P38056 (ALR_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	ML0375
ORF Names:	B229_C3_243

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Alanine racemase HAMAP MF_01201		PRO_0000114537
Sites
	Active site	44	1	Proton acceptor; specific for D-alanine By similarity
	Active site	273	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	44	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P38056 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: E4075B683D00D196	FASTA	388	41,083
	10 20 30 40 50 60 MAVTPISLRP GVLAEAVVDL GAIDYNVRVL REHAGMAQLM VVLKADAYGH GATQVALAAL 70 80 90 100 110 120 AAGAAELGVA TVDEALALRA DGISAPVLAW LHPPGIDFGP ALLADVQIAV SSVRQLDELL 130 140 150 160 170 180 DAVRRTGRTA TVTVKADTGL NRNGVVTDQY PAMLTALQRA VVEDAVRLRG LMSHLVYADQ 190 200 210 220 230 240 PDNPSNDVQG KRFAALLAQA HEQGLRFEVA HLSNSSATMS RPDLAYDLVR PGIAVYGLSP 250 260 270 280 290 300 VPSRGDMGLI PAMTVKCAVA MVKSIRAGEG VSYGHDWIAQ HDTNLALLPV GYADGVFRSL 310 320 330 340 350 360 GGRLDVLING KRRPGVGRIC MDQFVVDLGP GPIDVAEGDE AILFGPGARG EPTAQDWADL 370 380 LGTIHYEVVT SLRGRITRTY REAQTVDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Smith D.R., Robison K. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00020 Genomic DNA. Translation: AAA17309.1. AL583918 Genomic DNA. Translation: CAC29883.1.
PIR	S72995.
RefSeq	NP_301367.1. NC_002677.1.
3D structure databases
ProteinModelPortal	P38056.
SMR	P38056. Positions 13-383.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000029360; EBMYCP00000028966; EBMYCG00000029355.
GeneID	909060.
GenomeReviews	Gene locus ML0375 in contig AL450380_GR.
KEGG	mle:ML0375.
NMPDR	fig|272631.1.peg.239.
PATRIC	18051376. VBIMycLep78757_0637.
Organism-specific databases
Leproma	ML0375.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016627.
HOGENOM	HBG712172.
OMA	EGTPVWV.
ProtClustDB	PRK00053.
Enzyme and pathway databases
BioCyc	MLEP272631:ML0375-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_MYCLE

Accession

Primary (citable) accession number: P38056

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program