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Reviewed, UniProtKB/Swiss-Prot P38032 (NADB_BACSU)

Last modified June 16, 2009. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B
Gene names
Name: nadB
Ordered Locus Names: BSU27870
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length531 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the oxidation of L-aspartate to iminoaspartate.

Catalytic activity

L-aspartate + O2 = iminosuccinate + H2O2.

Cofactor

FAD.

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

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Ontologies

Keywords
   Biological processPyridine nucleotide biosynthesis
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processNAD biosynthetic process

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-aspartate oxidase activity

Inferred from electronic annotation. Source: EC

electron carrier activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 531531L-aspartate oxidase
PRO_0000184380

Regions

Nucleotide binding7 – 2115FAD Potential

Sites

Active site2261 By similarity
Active site2451 By similarity

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Sequences

Sequence LengthMass (Da)Tools
P38032-1 [UniParc].

Last modified October 1, 1994. Version 1.
Checksum: AF592AC55EC51259

FASTA53158,239
        10         20         30         40         50         60 
MSKKTIAVIG SGAAALSLAA AFPPSYEVTV ITKKSVKNSN SVYAQGGIAA AYAKDDSIEA 

        70         80         90        100        110        120 
HLEDTLYAGC GHNNLAIVAD VLHDGKMMVQ SLLERGFPFD RNERGGVCLG REGAHSYNRI 

       130        140        150        160        170        180 
FHAGGDATGR LLIDYLLKRI NSKIKLIENE TAADLLIEDG RCIGVMTKDS KGRLKVRHAD 

       190        200        210        220        230        240 
EVVLAAGGCG NLFLHHTNDL TVTGDGLSLA YRAGAELTDL EFTQFHPTLL VKNGVSYGLV 

       250        260        270        280        290        300 
SEAVRGEGGC LVDENGRRIM AERHPLGDLA PRDIVSRVIH EEMAKGNRVY IDFSAISDFE 

       310        320        330        340        350        360 
TRFPTITAIC EKAGIDIHSG KIPVAPGMHF LMGGVSVNRW GETTVPGLYA IGETACSGLH 

       370        380        390        400        410        420 
GANRLASNSL LEALVFGKRA AEHIIQKPVY NRQYQSGLET SVFYEVPDIE GHELQSKMTS 

       430        440        450        460        470        480 
HMSILREQSS LIELSIWLHT LPFQEVNVKD ITIRQMELSH LWQTAKLMTF SALLREESRG 

       490        500        510        520        530 
AHFRTDFPHA EVSWQGRQIV HTKKGTKIRK NEGIWNNESF TAEKITESLF S

« Hide

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References

« Hide 'large scale' references
[1]"Cloning, nucleotide sequence, and regulation of the Bacillus subtilis nadB gene and a nifS-like gene, both of which are essential for NAD biosynthesis."
Sun D., Setlow P.L.
J. Bacteriol. 175:1423-1432(1993) [PubMed: 8444804] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

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Cross-references

Sequence databases

M98822 Genomic DNA. Translation: AAA21614.1.
AL009126 Genomic DNA. Translation: CAB14747.1.
PIRC47071.
RefSeqNP_390665.1.

3D structure databases

HSSPHSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBaseSearch...

Genome annotation databases

GeneID938051.
GenomeReviewsGene locus BSU27870 in contig AL009126_GR.
KEGGbsu:BSU27870.
NMPDRfig|224308.1.peg.2790.

Organism-specific databases

SubtiListBG10867. nadB. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMP38032.
OMAP38032. CTEGPER.

Enzyme and pathway databases

BioCycBSUB224308:BSU2783-MON.
BRENDA1.4.3.16. 150.

Family and domain databases

InterProIPR003953. FAD_bind2_N.
IPR004112. Fum_Rdtase/Succ_DH_flav_C.
IPR005288. NadB.
[Graphical view]
PfamPF00890. FAD_binding_2. 1 hit.
PF02910. Succ_DH_flav_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR00551. nadB. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameNADB_BACSU
AccessionPrimary (citable) accession number: P38032
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents