P38025 (PUR7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic
EC=6.3.2.6

Alternative name(s):
SAICAR synthetase

Gene names

Name:	PUR7
Ordered Locus Names:	At3g21110
ORF Names:	MSA6.15, MSA6.26

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
Subcellular location	Plastid › chloroplast Probable.
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from genetic interaction Ref.1. Source: TAIR response to auxin stimulus Inferred from direct assay Ref.1. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from genetic interaction Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 53	53	Chloroplast Potential
	Chain	54 – 411	358	Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic		PRO_0000029873
Experimental info
	Sequence conflict	91	1	S → C in AAA16231. Ref.1
	Sequence conflict	168 – 170	3	IVS → MLY in AAA16231. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P38025 [UniParc]. Last modified December 6, 2002. Version 2. Checksum: A338C8B6E02E9A0C	FASTA	411	46,063
	10 20 30 40 50 60 MAQCVRSTLN PVRTPQSFTR KAYVKSPAFA SVSFLRAVPE FNKYPKPCSL VMSCQGKAQN 70 80 90 100 110 120 QQEERPQLSL DDLVTSNRKG EVLGTIKDSL SNCLSETNLL ATVPGLKSRI KGKVRDIYDA 130 140 150 160 170 180 GDYLVLITTD RLSAFDRNLA SIPFKGQVLN ETSLWWFNNT QHITPNAIVS SPDRNVVIAK 190 200 210 220 230 240 KCSVFPIEFV VRGYVTGSTD TSLWTVYNKG VRNYCGNELS DGLVKNQKLP ANILTPTTKA 250 260 270 280 290 300 ADHDVPISPN EIVEGGFMTQ AEFDEASMKA LSLFEFGQGV AKKHGLILVD TKYEFGRSSD 310 320 330 340 350 360 GSILLIDEIH TPDSSRYWLA GSYEERFQKG LEPENVDKEF LRLWFKENCN PYEDEVLPAA 370 380 390 400 410 PAELVTELAW RYIFLYETIT GSRIDIIPTQ EPIHDRISRN TSQALSSLRQ L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"De novo purine synthesis in Arabidopsis thaliana. II. The PUR7 gene encoding 5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole synthetase is expressed in rapidly dividing tissues." Senecoff J.F., McKinney E.C., Meagher R.B. Plant Physiol. 112:905-917(1996) [PubMed: 8938402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia. Tissue: Leaf.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed: 10907853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U05599 Unassigned DNA. Translation: AAA16231.1. AP000604 Genomic DNA. Translation: BAB01454.1. CP002686 Genomic DNA. Translation: AEE76462.1. CP002686 Genomic DNA. Translation: AEE76463.1. AY080627 mRNA. Translation: AAL85973.1. BT002734 mRNA. Translation: AAO22563.1.
IPI	IPI00548736.
RefSeq	NP_001030739.1. NM_001035662.2. NP_188748.1. NM_113005.3.
UniGene	At.43519. At.71231.
3D structure databases
ProteinModelPortal	P38025.
SMR	P38025. Positions 93-385.
ModBase	Search...
Protein-protein interaction databases
STRING	P38025.
Proteomic databases
PRIDE	P38025.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G21110.1; AT3G21110.1; AT3G21110. AT3G21110.2; AT3G21110.2; AT3G21110.
GeneID	821663.
GenomeReviews	Gene locus AT3G21110 in contig BA000014_GR.
KEGG	ath:AT3G21110.
NMPDR	fig|3702.1.peg.14342.
Organism-specific databases
TAIR	At3g21110.
Phylogenomic databases
eggNOG	KOG2835.
GeneTree	EPGT00050000018061.
HOGENOM	HBG555040.
InParanoid	P38025.
OMA	DSSRYWL.
PhylomeDB	P38025.
ProtClustDB	PLN02544.
Gene expression databases
ArrayExpress	P38025.
Genevestigator	P38025.
GermOnline	AT3G21110. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR001636. SAICAR_synth. IPR018236. SAICAR_synthetase_CS. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
KO	K01923.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF01259. SAICAR_synt. 1 hit. [Graphical view]
PROSITE	PS01057. SAICAR_SYNTHETASE_1. 1 hit. PS01058. SAICAR_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR7_ARATH

Accession

Primary (citable) accession number: P38025
Secondary accession number(s): Q8RXX4, Q9LJC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	December 6, 2002
Last modified:	January 25, 2012
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program