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P38009

- PUR92_YEAST

UniProt

P38009 - PUR92_YEAST

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Protein

Bifunctional purine biosynthesis protein ADE17

Gene

ADE17

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

Pathwayi

GO - Molecular functioni

  1. IMP cyclohydrolase activity Source: SGD
  2. phosphoribosylaminoimidazolecarboxamide formyltransferase activity Source: SGD

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: SGD
  2. purine nucleotide biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transferase

Keywords - Biological processi

Purine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:YMR120C-MONOMER.
YEAST:YMR120C-MONOMER.
UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein ADE17
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC:2.1.2.3)
Alternative name(s):
5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
AICAR transformylase
IMP cyclohydrolase (EC:3.5.4.10)
Alternative name(s):
ATIC
IMP synthase
Inosinicase
Gene namesi
Name:ADE17
Ordered Locus Names:YMR120C
ORF Names:YM8564.02C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR120c.
SGDiS000004727. ADE17.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 592592Bifunctional purine biosynthesis protein ADE17PRO_0000192160Add
BLAST

Proteomic databases

MaxQBiP38009.
PaxDbiP38009.
PeptideAtlasiP38009.
PRIDEiP38009.

Expressioni

Gene expression databases

GenevestigatoriP38009.

Interactioni

Subunit structurei

Homodimer (Possible).

Binary interactionsi

WithEntry#Exp.IntActNotes
ADE16P541133EBI-14223,EBI-14213

Protein-protein interaction databases

BioGridi35297. 62 interactions.
DIPiDIP-6288N.
IntActiP38009. 28 interactions.
MINTiMINT-696423.
STRINGi4932.YMR120C.

Structurei

3D structure databases

ProteinModelPortaliP38009.
SMRiP38009. Positions 6-592.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.By similarity

Sequence similaritiesi

Belongs to the PurH family.Curated

Phylogenomic databases

eggNOGiCOG0138.
GeneTreeiENSGT00390000004553.
HOGENOMiHOG000230372.
InParanoidiP38009.
KOiK00602.
OMAiCNLKGIS.
OrthoDBiEOG7HXD0J.

Family and domain databases

Gene3Di1.10.287.440. 1 hit.
3.40.140.20. 3 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR024050. AICAR_Tfase_insert_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.

Sequencei

Sequence statusi: Complete.

P38009-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANYTKTAIL SVYDKTGLLD LARGLIEKNV RILASGGTAR MIRDAGFPIE
60 70 80 90 100
DVSAITHAPE MLGGRVKTLH PAVHGGILAR DIDSDEKDLK EQHIEKVDYV
110 120 130 140 150
VCNLYPFKET VAKVGVTIPE AVEEIDIGGV TLLRAAAKNH ARVTILSDPK
160 170 180 190 200
DYSEFLSELS SNGEISQDLR NRLALKAFEH TADYDAAISD FFRKQYSEGQ
210 220 230 240 250
AQITLRYGAN PHQKPAQAYV SQQDSLPFKV LCGSPGYINL LDALNSWPLV
260 270 280 290 300
KELSASLNLP AAASFKHVSP AGAAVGIPLS DVEKQVYFVA DIENLSPLAC
310 320 330 340 350
AYARARGADR MSSFGDWIAL SNIVDVPTAK IISREVSDGV IAPGYEPEAL
360 370 380 390 400
AILSKKKGGK YCILQIDPNY VPEAVERRQV YGVTLEQKRN DAIINQSTFK
410 420 430 440 450
EIVSQNKNLT EQAIIDLTVA TIAIKYTQSN SVCYARNGMV VGLGAGQQSR
460 470 480 490 500
IHCTRLAGDK ADNWWFRQHP RVLEIKWAKG VKRPEKSNAI DLFVTGQIPT
510 520 530 540 550
EEPELSEYQS KFEEIPKPFT PEERKEWLSK LTNVSLSSDA FFPFPDNVYR
560 570 580 590
AVKSGVKYIA APSGSVMDKV VFSAADSFDL VYVENPIRLF HH
Length:592
Mass (Da):65,263
Last modified:October 1, 1996 - v2
Checksum:i8ABA71761B512242
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti389 – 3891R → A AA sequence (PubMed:7895733)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49273 Genomic DNA. Translation: CAA89269.1.
BK006946 Genomic DNA. Translation: DAA10017.1.
PIRiS54489.
RefSeqiNP_013839.1. NM_001182621.1.

Genome annotation databases

EnsemblFungiiYMR120C; YMR120C; YMR120C.
GeneIDi855149.
KEGGisce:YMR120C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49273 Genomic DNA. Translation: CAA89269.1 .
BK006946 Genomic DNA. Translation: DAA10017.1 .
PIRi S54489.
RefSeqi NP_013839.1. NM_001182621.1.

3D structure databases

ProteinModelPortali P38009.
SMRi P38009. Positions 6-592.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35297. 62 interactions.
DIPi DIP-6288N.
IntActi P38009. 28 interactions.
MINTi MINT-696423.
STRINGi 4932.YMR120C.

Proteomic databases

MaxQBi P38009.
PaxDbi P38009.
PeptideAtlasi P38009.
PRIDEi P38009.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR120C ; YMR120C ; YMR120C .
GeneIDi 855149.
KEGGi sce:YMR120C.

Organism-specific databases

CYGDi YMR120c.
SGDi S000004727. ADE17.

Phylogenomic databases

eggNOGi COG0138.
GeneTreei ENSGT00390000004553.
HOGENOMi HOG000230372.
InParanoidi P38009.
KOi K00602.
OMAi CNLKGIS.
OrthoDBi EOG7HXD0J.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00133 .
UPA00074 ; UER00135 .
BioCyci MetaCyc:YMR120C-MONOMER.
YEAST:YMR120C-MONOMER.

Miscellaneous databases

NextBioi 978551.

Gene expression databases

Genevestigatori P38009.

Family and domain databases

Gene3Di 1.10.287.440. 1 hit.
3.40.140.20. 3 hits.
3.40.50.1380. 1 hit.
HAMAPi MF_00139. PurH.
InterProi IPR024051. AICAR_Tfase_dom.
IPR024050. AICAR_Tfase_insert_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view ]
PANTHERi PTHR11692. PTHR11692. 1 hit.
Pfami PF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view ]
PIRSFi PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTi SM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view ]
SUPFAMi SSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsi TIGR00355. purH. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: PROTEIN SEQUENCE OF 140-150 AND 389-400.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPUR92_YEAST
AccessioniPrimary (citable) accession number: P38009
Secondary accession number(s): D6VZU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 60900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3