P38041 (BOB1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein BOB1

Alternative name(s):
BEM1-binding protein
Growth inhibitory protein 7

Gene names

Name:	BOI1
Synonyms:	BOB1, GIN7
Ordered Locus Names:	YBL085W
ORF Names:	YBL0717

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	980 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the BEM1 protein.
Miscellaneous	Present with 1040 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 PH domain. Contains 1 SAM (sterile alpha motif) domain. Contains 1 SH3 domain.

Ontologies

Keywords
Domain	SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rho protein signal transduction Traceable author statement PubMed 10639324. Source: SGD budding cell apical bud growth Inferred from genetic interaction PubMed 17417630. Source: SGD cytokinesis, completion of separation Inferred from genetic interaction PubMed 16615892. Source: SGD establishment of cell polarity Traceable author statement PubMed 10639324. Source: SGD
Cellular_component	cellular bud neck Inferred from direct assay PubMed 12097146PubMed 22842922. Source: SGD cytoplasm Inferred from direct assay PubMed 22842922. Source: SGD mating projection tip Inferred from direct assay PubMed 19053807. Source: SGD plasma membrane Inferred from direct assay PubMed 22842922. Source: SGD
Molecular_function	phospholipid binding Inferred from direct assay PubMed 12097146. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDC14	Q00684	2	EBI-3719,EBI-4192
YER158C	P40095	2	EBI-3719,EBI-22734

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 980	980	Protein BOB1		PRO_0000064968
Regions
	Domain	13 – 77	65	SH3
	Domain	228 – 292	65	SAM
	Domain	776 – 895	120	PH
Amino acid modifications
	Modified residue	104	1	Phosphoserine Ref.7 Ref.10
	Modified residue	157	1	Phosphoserine Ref.10
	Modified residue	158	1	Phosphothreonine Ref.10
	Modified residue	206	1	Phosphoserine Ref.10
	Modified residue	207	1	Phosphothreonine Ref.6 Ref.10
	Modified residue	209	1	Phosphoserine Ref.6 Ref.7 Ref.10
	Modified residue	525	1	Phosphoserine Ref.6
	Modified residue	528	1	Phosphoserine Ref.7 Ref.10
	Modified residue	590	1	Phosphoserine Ref.6
	Modified residue	644	1	Phosphoserine Ref.7
	Modified residue	655	1	Phosphoserine Ref.6 Ref.10
	Modified residue	735	1	Phosphoserine Ref.8
	Modified residue	737	1	Phosphoserine Ref.9
	Modified residue	919	1	Phosphothreonine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P38041 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 09F1DD1F9EF30F36	FASTA	980	109,295
	10 20 30 40 50 60 MSLEGNTLGK GAKSFPLYIA VNQYSKRMED ELNMKPGDKI KVITDDGEYN DGWYYGRNLR 70 80 90 100 110 120 TKEEGLYPAV FTKRIAIEKP ENLHKSPTQE SGNSGVKYGN LNDSASNIGK VSSHQQENRY 130 140 150 160 170 180 TSLKSTMSDI DKALEELRSG SVEQEVSKSP TRVPEVSTPQ LQDEQTLIQE KTRNEENTTH 190 200 210 220 230 240 DSLFSSTADL NLSSESLKNI SKSNISTKSL EPSSESVRQL DLKMAKSWSP EEVTDYFSLV 250 260 270 280 290 300 GFDQSTCNKF KEHQVSGKIL LELELEHLKE LEINSFGIRF QIFKEIRNIK SAIDSSSNKL 310 320 330 340 350 360 DADYSTFAFE NQAAQLMPAA TVNRDEIQQQ ISSKCNKLSS ESSDRKSSSV TTELQRPSSV 370 380 390 400 410 420 VVNPNFKLHD PAEQILDMTE VPNLFADKDI FESPGRAPKP PSYPSPVQPP QSPSFNNRYT 430 440 450 460 470 480 NNNARFPPQT TYPPKNKNPT VYSNGLIPNS STSSDNSTGK FKFPAMNGHD SNSRKTTLTS 490 500 510 520 530 540 ATIPSINTVN TDESLPAISN ISSNATSHHP NRNSVVYNNH KRTESGSSFV DLFNRISMLS 550 560 570 580 590 600 PVKSSFDEEE TKQPSKASRA VFDSARRKSS YGHSRDASLS EMKKHRRNSS ILSFFSSKSQ 610 620 630 640 650 660 SNPTSPTKQT FTIDPAKMTS HSRSQSNSYS HARSQSYSHS RKHSLVTSPL KTSLSPINSK 670 680 690 700 710 720 SNIALAHSET PTSSNNKEAV SQPSEGKHKH KHKHKSKHKH KNSSSKDGSS EEKSKKKLFS 730 740 750 760 770 780 STKESFVGSK EFKRSPSELT QKSTKSILPR SNAKKQQTSA FTEGIRSITA KESMQTADCS 790 800 810 820 830 840 GWMSKKGTGA MGTWKQRFFT LHGTRLSYFT NTNDEKERGL IDITAHRVLP ASDDDRLISL 850 860 870 880 890 900 YAASLGKGKY CFKLVPPQPG SKKGLTFTEP RVHYFAVENK SEMKAWLSAI IKATIDIDTS 910 920 930 940 950 960 VPVISSYATP TIPLSKAQTL LEEARLQTQL RDAEEEEGRD QFGWDDTQNK RNSNYPIEQD 970 980 QFETSDYLES SAFEYPGGRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Associations among PH and SH3 domain-containing proteins and Rho-type GTPases in Yeast." Bender L., Lo H.S., Lee H., Kokojan V., Peterson V., Bender A. J. Cell Biol. 133:879-894(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-207; SER-209; SER-525; SER-590 AND SER-655, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; SER-209; SER-528; SER-644 AND THR-919, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-735, MASS SPECTROMETRY.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-737, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; SER-157; THR-158; SER-206; THR-207; SER-209; SER-528 AND SER-655, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L31406 Genomic DNA. Translation: AAB08439.1. X79489 Genomic DNA. Translation: CAA56021.1. Z35846 Genomic DNA. Translation: CAA84906.1. BK006936 Genomic DNA. Translation: DAA07039.1.
PIR	S45444.
RefSeq	NP_009468.1. NM_001178325.1.
3D structure databases
ProteinModelPortal	P38041.
SMR	P38041. Positions 13-75, 781-902.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2226N.
IntAct	P38041. 32 interactions.
MINT	MINT-584486.
STRING	4932.YBL085W.
Proteomic databases
PaxDb	P38041.
PRIDE	P38041.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBL085W; YBL085W; YBL085W.
GeneID	852193.
KEGG	sce:YBL085W.
Organism-specific databases
CYGD	YBL085w.
SGD	S000000181. BOI1.
Phylogenomic databases
eggNOG	NOG73665.
GeneTree	ENSGT00530000066577.
HOGENOM	HOG000066063.
OMA	RISMLSP.
OrthoDB	EOG4BZR9T.
Gene expression databases
Genevestigator	P38041.
GermOnline	YBL085W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.150.50. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR001660. SAM. IPR013761. SAM/pointed. IPR011510. SAM_2. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00169. PH. 1 hit. PF07647. SAM_2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00454. SAM. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF47769. SAM_homology. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS50105. SAM_DOMAIN. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	970676.

Entry information

Entry name

BOB1_YEAST

Accession

Primary (citable) accession number: P38041
Secondary accession number(s): D6VPR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program