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P38061

- RL32_YEAST

UniProt

P38061 - RL32_YEAST

Protein

60S ribosomal protein L32

Gene

RPL32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28980-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    60S ribosomal protein L32
    Gene namesi
    Name:RPL32
    Ordered Locus Names:YBL092W
    ORF Names:YBL0838
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL092w.
    SGDiS000000188. RPL32.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosolic large ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 13012960S ribosomal protein L32PRO_0000131145Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei40 – 401Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38061.
    PaxDbiP38061.
    PeptideAtlasiP38061.

    Expressioni

    Gene expression databases

    GenevestigatoriP38061.

    Interactioni

    Subunit structurei

    Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi32611. 70 interactions.
    DIPiDIP-5176N.
    IntActiP38061. 7 interactions.
    MINTiMINT-8285132.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Ymodel-X14-122[»]
    1S1Ielectron microscopy11.7001-130[»]
    1VW8electron microscopy6.10f1-130[»]
    1VWUelectron microscopy6.10f1-130[»]
    3IZSelectron microscopy-h1-130[»]
    3J65electron microscopy8.70d1-130[»]
    3O58X-ray4.00b1-130[»]
    3O5HX-ray4.00b1-130[»]
    3U5EX-ray3.00e1-130[»]
    3U5IX-ray3.00e1-130[»]
    4B6Aelectron microscopy8.10e1-130[»]
    4BYNelectron microscopy4.30e2-130[»]
    4BYUelectron microscopy6.60e2-130[»]
    4CUWelectron microscopy3.70e1-130[»]
    ProteinModelPortaliP38061.
    SMRiP38061. Positions 2-128.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP38061.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L32e family.Curated

    Phylogenomic databases

    eggNOGiCOG1717.
    GeneTreeiENSGT00390000014729.
    HOGENOMiHOG000231288.
    KOiK02912.
    OMAiTRHMLPT.
    OrthoDBiEOG7H79FB.

    Family and domain databases

    InterProiIPR001515. Ribosomal_L32e.
    IPR018263. Ribosomal_L32e_CS.
    [Graphical view]
    PfamiPF01655. Ribosomal_L32e. 1 hit.
    [Graphical view]
    ProDomiPD003823. Ribosomal_L32e. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF52042. SSF52042. 1 hit.
    PROSITEiPS00580. RIBOSOMAL_L32E. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P38061-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASLPHPKIV KKHTKKFKRH HSDRYHRVAE NWRKQKGIDS VVRRRFRGNI    50
    SQPKIGYGSN KKTKFLSPSG HKTFLVANVK DLETLTMHTK TYAAEIAHNI 100
    SAKNRVVILA RAKALGIKVT NPKGRLALEA 130
    Length:130
    Mass (Da):14,771
    Last modified:October 1, 1994 - v1
    Checksum:i5C5EDD87D6841976
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79489 Genomic DNA. Translation: CAA56010.1.
    Z35853 Genomic DNA. Translation: CAA84914.1.
    AY558291 Genomic DNA. Translation: AAS56617.1.
    EF123124 mRNA. Translation: ABM97468.1.
    BK006936 Genomic DNA. Translation: DAA07031.1.
    PIRiS45410.
    RefSeqiNP_009460.1. NM_001178332.1.

    Genome annotation databases

    EnsemblFungiiYBL092W; YBL092W; YBL092W.
    GeneIDi852185.
    KEGGisce:YBL092W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79489 Genomic DNA. Translation: CAA56010.1 .
    Z35853 Genomic DNA. Translation: CAA84914.1 .
    AY558291 Genomic DNA. Translation: AAS56617.1 .
    EF123124 mRNA. Translation: ABM97468.1 .
    BK006936 Genomic DNA. Translation: DAA07031.1 .
    PIRi S45410.
    RefSeqi NP_009460.1. NM_001178332.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5Y model - X 14-122 [» ]
    1S1I electron microscopy 11.70 0 1-130 [» ]
    1VW8 electron microscopy 6.10 f 1-130 [» ]
    1VWU electron microscopy 6.10 f 1-130 [» ]
    3IZS electron microscopy - h 1-130 [» ]
    3J65 electron microscopy 8.70 d 1-130 [» ]
    3O58 X-ray 4.00 b 1-130 [» ]
    3O5H X-ray 4.00 b 1-130 [» ]
    3U5E X-ray 3.00 e 1-130 [» ]
    3U5I X-ray 3.00 e 1-130 [» ]
    4B6A electron microscopy 8.10 e 1-130 [» ]
    4BYN electron microscopy 4.30 e 2-130 [» ]
    4BYU electron microscopy 6.60 e 2-130 [» ]
    4CUW electron microscopy 3.70 e 1-130 [» ]
    ProteinModelPortali P38061.
    SMRi P38061. Positions 2-128.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32611. 70 interactions.
    DIPi DIP-5176N.
    IntActi P38061. 7 interactions.
    MINTi MINT-8285132.

    Proteomic databases

    MaxQBi P38061.
    PaxDbi P38061.
    PeptideAtlasi P38061.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL092W ; YBL092W ; YBL092W .
    GeneIDi 852185.
    KEGGi sce:YBL092W.

    Organism-specific databases

    CYGDi YBL092w.
    SGDi S000000188. RPL32.

    Phylogenomic databases

    eggNOGi COG1717.
    GeneTreei ENSGT00390000014729.
    HOGENOMi HOG000231288.
    KOi K02912.
    OMAi TRHMLPT.
    OrthoDBi EOG7H79FB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28980-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    EvolutionaryTracei P38061.
    NextBioi 970652.
    PROi P38061.

    Gene expression databases

    Genevestigatori P38061.

    Family and domain databases

    InterProi IPR001515. Ribosomal_L32e.
    IPR018263. Ribosomal_L32e_CS.
    [Graphical view ]
    Pfami PF01655. Ribosomal_L32e. 1 hit.
    [Graphical view ]
    ProDomi PD003823. Ribosomal_L32e. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF52042. SSF52042. 1 hit.
    PROSITEi PS00580. RIBOSOMAL_L32E. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
      Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
      Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
      Juneau K., Palm C., Miranda M., Davis R.W.
      Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-76.
      Strain: ATCC 201390 / BY4743.
    6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    7. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
      Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
      J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE.
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 14-122, ELECTRON MICROSCOPY.
    10. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
    11. "Crystal structure of the eukaryotic ribosome."
      Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
      Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
    12. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

    Entry informationi

    Entry nameiRL32_YEAST
    AccessioniPrimary (citable) accession number: P38061
    Secondary accession number(s): A2TBM1, D6VPR1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3