P37981 (IPYR_THEAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	Ta0399

Organism

Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

Taxonomic identifier

273075 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subunit structure	Homohexamer.
Subcellular location	Cytoplasm HAMAP MF_00209.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137562
Sites
	Metal binding	67	1	Magnesium 1 By similarity
	Metal binding	72	1	Magnesium 1 By similarity
	Metal binding	72	1	Magnesium 2 By similarity
	Metal binding	104	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P37981 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: B4210114AFABE422	FASTA	179	20,465
	10 20 30 40 50 60 MESFYHSVPV GPKPPEEVYV IVEIPRGSRV KYEIAKDFPG MLVDRVLYSS VVYPVDYGLI 70 80 90 100 110 120 PRTLYYDGDP MDVMVLISQP TFPGAIMKVR PIGMMKMVDQ GETDNKILAV FDKDPNVSYI 130 140 150 160 170 KDLKDVNAHL LDEIANFFST YKILEKKETK VLGWEGKEAA LKEIEVSIKM YEEKYGKKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the gene for the cytoplasmic inorganic pyrophosphatase from the thermoacidophilic archaebacterium Thermoplasma acidophilum." Richter O.-M.H., Schaefer G. Eur. J. Biochem. 209:351-355(1992) [PubMed: 1327775] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
[2]	"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165.
[3]	"Purification and enzymic characterization of the cytoplasmic pyrophosphatase from the thermoacidophilic archaebacterium Thermoplasma acidophilum." Richter O.-M.H., Schaefer G. Eur. J. Biochem. 209:343-349(1992) [PubMed: 1327774] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-22, CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64200 Genomic DNA. Translation: CAA45525.1. AL445064 Genomic DNA. Translation: CAC11542.1.
PIR	S29313.
RefSeq	NP_393878.1. NC_002578.1.
3D structure databases
ProteinModelPortal	P37981.
SMR	P37981. Positions 5-174.
ModBase	Search...
Proteomic databases
PRIDE	P37981.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1456011.
GenomeReviews	Gene locus Ta0399 in contig AL139299_GR.
KEGG	tac:Ta0399.
NMPDR	fig|273075.1.peg.400.
Phylogenomic databases
HOGENOM	HBG529150.
OMA	SESAHEA.
PhylomeDB	P37981.
ProtClustDB	CLSK227943.
Enzyme and pathway databases
BioCyc	TACI273075:TA0399-MONOMER.
Family and domain databases
HAMAP	MF_00209. Inorganic_PPase. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_THEAC

Accession

Primary (citable) accession number: P37981

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program