Ensembl | YBL068W; YBL068W; YBL068W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 852211. |
| KEGG | sce:YBL068W. |
| NMPDR | fig|4932.3.peg.174. |
Organism-specific databases | |
| CYGD | YBL068w. |
| SGD | S000000164. PRS4. |
Phylogenomic databases | |
| eggNOG | fuNOG04549. |
| HOGENOM | HBG519284. |
| OMA | TGGTMAT. |
| OrthoDB | EOG9JDJPX. |
| PhylomeDB | P38063. |
Enzyme and pathway databases | |
| BRENDA | 2.7.6.1. 250. |
Gene expression databases | |
| ArrayExpress | P38063. |
| Genevestigator | P38063. |
| GermOnline | YBL068W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view] |
| Pfam | PF00156. Pribosyltran. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01251. ribP_PPkin. 1 hit. |
| PROSITE | PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 970715. |
Entry information
| Entry name | KPR4_YEAST | ||||||||
| Accession | Primary (citable) accession number: P38063 Secondary accession number(s): Q9HGQ6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome II Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names |

Clusters with


