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Protein

60S ribosomal protein L32

Gene

RPL32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-28980-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L32
Gene namesi
Name:RPL32
Ordered Locus Names:YBL092W
ORF Names:YBL0838
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome II

Organism-specific databases

CYGDiYBL092w.
SGDiS000000188. RPL32.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 13012960S ribosomal protein L32PRO_0000131145Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei40 – 401Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38061.
PaxDbiP38061.
PeptideAtlasiP38061.

Expressioni

Gene expression databases

GenevestigatoriP38061.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi32611. 71 interactions.
DIPiDIP-5176N.
IntActiP38061. 7 interactions.
MINTiMINT-8285132.

Structurei

Secondary structure

1
130
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni20 – 245Combined sources
Beta strandi37 – 393Combined sources
Turni41 – 455Combined sources
Helixi55 – 573Combined sources
Turni61 – 655Combined sources
Beta strandi68 – 703Combined sources
Beta strandi72 – 787Combined sources
Helixi79 – 824Combined sources
Helixi83 – 853Combined sources
Turni89 – 913Combined sources
Beta strandi92 – 965Combined sources
Helixi102 – 11514Combined sources
Beta strandi118 – 1203Combined sources
Turni122 – 1254Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-X14-122[»]
3J6Xelectron microscopy6.10721-130[»]
3J6Yelectron microscopy6.10721-130[»]
3J77electron microscopy6.20821-130[»]
3J78electron microscopy6.30821-130[»]
4U3MX-ray3.00O2/o22-130[»]
4U3NX-ray3.20O2/o22-130[»]
4U3UX-ray2.90O2/o22-130[»]
4U4NX-ray3.10O2/o22-130[»]
4U4OX-ray3.60O2/o22-130[»]
4U4QX-ray3.00O2/o22-130[»]
4U4RX-ray2.80O2/o22-130[»]
4U4UX-ray3.00O2/o22-130[»]
4U4YX-ray3.20O2/o22-130[»]
4U4ZX-ray3.10O2/o22-130[»]
4U50X-ray3.20O2/o22-130[»]
4U51X-ray3.20O2/o22-130[»]
4U52X-ray3.00O2/o22-130[»]
4U53X-ray3.30O2/o22-130[»]
4U55X-ray3.20O2/o22-130[»]
4U56X-ray3.45O2/o22-130[»]
4U6FX-ray3.10O2/o22-130[»]
4V4Belectron microscopy11.70B01-130[»]
4V6Ielectron microscopy8.80Bh1-130[»]
4V7Felectron microscopy8.70d1-130[»]
4V7RX-ray4.00Bb/Db1-130[»]
4V88X-ray3.00Be/De1-130[»]
4V8Telectron microscopy8.10e1-130[»]
4V8Yelectron microscopy4.30Be2-130[»]
4V8Zelectron microscopy6.60Be2-130[»]
4V91electron microscopy3.70e1-130[»]
ProteinModelPortaliP38061.
SMRiP38061. Positions 2-128.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38061.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L32e family.Curated

Phylogenomic databases

eggNOGiCOG1717.
GeneTreeiENSGT00390000014729.
HOGENOMiHOG000231288.
InParanoidiP38061.
KOiK02912.
OMAiQSDRYDK.
OrthoDBiEOG7H79FB.

Family and domain databases

InterProiIPR001515. Ribosomal_L32e.
IPR018263. Ribosomal_L32e_CS.
[Graphical view]
PfamiPF01655. Ribosomal_L32e. 1 hit.
[Graphical view]
ProDomiPD003823. Ribosomal_L32e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52042. SSF52042. 1 hit.
PROSITEiPS00580. RIBOSOMAL_L32E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLPHPKIV KKHTKKFKRH HSDRYHRVAE NWRKQKGIDS VVRRRFRGNI
60 70 80 90 100
SQPKIGYGSN KKTKFLSPSG HKTFLVANVK DLETLTMHTK TYAAEIAHNI
110 120 130
SAKNRVVILA RAKALGIKVT NPKGRLALEA
Length:130
Mass (Da):14,771
Last modified:October 1, 1994 - v1
Checksum:i5C5EDD87D6841976
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79489 Genomic DNA. Translation: CAA56010.1.
Z35853 Genomic DNA. Translation: CAA84914.1.
AY558291 Genomic DNA. Translation: AAS56617.1.
EF123124 mRNA. Translation: ABM97468.1.
BK006936 Genomic DNA. Translation: DAA07031.1.
PIRiS45410.
RefSeqiNP_009460.1. NM_001178332.1.

Genome annotation databases

EnsemblFungiiYBL092W; YBL092W; YBL092W.
GeneIDi852185.
KEGGisce:YBL092W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79489 Genomic DNA. Translation: CAA56010.1.
Z35853 Genomic DNA. Translation: CAA84914.1.
AY558291 Genomic DNA. Translation: AAS56617.1.
EF123124 mRNA. Translation: ABM97468.1.
BK006936 Genomic DNA. Translation: DAA07031.1.
PIRiS45410.
RefSeqiNP_009460.1. NM_001178332.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-X14-122[»]
3J6Xelectron microscopy6.10721-130[»]
3J6Yelectron microscopy6.10721-130[»]
3J77electron microscopy6.20821-130[»]
3J78electron microscopy6.30821-130[»]
4U3MX-ray3.00O2/o22-130[»]
4U3NX-ray3.20O2/o22-130[»]
4U3UX-ray2.90O2/o22-130[»]
4U4NX-ray3.10O2/o22-130[»]
4U4OX-ray3.60O2/o22-130[»]
4U4QX-ray3.00O2/o22-130[»]
4U4RX-ray2.80O2/o22-130[»]
4U4UX-ray3.00O2/o22-130[»]
4U4YX-ray3.20O2/o22-130[»]
4U4ZX-ray3.10O2/o22-130[»]
4U50X-ray3.20O2/o22-130[»]
4U51X-ray3.20O2/o22-130[»]
4U52X-ray3.00O2/o22-130[»]
4U53X-ray3.30O2/o22-130[»]
4U55X-ray3.20O2/o22-130[»]
4U56X-ray3.45O2/o22-130[»]
4U6FX-ray3.10O2/o22-130[»]
4V4Belectron microscopy11.70B01-130[»]
4V6Ielectron microscopy8.80Bh1-130[»]
4V7Felectron microscopy8.70d1-130[»]
4V7RX-ray4.00Bb/Db1-130[»]
4V88X-ray3.00Be/De1-130[»]
4V8Telectron microscopy8.10e1-130[»]
4V8Yelectron microscopy4.30Be2-130[»]
4V8Zelectron microscopy6.60Be2-130[»]
4V91electron microscopy3.70e1-130[»]
ProteinModelPortaliP38061.
SMRiP38061. Positions 2-128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32611. 71 interactions.
DIPiDIP-5176N.
IntActiP38061. 7 interactions.
MINTiMINT-8285132.

Proteomic databases

MaxQBiP38061.
PaxDbiP38061.
PeptideAtlasiP38061.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL092W; YBL092W; YBL092W.
GeneIDi852185.
KEGGisce:YBL092W.

Organism-specific databases

CYGDiYBL092w.
SGDiS000000188. RPL32.

Phylogenomic databases

eggNOGiCOG1717.
GeneTreeiENSGT00390000014729.
HOGENOMiHOG000231288.
InParanoidiP38061.
KOiK02912.
OMAiQSDRYDK.
OrthoDBiEOG7H79FB.

Enzyme and pathway databases

BioCyciYEAST:G3O-28980-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.
REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_252688. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_257951. Peptide chain elongation.

Miscellaneous databases

EvolutionaryTraceiP38061.
NextBioi970652.
PROiP38061.

Gene expression databases

GenevestigatoriP38061.

Family and domain databases

InterProiIPR001515. Ribosomal_L32e.
IPR018263. Ribosomal_L32e_CS.
[Graphical view]
PfamiPF01655. Ribosomal_L32e. 1 hit.
[Graphical view]
ProDomiPD003823. Ribosomal_L32e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF52042. SSF52042. 1 hit.
PROSITEiPS00580. RIBOSOMAL_L32E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
    Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
    Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome II."
    Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
    , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
    EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing."
    Juneau K., Palm C., Miranda M., Davis R.W.
    Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-76.
    Strain: ATCC 201390 / BY4743.
  6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  7. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE OF INITIATOR METHIONINE.
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
    Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
    Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 14-122, ELECTRON MICROSCOPY.
  10. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
    Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
    EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING, ELECTRON MICROSCOPY.
  11. "Crystal structure of the eukaryotic ribosome."
    Ben-Shem A., Jenner L., Yusupova G., Yusupov M.
    Science 330:1203-1209(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 80S RIBOSOME.
  12. Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 80S RIBOSOME.

Entry informationi

Entry nameiRL32_YEAST
AccessioniPrimary (citable) accession number: P38061
Secondary accession number(s): A2TBM1, D6VPR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: March 4, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.