Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P38009

- PUR92_YEAST

UniProt

P38009 - PUR92_YEAST

Protein

Bifunctional purine biosynthesis protein ADE17

Gene

ADE17

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.
    IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.

    Pathwayi

    GO - Molecular functioni

    1. IMP cyclohydrolase activity Source: SGD
    2. phosphoribosylaminoimidazolecarboxamide formyltransferase activity Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: SGD
    2. purine nucleotide biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:YMR120C-MONOMER.
    YEAST:YMR120C-MONOMER.
    UniPathwayiUPA00074; UER00133.
    UPA00074; UER00135.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional purine biosynthesis protein ADE17
    Including the following 2 domains:
    Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC:2.1.2.3)
    Alternative name(s):
    5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase
    AICAR transformylase
    IMP cyclohydrolase (EC:3.5.4.10)
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
    Gene namesi
    Name:ADE17
    Ordered Locus Names:YMR120C
    ORF Names:YM8564.02C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR120c.
    SGDiS000004727. ADE17.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 592592Bifunctional purine biosynthesis protein ADE17PRO_0000192160Add
    BLAST

    Proteomic databases

    MaxQBiP38009.
    PaxDbiP38009.
    PeptideAtlasiP38009.
    PRIDEiP38009.

    Expressioni

    Gene expression databases

    GenevestigatoriP38009.

    Interactioni

    Subunit structurei

    Homodimer (Possible).

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ADE16P541133EBI-14223,EBI-14213

    Protein-protein interaction databases

    BioGridi35297. 60 interactions.
    DIPiDIP-6288N.
    IntActiP38009. 28 interactions.
    MINTiMINT-696423.
    STRINGi4932.YMR120C.

    Structurei

    3D structure databases

    ProteinModelPortaliP38009.
    SMRiP38009. Positions 6-592.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The IMP cyclohydrolase activity resides in the N-terminal region.By similarity

    Sequence similaritiesi

    Belongs to the PurH family.Curated

    Phylogenomic databases

    eggNOGiCOG0138.
    HOGENOMiHOG000230372.
    KOiK00602.
    OMAiCNLKGIS.
    OrthoDBiEOG7HXD0J.

    Family and domain databases

    Gene3Di1.10.287.440. 1 hit.
    3.40.140.20. 3 hits.
    3.40.50.1380. 1 hit.
    HAMAPiMF_00139. PurH.
    InterProiIPR024051. AICAR_Tfase_dom.
    IPR024050. AICAR_Tfase_insert_dom.
    IPR002695. AICARFT_IMPCHas.
    IPR016193. Cytidine_deaminase-like.
    IPR011607. MGS-like_dom.
    [Graphical view]
    PANTHERiPTHR11692. PTHR11692. 1 hit.
    PfamiPF01808. AICARFT_IMPCHas. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
    SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52335. SSF52335. 1 hit.
    SSF53927. SSF53927. 1 hit.
    TIGRFAMsiTIGR00355. purH. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P38009-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANYTKTAIL SVYDKTGLLD LARGLIEKNV RILASGGTAR MIRDAGFPIE    50
    DVSAITHAPE MLGGRVKTLH PAVHGGILAR DIDSDEKDLK EQHIEKVDYV 100
    VCNLYPFKET VAKVGVTIPE AVEEIDIGGV TLLRAAAKNH ARVTILSDPK 150
    DYSEFLSELS SNGEISQDLR NRLALKAFEH TADYDAAISD FFRKQYSEGQ 200
    AQITLRYGAN PHQKPAQAYV SQQDSLPFKV LCGSPGYINL LDALNSWPLV 250
    KELSASLNLP AAASFKHVSP AGAAVGIPLS DVEKQVYFVA DIENLSPLAC 300
    AYARARGADR MSSFGDWIAL SNIVDVPTAK IISREVSDGV IAPGYEPEAL 350
    AILSKKKGGK YCILQIDPNY VPEAVERRQV YGVTLEQKRN DAIINQSTFK 400
    EIVSQNKNLT EQAIIDLTVA TIAIKYTQSN SVCYARNGMV VGLGAGQQSR 450
    IHCTRLAGDK ADNWWFRQHP RVLEIKWAKG VKRPEKSNAI DLFVTGQIPT 500
    EEPELSEYQS KFEEIPKPFT PEERKEWLSK LTNVSLSSDA FFPFPDNVYR 550
    AVKSGVKYIA APSGSVMDKV VFSAADSFDL VYVENPIRLF HH 592
    Length:592
    Mass (Da):65,263
    Last modified:October 1, 1996 - v2
    Checksum:i8ABA71761B512242
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti389 – 3891R → A AA sequence (PubMed:7895733)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49273 Genomic DNA. Translation: CAA89269.1.
    BK006946 Genomic DNA. Translation: DAA10017.1.
    PIRiS54489.
    RefSeqiNP_013839.1. NM_001182621.1.

    Genome annotation databases

    EnsemblFungiiYMR120C; YMR120C; YMR120C.
    GeneIDi855149.
    KEGGisce:YMR120C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49273 Genomic DNA. Translation: CAA89269.1 .
    BK006946 Genomic DNA. Translation: DAA10017.1 .
    PIRi S54489.
    RefSeqi NP_013839.1. NM_001182621.1.

    3D structure databases

    ProteinModelPortali P38009.
    SMRi P38009. Positions 6-592.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35297. 60 interactions.
    DIPi DIP-6288N.
    IntActi P38009. 28 interactions.
    MINTi MINT-696423.
    STRINGi 4932.YMR120C.

    Proteomic databases

    MaxQBi P38009.
    PaxDbi P38009.
    PeptideAtlasi P38009.
    PRIDEi P38009.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR120C ; YMR120C ; YMR120C .
    GeneIDi 855149.
    KEGGi sce:YMR120C.

    Organism-specific databases

    CYGDi YMR120c.
    SGDi S000004727. ADE17.

    Phylogenomic databases

    eggNOGi COG0138.
    HOGENOMi HOG000230372.
    KOi K00602.
    OMAi CNLKGIS.
    OrthoDBi EOG7HXD0J.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00133 .
    UPA00074 ; UER00135 .
    BioCyci MetaCyc:YMR120C-MONOMER.
    YEAST:YMR120C-MONOMER.

    Miscellaneous databases

    NextBioi 978551.

    Gene expression databases

    Genevestigatori P38009.

    Family and domain databases

    Gene3Di 1.10.287.440. 1 hit.
    3.40.140.20. 3 hits.
    3.40.50.1380. 1 hit.
    HAMAPi MF_00139. PurH.
    InterProi IPR024051. AICAR_Tfase_dom.
    IPR024050. AICAR_Tfase_insert_dom.
    IPR002695. AICARFT_IMPCHas.
    IPR016193. Cytidine_deaminase-like.
    IPR011607. MGS-like_dom.
    [Graphical view ]
    PANTHERi PTHR11692. PTHR11692. 1 hit.
    Pfami PF01808. AICARFT_IMPCHas. 1 hit.
    PF02142. MGS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000414. AICARFT_IMPCHas. 1 hit.
    SMARTi SM00798. AICARFT_IMPCHas. 1 hit.
    SM00851. MGS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52335. SSF52335. 1 hit.
    SSF53927. SSF53927. 1 hit.
    TIGRFAMsi TIGR00355. purH. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: PROTEIN SEQUENCE OF 140-150 AND 389-400.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPUR92_YEAST
    AccessioniPrimary (citable) accession number: P38009
    Secondary accession number(s): D6VZU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 138 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 60900 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3