Reviewed, UniProtKB/Swiss-Prot P38041 (BOB1_YEAST)

Names and origin

Protein names

Recommended name:
    Protein BOB1
Alternative name(s):
    BEM1-binding protein
    Growth inhibitory protein 7

Gene names

Name:	BOI1
Synonyms:	BOB1, GIN7
Ordered Locus Names:	YBL085W
ORF Names:	YBL0717

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	980 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to the BEM1 protein.
Miscellaneous	Present with 1040 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Contains 1 PH domain. Contains 1 SAM (sterile alpha motif) domain. Contains 1 SH3 domain.

Ontologies

Keywords
Domain	SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Rho protein signal transduction Traceable author statement. Source: SGD budding cell apical bud growth Inferred from genetic interaction. Source: SGD cytokinesis, completion of separation Inferred from genetic interaction. Source: SGD establishment of cell polarity Traceable author statement. Source: SGD
Cellular component	cellular bud neck Inferred from direct assay. Source: SGD mating projection tip Inferred from direct assay. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct phospholipid binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-3719,EBI-3719
	O13561	1	EBI-3719,EBI-31058
	P40095	1	EBI-3719,EBI-22734
	Q03780	1	EBI-3719,EBI-30094
	Q12510	1	EBI-3719,EBI-35343
ACF2	Q12168	1	EBI-3719,EBI-32973
BSP1	Q06604	1	EBI-3719,EBI-37047
DAL2	P25335	1	EBI-3719,EBI-2442
MSI1	P13712	1	EBI-3719,EBI-11391
NBA1	Q08229	1	EBI-3719,EBI-36841
PRP20	P21827	1	EBI-3719,EBI-14866
RPC53	P25441	1	EBI-3719,EBI-15826
RSM7	P47150	1	EBI-3719,EBI-25623
SMC4	Q12267	1	EBI-3719,EBI-17430

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 980	980	Protein BOB1		PRO_0000064968
Regions
	Domain	13 – 77	65	SH3
	Domain	228 – 292	65	SAM
	Domain	776 – 895	120	PH
Amino acid modifications
	Modified residue	104	1	Phosphoserine Ref.6 Ref.9
	Modified residue	157	1	Phosphoserine Ref.9
	Modified residue	158	1	Phosphothreonine Ref.9
	Modified residue	206	1	Phosphoserine Ref.9
	Modified residue	207	1	Phosphothreonine Ref.9 Ref.5
	Modified residue	209	1	Phosphoserine Ref.6 Ref.9 Ref.5
	Modified residue	525	1	Phosphoserine Ref.5
	Modified residue	528	1	Phosphoserine Ref.6 Ref.9
	Modified residue	590	1	Phosphoserine Ref.5
	Modified residue	644	1	Phosphoserine Ref.6
	Modified residue	655	1	Phosphoserine Ref.9 Ref.5
	Modified residue	735	1	Phosphoserine Ref.7
	Modified residue	737	1	Phosphoserine Ref.8
	Modified residue	919	1	Phosphothreonine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P38041-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: 09F1DD1F9EF30F36	FASTA	980	109,295
	10 20 30 40 50 60 MSLEGNTLGK GAKSFPLYIA VNQYSKRMED ELNMKPGDKI KVITDDGEYN DGWYYGRNLR 70 80 90 100 110 120 TKEEGLYPAV FTKRIAIEKP ENLHKSPTQE SGNSGVKYGN LNDSASNIGK VSSHQQENRY 130 140 150 160 170 180 TSLKSTMSDI DKALEELRSG SVEQEVSKSP TRVPEVSTPQ LQDEQTLIQE KTRNEENTTH 190 200 210 220 230 240 DSLFSSTADL NLSSESLKNI SKSNISTKSL EPSSESVRQL DLKMAKSWSP EEVTDYFSLV 250 260 270 280 290 300 GFDQSTCNKF KEHQVSGKIL LELELEHLKE LEINSFGIRF QIFKEIRNIK SAIDSSSNKL 310 320 330 340 350 360 DADYSTFAFE NQAAQLMPAA TVNRDEIQQQ ISSKCNKLSS ESSDRKSSSV TTELQRPSSV 370 380 390 400 410 420 VVNPNFKLHD PAEQILDMTE VPNLFADKDI FESPGRAPKP PSYPSPVQPP QSPSFNNRYT 430 440 450 460 470 480 NNNARFPPQT TYPPKNKNPT VYSNGLIPNS STSSDNSTGK FKFPAMNGHD SNSRKTTLTS 490 500 510 520 530 540 ATIPSINTVN TDESLPAISN ISSNATSHHP NRNSVVYNNH KRTESGSSFV DLFNRISMLS 550 560 570 580 590 600 PVKSSFDEEE TKQPSKASRA VFDSARRKSS YGHSRDASLS EMKKHRRNSS ILSFFSSKSQ 610 620 630 640 650 660 SNPTSPTKQT FTIDPAKMTS HSRSQSNSYS HARSQSYSHS RKHSLVTSPL KTSLSPINSK 670 680 690 700 710 720 SNIALAHSET PTSSNNKEAV SQPSEGKHKH KHKHKSKHKH KNSSSKDGSS EEKSKKKLFS 730 740 750 760 770 780 STKESFVGSK EFKRSPSELT QKSTKSILPR SNAKKQQTSA FTEGIRSITA KESMQTADCS 790 800 810 820 830 840 GWMSKKGTGA MGTWKQRFFT LHGTRLSYFT NTNDEKERGL IDITAHRVLP ASDDDRLISL 850 860 870 880 890 900 YAASLGKGKY CFKLVPPQPG SKKGLTFTEP RVHYFAVENK SEMKAWLSAI IKATIDIDTS 910 920 930 940 950 960 VPVISSYATP TIPLSKAQTL LEEARLQTQL RDAEEEEGRD QFGWDDTQNK RNSNYPIEQD 970 980 QFETSDYLES SAFEYPGGRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Associations among PH and SH3 domain-containing proteins and Rho-type GTPases in Yeast." Bender L., Lo H.S., Lee H., Kokojan V., Peterson V., Bender A. J. Cell Biol. 133:879-894(1996) [PubMed: 8666672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II." Obermaier B., Gassenhuber J., Piravandi E., Domdey H. Yeast 11:1103-1112(1995) [PubMed: 7502586] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-207; SER-209; SER-525; SER-590 AND SER-655, MASS SPECTROMETRY.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; SER-209; SER-528; SER-644 AND THR-919, MASS SPECTROMETRY.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-735, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-737, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; SER-157; THR-158; SER-206; THR-207; SER-209; SER-528 AND SER-655, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L31406 Genomic DNA. Translation: AAB08439.1. X79489 Genomic DNA. Translation: CAA56021.1. Z35846 Genomic DNA. Translation: CAA84906.1.
PIR	S45444.
RefSeq	NP_009468.1.
3D structure databases
HSSP	HSSP built from PDB template 1FHX based on UniProtKB O08967.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2226N.
IntAct	P38041. 41 interactions.
STRING	P38041.
Genome annotation databases
Ensembl	YBL085W; YBL085W; YBL085W; Saccharomyces cerevisiae. [Genome view]
GeneID	852193.
GenomeReviews	Gene locus YBL085W in contig Y13134_GR.
KEGG	sce:YBL085W.
NMPDR	fig|4932.3.peg.154.
Organism-specific databases
CYGD	YBL085w.
SGD	S000000181. BOI1.
Phylogenomic databases
HOGENOM	P38041.
OMA	GSEYSSH.
Gene expression databases
ArrayExpress	P38041.
Genevestigator	P38041.
GermOnline	YBL085W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR001660. SAM. IPR011510. SAM_2. IPR001452. SH3_domain. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF00169. PH. 1 hit. PF07647. SAM_2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
ProDom	PD000066. SH3. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00233. PH. 1 hit. SM00454. SAM. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS50105. SAM_DOMAIN. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970676.

Entry information

Entry name

BOB1_YEAST

Accession

Primary (citable) accession number: P38041

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	November 3, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)