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Protein

60S ribosomal protein L32

Gene

RPL32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-28980-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
60S ribosomal protein L32
Gene namesi
Name:RPL32
Ordered Locus Names:YBL092W
ORF Names:YBL0838
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBL092W.
SGDiS000000188. RPL32.

Subcellular locationi

GO - Cellular componenti

  • cytosolic large ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001311452 – 13060S ribosomal protein L32Add BLAST129

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei40PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP38061.
PRIDEiP38061.
TopDownProteomicsiP38061.

PTM databases

iPTMnetiP38061.

Interactioni

Subunit structurei

Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi32611. 30 interactors.
DIPiDIP-5176N.
IntActiP38061. 9 interactors.
MINTiMINT-8285132.

Structurei

Secondary structure

1130
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni20 – 24Combined sources5
Beta strandi37 – 39Combined sources3
Turni41 – 45Combined sources5
Helixi55 – 57Combined sources3
Turni61 – 65Combined sources5
Beta strandi68 – 70Combined sources3
Beta strandi72 – 78Combined sources7
Helixi79 – 82Combined sources4
Helixi83 – 85Combined sources3
Turni89 – 91Combined sources3
Beta strandi92 – 96Combined sources5
Helixi102 – 115Combined sources14
Beta strandi118 – 120Combined sources3
Turni122 – 125Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-X14-122[»]
3J6Xelectron microscopy6.10721-130[»]
3J6Yelectron microscopy6.10721-130[»]
3J77electron microscopy6.20821-130[»]
3J78electron microscopy6.30821-130[»]
3JCTelectron microscopy3.08e1-130[»]
4U3MX-ray3.00O2/o22-130[»]
4U3NX-ray3.20O2/o22-130[»]
4U3UX-ray2.90O2/o22-130[»]
4U4NX-ray3.10O2/o22-130[»]
4U4OX-ray3.60O2/o22-130[»]
4U4QX-ray3.00O2/o22-130[»]
4U4RX-ray2.80O2/o22-130[»]
4U4UX-ray3.00O2/o22-130[»]
4U4YX-ray3.20O2/o22-130[»]
4U4ZX-ray3.10O2/o22-130[»]
4U50X-ray3.20O2/o22-130[»]
4U51X-ray3.20O2/o22-130[»]
4U52X-ray3.00O2/o22-130[»]
4U53X-ray3.30O2/o22-130[»]
4U55X-ray3.20O2/o22-130[»]
4U56X-ray3.45O2/o22-130[»]
4U6FX-ray3.10O2/o22-130[»]
4V4Belectron microscopy11.70B01-130[»]
4V6Ielectron microscopy8.80Bh1-130[»]
4V7Felectron microscopy8.70d1-130[»]
4V7RX-ray4.00Bb/Db1-130[»]
4V88X-ray3.00Be/De1-130[»]
4V8Telectron microscopy8.10e1-130[»]
4V8Yelectron microscopy4.30Be2-130[»]
4V8Zelectron microscopy6.60Be2-130[»]
4V91electron microscopy3.70e1-130[»]
5APNelectron microscopy3.91e1-130[»]
5APOelectron microscopy3.41e1-130[»]
5DATX-ray3.15O2/o22-130[»]
5DC3X-ray3.25O2/o22-130[»]
5FCIX-ray3.40O2/o22-130[»]
5FCJX-ray3.10O2/o22-130[»]
5FL8electron microscopy9.50e1-130[»]
5GAKelectron microscopy3.88g1-130[»]
5I4LX-ray3.10O2/o22-128[»]
5JUOelectron microscopy4.00JA1-130[»]
5JUPelectron microscopy3.50JA1-130[»]
5JUSelectron microscopy4.20JA1-130[»]
5JUTelectron microscopy4.00JA1-130[»]
5JUUelectron microscopy4.00JA1-130[»]
ProteinModelPortaliP38061.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP38061.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L32e family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014729.
HOGENOMiHOG000231288.
InParanoidiP38061.
KOiK02912.
OMAiQKGIDSC.
OrthoDBiEOG092C5E5Z.

Family and domain databases

CDDicd00513. Ribosomal_L32_L32e. 1 hit.
InterProiIPR001515. Ribosomal_L32e.
IPR018263. Ribosomal_L32e_CS.
[Graphical view]
PfamiPF01655. Ribosomal_L32e. 1 hit.
[Graphical view]
ProDomiPD003823. Ribosomal_L32e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM01393. Ribosomal_L32e. 1 hit.
[Graphical view]
SUPFAMiSSF52042. SSF52042. 1 hit.
PROSITEiPS00580. RIBOSOMAL_L32E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38061-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLPHPKIV KKHTKKFKRH HSDRYHRVAE NWRKQKGIDS VVRRRFRGNI
60 70 80 90 100
SQPKIGYGSN KKTKFLSPSG HKTFLVANVK DLETLTMHTK TYAAEIAHNI
110 120 130
SAKNRVVILA RAKALGIKVT NPKGRLALEA
Length:130
Mass (Da):14,771
Last modified:October 1, 1994 - v1
Checksum:i5C5EDD87D6841976
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79489 Genomic DNA. Translation: CAA56010.1.
Z35853 Genomic DNA. Translation: CAA84914.1.
AY558291 Genomic DNA. Translation: AAS56617.1.
EF123124 mRNA. Translation: ABM97468.1.
BK006936 Genomic DNA. Translation: DAA07031.1.
PIRiS45410.
RefSeqiNP_009460.1. NM_001178332.1.

Genome annotation databases

EnsemblFungiiYBL092W; YBL092W; YBL092W.
GeneIDi852185.
KEGGisce:YBL092W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79489 Genomic DNA. Translation: CAA56010.1.
Z35853 Genomic DNA. Translation: CAA84914.1.
AY558291 Genomic DNA. Translation: AAS56617.1.
EF123124 mRNA. Translation: ABM97468.1.
BK006936 Genomic DNA. Translation: DAA07031.1.
PIRiS45410.
RefSeqiNP_009460.1. NM_001178332.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K5Ymodel-X14-122[»]
3J6Xelectron microscopy6.10721-130[»]
3J6Yelectron microscopy6.10721-130[»]
3J77electron microscopy6.20821-130[»]
3J78electron microscopy6.30821-130[»]
3JCTelectron microscopy3.08e1-130[»]
4U3MX-ray3.00O2/o22-130[»]
4U3NX-ray3.20O2/o22-130[»]
4U3UX-ray2.90O2/o22-130[»]
4U4NX-ray3.10O2/o22-130[»]
4U4OX-ray3.60O2/o22-130[»]
4U4QX-ray3.00O2/o22-130[»]
4U4RX-ray2.80O2/o22-130[»]
4U4UX-ray3.00O2/o22-130[»]
4U4YX-ray3.20O2/o22-130[»]
4U4ZX-ray3.10O2/o22-130[»]
4U50X-ray3.20O2/o22-130[»]
4U51X-ray3.20O2/o22-130[»]
4U52X-ray3.00O2/o22-130[»]
4U53X-ray3.30O2/o22-130[»]
4U55X-ray3.20O2/o22-130[»]
4U56X-ray3.45O2/o22-130[»]
4U6FX-ray3.10O2/o22-130[»]
4V4Belectron microscopy11.70B01-130[»]
4V6Ielectron microscopy8.80Bh1-130[»]
4V7Felectron microscopy8.70d1-130[»]
4V7RX-ray4.00Bb/Db1-130[»]
4V88X-ray3.00Be/De1-130[»]
4V8Telectron microscopy8.10e1-130[»]
4V8Yelectron microscopy4.30Be2-130[»]
4V8Zelectron microscopy6.60Be2-130[»]
4V91electron microscopy3.70e1-130[»]
5APNelectron microscopy3.91e1-130[»]
5APOelectron microscopy3.41e1-130[»]
5DATX-ray3.15O2/o22-130[»]
5DC3X-ray3.25O2/o22-130[»]
5FCIX-ray3.40O2/o22-130[»]
5FCJX-ray3.10O2/o22-130[»]
5FL8electron microscopy9.50e1-130[»]
5GAKelectron microscopy3.88g1-130[»]
5I4LX-ray3.10O2/o22-128[»]
5JUOelectron microscopy4.00JA1-130[»]
5JUPelectron microscopy3.50JA1-130[»]
5JUSelectron microscopy4.20JA1-130[»]
5JUTelectron microscopy4.00JA1-130[»]
5JUUelectron microscopy4.00JA1-130[»]
ProteinModelPortaliP38061.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32611. 30 interactors.
DIPiDIP-5176N.
IntActiP38061. 9 interactors.
MINTiMINT-8285132.

PTM databases

iPTMnetiP38061.

Proteomic databases

MaxQBiP38061.
PRIDEiP38061.
TopDownProteomicsiP38061.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBL092W; YBL092W; YBL092W.
GeneIDi852185.
KEGGisce:YBL092W.

Organism-specific databases

EuPathDBiFungiDB:YBL092W.
SGDiS000000188. RPL32.

Phylogenomic databases

GeneTreeiENSGT00390000014729.
HOGENOMiHOG000231288.
InParanoidiP38061.
KOiK02912.
OMAiQKGIDSC.
OrthoDBiEOG092C5E5Z.

Enzyme and pathway databases

BioCyciYEAST:G3O-28980-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

EvolutionaryTraceiP38061.
PROiP38061.

Family and domain databases

CDDicd00513. Ribosomal_L32_L32e. 1 hit.
InterProiIPR001515. Ribosomal_L32e.
IPR018263. Ribosomal_L32e_CS.
[Graphical view]
PfamiPF01655. Ribosomal_L32e. 1 hit.
[Graphical view]
ProDomiPD003823. Ribosomal_L32e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM01393. Ribosomal_L32e. 1 hit.
[Graphical view]
SUPFAMiSSF52042. SSF52042. 1 hit.
PROSITEiPS00580. RIBOSOMAL_L32E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRL32_YEAST
AccessioniPrimary (citable) accession number: P38061
Secondary accession number(s): A2TBM1, D6VPR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.