P38024 (PUR6_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Multifunctional protein ADE2

Including the following 2 domains:

1. Phosphoribosylaminoimidazole-succinocarboxamide synthase
   EC=6.3.2.6
   Alternative name(s):
   SAICAR synthetase
2. Phosphoribosylaminoimidazole carboxylase
   EC=4.1.1.21
   Alternative name(s):
   AIR carboxylase
   Short name=AIRC

Gene names

Name:

AIRC

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homooctamer By similarity.
Sequence similarities	In the N-terminal section; belongs to the SAICAR synthetase family. In the C-terminal section; belongs to the AIR carboxylase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Ligase Lyase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Multifunctional protein ADE2		PRO_0000075029
Regions
	Region	1 – 261	261	SAICAR synthetase
	Region	262 – 426	165	AIR carboxylase

Sequences

	Sequence		Length	Mass (Da)
	P38024 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: B4A990AEB2AD360C	FASTA	426	47,240
	10 20 30 40 50 60 MAPAASELKL GKKVNEGKTK EVYELPDIPG CVLMQSKDQI TAGNAARKDR MEGKAAISNT 70 80 90 100 110 120 TTSCVFQLLQ EAGIKTAFVR KQSDTAFIAA HCEMIPIEWV CRRIATGSFL KRNPGVKEGY 130 140 150 160 170 180 KFYPPKIEMF YKDDANNDPQ WSEEQLIEAK FSFAGLTIGK TEVDIMARST QAIFEILEKS 190 200 210 220 230 240 WQPQNCTLVD LKIEFGVNIL TKEIVLADVI DNDSWRLWPS GDRSQQKDKQ SYRDLKEVTP 250 260 270 280 290 300 EALQMVKRNF EWVAERVELL LKTKSQGRVV VLMGSTSDLG HCEKIKKACA TFGIPCELRV 310 320 330 340 350 360 TSAHKGPDET LRIKAEYEGD GIPTVFVAVA GRSNGLGPVM SGNTAYPVVN CPPLSSDWGA 370 380 390 400 410 420 QDVWSSLRLP SGLGCPTTLS PEGAAQFAAQ IFGLNNHLVW AKLRSNMLNT WISLKQADKK LRECTL

References

[1]	"Cloning of a chicken liver cDNA encoding 5-aminoimidazole ribonucleotide carboxylase and 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase by functional complementation of Escherichia coli pur mutants." Chen Z.D., Dixon J.E., Zalkin H. Proc. Natl. Acad. Sci. U.S.A. 87:3097-3101(1990) [PubMed: 1691501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Coexpression of two closely linked avian genes for purine nucleotide synthesis from a bidirectional promoter." Gavalas A., Dixon J.E., Brayton K.A., Zalkin H. Mol. Cell. Biol. 13:4784-4792(1993) [PubMed: 8336716] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M31764 mRNA. Translation: AAA48601.1. L12533 Genomic DNA. Translation: AAA17894.1.
IPI	IPI00602224.
PIR	A35641.
RefSeq	NP_990855.1. NM_205524.2.
UniGene	Gga.3058.
3D structure databases
ProteinModelPortal	P38024.
SMR	P38024. Positions 8-426.
ModBase	Search...
Protein-protein interaction databases
STRING	P38024.
Proteomic databases
PRIDE	P38024.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000022308; ENSGALP00000022269; ENSGALG00000013726.
GeneID	396534.
KEGG	gga:396534.
Organism-specific databases
CTD	10606.
Phylogenomic databases
eggNOG	veNOG07150.
GeneTree	ENSGT00390000010172.
HOGENOM	HBG306070.
HOVERGEN	HBG008335.
InParanoid	P38024.
OMA	QCRVVVL.
OrthoDB	EOG44BB27.
PhylomeDB	P38024.
Family and domain databases
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR000031. N5-CAIR_Mutase_PurE_dom. IPR001636. SAICAR_synth. IPR018236. SAICAR_synthetase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
KO	K01587.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF00731. AIRC. 1 hit. PF01259. SAICAR_synt. 1 hit. [Graphical view]
SMART	SM01001. AIRC. 1 hit. [Graphical view]
SUPFAM	SSF52255. AIR_carboxyl. 1 hit.
TIGRFAMs	TIGR01162. PurE. 1 hit.
PROSITE	PS01057. SAICAR_SYNTHETASE_1. 1 hit. PS01058. SAICAR_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR6_CHICK

Accession

Primary (citable) accession number: P38024
Secondary accession number(s): P87465

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program