Reviewed,
UniProtKB/Swiss-Prot P38041 (BOB1_YEAST)
Last modified
November 3, 2009.
Version 91.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Protein BOB1 Alternative name(s): BEM1-binding protein Growth inhibitory protein 7 | ||||||||
| Gene names |
| ||||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 980 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Binds to the BEM1 protein. |
| Miscellaneous | Present with 1040 molecules/cell in log phase SD medium. Ref.4 |
| Sequence similarities | Contains 1 PH domain. Contains 1 SAM (sterile alpha motif) domain. Contains 1 SH3 domain. |
Ontologies
| Keywords | |
|---|---|
| Domain | SH3 domain |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | Rho protein signal transduction Traceable author statement. Source: SGD budding cell apical bud growthInferred from genetic interaction. Source: SGD cytokinesis, completion of separationInferred from genetic interaction. Source: SGD establishment of cell polarityTraceable author statement. Source: SGD |
| Cellular component | cellular bud neck Inferred from direct assay. Source: SGD mating projection tipInferred from direct assay. Source: SGD |
| Molecular function | identical protein binding Inferred from physical interaction. Source: IntAct phospholipid bindingInferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| itself | 1 | EBI-3719,EBI-3719 | ||
| O13561 | 1 | EBI-3719,EBI-31058 | ||
| P40095 | 1 | EBI-3719,EBI-22734 | ||
| Q03780 | 1 | EBI-3719,EBI-30094 | ||
| Q12510 | 1 | EBI-3719,EBI-35343 | ||
| ACF2 | Q12168 | 1 | EBI-3719,EBI-32973 | |
| BSP1 | Q06604 | 1 | EBI-3719,EBI-37047 | |
| DAL2 | P25335 | 1 | EBI-3719,EBI-2442 | |
| MSI1 | P13712 | 1 | EBI-3719,EBI-11391 | |
| NBA1 | Q08229 | 1 | EBI-3719,EBI-36841 | |
| PRP20 | P21827 | 1 | EBI-3719,EBI-14866 | |
| RPC53 | P25441 | 1 | EBI-3719,EBI-15826 | |
| RSM7 | P47150 | 1 | EBI-3719,EBI-25623 | |
| SMC4 | Q12267 | 1 | EBI-3719,EBI-17430 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 980 | 980 | Protein BOB1 | PRO_0000064968 | |||||
Regions | |||||||||
| Domain | 13 – 77 | 65 | SH3 | ||||||
| Domain | 228 – 292 | 65 | SAM | ||||||
| Domain | 776 – 895 | 120 | PH | ||||||
Amino acid modifications | |||||||||
| Modified residue | 104 | 1 | Phosphoserine Ref.6 Ref.9 | ||||||
| Modified residue | 157 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 158 | 1 | Phosphothreonine Ref.9 | ||||||
| Modified residue | 206 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 207 | 1 | Phosphothreonine Ref.9 Ref.5 | ||||||
| Modified residue | 209 | 1 | Phosphoserine Ref.6 Ref.9 Ref.5 | ||||||
| Modified residue | 525 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 528 | 1 | Phosphoserine Ref.6 Ref.9 | ||||||
| Modified residue | 590 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 644 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 655 | 1 | Phosphoserine Ref.9 Ref.5 | ||||||
| Modified residue | 735 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 737 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 919 | 1 | Phosphothreonine Ref.6 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Associations among PH and SH3 domain-containing proteins and Rho-type GTPases in Yeast." Bender L., Lo H.S., Lee H., Kokojan V., Peterson V., Bender A. J. Cell Biol. 133:879-894(1996) [PubMed: 8666672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K.EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [5] | "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-207; SER-209; SER-525; SER-590 AND SER-655, MASS SPECTROMETRY. |
| [6] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; SER-209; SER-528; SER-644 AND THR-919, MASS SPECTROMETRY. |
| [7] | "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-735, MASS SPECTROMETRY. |
| [8] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-737, MASS SPECTROMETRY. |
| [9] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; SER-157; THR-158; SER-206; THR-207; SER-209; SER-528 AND SER-655, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| L31406 Genomic DNA. Translation: AAB08439.1. X79489 Genomic DNA. Translation: CAA56021.1. Z35846 Genomic DNA. Translation: CAA84906.1. | |
| PIR | S45444. |
| RefSeq | NP_009468.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FHX based on UniProtKB O08967. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:2226N. |
| IntAct | P38041. 41 interactions. |
| STRING | P38041. |
Genome annotation databases | |
| Ensembl | YBL085W; YBL085W; YBL085W; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 852193. |
| GenomeReviews | Gene locus YBL085W in contig Y13134_GR. |
| KEGG | sce:YBL085W. |
| NMPDR | fig|4932.3.peg.154. |
Organism-specific databases | |
| CYGD | YBL085w. |
| SGD | S000000181. BOI1. |
Phylogenomic databases | |
| HOGENOM | P38041. |
| OMA | GSEYSSH. |
Gene expression databases | |
| ArrayExpress | P38041. |
| Genevestigator | P38041. |
| GermOnline | YBL085W. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR001660. SAM. IPR011510. SAM_2. IPR001452. SH3_domain. [Graphical view] |
| Gene3D | G3DSA:2.30.29.30. PH_type. 1 hit. |
| Pfam | PF00169. PH. 1 hit. PF07647. SAM_2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view] |
| ProDom | PD000066. SH3. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00233. PH. 1 hit. SM00454. SAM. 1 hit. SM00326. SH3. 1 hit. [Graphical view] |
| PROSITE | PS50003. PH_DOMAIN. 1 hit. PS50105. SAM_DOMAIN. 1 hit. PS50002. SH3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 970676. |
Entry information
| Entry name | BOB1_YEAST | ||||||||
| Accession | Primary (citable) accession number: P38041 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome II Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names |

Clusters with


