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Protein

Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic

Gene

PUR7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic (PUR7)
  2. Adenylosuccinate lyase (At1g36280), Adenylosuccinate lyase (AXX17_At1g37010), Adenylosuccinate lyase (At1g36280), Adenylosuccinate lyase (F15C21.8), Adenylosuccinate lyase (AXX17_At4g21680), Adenylosuccinate lyase (At4g18440)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

  • 'de novo' IMP biosynthetic process Source: GO_Central
  • response to auxin Source: TAIR

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G21110-MONOMER
UniPathwayiUPA00074; UER00131

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic (EC:6.3.2.6)
Alternative name(s):
SAICAR synthetase
Gene namesi
Name:PUR7
Ordered Locus Names:At3g21110
ORF Names:MSA6.15, MSA6.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G21110
TAIRilocus:2092975 AT3G21110

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 53ChloroplastSequence analysisAdd BLAST53
ChainiPRO_000002987354 – 411Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplasticAdd BLAST358

Proteomic databases

PaxDbiP38025
PRIDEiP38025

Expressioni

Gene expression databases

ExpressionAtlasiP38025 baseline and differential
GenevisibleiP38025 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G21110.1

Structurei

3D structure databases

ProteinModelPortaliP38025
SMRiP38025
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SAICAR synthetase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2835 Eukaryota
COG0152 LUCA
HOGENOMiHOG000230360
InParanoidiP38025
KOiK01923
OMAiEMVIRGY
OrthoDBiEOG09360D1Q
PhylomeDBiP38025

Family and domain databases

HAMAPiMF_00137 SAICAR_synth, 1 hit
InterProiView protein in InterPro
IPR028923 SAICAR_synt/ADE2_N
IPR018236 SAICAR_synthetase_CS
PfamiView protein in Pfam
PF01259 SAICAR_synt, 1 hit
PROSITEiView protein in PROSITE
PS01057 SAICAR_SYNTHETASE_1, 1 hit
PS01058 SAICAR_SYNTHETASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQCVRSTLN PVRTPQSFTR KAYVKSPAFA SVSFLRAVPE FNKYPKPCSL
60 70 80 90 100
VMSCQGKAQN QQEERPQLSL DDLVTSNRKG EVLGTIKDSL SNCLSETNLL
110 120 130 140 150
ATVPGLKSRI KGKVRDIYDA GDYLVLITTD RLSAFDRNLA SIPFKGQVLN
160 170 180 190 200
ETSLWWFNNT QHITPNAIVS SPDRNVVIAK KCSVFPIEFV VRGYVTGSTD
210 220 230 240 250
TSLWTVYNKG VRNYCGNELS DGLVKNQKLP ANILTPTTKA ADHDVPISPN
260 270 280 290 300
EIVEGGFMTQ AEFDEASMKA LSLFEFGQGV AKKHGLILVD TKYEFGRSSD
310 320 330 340 350
GSILLIDEIH TPDSSRYWLA GSYEERFQKG LEPENVDKEF LRLWFKENCN
360 370 380 390 400
PYEDEVLPAA PAELVTELAW RYIFLYETIT GSRIDIIPTQ EPIHDRISRN
410
TSQALSSLRQ L
Length:411
Mass (Da):46,063
Last modified:December 6, 2002 - v2
Checksum:iA338C8B6E02E9A0C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91S → C in AAA16231 (PubMed:8938402).Curated1
Sequence conflicti168 – 170IVS → MLY in AAA16231 (PubMed:8938402).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05599 Unassigned DNA Translation: AAA16231.1
AP000604 Genomic DNA Translation: BAB01454.1
CP002686 Genomic DNA Translation: AEE76462.1
CP002686 Genomic DNA Translation: AEE76463.1
CP002686 Genomic DNA Translation: ANM63435.1
AY080627 mRNA Translation: AAL85973.1
BT002734 mRNA Translation: AAO22563.1
RefSeqiNP_001030739.1, NM_001035662.2
NP_001319605.1, NM_001338505.1
NP_188748.1, NM_113005.4
UniGeneiAt.43519
At.71231

Genome annotation databases

EnsemblPlantsiAT3G21110.1; AT3G21110.1; AT3G21110
AT3G21110.2; AT3G21110.2; AT3G21110
AT3G21110.3; AT3G21110.3; AT3G21110
GeneIDi821663
GrameneiAT3G21110.1; AT3G21110.1; AT3G21110
AT3G21110.2; AT3G21110.2; AT3G21110
AT3G21110.3; AT3G21110.3; AT3G21110
KEGGiath:AT3G21110

Similar proteinsi

Entry informationi

Entry nameiPUR7_ARATH
AccessioniPrimary (citable) accession number: P38025
Secondary accession number(s): Q8RXX4, Q9LJC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 6, 2002
Last modified: May 23, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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