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P38041

- BOB1_YEAST

UniProt

P38041 - BOB1_YEAST

Protein

Protein BOB1

Gene

BOI1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Binds to the BEM1 protein.

    GO - Molecular functioni

    1. phospholipid binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. budding cell apical bud growth Source: SGD
    2. cytokinesis, completion of separation Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28974-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein BOB1
    Alternative name(s):
    BEM1-binding protein
    Growth inhibitory protein 7
    Gene namesi
    Name:BOI1
    Synonyms:BOB1, GIN7
    Ordered Locus Names:YBL085W
    ORF Names:YBL0717
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBL085w.
    SGDiS000000181. BOI1.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud Source: SGD
    2. cellular bud neck Source: SGD
    3. site of polarized growth Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 980980Protein BOB1PRO_0000064968Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei104 – 1041Phosphoserine2 Publications
    Modified residuei106 – 1061Phosphoserine1 Publication
    Modified residuei128 – 1281Phosphoserine1 Publication
    Modified residuei151 – 1511Phosphothreonine1 Publication
    Modified residuei158 – 1581Phosphothreonine1 Publication
    Modified residuei209 – 2091Phosphoserine3 Publications
    Modified residuei393 – 3931Phosphoserine1 Publication
    Modified residuei412 – 4121Phosphoserine1 Publication
    Modified residuei525 – 5251Phosphoserine1 Publication
    Modified residuei528 – 5281Phosphoserine2 Publications
    Modified residuei589 – 5891Phosphoserine1 Publication
    Modified residuei590 – 5901Phosphoserine1 Publication
    Modified residuei593 – 5931Phosphoserine1 Publication
    Modified residuei644 – 6441Phosphoserine2 Publications
    Modified residuei655 – 6551Phosphoserine1 Publication
    Modified residuei735 – 7351Phosphoserine1 Publication
    Modified residuei919 – 9191Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP38041.
    PaxDbiP38041.
    PRIDEiP38041.

    Expressioni

    Gene expression databases

    GenevestigatoriP38041.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BEM1P293665EBI-3719,EBI-3508
    CDC14Q006842EBI-3719,EBI-4192
    FIR1P400202EBI-3719,EBI-13431
    NBA1Q082293EBI-3719,EBI-36841
    YDR239CQ037802EBI-3719,EBI-30094
    YER158CP400954EBI-3719,EBI-22734
    YJR056CP471153EBI-3719,EBI-25514
    ZDS2P547863EBI-3719,EBI-29637

    Protein-protein interaction databases

    BioGridi32619. 91 interactions.
    DIPiDIP-2226N.
    IntActiP38041. 72 interactions.
    MINTiMINT-584486.
    STRINGi4932.YBL085W.

    Structurei

    3D structure databases

    ProteinModelPortaliP38041.
    SMRiP38041. Positions 14-75, 781-902.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 7765SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini228 – 29265SAMPROSITE-ProRule annotationAdd
    BLAST
    Domaini776 – 895120PHPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiNOG73665.
    GeneTreeiENSGT00530000066577.
    HOGENOMiHOG000066063.
    OMAiYVQRPST.
    OrthoDBiEOG7N63W3.

    Family and domain databases

    Gene3Di1.10.150.50. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00169. PH. 1 hit.
    PF07647. SAM_2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    SMARTiSM00233. PH. 1 hit.
    SM00454. SAM. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEiPS50003. PH_DOMAIN. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P38041-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLEGNTLGK GAKSFPLYIA VNQYSKRMED ELNMKPGDKI KVITDDGEYN    50
    DGWYYGRNLR TKEEGLYPAV FTKRIAIEKP ENLHKSPTQE SGNSGVKYGN 100
    LNDSASNIGK VSSHQQENRY TSLKSTMSDI DKALEELRSG SVEQEVSKSP 150
    TRVPEVSTPQ LQDEQTLIQE KTRNEENTTH DSLFSSTADL NLSSESLKNI 200
    SKSNISTKSL EPSSESVRQL DLKMAKSWSP EEVTDYFSLV GFDQSTCNKF 250
    KEHQVSGKIL LELELEHLKE LEINSFGIRF QIFKEIRNIK SAIDSSSNKL 300
    DADYSTFAFE NQAAQLMPAA TVNRDEIQQQ ISSKCNKLSS ESSDRKSSSV 350
    TTELQRPSSV VVNPNFKLHD PAEQILDMTE VPNLFADKDI FESPGRAPKP 400
    PSYPSPVQPP QSPSFNNRYT NNNARFPPQT TYPPKNKNPT VYSNGLIPNS 450
    STSSDNSTGK FKFPAMNGHD SNSRKTTLTS ATIPSINTVN TDESLPAISN 500
    ISSNATSHHP NRNSVVYNNH KRTESGSSFV DLFNRISMLS PVKSSFDEEE 550
    TKQPSKASRA VFDSARRKSS YGHSRDASLS EMKKHRRNSS ILSFFSSKSQ 600
    SNPTSPTKQT FTIDPAKMTS HSRSQSNSYS HARSQSYSHS RKHSLVTSPL 650
    KTSLSPINSK SNIALAHSET PTSSNNKEAV SQPSEGKHKH KHKHKSKHKH 700
    KNSSSKDGSS EEKSKKKLFS STKESFVGSK EFKRSPSELT QKSTKSILPR 750
    SNAKKQQTSA FTEGIRSITA KESMQTADCS GWMSKKGTGA MGTWKQRFFT 800
    LHGTRLSYFT NTNDEKERGL IDITAHRVLP ASDDDRLISL YAASLGKGKY 850
    CFKLVPPQPG SKKGLTFTEP RVHYFAVENK SEMKAWLSAI IKATIDIDTS 900
    VPVISSYATP TIPLSKAQTL LEEARLQTQL RDAEEEEGRD QFGWDDTQNK 950
    RNSNYPIEQD QFETSDYLES SAFEYPGGRL 980
    Length:980
    Mass (Da):109,295
    Last modified:October 1, 1994 - v1
    Checksum:i09F1DD1F9EF30F36
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L31406 Genomic DNA. Translation: AAB08439.1.
    X79489 Genomic DNA. Translation: CAA56021.1.
    Z35846 Genomic DNA. Translation: CAA84906.1.
    BK006936 Genomic DNA. Translation: DAA07039.1.
    PIRiS45444.
    RefSeqiNP_009468.1. NM_001178325.1.

    Genome annotation databases

    EnsemblFungiiYBL085W; YBL085W; YBL085W.
    GeneIDi852193.
    KEGGisce:YBL085W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L31406 Genomic DNA. Translation: AAB08439.1 .
    X79489 Genomic DNA. Translation: CAA56021.1 .
    Z35846 Genomic DNA. Translation: CAA84906.1 .
    BK006936 Genomic DNA. Translation: DAA07039.1 .
    PIRi S45444.
    RefSeqi NP_009468.1. NM_001178325.1.

    3D structure databases

    ProteinModelPortali P38041.
    SMRi P38041. Positions 14-75, 781-902.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32619. 91 interactions.
    DIPi DIP-2226N.
    IntActi P38041. 72 interactions.
    MINTi MINT-584486.
    STRINGi 4932.YBL085W.

    Proteomic databases

    MaxQBi P38041.
    PaxDbi P38041.
    PRIDEi P38041.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBL085W ; YBL085W ; YBL085W .
    GeneIDi 852193.
    KEGGi sce:YBL085W.

    Organism-specific databases

    CYGDi YBL085w.
    SGDi S000000181. BOI1.

    Phylogenomic databases

    eggNOGi NOG73665.
    GeneTreei ENSGT00530000066577.
    HOGENOMi HOG000066063.
    OMAi YVQRPST.
    OrthoDBi EOG7N63W3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28974-MONOMER.

    Miscellaneous databases

    NextBioi 970676.

    Gene expression databases

    Genevestigatori P38041.

    Family and domain databases

    Gene3Di 1.10.150.50. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00169. PH. 1 hit.
    PF07647. SAM_2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    SMARTi SM00233. PH. 1 hit.
    SM00454. SAM. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEi PS50003. PH_DOMAIN. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Associations among PH and SH3 domain-containing proteins and Rho-type GTPases in Yeast."
      Bender L., Lo H.S., Lee H., Kokojan V., Peterson V., Bender A.
      J. Cell Biol. 133:879-894(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II."
      Obermaier B., Gassenhuber J., Piravandi E., Domdey H.
      Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-209, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; SER-209; SER-528; SER-644 AND THR-919, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-735, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; SER-106; SER-128; THR-151; THR-158; SER-209; SER-393; SER-412; SER-525; SER-528; SER-589; SER-590; SER-593 AND SER-644, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBOB1_YEAST
    AccessioniPrimary (citable) accession number: P38041
    Secondary accession number(s): D6VPR9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1040 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3