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Protein

Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic

Gene

PUR7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic (PUR7)
  2. Adenylosuccinate lyase (At1g36280), Adenylosuccinate lyase (At1g36280), Adenylosuccinate lyase (F15C21.8), Adenylosuccinate lyase (At4g18440)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G21110-MONOMER.
ARA:GQT-1240-MONOMER.
UniPathwayiUPA00074; UER00131.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic (EC:6.3.2.6)
Alternative name(s):
SAICAR synthetase
Gene namesi
Name:PUR7
Ordered Locus Names:At3g21110
ORF Names:MSA6.15, MSA6.26
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G21110.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5353ChloroplastSequence analysisAdd
BLAST
Chaini54 – 411358Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplasticPRO_0000029873Add
BLAST

Proteomic databases

PaxDbiP38025.
PRIDEiP38025.

PTM databases

iPTMnetiP38025.

Expressioni

Gene expression databases

GenevisibleiP38025. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G21110.1.

Structurei

3D structure databases

ProteinModelPortaliP38025.
SMRiP38025. Positions 111-383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SAICAR synthetase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2835. Eukaryota.
COG0152. LUCA.
HOGENOMiHOG000230360.
InParanoidiP38025.
KOiK01923.
OMAiSLFEFGQ.
PhylomeDBiP38025.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00137. SAICAR_synth.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF01259. SAICAR_synt. 1 hit.
[Graphical view]
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P38025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQCVRSTLN PVRTPQSFTR KAYVKSPAFA SVSFLRAVPE FNKYPKPCSL
60 70 80 90 100
VMSCQGKAQN QQEERPQLSL DDLVTSNRKG EVLGTIKDSL SNCLSETNLL
110 120 130 140 150
ATVPGLKSRI KGKVRDIYDA GDYLVLITTD RLSAFDRNLA SIPFKGQVLN
160 170 180 190 200
ETSLWWFNNT QHITPNAIVS SPDRNVVIAK KCSVFPIEFV VRGYVTGSTD
210 220 230 240 250
TSLWTVYNKG VRNYCGNELS DGLVKNQKLP ANILTPTTKA ADHDVPISPN
260 270 280 290 300
EIVEGGFMTQ AEFDEASMKA LSLFEFGQGV AKKHGLILVD TKYEFGRSSD
310 320 330 340 350
GSILLIDEIH TPDSSRYWLA GSYEERFQKG LEPENVDKEF LRLWFKENCN
360 370 380 390 400
PYEDEVLPAA PAELVTELAW RYIFLYETIT GSRIDIIPTQ EPIHDRISRN
410
TSQALSSLRQ L
Length:411
Mass (Da):46,063
Last modified:December 6, 2002 - v2
Checksum:iA338C8B6E02E9A0C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911S → C in AAA16231 (PubMed:8938402).Curated
Sequence conflicti168 – 1703IVS → MLY in AAA16231 (PubMed:8938402).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05599 Unassigned DNA. Translation: AAA16231.1.
AP000604 Genomic DNA. Translation: BAB01454.1.
CP002686 Genomic DNA. Translation: AEE76462.1.
CP002686 Genomic DNA. Translation: AEE76463.1.
AY080627 mRNA. Translation: AAL85973.1.
BT002734 mRNA. Translation: AAO22563.1.
RefSeqiNP_001030739.1. NM_001035662.2.
NP_188748.1. NM_113005.3.
UniGeneiAt.43519.
At.71231.

Genome annotation databases

EnsemblPlantsiAT3G21110.1; AT3G21110.1; AT3G21110.
AT3G21110.2; AT3G21110.2; AT3G21110.
GeneIDi821663.
GrameneiAT3G21110.1; AT3G21110.1; AT3G21110.
AT3G21110.2; AT3G21110.2; AT3G21110.
KEGGiath:AT3G21110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05599 Unassigned DNA. Translation: AAA16231.1.
AP000604 Genomic DNA. Translation: BAB01454.1.
CP002686 Genomic DNA. Translation: AEE76462.1.
CP002686 Genomic DNA. Translation: AEE76463.1.
AY080627 mRNA. Translation: AAL85973.1.
BT002734 mRNA. Translation: AAO22563.1.
RefSeqiNP_001030739.1. NM_001035662.2.
NP_188748.1. NM_113005.3.
UniGeneiAt.43519.
At.71231.

3D structure databases

ProteinModelPortaliP38025.
SMRiP38025. Positions 111-383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G21110.1.

PTM databases

iPTMnetiP38025.

Proteomic databases

PaxDbiP38025.
PRIDEiP38025.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G21110.1; AT3G21110.1; AT3G21110.
AT3G21110.2; AT3G21110.2; AT3G21110.
GeneIDi821663.
GrameneiAT3G21110.1; AT3G21110.1; AT3G21110.
AT3G21110.2; AT3G21110.2; AT3G21110.
KEGGiath:AT3G21110.

Organism-specific databases

TAIRiAT3G21110.

Phylogenomic databases

eggNOGiKOG2835. Eukaryota.
COG0152. LUCA.
HOGENOMiHOG000230360.
InParanoidiP38025.
KOiK01923.
OMAiSLFEFGQ.
PhylomeDBiP38025.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00131.
BioCyciARA:AT3G21110-MONOMER.
ARA:GQT-1240-MONOMER.

Miscellaneous databases

PROiP38025.

Gene expression databases

GenevisibleiP38025. AT.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00137. SAICAR_synth.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF01259. SAICAR_synt. 1 hit.
[Graphical view]
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "De novo purine synthesis in Arabidopsis thaliana. II. The PUR7 gene encoding 5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole synthetase is expressed in rapidly dividing tissues."
    Senecoff J.F., McKinney E.C., Meagher R.B.
    Plant Physiol. 112:905-917(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
    Tissue: Leaf.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiPUR7_ARATH
AccessioniPrimary (citable) accession number: P38025
Secondary accession number(s): Q8RXX4, Q9LJC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: December 6, 2002
Last modified: February 17, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.